Affinity of Staphylococcus aureus for prostheses colonization compared to other bacteria. An in vitro study
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/14701 |
Resumo: | Staphylococcus aureus biofilms have been recognized as a leading cause of multiple infections, including implant-associated infections and chronic wounds. We evaluated the colonization capacity of two distinct textured prostheses by different bacterial strains. Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Proteus mirabilis and Enterococcus faecalis were evaluated. Initially, the hydrophobicity and biofilm formation capacity were determined. Subsequently, 20 fragments of vascular prosthesis and 20 silicone prostheses were embedded in suspensions with the microorganisms and incubated. The prostheses were then sown in culture medium and incubated for 48 hours. Petri dishes were photographed and analyzed by fractal dimension. The Kruskal-Wallis test and the Dunn test were applied for the analysis of biofilm formation. To compare the mean intensity for the type of bacteria and the type of prosthesis, a general linear model was applied. Staphylococcus aureus was the bacterium with the highest colonization density in both prostheses (p = 0.0001). E. coli showed strong adherence in the biofilm formation capacity test (p = 0.0001), however, it did not colonize either prosthesis. We demonstrated that Staphylococcus aureus has a greater affinity for vascular and silicone prostheses than other bacteria. |
id |
UNIFEI_a2139616946649ab40073a4398b9395c |
---|---|
oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/14701 |
network_acronym_str |
UNIFEI |
network_name_str |
Research, Society and Development |
repository_id_str |
|
spelling |
Affinity of Staphylococcus aureus for prostheses colonization compared to other bacteria. An in vitro studyAfinidad de Staphylococcus aureus por la colonización de prótesis en comparación con otras bacterias. Un estudio in vitroAfinidade de Staphylococcus aureus para colonização de próteses em comparação com outras bactérias. Um estudo in vitroBiofilmesPróteses e implantesPolitetrafluoroetilenoProcedimentos cirúrgicos vascularesImplantes mamários.BiopelículasPrótesis e implantesPolitetrafluoroetilenoProcedimientos quirúrgicos vascularesImplante mamario.BiofilmsProstheses and implantsPolytetrafluoroethyleneVascular surgical proceduresBreast implant. Staphylococcus aureus biofilms have been recognized as a leading cause of multiple infections, including implant-associated infections and chronic wounds. We evaluated the colonization capacity of two distinct textured prostheses by different bacterial strains. Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Proteus mirabilis and Enterococcus faecalis were evaluated. Initially, the hydrophobicity and biofilm formation capacity were determined. Subsequently, 20 fragments of vascular prosthesis and 20 silicone prostheses were embedded in suspensions with the microorganisms and incubated. The prostheses were then sown in culture medium and incubated for 48 hours. Petri dishes were photographed and analyzed by fractal dimension. The Kruskal-Wallis test and the Dunn test were applied for the analysis of biofilm formation. To compare the mean intensity for the type of bacteria and the type of prosthesis, a general linear model was applied. Staphylococcus aureus was the bacterium with the highest colonization density in both prostheses (p = 0.0001). E. coli showed strong adherence in the biofilm formation capacity test (p = 0.0001), however, it did not colonize either prosthesis. We demonstrated that Staphylococcus aureus has a greater affinity for vascular and silicone prostheses than other bacteria.Se ha reconocido que las biopelículas de Staphylococcus aureus son una de las principales causas de infecciones múltiples, incluidas las infecciones asociadas a implantes y las heridas crónicas. Evaluamos la capacidad de colonización de dos prótesis texturizadas distintas por diferentes cepas bacterianas. Se evaluaron Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Proteus mirabilis y Enterococcus faecalis. Inicialmente se determinó la hidrofobicidad y la capacidad de formación de biopelículas. Posteriormente, se embebieron 20 fragmentos de prótesis vasculares y 20 prótesis de silicona en suspensiones con los microorganismos y se incubaron. A