Protocols to germination and tetrazolium for physiological quality evaluation of Aspidosperma pyrifolium Mart.
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
DOI: | 10.33448/rsd-v10i9.18273 |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/18273 |
Resumo: | Standardized germination and tetrazolium test protocols are important for the analysis of seeds of Aspidosperma pyrifolium, a forest species of economic value and still poorly studied according to the literature, which is the objective of the present work. Thus, the following were tested: Germination (effect of substrates and temperatures) and Tetrazolium (effects of soaking and immersion times in concentrations of the tetrazolium solution). For the germination test, the paper roll and vermiculite substrates and temperatures of 25, 30 and 25-30ºC showed the best values (means between 65-90% germination). In the tetrazolium test, the treatment with 10 hours of soaking and 3 hours of immersion in the solution with a concentration of 0.075% (average of 90% viability) was the best. A positive correlation (Pearson) was found between the use of paper substrate at 25ºC and the abovementioned Tetrazolium treatment. Both described protocols [Germination (paper substrate at 25ºC for eight days) and Tetrazolium (10h of imbibition and 3h of immersion in the solution with a concentration of 0.075%)] can be tested in seeds of other populations of this species for their effectiveness. |
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Research, Society and Development |
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Protocols to germination and tetrazolium for physiological quality evaluation of Aspidosperma pyrifolium Mart. Protocolos de hermanamiento y tetrazolio para la evaluación de la calidad fisiológica de semillas de Aspidosperma pyrifolium Mart.Protocolos de germinação e tetrazólio para a avaliação da qualidade fisiológica de sementes de Aspidosperma pyrifolium Mart.CaatingaSementes florestaisVigorPereiro.CaatingaForest seedsVigorPereiro.CaatingaSementes florestaisVigorPereiro.Standardized germination and tetrazolium test protocols are important for the analysis of seeds of Aspidosperma pyrifolium, a forest species of economic value and still poorly studied according to the literature, which is the objective of the present work. Thus, the following were tested: Germination (effect of substrates and temperatures) and Tetrazolium (effects of soaking and immersion times in concentrations of the tetrazolium solution). For the germination test, the paper roll and vermiculite substrates and temperatures of 25, 30 and 25-30ºC showed the best values (means between 65-90% germination). In the tetrazolium test, the treatment with 10 hours of soaking and 3 hours of immersion in the solution with a concentration of 0.075% (average of 90% viability) was the best. A positive correlation (Pearson) was found between the use of paper substrate at 25ºC and the abovementioned Tetrazolium treatment. Both described protocols [Germination (paper substrate at 25ºC for eight days) and Tetrazolium (10h of imbibition and 3h of immersion in the solution with a concentration of 0.075%)] can be tested in seeds of other populations of this species for their effectiveness.Los protocolos estandarizados de germinación y ensayo de tetrazolio son importantes para el análisis de semillas de Aspidosperma pyrifolium, una especie forestal de valor económico y aún poco estudiada según la literatura, que es el objetivo del presente trabajo. Así, se ensayaron los siguientes: Germinación (efecto de sustratos y temperaturas) y Tetrazolio (efectos de los tiempos de remojo e inmersión en concentraciones de la solución de tetrazolio). Para la prueba de germinación, los sustratos de rollo de papel y vermiculita y temperaturas de 25, 30 y 25-30ºC mostraron los mejores valores (medias entre 65-90% de germinación). En la prueba de tetrazolio, el tratamiento con 10 h de remojo y 3 h de inmersión en la solución con una concentración de 0.075% (promedio de 90% de viabilidad) fue el mejor. Se encontró una correlación positiva (Pearson) entre el uso de sustrato de papel a 25ºC y el tratamiento con tetrazolio antes mencionado. Ambos protocolos descritos [Germinación (sustrato de papel a 25ºC durante ocho días) y Tetrazolio (10h de imbibición y 3h de inmersión en la solución con una concentración de 0,075%)] pueden ensayarse en semillas de otras poblaciones de esta especie para su eficacia.Protocolos de testes de germinação e tetrazólio padronizados são importantes para a análise de sementes, inclusive para a espécie Aspidosperma pyrifolium, esseencia florestal de valor econômico e ainda pouco estudada segundo a literatura, sendo, portanto, este o objetivo do presente trabalho. Assim, foram testados protocolos de Germinação (efeito de substratos e temperaturas) e Tetrazólio (efeitos de tempos de embebição e de imersão em concentrações da solução de tetrazólio). Para o teste de Germinação, os substratos rolo de papel e vermiculita e as temperaturas de 25, 30 e 25-30ºC apresentaram os melhores valores (médias entre 65-90% de germinação). No teste de tetrazólio o tratamento com 10h de embebição e 3h de imersão na solução com concentração de 0,075% (média de 90% de viabilidade) foi o melhor. Foi constatada correlação positiva (Pearson) entre a utilização do substrato papel a 25ºC e o referido tratamento de Tetrazólio. Ambos protocolos descritos [Germinação (substrato papel a 25ºC por oito dias) e Tetrazolio (10h de embebição e 3h de imersão na solução com concentração de 0,075%)] podem testados em sementes de outras populações desta espécie para a