Onion (Allium cepa) DNA extraction: Perspective of scientific practices through laboratory practice

Detalhes bibliográficos
Autor(a) principal: Siqueira, Edmilson Clarindo de
Data de Publicação: 2023
Outros Autores: Doboszewski, Bogdan
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/44583
Resumo: DNA represents the structural unit of life, being one of the best-known organic substances. DNA extraction has become a alternative to teaching-learning in Biology classes. In this sense, the objective of this work was to facilitate the learning of high school Biology students, based on an experimental activity involving the extraction of DNA from onions (Allium cepa). The activity was implemented in a practical class laboratory at IFPE-Campus Barreiros in two pedagogical moments. The first moment consisted of a practical class for extracting DNA from A. cepa, using everyday materials. In the second moment, students were invited to evaluate the activity through a questionnaire. From the analysis of the results, it was possible to verify that the proposed activity encouraged motivation, aroused curiosity and facilitated students learning. Furthermore, the evaluation of the activity by the students was considered stimulating by the authors. Finally, in addition to combining theory with practice, this work can be useful as reference material on the DNA molecule.
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spelling Onion (Allium cepa) DNA extraction: Perspective of scientific practices through laboratory practiceExtracción de ADN de cebolla (Allium cepa): Perspectiva de las prácticas científicas a través de la práctica de laboratórioExtração de DNA de cebola (Allium cepa): Perspectiva das práticas científicas por meio da prática de laboratórioÁcido desoxirribonucleicoPrática de laboratórioExtração de DNAEnsino médio.Deoxyribonucleic acidLaboratory practiceDNA extractionHigh school.Ácido desoxirribonucleicoPrácticas de laboratorioExtracción de DNABachillerato.DNA represents the structural unit of life, being one of the best-known organic substances. DNA extraction has become a alternative to teaching-learning in Biology classes. In this sense, the objective of this work was to facilitate the learning of high school Biology students, based on an experimental activity involving the extraction of DNA from onions (Allium cepa). The activity was implemented in a practical class laboratory at IFPE-Campus Barreiros in two pedagogical moments. The first moment consisted of a practical class for extracting DNA from A. cepa, using everyday materials. In the second moment, students were invited to evaluate the activity through a questionnaire. From the analysis of the results, it was possible to verify that the proposed activity encouraged motivation, aroused curiosity and facilitated students learning. Furthermore, the evaluation of the activity by the students was considered stimulating by the authors. Finally, in addition to combining theory with practice, this work can be useful as reference material on the DNA molecule.El DNA representa una unidad estructural de la vida, siendo una de las sustancias orgánicas más conhecidas. La extracción de ADN se ha convertido en una alternativa de enseñanza-aprendizaje en las clases de Biología. En este sentido, el objetivo de este trabajo fue facilitar el aprendizaje de estudiantes de Biología, a partir de una actividad experimental que involucra la extracción de ADN de cebolla (Allium cepa). La actividad se implementó en el laboratorio de clases prácticas del IFPE-Campus Barreiros en dos momentos pedagógicos. El primer momento consistió en una clase práctica de extracción de DNA de A. cepa, utilizando materiales cotidianos. En el segundo momento, se invitó a los estudiantes a evaluar la actividad a través de un cuestionario. Del análisis de los resultados se pudo comprobar que la actividad propuesta fomentó la motivación, despertó la curiosidad y facilitó el aprendizaje de los estudiantes. Además, la evaluación de la actividad por los estudiantes fue considerada estimulante por los autores. Finalmente, además de combinar teoría con práctica, este trabajo puede resultar útil como material de referencia sobre la molécula de DNA.O DNA representa a unidade estrutural da vida, sendo uma das substâncias orgânicas mais conhecidas. A extração de DNA tornou-se uma alternativa ao ensino-aprendizagem nas aulas de Biologia. Neste sentido, o objetivo deste trabalho foi facilitar a aprendizagem dos alunos da disciplina de Biologia do Ensino Médio, a partir de uma atividade experimental envolvendo a extração de DNA de cebola (Allium cepa). A atividade foi implementada no laboratório de aulas práticas do IFPE-Campus Barreiros em dois momentos pedagógicos. O primeiro momento, consistiu em uma aula prática para a extração do DNA de A. cepa, usando materiais do cotidiano. No segundo momento, os estudantes foram convidados a avaliar a atividade através de um questionário. A partir da análise dos resultados, foi possível verificar que a atividade proposta incentivou