continuación, las prótesis se sembraron en medio de cultivo y se incubaron durante 48 horas. Las placas de Petri se fotografiaron y analizaron por dimensión fractal. Se aplicaron la prueba de Kruskal-Wallis y la prueba de Dunn para el análisis de la formación de biopelículas. Para comparar la intensidad media para el tipo de bacteria y el tipo de prótesis, se aplicó un modelo lineal general. Staphylococcus aureus fue la bacteria con mayor densidad de colonización en ambas prótesis (p = 0,0001). Escherichia coli mostró una fuerte adherencia en la prueba de capacidad de formación de biopelículas (p = 0,0001), sin embargo, no colonizó ninguna de las prótesis. Demostramos que Staphylococcus aureus tiene una mayor afinidad por las prótesis vasculares y de silicona que otras bacterias.Biofilmes de Staphylococcus aureus foram reconhecidos como uma das principais causas de infecções múltiplas, incluindo infecções associadas a implantes e feridas crônicas. Avaliamos a capacidade de colonização de duas próteses texturizadas distintas por diferentes cepas bacterianas. Foram avaliados Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Proteus mirabilis e Enterococcus faecalis. Inicialmente, foram determinadas a hidrofobicidade e a capacidade de formação de biofilme. Posteriormente, 20 fragmentos de próteses vasculares e 20 próteses de silicone foram incluídos em suspensões com os microrganismos e incubados. As próteses foram então semeadas em meio de cultura e incubadas por 48 horas. As placas de Petri foram fotografadas e analisadas pela dimensão fractal. O teste de Kruskal-Wallis e o teste de Dunn foram aplicados para a análise da formação de biofilme. Para comparar a intensidade média para o tipo de bactéria e o tipo de prótese, foi aplicado um modelo linear geral. Staphylococcus aureus foi a bactéria com maior densidade de colonização em ambas as próteses (p = 0,0001). Escherichia coli apresentou forte aderência no teste de capacidade de formação de biofilme (p = 0,0001), porém não colonizou nenhuma das próteses. Demonstramos que o Staphylococcus aureus tem maior afinidade por próteses vasculares e de silicone do que outras bactérias.Research, Society and Development2021-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1470110.33448/rsd-v10i5.14701Research, Society and Development; Vol. 10 No. 5; e15310514701Research, Society and Development; Vol. 10 Núm. 5; e15310514701Research, Society and Development; v. 10 n. 5; e153105147012525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/14701/13267Copyright (c) 2021 Gisele Alborghetti Nai; Denis Aloísio Lopes Medina; Cesar Alberto Talavera Martelli; Mayla Silva Cayres de Oliveira; Isadora Delfino Caldeira; Bruno Carvalho Henriques; Maria Júlia Schadeck Portelinha; Mércia de Carvalho Almeida; Lizziane Kretli Winkelstroter Eller; Fausto Viterbo de Oliveira Neto; Mariângela Esther Alencar Marqueshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessNai, Gisele AlborghettiMedina, Denis Aloísio Lopes Martelli, Cesar Alberto TalaveraOliveira, Mayla Silva Cayres de Caldeira, Isadora Delfino Henriques, Bruno CarvalhoPortelinha, Maria Júlia SchadeckAlmeida, Mércia de Carvalho Eller, Lizziane Kretli Winkelstroter Oliveira Neto, Fausto Viterbo de Marques, Mariângela Esther Alencar 2021-05-17T18:20:49Zoai:ojs.pkp.sfu.ca:article/14701Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:35:45.082546Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Affinity of Staphylococcus aureus for prostheses colonization compared to other bacteria. An in vitro study Afinidad de Staphylococcus aureus por la colonización de prótesis en comparación con otras bacterias. Un estudio in vitro Afinidade de Staphylococcus aureus para colonização de próteses em comparação com outras bactérias. Um estudo in vitro |
title |
Affinity of Staphylococcus aureus for prostheses colonization compared to other bacteria. An in vitro study |
spellingShingle |
Affinity of Staphylococcus aureus for prostheses colonization compared to other bacteria. An in vitro study Nai, Gisele Alborghetti Biofilmes Próteses e implantes Politetrafluoroetileno Procedimentos cirúrgicos vasculares Implantes mamários. Biopelículas Prótesis e implantes Politetrafluoroetileno Procedimientos quirúrgicos vasculares Implante mamario. Biofilms Prostheses and implants Polytetrafluoroethylene Vascular surgical procedures Breast implant. |
