Bioprospection and characterization of cellulase-producing bacteria from Brazilian Cerrado soil
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/17426 |
Resumo: | This work describes the bioprospection and characterization of cellulase-producing bacteria isolated from the soil obtained in a preserved region of the Cerrado biome, in Uberaba, Brazil. Cellulolytic activity was confirmed in 14 of the 84 bacterial strains isolated. According to enzymatic quantification, five strains were selected as the best cellulase producers, and based on the partial sequencing of the 16S rRNA gene the strains were identified as Bacillus siamensis (isolate AB-9;5.000U/mL), Bacillus toyonensis (isolate AB-1;4.630U/mL), Bacillus methylotrophicus (isolates MB-3;4.236U/mL and MP-7;4.282U/mL), and Bacillus drentensis (isolate ME-2;4.444U/mL). The enzymatic extract obtained from B. siamensis AB-9 was the most stable in different pH values (2-8), maintaining its cellulolytic activity, while B. toyonensis AB-1 and B. methylotrophicus MP-7 produced cellulases with maximum activity at pH 7 and 8. The cellulases produced by B. siamensis AB-9 and B. toyonensis AB-1 showed high enzymatic activity at all temperatures analyzed (10-80°C), while B. methylotrophicus MB-3 cellulases had maximum activity in the range of 20-70°C. To our knowledge, this is the first time that cellulase-producing bacteria isolated from Brazilian Cerrado biome in the region of Triângulo Mineiro with potential biotechnological application are described. |
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Research, Society and Development |
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Bioprospection and characterization of cellulase-producing bacteria from Brazilian Cerrado soilBioprospección y caracterización de bacterias productoras de celulasa del suelo del Cerrado brasileñoBioprospecção e caracterização de bactérias produtoras de celulase a partir do solo do Cerrado brasileiroScreeningEnzimaBacillusBiotecnologia.ScreeningEnzimaBacillusBiotecnología.ScreeningEnzymeBacillusBiotechnology.This work describes the bioprospection and characterization of cellulase-producing bacteria isolated from the soil obtained in a preserved region of the Cerrado biome, in Uberaba, Brazil. Cellulolytic activity was confirmed in 14 of the 84 bacterial strains isolated. According to enzymatic quantification, five strains were selected as the best cellulase producers, and based on the partial sequencing of the 16S rRNA gene the strains were identified as Bacillus siamensis (isolate AB-9;5.000U/mL), Bacillus toyonensis (isolate AB-1;4.630U/mL), Bacillus methylotrophicus (isolates MB-3;4.236U/mL and MP-7;4.282U/mL), and Bacillus drentensis (isolate ME-2;4.444U/mL). The enzymatic extract obtained from B. siamensis AB-9 was the most stable in different pH values (2-8), maintaining its cellulolytic activity, while B. toyonensis AB-1 and B. methylotrophicus MP-7 produced cellulases with maximum activity at pH 7 and 8. The cellulases produced by B. siamensis AB-9 and B. toyonensis AB-1 showed high enzymatic activity at all temperatures analyzed (10-80°C), while B. methylotrophicus MB-3 cellulases had maximum activity in the range of 20-70°C. To our knowledge, this is the first time that cellulase-producing bacteria isolated from Brazilian Cerrado biome in the region of Triângulo Mineiro with potential biotechnological application are described.Este trabajo describe la bioprospección y caracterización de bacterias productoras de celulasa aisladas de suelo obtenido en una región preservada del bioma Cerrado, en Uberaba, Brasil. Se confirmó la actividad celulolítica en 14 de las 84 cepas bacterianas aisladas. De acuerdo con la cuantificación enzimática, se seleccionaron cinco aislamientos como los mejores productores de celulasa y en base a la secuenciación parcial del gen rRNA 16S se identificaron como Bacillus siamensis (aislado AB-9; 5,000U/mL), Bacillus toyonensis (aislado AB-1; 4.630U/mL), Bacillus methylotrophicus (aislados MB-3; 4.236U/mL y MP-7; 4.282U/mL) y Bacillus drentensis (aislado ME-2; 4.444U/mL). El extracto enzimático obtenido de B. siamensis AB-9 fue el más estable a diferentes valores de pH (2-8), manteniendo su actividad celulolítica, mientras que B. toyonensis AB-1 y B. methylotrophicus MP-7 produjeron celulasas con máximos actividad a pH 7 y 8. Las celulasas producidas por B. siamensis AB-9 y B. toyonensis AB-1 mostraron alta actividad enzimática a todas las temperaturas analizadas (10-80°C), mientras que las celulasas de B. methylotrophicus MB-3 mostró una actividad máxima en el rango de 20-70°C. Hasta el momento, esta es la primera vez que se describen bacterias productoras de celulasa aisladas del bioma del Cerrado brasileño en la región del Triângulo Mineiro con potenciales aplicaciones biotecnológicas.Este trabalho descreve a bioprospecção e a caracterização de bactérias produtoras de celulase isoladas de solo obtido em uma região preservada do bioma Cerrado, em Uberaba, Brasil. A atividade celulolítica foi confirmada em 14 das 84 linhagens bacterianas isoladas. De acordo com a quantificação enzimática, cinco isolados foram selecionados como os melhores produtores de celulase e com base no sequenciamento parcial do gene 16S rRNA foram identificadas como Bacillus siamensis (isolado AB-9; 5.000U/mL), Bacillus toyonensis (isolado AB-1; 4.630U/mL), Bacillus methylotrophicus (isolados