In vitro inhibition of Fusarium solani by Trichoderma harzianum and biofertilizer
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/12994 |
Resumo: | Cassava root rot causes significant production losses. Difficulties of management, along with the lack of chemical fungicides officially registered by the Ministry of Agriculture, Livestock and Supply (MAPA), require alternative control methods. This study investigated the in vitro antagonistic activity of Trichoderma harzianum as well as a biological fertilizer MICROGEO® on Fusarium solani. The phytophatogenic strains of F. solani, called F1 and F2 were isolated from rotted cassava tubers and T. harzianum, strain ESALQ 1306, from a biological fungicide. Continuous liquid composting of bovine ruminal content, water and MICROGEO® produced the biological fertilizer. Dual culture method was used at the bioassay with T. harzianum. Sterilized (St) and unsterilized (USt) biological fertilizer were tested in different concentrations (% v/v) diluted in the culture media. Colony diameters were measured daily in order to establish the mycelial growth velocity index, inhibition percentage, aside from the sporulation rate and spore germination percentage. The mycelial growth of F. solani isolates was interrupted after hyphae encounter with T. harzianum, due to the occurrence of mycoparasitism, but without influence on the sporulation rate. Sterilized biological fertilizer induced no biocontrol, whereas the unsterilized product (concentration 2.5%) inhibited approximately 64% and 85% of the mycelial growth of isolates F1 and F2, respectively. Moreover, spore germination declined with increasing concentration. In conclusion, T. harzianum and the unsterilized biofertilizer showed in vitro antagonistic activity on F. solani. |
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In vitro inhibition of Fusarium solani by Trichoderma harzianum and biofertilizerInhibición in vitro de Fusarium solani por Trichocherma harzianum y biofertilizanteInibição in vitro de Fusarium solani por Trichocherma harzianum e biofertilizanteBiocontrolBiofertilizanteMicoparasitismoCrecimiento micelialFusariosis.BiocontroleBiofertilizanteMicoparasitismoCrescimento micelialFusariose.Biological controlBiological fertilizerMycoparasitismMycelium growthFusarium root rot.Cassava root rot causes significant production losses. Difficulties of management, along with the lack of chemical fungicides officially registered by the Ministry of Agriculture, Livestock and Supply (MAPA), require alternative control methods. This study investigated the in vitro antagonistic activity of Trichoderma harzianum as well as a biological fertilizer MICROGEO® on Fusarium solani. The phytophatogenic strains of F. solani, called F1 and F2 were isolated from rotted cassava tubers and T. harzianum, strain ESALQ 1306, from a biological fungicide. Continuous liquid composting of bovine ruminal content, water and MICROGEO® produced the biological fertilizer. Dual culture method was used at the bioassay with T. harzianum. Sterilized (St) and unsterilized (USt) biological fertilizer were tested in different concentrations (% v/v) diluted in the culture media. Colony diameters were measured daily in order to establish the mycelial growth velocity index, inhibition percentage, aside from the sporulation rate and spore germination percentage. The mycelial growth of F. solani isolates was interrupted after hyphae encounter with T. harzianum, due to the occurrence of mycoparasitism, but without influence on the sporulation rate. Sterilized biological fertilizer induced no biocontrol, whereas the unsterilized product (concentration 2.5%) inhibited approximately 64% and 85% of the mycelial growth of isolates F1 and F2, respectively. Moreover, spore germination declined with increasing concentration. In conclusion, T. harzianum and the unsterilized biofertilizer showed in vitro antagonistic activity on F. solani.La pudrición de raíces de yuca provoca pérdidas en su producción. El difícil manejo, la indisponibilidad