a motivação, despertou à curiosidade e facilitou à aprendizagem dos educandos. Além disso, a avaliação da atividade pelos discentes foi considerada estimuladora pelos autores. Por fim, além de unir a teoria com a prática, este trabalho pode ser útil como um material de consulta sobre a molécula do DNA.Research, Society and Development2023-12-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/4458310.33448/rsd-v12i14.44583Research, Society and Development; Vol. 12 No. 14; e75121444583Research, Society and Development; Vol. 12 Núm. 14; e75121444583Research, Society and Development; v. 12 n. 14; e751214445832525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/44583/35638Copyright (c) 2023 Edmilson Clarindo de Siqueira; Bogdan Doboszewskihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSiqueira, Edmilson Clarindo de Doboszewski, Bogdan 2024-01-01T11:23:38Zoai:ojs.pkp.sfu.ca:article/44583Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-01T11:23:38Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Onion (Allium cepa) DNA extraction: Perspective of scientific practices through laboratory practice
Extracción de ADN de cebolla (Allium cepa): Perspectiva de las prácticas científicas a través de la práctica de laboratório
Extração de DNA de cebola (Allium cepa): Perspectiva das práticas científicas por meio da prática de laboratório
title Onion (Allium cepa) DNA extraction: Perspective of scientific practices through laboratory practice
spellingShingle Onion (Allium cepa) DNA extraction: Perspective of scientific practices through laboratory practice
Siqueira, Edmilson Clarindo de
Ácido desoxirribonucleico
Prática de laboratório
Extração de DNA
Ensino médio.
Deoxyribonucleic acid
Laboratory practice
DNA extraction
High school.
Ácido desoxirribonucleico
Prácticas de laboratorio
Extracción de DNA
Bachillerato.
title_short Onion (Allium cepa) DNA extraction: Perspective of scientific practices through laboratory practice
title_full Onion (Allium cepa) DNA extraction: Perspective of scientific practices through laboratory practice
title_fullStr Onion (Allium cepa) DNA extraction: Perspective of scientific practices through laboratory practice
title_full_unstemmed Onion (Allium cepa) DNA extraction: Perspective of scientific practices through laboratory practice
title_sort Onion (Allium cepa) DNA extraction: Perspective of scientific practices through laboratory practice
author Siqueira, Edmilson Clarindo de
author_facet Siqueira, Edmilson Clarindo de
Doboszewski, Bogdan
author_role author
author2 Doboszewski, Bogdan
author2_role author
dc.contributor.author.fl_str_mv Siqueira, Edmilson Clarindo de
Doboszewski, Bogdan
dc.subject.por.fl_str_mv Ácido desoxirribonucleico
Prática de laboratório
Extração de DNA
Ensino médio.
Deoxyribonucleic acid
Laboratory practice
DNA extraction
High school.
Ácido desoxirribonucleico
Prácticas de laboratorio
Extracción de DNA
Bachillerato.
topic Ácido desoxirribonucleico
Prática de laboratório
Extração de DNA
Ensino médio.
Deoxyribonucleic acid
Laboratory practice
DNA extraction
High school.
Ácido desoxirribonucleico
Prácticas de laboratorio
Extracción de DNA
Bachillerato.
description DNA represents the structural unit of life, being one of the best-known organic substances. DNA extraction has become a alternative to teaching-learning in Biology classes. In this sense, the objective of this work was to facilitate the learning of high school Biology students, based on an experimental activity involving the extraction of DNA from onions (Allium cepa). The activity was implemented in a practical class laboratory at IFPE-Campus Barreiros in two pedagogical moments. The first moment consisted of a practical class for extracting DNA from A. cepa, using everyday materials. In the second moment, students were invited to evaluate the activity through a questionnaire. From the analysis of the results, it was possible to verify that the proposed activity encouraged motivation, aroused curiosity and facilitated students learning. Furthermore, the evaluation of the activity by the students was considered stimulating by the authors. Finally, in addition to combining theory with practice, this work can be useful as reference material on the DNA molecule.
publishDate 2023
dc.date.none.fl_str_mv 2023-12-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/44583
10.33448/rsd-v12i14.44583
url https://rsdjournal.org/index.php/rsd/article/view/44583
identifier_str_mv 10.33448/rsd-v12i14.44583
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/44583/35638
dc.rights.driver.fl_str_mv Copyright (c) 2023 Edmilson Clarindo de Siqueira; Bogdan Doboszewski
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Edmilson Clarindo de Siqueira; Bogdan Doboszewski
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 12 No. 14; e75121444583
Research, Society and Development; Vol. 12 Núm. 14; e75121444583
Research, Society and Development; v. 12 n. 14; e75121444583
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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