Validation of renal and hepatic profile parameters of rodents Rattus novergicus kept in the maintenance vivarium of the University of Franca

Detalhes bibliográficos
Autor(a) principal: Almeida Junior, Silvio de
Data de Publicação: 2020
Outros Autores: Pereira, Poliana Marques, Totoli, Vanessa de Souza, Carrijo, Micaela Silva, Badoco, Fernanda Rafacho, Pereira, Lucas de Freitas, Dias, Fernanda Gosuen Gonçalves, Furtado, Ricardo Andrade
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/2619
Resumo: Due to the high use of animals as experimental models for pharmaceutical trials, especially rats, it is necessary to standardize reference values for the evaluation of liver perfil by the analytes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and renal function, through the analytes urea and creatinine. For this purpose, samples were collected from 169 samples of Rattus norvegicus of Wistar strain, from the Vivarium of the University of Franca (SP), provided of semi-automated analytical methodology. For the calculation of the value, mean and standard deviation were performed for standardization and for the creation of reference values, therefore -2DP for minimum value determination and + 2DP for maximum value were used. The liver enzyme ALT presented a reference range from 21 to 96 U / L, while the AST from 44 to 186 U / L. In renal markers, urea was 20 to 73 mg / dL and creatinine 0.23 to 0.77 mg / dL. Given the applied methodology and the results obtained, it is admitted that it is very important to standardize the reference values of analytes in animal houses due to geographical changes, protocols, diets, among others; In addition, reducing the use of animals in experimental procedures in addition to guiding researchers on animal health and assisting analysis in various experimental research projects.
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spelling Validation of renal and hepatic profile parameters of rodents Rattus novergicus kept in the maintenance vivarium of the University of FrancaValidación de los parámetros de perfil renal y hepático de roedores Rattus norvegicus mantenidos en el vivero de mantenimiento de la Universidad de FrancaValidação de parâmetros do perfil renal e hepático de roedores Rattus norvegicus do Biotério de manutenção da Universidade de Francabioquímica clínicabiomarcadoresbiotériosenzimas séricasratos.bioquímica clínicabiomarcadoresviverosenzimas séricasratasclinical biochemistrybiomarkervivariumserum enzymesratsDue to the high use of animals as experimental models for pharmaceutical trials, especially rats, it is necessary to standardize reference values for the evaluation of liver perfil by the analytes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and renal function, through the analytes urea and creatinine. For this purpose, samples were collected from 169 samples of Rattus norvegicus of Wistar strain, from the Vivarium of the University of Franca (SP), provided of semi-automated analytical methodology. For the calculation of the value, mean and standard deviation were performed for standardization and for the creation of reference values, therefore -2DP for minimum value determination and + 2DP for maximum value were used. The liver enzyme ALT presented a reference range from 21 to 96 U / L, while the AST from 44 to 186 U / L. In renal markers, urea was 20 to 73 mg / dL and creatinine 0.23 to 0.77 mg / dL. Given the applied methodology and the results obtained, it is admitted that it is very important to standardize the reference values of analytes in animal houses due to geographical changes, protocols, diets, among others; In addition, reducing the use of animals in experimental procedures in addition to guiding researchers on animal health and assisting analysis in various experimental research projects.Debido al alto uso de animales como modelos experimentales para ensayos farmacéuticos, principalmente ratas, es necesario estandarizar los valores de referencia para la evaluación del perfil hepático por los analitos alanina aminotransferasa (ALT) y aspartato aminotransferasa (AST) y la función renal, a través de los analitos de urea y creatinina . Para ello, se recogieron muestras de 169 muestras de Rattus norvegicus de la espécie Wistar, del Vivarium de la Universidad de Franca (SP), realizadas con metodología analítica semiautomatizada. Para el cálculo del valor, se realizaron promedios y desviaciones estándar para la estandarización y la creación de valores de referencia, utilizando -2DP para determinar el valor mínimo y + 2DP para el valor máximo. La enzima hepática ALT mostró un rango de referencia de 21 a 96 U / L, mientras que la AST de 44 a 186 U / L. En los marcadores renales, la urea fue de 20 a 73 mg / dL y la creatinina de 0.23 a 0,77 mg / dL. Dada la metodología aplicada y los resultados obtenidos, se admite que es muy importante estandarizar los valores de referencia de analitos en viveros animales debido a cambios geográficos, protocolos, dietas, entre otros, además de reducir el uso de animales en procedimientos experimentales. , además de guiar a los investigadores en salud animal y ayudar al análisis en varios proyectos de investigación experimental.Devido ao alto uso de animais como modelos experimentais para ensaios farmacêuticos, principalmente ratos, é necessário padronizar valores de referência para avaliação do perfil hepática pelos analitos alanina aminotransferase (ALT) e aspartato aminotransferase (AST) e função renal, através dos analitos uréia e creatinina. Para tanto, foram coletadas amostras de 169 amostras de Rattus norvegicus da linhagem Wistar, do Biotério da Universidade de Franca (SP), realizados com metodologia analítica semi-automatizado. Para o cálculo do valor, foram realizados média e desvio padrão para padronização e criação de valores de referência, sendo utilizados -2DP para determinação do valor mínimo e + 2DP para o valor máximo. A enzima hepática ALT apresentou uma faixa de referência de 21 a 96 U / L, enquanto a AST de 44 a 186 U / L. Nos marcadores renais, a uréia foi de 20 a 73 mg / dL e a creatinina de 0,23 a 0,77 mg / dL. Dada a metodologia aplicada