de fungicidas químicos registrados en el Ministerio de Agricultura, Ganadería y Abastecimiento (MAPA), demandan alternativas de control. El objetivo de este trabajo fuer verificar la actividad antagonista in vitro de Trichoderma harzianum y fertilizante biológico MICROGEO® sobre Fusarium solani. F. solani (F1 y F2) se obtuvieron de raíces de yuca y T. harzianum, cepa ESALQ 1306, de un fungicida biológico. El fertilizante biológico se produjo por compostaje líquido de MICROGEO® con contenido de rumen bovino y agua. Para el bioensayo con T. harzianum se utilizó el método de cultivo pareado. El fertilizante biológico se probó esterilizado (E) y no esterilizado (NE) en diferentes concentraciones diluidas en medio de cultivo. El crecimiento micelial se evaluó midiendo el diámetro de las colonias, obteniendo el índice de crecimiento micelial (IVCM), el porcentaje de inhibición, la tasa de esporulación y el porcentaje de germinación de esporas. El crecimiento micelial de F. solani se interrumpió después del encuentro de sus hifas com T. harzianum por micoparasitismo, pero sin interferencia con la esporulación. El fertilizante biológico esterilizado no promovió el biocontrol, pero su efecto no esterilizado al 2.5% inhibió aproximadamente 64% y 85% del crecimiento de los hongos F1 y F2, respectivamente. Hubo una disminución en la germinación de las esporas com el aumento en la concentración del producto. Se concluye que el hongo T. harzianum y el fertilizante biológico no esterilizado demostraron actividad antagonista in vitro sobre F. solani.A podridão radicular da mandioca ocasiona perdas relevantes em sua produção. O difícil manejo, aliado à indisponibilidade de fungicidas químicos registrados ao Ministério da Agricultura, Pecuária e Abastecimento (MAPA) demandam alternativas de controle. O objetivo deste trabalho foi verificar a atividade antagonista in vitro de Trichoderma harzianum e adubo biológico MICROGEO® sobre Fusarium solani. F. solani (F1 e F2) foram isolados de raízes de mandioca e T. harzianum, linhagem ESALQ 1306, de um fungicida biológico. O adubo biológico foi produzido por compostagem líquida contínua de MICROGEO® com conteúdo ruminal bovino e água. Para o bioensaio com T. harzianum utilizou-se o método de cultura pareada. O adubo biológico foi testado esterilizado (E) e não esterilizado (NE) em diferentes concentrações (% v/v) diluídas nos meios de cultura. Avaliou-se o crescimento micelial pela medição do diâmetro das colônias, obtendo-se o índice de velocidade de crescimento micelial (IVCM), a porcentagem de inibição, a taxa de esporulação e porcentagem de germinação dos esporos. O crescimento micelial de F. solani em cultivo pareado com T. harzianum foi interrompido após o encontro de suas hifas pela ocorrência de micoparasitismo, porém sem interferência na esporulação. O adubo biológico esterilizado não promoveu biocontrole, porém seu efeito não esterilizado à 2,5% inibiu aproximadamente 64% e 85% o crescimento dos fungos F1 e F2, respectivamente. Além disto, observou-se declínio na germinação dos esporos conforme aumento da concentração do produto. Conclui-se que o fungo T. harzianum e o adubo biológico não esterilizado demonstraram atividade antagonista in vitro sobre F. solani.Research, Society and Development2021-03-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1299410.33448/rsd-v10i3.12994Research, Society and Development; Vol. 10 No. 3; e5210312994Research, Society and Development; Vol. 10 Núm. 3; e5210312994Research, Society and Development; v. 10 n. 3; e52103129942525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/12994/11719Copyright (c) 2021 Paula Fernanda de Azevedo; Ana Carolina de Almeida; Rodrigo Domiciano Marques; Christiane Luciana da Costa; Anderson Roberto Benedetti; Luis Eduardo Azevedo Marques Lescano; Mayra Costa da Cruz Gallo de Carvalho; Leopoldo Sussumu Matsumotohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAzevedo, Paula Fernanda deAlmeida, Ana Carolina de Marques, Rodrigo Domiciano Costa, Christiane Luciana da Benedetti, Anderson Roberto Lescano, Luis Eduardo Azevedo Marques Carvalho, Mayra Costa da Cruz Gallo de Matsumoto, Leopoldo Sussumu 2021-03-28T12:03:35Zoai:ojs.pkp.sfu.ca:article/12994Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:34:25.431165Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