MB-3; 4.236U/mL e MP-7; 4.282U/mL) e Bacillus drentensis (isolado ME-2; 4.444U/mL). O extrato enzimático obtido de B. siamensis AB-9 foi o mais estável em diferentes valores de pH (2-8), mantendo sua atividade celulolítica, enquanto B. toyonensis AB-1 e B. methylotrophicus MP-7 produziram celulases com atividade máxima em pH 7 e 8. As celulases produzidas por B. siamensis AB-9 e B. toyonensis AB-1 apresentaram alta atividade enzimática em todas as temperaturas analisadas (10-80°C), enquanto as celulases de B. methylotrophicus MB-3 apresentaram atividade máxima na faixa de 20-70°C. Até o momento, esta é a primeira vez que bactérias produtoras de celulase isoladas do bioma Cerrado brasileiro na região do Triângulo Mineiro com potencial aplicação biotecnológica são descritas.Research, Society and Development2021-07-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1742610.33448/rsd-v10i8.17426Research, Society and Development; Vol. 10 No. 8; e34010817426Research, Society and Development; Vol. 10 Núm. 8; e34010817426Research, Society and Development; v. 10 n. 8; e340108174262525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/17426/15554Copyright (c) 2021 Bianca Aguiar Alves; Isabela Sguilla Rotta; Alessandra Barbosa Ferreira-Machado; Aline Dias Paivahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAlves, Bianca Aguiar Rotta, Isabela Sguilla Ferreira-Machado, Alessandra Barbosa Paiva, Aline Dias2021-08-21T18:46:59Zoai:ojs.pkp.sfu.ca:article/17426Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:37:45.952076Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Bioprospection and characterization of cellulase-producing bacteria from Brazilian Cerrado soil Bioprospección y caracterización de bacterias productoras de celulasa del suelo del Cerrado brasileño Bioprospecção e caracterização de bactérias produtoras de celulase a partir do solo do Cerrado brasileiro |
title |
Bioprospection and characterization of cellulase-producing bacteria from Brazilian Cerrado soil |
spellingShingle |
Bioprospection and characterization of cellulase-producing bacteria from Brazilian Cerrado soil Alves, Bianca Aguiar Screening Enzima Bacillus Biotecnologia. Screening Enzima Bacillus Biotecnología. Screening Enzyme Bacillus Biotechnology. |
title_short |
Bioprospection and characterization of cellulase-producing bacteria from Brazilian Cerrado soil |
title_full |
Bioprospection and characterization of cellulase-producing bacteria from Brazilian Cerrado soil |
title_fullStr |
Bioprospection and characterization of cellulase-producing bacteria from Brazilian Cerrado soil |
title_full_unstemmed |
Bioprospection and characterization of cellulase-producing bacteria from Brazilian Cerrado soil |
title_sort |
Bioprospection and characterization of cellulase-producing bacteria from Brazilian Cerrado soil |
author |
Alves, Bianca Aguiar |
author_facet |
Alves, Bianca Aguiar Rotta, Isabela Sguilla Ferreira-Machado, Alessandra Barbosa Paiva, Aline Dias |
author_role |
author |
author2 |
Rotta, Isabela Sguilla Ferreira-Machado, Alessandra Barbosa Paiva, Aline Dias |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Alves, Bianca Aguiar Rotta, Isabela Sguilla Ferreira-Machado, Alessandra Barbosa Paiva, Aline Dias |
dc.subject.por.fl_str_mv |
Screening Enzima Bacillus Biotecnologia. Screening Enzima Bacillus Biotecnología. Screening Enzyme Bacillus Biotechnology. |
topic |
Screening Enzima Bacillus Biotecnologia. Screening Enzima Bacillus Biotecnología. Screening Enzyme Bacillus Biotechnology. |
description |
This work describes the bioprospection and characterization of cellulase-producing bacteria isolated from the soil obtained in a preserved region of the Cerrado biome, in Uberaba, Brazil. Cellulolytic activity was confirmed in 14 of the 84 bacterial strains isolated. According to enzymatic quantification, five strains were selected as the best cellulase producers, and based on the partial sequencing of the 16S rRNA gene the strains were identified as Bacillus siamensis (isolate AB-9;5.000U/mL), Bacillus toyonensis (isolate AB-1;4.630U/mL), Bacillus methylotrophicus (isolates MB-3;4.236U/mL and MP-7;4.282U/mL), and Bacillus drentensis (isolate ME-2;4.444U/mL). The enzymatic extract obtained from B. siamensis AB-9 was the most stable in different pH values (2-8), maintaining its cellulolytic activity, while B. toyonensis AB-1 and B. methylotrophicus MP-7 produced cellulases with maximum activity at pH 7 and 8. The cellulases produced by B. siamensis AB-9 and B. toyonensis AB-1 showed high enzymatic activity at all temperatures analyzed (10-80°C), while B. methylotrophicus MB-3 cellulases had maximum activity in the range of 20-70°C. To our knowledge, this is the first time that cellulase-producing bacteria isolated from Brazilian Cerrado biome in the region of Triângulo Mineiro with potential biotechnological application are described. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/17426 10.33448/rsd-v10i8.17426 |
url |
https://rsdjournal.org/index.php/rsd/article/view/17426 |
identifier_str_mv |
10.33448/rsd-v10i8.17426 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/17426/15554 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 8; e34010817426 Research, Society and Development; Vol. 10 Núm. 8; e34010817426 Research, Society and Development; v. 10 n. 8; e34010817426 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052807523598336 |