Biodegradation of diesel oil by Penicillium citrinum UCP 1183 isolated from mangrove sediments

Detalhes bibliográficos
Autor(a) principal: Costa, Everton Ricardo Carneiro
Data de Publicação: 2022
Outros Autores: Souza, Adriana Ferreira de, Rodríguez , Dayana Montero, Mendonça , Rafael de Souza, Silva, Allem Karolyne Dino da, Takaki, Galba Maria de Campos, Andrade, Rosileide Fontenele da Silva
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/33071
Resumo: Biodegradation is a very effective technology that can be used to recover areas impacted with petroderivatives. In this context, the aim of this work was to investigate the potential of Penicillium sp. (isolated from mangrove sediments in the state of Pernambuco) in the biodegradation of diesel oil. Identification of specie was performed by analyzing the macromorphological and micromorphological structures. The biodegradation study was carried out in Bushnell Haas medium containing the redox indicator 2,6-dichlorophenol indophenol (DCPIP) and the fungus previously acclimated to different concentrations of burnt motor oil. The assay was incubated under static conditions and darkness for 72 h and every 24 h the biodegradation of the petroderivative was verified by UV-Vis spectrophotometry at 600 nm. The characteristics of the fungus indicated that the isolate is Penicillium citrinum. In the acclimation stage, P. citrinum grew in all tested concentrations of burnt motor oil (5, 10, 15 and 20%). In addition, the fungus was able to degrade diesel oil by the proven oxidation-reduction reaction indicated by the change in the color of the indicator from blue (oxidized form) to colorless (reduced form). The spectrophotometric analysis verified the occurrence of the biodegradation of diesel oil, resulting in the maximum value (70%) after 72 h. The present work demonstrates that the mangrove sediment isolate was identified as P. citrinum and it has promising potential for use in diesel oil biodegradation processes in impacted areas.
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spelling Biodegradation of diesel oil by Penicillium citrinum UCP 1183 isolated from mangrove sedimentsBiodegradación de diesel por Penicillium citrinum UCP 1183 aislado de sedimentos de manglarBiodegradação de óleo diesel por Penicillium citrinum UCP 1183 isolado de sedimentos de mangueFungo filamentosoBiorremediaçãoPetroderivadoDCPIP.Hongo filamentosoBiorremediaciónPetroderivadoDCPIP.Filamentous fungusBioremediationPetroderivativeDCPIP.Biodegradation is a very effective technology that can be used to recover areas impacted with petroderivatives. In this context, the aim of this work was to investigate the potential of Penicillium sp. (isolated from mangrove sediments in the state of Pernambuco) in the biodegradation of diesel oil. Identification of specie was performed by analyzing the macromorphological and micromorphological structures. The biodegradation study was carried out in Bushnell Haas medium containing the redox indicator 2,6-dichlorophenol indophenol (DCPIP) and the fungus previously acclimated to different concentrations of burnt motor oil. The assay was incubated under static conditions and darkness for 72 h and every 24 h the biodegradation of the petroderivative was verified by UV-Vis spectrophotometry at 600 nm. The characteristics of the fungus indicated that the isolate is Penicillium citrinum. In the acclimation stage, P. citrinum grew in all tested concentrations of burnt motor oil (5, 10, 15 and 20%). In addition, the fungus was able to degrade diesel oil by the proven oxidation-reduction reaction indicated by the change in the color of the indicator from blue (oxidized form) to colorless (reduced form). The spectrophotometric analysis verified the occurrence of the biodegradation of diesel oil, resulting in the maximum value (70%) after 72 h. The present work demonstrates that the mangrove sediment isolate was identified as P. citrinum and it has promising potential for use in diesel oil biodegradation processes in impacted areas.La biodegradación es una tecnología muy efectiva que se puede utilizar para recuperar áreas impactadas con petroderivados. En este contexto, el objetivo de este trabajo fue investigar el potencial de Penicillium sp. (aislado de sedimentos de manglar en el estado de Pernambuco) en la biodegradación de diesel. La identificación