e os resultados obtidos, admite-se que é muito importante padronizar os valores de referência dos analitos em biotérios de animais devido a alterações geográficas, protocolos, dietas, entre outros, além disso, reduzindo o uso de animais em procedimentos experimentais, além de orientar pesquisadores sobre saúde animal e auxiliar a análise em vários projetos de pesquisa experimental.Research, Society and Development2020-03-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/261910.33448/rsd-v9i5.2619Research, Society and Development; Vol. 9 No. 5; e18952619Research, Society and Development; Vol. 9 Núm. 5; e18952619Research, Society and Development; v. 9 n. 5; e189526192525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/2619/5400Copyright (c) 2020 Silvio Almeida-Junior, Poliana Marques Pereira, Vanessa de Souza Totoli, Micaela Silva Carrijo, Fernanda Rafacho Badoco, Lucas de Freitas Pereira, Fernanda Gosuen Gonçalves Dias, Ricardo Andrade Furtadoinfo:eu-repo/semantics/openAccessAlmeida Junior, Silvio dePereira, Poliana MarquesTotoli, Vanessa de SouzaCarrijo, Micaela SilvaBadoco, Fernanda RafachoPereira, Lucas de FreitasDias, Fernanda Gosuen GonçalvesFurtado, Ricardo Andrade2020-08-20T18:06:36Zoai:ojs.pkp.sfu.ca:article/2619Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:27:09.246374Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Validation of renal and hepatic profile parameters of rodents Rattus novergicus kept in the maintenance vivarium of the University of Franca
Validación de los parámetros de perfil renal y hepático de roedores Rattus norvegicus mantenidos en el vivero de mantenimiento de la Universidad de Franca
Validação de parâmetros do perfil renal e hepático de roedores Rattus norvegicus do Biotério de manutenção da Universidade de Franca
title Validation of renal and hepatic profile parameters of rodents Rattus novergicus kept in the maintenance vivarium of the University of Franca
spellingShingle Validation of renal and hepatic profile parameters of rodents Rattus novergicus kept in the maintenance vivarium of the University of Franca
Almeida Junior, Silvio de
bioquímica clínica
biomarcadores
biotérios
enzimas séricas
ratos.
bioquímica clínica
biomarcadores
viveros
enzimas séricas
ratas
clinical biochemistry
biomarker
vivarium
serum enzymes
rats
title_short Validation of renal and hepatic profile parameters of rodents Rattus novergicus kept in the maintenance vivarium of the University of Franca
title_full Validation of renal and hepatic profile parameters of rodents Rattus novergicus kept in the maintenance vivarium of the University of Franca
title_fullStr Validation of renal and hepatic profile parameters of rodents Rattus novergicus kept in the maintenance vivarium of the University of Franca
title_full_unstemmed Validation of renal and hepatic profile parameters of rodents Rattus novergicus kept in the maintenance vivarium of the University of Franca
title_sort Validation of renal and hepatic profile parameters of rodents Rattus novergicus kept in the maintenance vivarium of the University of Franca
author Almeida Junior, Silvio de
author_facet Almeida Junior, Silvio de
Pereira, Poliana Marques
Totoli, Vanessa de Souza
Carrijo, Micaela Silva
Badoco, Fernanda Rafacho
Pereira, Lucas de Freitas
Dias, Fernanda Gosuen Gonçalves
Furtado, Ricardo Andrade
author_role author
author2 Pereira, Poliana Marques
Totoli, Vanessa de Souza
Carrijo, Micaela Silva
Badoco, Fernanda Rafacho
Pereira, Lucas de Freitas
Dias, Fernanda Gosuen Gonçalves
Furtado, Ricardo Andrade
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida Junior, Silvio de
Pereira, Poliana Marques
Totoli, Vanessa de Souza
Carrijo, Micaela Silva
Badoco, Fernanda Rafacho
Pereira, Lucas de Freitas
Dias, Fernanda Gosuen Gonçalves
Furtado, Ricardo Andrade
dc.subject.por.fl_str_mv bioquímica clínica
biomarcadores
biotérios
enzimas séricas
ratos.
bioquímica clínica
biomarcadores
viveros
enzimas séricas
ratas
clinical biochemistry
biomarker
vivarium
serum enzymes
rats
topic bioquímica clínica
biomarcadores
biotérios
enzimas séricas
ratos.
bioquímica clínica
biomarcadores
viveros
enzimas séricas
ratas
clinical biochemistry
biomarker
vivarium
serum enzymes
rats
description Due to the high use of animals as experimental models for pharmaceutical trials, especially rats, it is necessary to standardize reference values for the evaluation of liver perfil by the analytes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and renal function, through the analytes urea and creatinine. For this purpose, samples were collected from 169 samples of Rattus norvegicus of Wistar strain, from the Vivarium of the University of Franca (SP), provided of semi-automated analytical methodology. For the calculation of the value, mean and standard deviation were performed for standardization and for the creation of reference values, therefore -2DP for minimum value determination and + 2DP for maximum value were used. The liver enzyme ALT presented a reference range from 21 to 96 U / L, while the AST from 44 to 186 U / L. In renal markers, urea was 20 to 73 mg / dL and creatinine 0.23 to 0.77 mg / dL. Given the applied methodology and the results obtained, it is admitted that it is very important to standardize the reference values of analytes in animal houses due to geographical changes, protocols, diets, among others; In addition, reducing the use of animals in experimental procedures in addition to guiding researchers on animal health and assisting analysis in various experimental research projects.
publishDate 2020
dc.date.none.fl_str_mv 2020-03-26
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/2619
10.33448/rsd-v9i5.2619
url https://rsdjournal.org/index.php/rsd/article/view/2619
identifier_str_mv 10.33448/rsd-v9i5.2619
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/2619/5400
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 9 No. 5; e18952619
Research, Society and Development; Vol. 9 Núm. 5; e18952619
Research, Society and Development; v. 9 n. 5; e18952619
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052734344527872

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