Anti-leishmania activity of extract and fractions from the stem and leaf of Montrichardia linifera (Arruda) schott (Araceae) against Leishmania amazonensis
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/12312 |
Resumo: | This study aimed to evaluate the anti-leishmania activity of extracts and fractions from stem and leaf of Montrichardia linifera against Leishmania amazonensis. The stem (EES) and leaf (EEL) extracts were obtained by maceration of powders with 96º GL alcohol. The extracts were subjected to exhaustive extraction using a reflux system and solvents of increasing polarity, obtaining 4 fractions for each extract: hexane, dichloromethane, ethyl acetate and methanol. The extracts and fractions were submitted to a phytochemical prospecting study. The antipromastigote activity and cytotoxicity in macrophages (J774) were performed using the cell viability test (MTT). In the extract and fractions of the stem, alkaloids, steroids, terpenes, flavonic heterosides, tannins, polyphenols and saponins were detected. In the extract and fractions of the leaves, coumarins, steroids, terpenes, flavonic heterosides, tannins, polyphenols and saponins were detected. In the anti-leishmania evaluation, the hexane fraction of the leaf (HFL) showed promising activity (IC50=38.56 µg/mL), and the hexane fraction of the stem (HFS) showed moderate activity (CI50=179.3 µg/mL), the extracts and the other fractions were inactive (IC50>200 µg/mL). In the cytotoxicity test, EES and HFS were cytotoxic (CC50 54.82 µg/mL and 26.95 µg/mL, respectively). EEF and HFL showed moderate cytotoxicity (CC50 of 162.7 µg/mL and 347.1 µg/mL). As for the selectivity index, the HFL showed high selectivity (SI=90). In summary, fractionation contributed to increase anti-leishmania activity and the selectivity of HFS, such activity may be related to steroids or terpenes. |
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Anti-leishmania activity of extract and fractions from the stem and leaf of Montrichardia linifera (Arruda) schott (Araceae) against Leishmania amazonensisActividad antileishmania del extracto y fracciones del tallo y hoja de Montrichardia linifera (Arruda) schott (Araceae) contra Leishmania amazonensisAtividade antileishmania do extrato e frações do caule e folha de Montrichardia linifera (Arruda) schott (Araceae) contra Leishmania amazonensis Anti-leishmaniaAningaTerpenesSaponins.Anti-leishmaniaAningaTerpenosSaponinas.Anti-leishmaniaAningaTerpenosSaponinas.This study aimed to evaluate the anti-leishmania activity of extracts and fractions from stem and leaf of Montrichardia linifera against Leishmania amazonensis. The stem (EES) and leaf (EEL) extracts were obtained by maceration of powders with 96º GL alcohol. The extracts were subjected to exhaustive extraction using a reflux system and solvents of increasing polarity, obtaining 4 fractions for each extract: hexane, dichloromethane, ethyl acetate and methanol. The extracts and fractions were submitted to a phytochemical prospecting study. The antipromastigote activity and cytotoxicity in macrophages (J774) were performed using the cell viability test (MTT). In the extract and fractions of the stem, alkaloids, steroids, terpenes, flavonic heterosides, tannins, polyphenols and saponins were detected. In the extract and fractions of the leaves, coumarins, steroids, terpenes, flavonic heterosides, tannins, polyphenols and saponins were detected. In the anti-leishmania evaluation, the hexane fraction of the leaf (HFL) showed promising activity (IC50=38.56 µg/mL), and the hexane fraction of the stem (HFS) showed moderate activity (CI50=179.3 µg/mL), the extracts and the other fractions were inactive (IC50>200 µg/mL). In the cytotoxicity test, EES and HFS were cytotoxic (CC50 54.82 µg/mL and 26.95 µg/mL, respectively). EEF and HFL showed moderate cytotoxicity (CC50 of 162.7 µg/mL and 347.1 µg/mL). As for the selectivity index, the HFL showed high selectivity (SI=90). In summary, fractionation contributed to increase anti-leishmania activity and the selectivity of HFS, such activity may be related to steroids or terpenes.Este estudio tuvo como objetivo evaluar la actividad antileishmania de los extractos y fracciones del tallo y hoja de Montrichardia linifera contra Leishmania amazonensis. Los extractos de tallo (EEC) y hoja (EEF) se obtuvieron de la maceración de los polvos con alcohol de 96º GL. Los extractos se sometieron a una extracción exhaustiva mediante un sistema de reflujo y disolventes de polaridad creciente, obteniendo 4 fracciones por cada extracto: hexano, diclorometano, acetato de etilo y metanol. Los extractos y fracciones