de especies se realizó analizando las estructuras macromorfológicas y micromorfológicas. El estudio de biodegradación se realizó en medio Bushnell Haas que contenía el indicador redox 2,6-diclorofenolindofenol (DCPIP) y el hongo previamente aclimatado a diferentes concentraciones de aceite de motor quemado. El ensayo se incubó en condiciones estáticas y en oscuridad durante 72 h y cada 24 h se verificó la biodegradación del petroderivado por espectrofotometría UV-Vis a 600 nm. Las características del hongo indicaron que el aislado es Penicillium citrinum. En la etapa de aclimatación, P. citrinum creció en todas las concentraciones de aceite de motor quemado (5, 10, 15 y 20%). Además, el hongo fue capaz de degradar diesel por la comprobada reacción de oxidación-reducción indicada por el cambio en el color del indicador azul (forma oxidada) a incoloro (forma reducida). El análisis espectrofotométrico verificó la ocurrencia de biodegradación de diesel, resultando en el valor máximo (70%) después de 72 h. El presente trabajo demuestra que el aislado de sedimento de manglar fue identificado como P. citrinum y tiene un potencial prometedor para su uso en procesos de biodegradación de diesel en áreas impactadas.A biodegradação é uma tecnologia bastante eficaz que pode ser utilizada para recuperação de áreas impactadas com petroderivados. Neste contexto, o objetivo deste trabalho foi investigar o potencial de Penicillium sp. (isolado de sedimentos de mangue no estado de Pernambuco) na biodegradação de óleo diesel. A identificação da espécie foi realizada pela análise das estruturas macromorfológica e micromorfológica. O estudo de biodegradação foi realizado em meio Bushnell Haas contendo o indicador redox 2,6- diclorofenol indofenol (DCPIP) e o fungo previamente aclimatado a diferentes concentrações do óleo de motor queimado. O ensaio foi incubado em condições estáticas e na ausência de luz durante 72h e a cada 24 h foi verificada a biodegradação do petroderivado por espectrofotometria UV-Vis a 600 nm. As características do fungo indicaram que o isolado é Penicillium citrinum. Na etapa de aclimatação, P. citrinum cresceu em todas as concentrações testadas do óleo de motor queimado (5, 10, 15 e 20%). Em adição, o fungo foi capaz de degradar o óleo diesel pela comprovada reação de oxi-redução indicada pela alteração da cor do indicador azul (forma oxidada) para incolor (forma reduzida). A análise espectrofotométrica verificou a ocorrência da biodegradação do óleo diesel, resultando no valor máximo (70%) após 72 h. O presente trabalho demonstra que o isolado de sedimentos de mangue foi identificado como P. citrinum e possui promissor potencial de uso em processos de biodegradação do óleo diesel em áreas impactadas.Research, Society and Development2022-08-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3307110.33448/rsd-v11i10.33071Research, Society and Development; Vol. 11 No. 10; e573111033071Research, Society and Development; Vol. 11 Núm. 10; e573111033071Research, Society and Development; v. 11 n. 10; e5731110330712525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/33071/28095Copyright (c) 2022 Everton Ricardo Carneiro Costa; Adriana Ferreira de Souza; Dayana Montero Rodríguez ; Rafael de Souza Mendonça ; Allem Karolyne Dino da Silva; Galba Maria de Campos Takaki; Rosileide Fontenele da Silva Andradehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCosta, Everton Ricardo Carneiro Souza, Adriana Ferreira deRodríguez , Dayana Montero Mendonça , Rafael de Souza Silva, Allem Karolyne Dino da Takaki, Galba Maria de Campos Andrade, Rosileide Fontenele da Silva 2022-08-12T22:23:03Zoai:ojs.pkp.sfu.ca:article/33071Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:48:50.491206Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Biodegradation of diesel oil by Penicillium citrinum UCP 1183 isolated from mangrove sediments
Biodegradación de diesel por Penicillium citrinum UCP 1183 aislado de sedimentos de manglar
Biodegradação de óleo diesel por Penicillium citrinum UCP 1183 isolado de sedimentos de mangue
title Biodegradation of diesel oil by Penicillium citrinum UCP 1183 isolated from mangrove sediments
spellingShingle Biodegradation of diesel oil by Penicillium citrinum UCP 1183 isolated from mangrove sediments
Costa, Everton Ricardo Carneiro
Fungo filamentoso
Biorremediação
Petroderivado
DCPIP.