verificação da efetividade deste teste.Research, Society and Development2021-08-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1827310.33448/rsd-v10i9.18273Research, Society and Development; Vol. 10 No. 9; e57910918273Research, Society and Development; Vol. 10 Núm. 9; e57910918273Research, Society and Development; v. 10 n. 9; e579109182732525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/18273/16490Copyright (c) 2021 Maria do Carmo Learth Cunha; Josueldo Alves Lopes; Anderlon Arrais de Moraes Monte; Thiago Costa Ferreira; Manoel Rivelino Gomes de Oliveirahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCunha, Maria do Carmo Learth Lopes, Josueldo Alves Monte, Anderlon Arrais de Moraes Ferreira, Thiago CostaOliveira, Manoel Rivelino Gomes de 2021-09-12T14:28:06Zoai:ojs.pkp.sfu.ca:article/18273Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:38:26.199972Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Protocols to germination and tetrazolium for physiological quality evaluation of Aspidosperma pyrifolium Mart. Protocolos de hermanamiento y tetrazolio para la evaluación de la calidad fisiológica de semillas de Aspidosperma pyrifolium Mart. Protocolos de germinação e tetrazólio para a avaliação da qualidade fisiológica de sementes de Aspidosperma pyrifolium Mart. |
title |
Protocols to germination and tetrazolium for physiological quality evaluation of Aspidosperma pyrifolium Mart. |
spellingShingle |
Protocols to germination and tetrazolium for physiological quality evaluation of Aspidosperma pyrifolium Mart. Protocols to germination and tetrazolium for physiological quality evaluation of Aspidosperma pyrifolium Mart. Cunha, Maria do Carmo Learth Caatinga Sementes florestais Vigor Pereiro. Caatinga Forest seeds Vigor Pereiro. Caatinga Sementes florestais Vigor Pereiro. Cunha, Maria do Carmo Learth Caatinga Sementes florestais Vigor Pereiro. Caatinga Forest seeds Vigor Pereiro. Caatinga Sementes florestais Vigor Pereiro. |
title_short |
Protocols to germination and tetrazolium for physiological quality evaluation of Aspidosperma pyrifolium Mart. |
title_full |
Protocols to germination and tetrazolium for physiological quality evaluation of Aspidosperma pyrifolium Mart. |
title_fullStr |
Protocols to germination and tetrazolium for physiological quality evaluation of Aspidosperma pyrifolium Mart. Protocols to germination and tetrazolium for physiological quality evaluation of Aspidosperma pyrifolium Mart. |
title_full_unstemmed |
Protocols to germination and tetrazolium for physiological quality evaluation of Aspidosperma pyrifolium Mart. Protocols to germination and tetrazolium for physiological quality evaluation of Aspidosperma pyrifolium Mart. |
title_sort |
Protocols to germination and tetrazolium for physiological quality evaluation of Aspidosperma pyrifolium Mart. |
author |
Cunha, Maria do Carmo Learth |
author_facet |
Cunha, Maria do Carmo Learth Cunha, Maria do Carmo Learth Lopes, Josueldo Alves Monte, Anderlon Arrais de Moraes Ferreira, Thiago Costa Oliveira, Manoel Rivelino Gomes de Lopes, Josueldo Alves Monte, Anderlon Arrais de Moraes Ferreira, Thiago Costa Oliveira, Manoel Rivelino Gomes de |
author_role |
author |
author2 |
Lopes, Josueldo Alves Monte, Anderlon Arrais de Moraes Ferreira, Thiago Costa Oliveira, Manoel Rivelino Gomes de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Cunha, Maria do Carmo Learth Lopes, Josueldo Alves Monte, Anderlon Arrais de Moraes Ferreira, Thiago Costa Oliveira, Manoel Rivelino Gomes de |
dc.subject.por.fl_str_mv |
Caatinga Sementes florestais Vigor Pereiro. Caatinga Forest seeds Vigor Pereiro. Caatinga Sementes florestais Vigor Pereiro. |
topic |
Caatinga Sementes florestais Vigor Pereiro. Caatinga Forest seeds Vigor Pereiro. Caatinga Sementes florestais Vigor Pereiro. |
description |
Standardized germination and tetrazolium test protocols are important for the analysis of seeds of Aspidosperma pyrifolium, a forest species of economic value and still poorly studied according to the literature, which is the objective of the present work. Thus, the following were tested: Germination (effect of substrates and temperatures) and Tetrazolium (effects of soaking and immersion times in concentrations of the tetrazolium solution). For the germination test, the paper roll and vermiculite substrates and temperatures of 25, 30 and 25-30ºC showed the best values (means between 65-90% germination). In the tetrazolium test, the treatment with 10 hours of soaking and 3 hours of immersion in the solution with a concentration of 0.075% (average of 90% viability) was the best. A positive correlation (Pearson) was found between the use of paper substrate at 25ºC and the abovementioned Tetrazolium treatment. Both described protocols [Germination (paper substrate at 25ºC for eight days) and Tetrazolium (10h of imbibition and 3h of immersion in the solution with a concentration of 0.075%)] can be tested in seeds of other populations of this species for their effectiveness. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-03 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/18273 10.33448/rsd-v10i9.18273 |
url |
https://rsdjournal.org/index.php/rsd/article/view/18273 |
identifier_str_mv |
10.33448/rsd-v10i9.18273 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/18273/16490 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 9; e57910918273 Research, Society and Development; Vol. 10 Núm. 9; e57910918273 Research, Society and Development; v. 10 n. 9; e57910918273 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1822178548172980224 |
dc.identifier.doi.none.fl_str_mv |
10.33448/rsd-v10i9.18273 |