se sometieron a un estudio de prospección fitoquímica. La actividad antipromastigota y la citotoxicidad en macrófagos (J774) se realizaron mediante la prueba de viabilidad celular (MTT). En el extracto y fracciones de tallo se detectaron alcaloides, esteroides, terpenos, heterósidos flavónicos, taninos, polifenoles y saponinas. En el extracto y fracciones de las hojas se detectaron cumarinas, esteroides, terpenos, heterósidos flavónicos, taninos, polifenoles y saponinas. En la evaluación de antileishmania, la fracción de hexano foliar (FHF) mostró actividad prometedora (IC50=38.56 µg/mL) y la fracción de hexano de tallo (FHC) mostró actividad moderada (CI50=179.3 µg/mL), los extractos y las otras fracciones estaban inactivos (IC50>200 µg/mL). En la prueba de citotoxicidad, EEC y FHC fueron citotóxicos (CC50 de 54.82 µg/mL y 26.95 µg/mL, respectivamente). EEF y FHF mostraron citotoxicidad moderada (CC50 de 162,7 µg/mL y 347,1 µg/mL). En cuanto al índice de selectividad, la fracción FHF mostró una alta selectividad (IS=90). En resumen, el fraccionamiento contribuyó al aumento de la actividad antileishmania y la selectividad de FHF, tal actividad puede estar relacionada con esteroides o terpenos.Este estudo teve como objetivo avaliar a atividade antileishmania dos extratos e frações do caule e da folha de Montrichardia linifera contra Leishmania amazonensis. Os extratos do caule (EEC) e da folha (EEF) foram obtidos a partir da maceração dos pós com álcool 96º GL. Os extratos foram submetidos à extração exaustiva utilizando um sistema de refluxo e solventes de polaridades crescentes, obtendo-se 4 frações para cada extrato: hexano, diclorometano, acetato de etila e metanol. Os extratos e frações foram submetidos ao estudo de prospecção fitoquímica. A atividade antipromastigota e a citotoxicidade em macrófagos (J774) foram realizados através do teste de viabilidade celular (MTT). No extrato e nas frações do caule, foram detectados alcaloides, esteroides, terpenos, heterosídeos flavônicos, taninos, polifenóis e saponinas. No extrato e frações das folhas, foram detectados cumarinas, esteroides, terpenos, heterosídeos flavônicos, taninos, polifenois e saponinas. Na avaliação antileishmania, a fração de hexano das folhas (FHF) apresentou promissora atividade (CI50=38,56 µg/mL), e a fração de hexano do caule (FHC) apresentou moderada atividade (CI50=179,3 µg/mL), os extratos e as demais frações foram inativos (CI50>200 µg/mL). No teste de citotoxicidade, o EEC e a FHC foram citotóxicos (CC50 de 54,82 µg/mL e 26,95 µg/mL, respectivamente). O EEF e a FHF apresentaram moderada citotoxicidade (CC50 de 162,7 µg/mL e 347,1 µg/mL). Quanto ao índice de seletividade, a fração FHF apresentou alta seletividade (IS=90). Em síntese, o fracionamento contribuíu para o aumento da atividade antileishmania e da seletividade da FHF, tal atividade pode estar relacionada com esteroides ou terpenos.Research, Society and Development2021-02-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1231210.33448/rsd-v10i2.12312Research, Society and Development; Vol. 10 No. 2; e9310212312Research, Society and Development; Vol. 10 Núm. 2; e9310212312Research, Society and Development; v. 10 n. 2; e93102123122525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/12312/11015Copyright (c) 2021 Lara Vinha Faria; Heliton Patrick Cordovil Brígido; Alexandre Augusto Bentaberry-Rosa; Juliana Correa-Barbosa; João Victor Silva-Silva; Mirian Letícia Carmo Bastos; Erica Vanessa Souza Costa; Márlia Coelho-Ferreira; Fernando Tobias Silveira; Maria Fâni Dolabelahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFaria, Lara Vinha Brígido, Heliton Patrick Cordovil Bentaberry-Rosa, Alexandre Augusto Correa-Barbosa, JulianaSilva-Silva, João Victor Bastos, Mirian Letícia Carmo Costa, Erica Vanessa Souza Coelho-Ferreira, MárliaSilveira, Fernando Tobias Dolabela, Maria Fâni 2021-03-02T09:32:39Zoai:ojs.pkp.sfu.ca:article/12312Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:33:55.477417Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Anti-leishmania activity of extract and fractions from the stem and leaf of Montrichardia linifera (Arruda) schott (Araceae) against Leishmania amazonensis Actividad antileishmania del extracto y fracciones del tallo y hoja de Montrichardia linifera (Arruda) schott (Araceae) contra Leishmania amazonensis Atividade antileishmania do extrato e frações do caule e folha de Montrichardia linifera (Arruda) schott (Araceae) contra Leishmania amazonensis |
title |
Anti-leishmania activity of extract and fractions from the stem and leaf of Montrichardia linifera (Arruda) schott (Araceae) against Leishmania amazonensis |