Hongo filamentoso
Biorremediación
Petroderivado
DCPIP.
Filamentous fungus
Bioremediation
Petroderivative
DCPIP.
title_short Biodegradation of diesel oil by Penicillium citrinum UCP 1183 isolated from mangrove sediments
title_full Biodegradation of diesel oil by Penicillium citrinum UCP 1183 isolated from mangrove sediments
title_fullStr Biodegradation of diesel oil by Penicillium citrinum UCP 1183 isolated from mangrove sediments
title_full_unstemmed Biodegradation of diesel oil by Penicillium citrinum UCP 1183 isolated from mangrove sediments
title_sort Biodegradation of diesel oil by Penicillium citrinum UCP 1183 isolated from mangrove sediments
author Costa, Everton Ricardo Carneiro
author_facet Costa, Everton Ricardo Carneiro
Souza, Adriana Ferreira de
Rodríguez , Dayana Montero
Mendonça , Rafael de Souza
Silva, Allem Karolyne Dino da
Takaki, Galba Maria de Campos
Andrade, Rosileide Fontenele da Silva
author_role author
author2 Souza, Adriana Ferreira de
Rodríguez , Dayana Montero
Mendonça , Rafael de Souza
Silva, Allem Karolyne Dino da
Takaki, Galba Maria de Campos
Andrade, Rosileide Fontenele da Silva
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Everton Ricardo Carneiro
Souza, Adriana Ferreira de
Rodríguez , Dayana Montero
Mendonça , Rafael de Souza
Silva, Allem Karolyne Dino da
Takaki, Galba Maria de Campos
Andrade, Rosileide Fontenele da Silva
dc.subject.por.fl_str_mv Fungo filamentoso
Biorremediação
Petroderivado
DCPIP.
Hongo filamentoso
Biorremediación
Petroderivado
DCPIP.
Filamentous fungus
Bioremediation
Petroderivative
DCPIP.
topic Fungo filamentoso
Biorremediação
Petroderivado
DCPIP.
Hongo filamentoso
Biorremediación
Petroderivado
DCPIP.
Filamentous fungus
Bioremediation
Petroderivative
DCPIP.
description Biodegradation is a very effective technology that can be used to recover areas impacted with petroderivatives. In this context, the aim of this work was to investigate the potential of Penicillium sp. (isolated from mangrove sediments in the state of Pernambuco) in the biodegradation of diesel oil. Identification of specie was performed by analyzing the macromorphological and micromorphological structures. The biodegradation study was carried out in Bushnell Haas medium containing the redox indicator 2,6-dichlorophenol indophenol (DCPIP) and the fungus previously acclimated to different concentrations of burnt motor oil. The assay was incubated under static conditions and darkness for 72 h and every 24 h the biodegradation of the petroderivative was verified by UV-Vis spectrophotometry at 600 nm. The characteristics of the fungus indicated that the isolate is Penicillium citrinum. In the acclimation stage, P. citrinum grew in all tested concentrations of burnt motor oil (5, 10, 15 and 20%). In addition, the fungus was able to degrade diesel oil by the proven oxidation-reduction reaction indicated by the change in the color of the indicator from blue (oxidized form) to colorless (reduced form). The spectrophotometric analysis verified the occurrence of the biodegradation of diesel oil, resulting in the maximum value (70%) after 72 h. The present work demonstrates that the mangrove sediment isolate was identified as P. citrinum and it has promising potential for use in diesel oil biodegradation processes in impacted areas.
publishDate 2022
dc.date.none.fl_str_mv 2022-08-11
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/33071
10.33448/rsd-v11i10.33071
url https://rsdjournal.org/index.php/rsd/article/view/33071
identifier_str_mv 10.33448/rsd-v11i10.33071
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/33071/28095
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 10; e573111033071
Research, Society and Development; Vol. 11 Núm. 10; e573111033071
Research, Society and Development; v. 11 n. 10; e573111033071
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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