title_short |
Affinity of Staphylococcus aureus for prostheses colonization compared to other bacteria. An in vitro study |
title_full |
Affinity of Staphylococcus aureus for prostheses colonization compared to other bacteria. An in vitro study |
title_fullStr |
Affinity of Staphylococcus aureus for prostheses colonization compared to other bacteria. An in vitro study |
title_full_unstemmed |
Affinity of Staphylococcus aureus for prostheses colonization compared to other bacteria. An in vitro study |
title_sort |
Affinity of Staphylococcus aureus for prostheses colonization compared to other bacteria. An in vitro study |
author |
Nai, Gisele Alborghetti |
author_facet |
Nai, Gisele Alborghetti Medina, Denis Aloísio Lopes Martelli, Cesar Alberto Talavera Oliveira, Mayla Silva Cayres de Caldeira, Isadora Delfino Henriques, Bruno Carvalho Portelinha, Maria Júlia Schadeck Almeida, Mércia de Carvalho Eller, Lizziane Kretli Winkelstroter Oliveira Neto, Fausto Viterbo de Marques, Mariângela Esther Alencar |
author_role |
author |
author2 |
Medina, Denis Aloísio Lopes Martelli, Cesar Alberto Talavera Oliveira, Mayla Silva Cayres de Caldeira, Isadora Delfino Henriques, Bruno Carvalho Portelinha, Maria Júlia Schadeck Almeida, Mércia de Carvalho Eller, Lizziane Kretli Winkelstroter Oliveira Neto, Fausto Viterbo de Marques, Mariângela Esther Alencar |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Nai, Gisele Alborghetti Medina, Denis Aloísio Lopes Martelli, Cesar Alberto Talavera Oliveira, Mayla Silva Cayres de Caldeira, Isadora Delfino Henriques, Bruno Carvalho Portelinha, Maria Júlia Schadeck Almeida, Mércia de Carvalho Eller, Lizziane Kretli Winkelstroter Oliveira Neto, Fausto Viterbo de Marques, Mariângela Esther Alencar |
dc.subject.por.fl_str_mv |
Biofilmes Próteses e implantes Politetrafluoroetileno Procedimentos cirúrgicos vasculares Implantes mamários. Biopelículas Prótesis e implantes Politetrafluoroetileno Procedimientos quirúrgicos vasculares Implante mamario. Biofilms Prostheses and implants Polytetrafluoroethylene Vascular surgical procedures Breast implant. |
topic |
Biofilmes Próteses e implantes Politetrafluoroetileno Procedimentos cirúrgicos vasculares Implantes mamários. Biopelículas Prótesis e implantes Politetrafluoroetileno Procedimientos quirúrgicos vasculares Implante mamario. Biofilms Prostheses and implants Polytetrafluoroethylene Vascular surgical procedures Breast implant. |
description |
Staphylococcus aureus biofilms have been recognized as a leading cause of multiple infections, including implant-associated infections and chronic wounds. We evaluated the colonization capacity of two distinct textured prostheses by different bacterial strains. Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Proteus mirabilis and Enterococcus faecalis were evaluated. Initially, the hydrophobicity and biofilm formation capacity were determined. Subsequently, 20 fragments of vascular prosthesis and 20 silicone prostheses were embedded in suspensions with the microorganisms and incubated. The prostheses were then sown in culture medium and incubated for 48 hours. Petri dishes were photographed and analyzed by fractal dimension. The Kruskal-Wallis test and the Dunn test were applied for the analysis of biofilm formation. To compare the mean intensity for the type of bacteria and the type of prosthesis, a general linear model was applied. Staphylococcus aureus was the bacterium with the highest colonization density in both prostheses (p = 0.0001). E. coli showed strong adherence in the biofilm formation capacity test (p = 0.0001), however, it did not colonize either prosthesis. We demonstrated that Staphylococcus aureus has a greater affinity for vascular and silicone prostheses than other bacteria. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-05-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/14701 10.33448/rsd-v10i5.14701 |
url |
https://rsdjournal.org/index.php/rsd/article/view/14701 |
identifier_str_mv |
10.33448/rsd-v10i5.14701 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/14701/13267 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 5; e15310514701 Research, Society and Development; Vol. 10 Núm. 5; e15310514701 Research, Society and Development; v. 10 n. 5; e15310514701 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052748564267008 |