spellingShingle |
Anti-leishmania activity of extract and fractions from the stem and leaf of Montrichardia linifera (Arruda) schott (Araceae) against Leishmania amazonensis Faria, Lara Vinha Anti-leishmania Aninga Terpenes Saponins. Anti-leishmania Aninga Terpenos Saponinas. Anti-leishmania Aninga Terpenos Saponinas. |
title_short |
Anti-leishmania activity of extract and fractions from the stem and leaf of Montrichardia linifera (Arruda) schott (Araceae) against Leishmania amazonensis |
title_full |
Anti-leishmania activity of extract and fractions from the stem and leaf of Montrichardia linifera (Arruda) schott (Araceae) against Leishmania amazonensis |
title_fullStr |
Anti-leishmania activity of extract and fractions from the stem and leaf of Montrichardia linifera (Arruda) schott (Araceae) against Leishmania amazonensis |
title_full_unstemmed |
Anti-leishmania activity of extract and fractions from the stem and leaf of Montrichardia linifera (Arruda) schott (Araceae) against Leishmania amazonensis |
title_sort |
Anti-leishmania activity of extract and fractions from the stem and leaf of Montrichardia linifera (Arruda) schott (Araceae) against Leishmania amazonensis |
author |
Faria, Lara Vinha |
author_facet |
Faria, Lara Vinha Brígido, Heliton Patrick Cordovil Bentaberry-Rosa, Alexandre Augusto Correa-Barbosa, Juliana Silva-Silva, João Victor Bastos, Mirian Letícia Carmo Costa, Erica Vanessa Souza Coelho-Ferreira, Márlia Silveira, Fernando Tobias Dolabela, Maria Fâni |
author_role |
author |
author2 |
Brígido, Heliton Patrick Cordovil Bentaberry-Rosa, Alexandre Augusto Correa-Barbosa, Juliana Silva-Silva, João Victor Bastos, Mirian Letícia Carmo Costa, Erica Vanessa Souza Coelho-Ferreira, Márlia Silveira, Fernando Tobias Dolabela, Maria Fâni |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Faria, Lara Vinha Brígido, Heliton Patrick Cordovil Bentaberry-Rosa, Alexandre Augusto Correa-Barbosa, Juliana Silva-Silva, João Victor Bastos, Mirian Letícia Carmo Costa, Erica Vanessa Souza Coelho-Ferreira, Márlia Silveira, Fernando Tobias Dolabela, Maria Fâni |
dc.subject.por.fl_str_mv |
Anti-leishmania Aninga Terpenes Saponins. Anti-leishmania Aninga Terpenos Saponinas. Anti-leishmania Aninga Terpenos Saponinas. |
topic |
Anti-leishmania Aninga Terpenes Saponins. Anti-leishmania Aninga Terpenos Saponinas. Anti-leishmania Aninga Terpenos Saponinas. |
description |
This study aimed to evaluate the anti-leishmania activity of extracts and fractions from stem and leaf of Montrichardia linifera against Leishmania amazonensis. The stem (EES) and leaf (EEL) extracts were obtained by maceration of powders with 96º GL alcohol. The extracts were subjected to exhaustive extraction using a reflux system and solvents of increasing polarity, obtaining 4 fractions for each extract: hexane, dichloromethane, ethyl acetate and methanol. The extracts and fractions were submitted to a phytochemical prospecting study. The antipromastigote activity and cytotoxicity in macrophages (J774) were performed using the cell viability test (MTT). In the extract and fractions of the stem, alkaloids, steroids, terpenes, flavonic heterosides, tannins, polyphenols and saponins were detected. In the extract and fractions of the leaves, coumarins, steroids, terpenes, flavonic heterosides, tannins, polyphenols and saponins were detected. In the anti-leishmania evaluation, the hexane fraction of the leaf (HFL) showed promising activity (IC50=38.56 µg/mL), and the hexane fraction of the stem (HFS) showed moderate activity (CI50=179.3 µg/mL), the extracts and the other fractions were inactive (IC50>200 µg/mL). In the cytotoxicity test, EES and HFS were cytotoxic (CC50 54.82 µg/mL and 26.95 µg/mL, respectively). EEF and HFL showed moderate cytotoxicity (CC50 of 162.7 µg/mL and 347.1 µg/mL). As for the selectivity index, the HFL showed high selectivity (SI=90). In summary, fractionation contributed to increase anti-leishmania activity and the selectivity of HFS, such activity may be related to steroids or terpenes. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-02-06 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/12312 10.33448/rsd-v10i2.12312 |
url |
https://rsdjournal.org/index.php/rsd/article/view/12312 |
identifier_str_mv |
10.33448/rsd-v10i2.12312 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/12312/11015 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 2; e9310212312 Research, Society and Development; Vol. 10 Núm. 2; e9310212312 Research, Society and Development; v. 10 n. 2; e9310212312 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052745502425088 |