In vitro inhibition of Fusarium solani by Trichoderma harzianum and biofertilizer Inhibición in vitro de Fusarium solani por Trichocherma harzianum y biofertilizante Inibição in vitro de Fusarium solani por Trichocherma harzianum e biofertilizante |
title |
In vitro inhibition of Fusarium solani by Trichoderma harzianum and biofertilizer |
spellingShingle |
In vitro inhibition of Fusarium solani by Trichoderma harzianum and biofertilizer Azevedo, Paula Fernanda de Biocontrol Biofertilizante Micoparasitismo Crecimiento micelial Fusariosis. Biocontrole Biofertilizante Micoparasitismo Crescimento micelial Fusariose. Biological control Biological fertilizer Mycoparasitism Mycelium growth Fusarium root rot. |
title_short |
In vitro inhibition of Fusarium solani by Trichoderma harzianum and biofertilizer |
title_full |
In vitro inhibition of Fusarium solani by Trichoderma harzianum and biofertilizer |
title_fullStr |
In vitro inhibition of Fusarium solani by Trichoderma harzianum and biofertilizer |
title_full_unstemmed |
In vitro inhibition of Fusarium solani by Trichoderma harzianum and biofertilizer |
title_sort |
In vitro inhibition of Fusarium solani by Trichoderma harzianum and biofertilizer |
author |
Azevedo, Paula Fernanda de |
author_facet |
Azevedo, Paula Fernanda de Almeida, Ana Carolina de Marques, Rodrigo Domiciano Costa, Christiane Luciana da Benedetti, Anderson Roberto Lescano, Luis Eduardo Azevedo Marques Carvalho, Mayra Costa da Cruz Gallo de Matsumoto, Leopoldo Sussumu |
author_role |
author |
author2 |
Almeida, Ana Carolina de Marques, Rodrigo Domiciano Costa, Christiane Luciana da Benedetti, Anderson Roberto Lescano, Luis Eduardo Azevedo Marques Carvalho, Mayra Costa da Cruz Gallo de Matsumoto, Leopoldo Sussumu |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Azevedo, Paula Fernanda de Almeida, Ana Carolina de Marques, Rodrigo Domiciano Costa, Christiane Luciana da Benedetti, Anderson Roberto Lescano, Luis Eduardo Azevedo Marques Carvalho, Mayra Costa da Cruz Gallo de Matsumoto, Leopoldo Sussumu |
dc.subject.por.fl_str_mv |
Biocontrol Biofertilizante Micoparasitismo Crecimiento micelial Fusariosis. Biocontrole Biofertilizante Micoparasitismo Crescimento micelial Fusariose. Biological control Biological fertilizer Mycoparasitism Mycelium growth Fusarium root rot. |
topic |
Biocontrol Biofertilizante Micoparasitismo Crecimiento micelial Fusariosis. Biocontrole Biofertilizante Micoparasitismo Crescimento micelial Fusariose. Biological control Biological fertilizer Mycoparasitism Mycelium growth Fusarium root rot. |
description |
Cassava root rot causes significant production losses. Difficulties of management, along with the lack of chemical fungicides officially registered by the Ministry of Agriculture, Livestock and Supply (MAPA), require alternative control methods. This study investigated the in vitro antagonistic activity of Trichoderma harzianum as well as a biological fertilizer MICROGEO® on Fusarium solani. The phytophatogenic strains of F. solani, called F1 and F2 were isolated from rotted cassava tubers and T. harzianum, strain ESALQ 1306, from a biological fungicide. Continuous liquid composting of bovine ruminal content, water and MICROGEO® produced the biological fertilizer. Dual culture method was used at the bioassay with T. harzianum. Sterilized (St) and unsterilized (USt) biological fertilizer were tested in different concentrations (% v/v) diluted in the culture media. Colony diameters were measured daily in order to establish the mycelial growth velocity index, inhibition percentage, aside from the sporulation rate and spore germination percentage. The mycelial growth of F. solani isolates was interrupted after hyphae encounter with T. harzianum, due to the occurrence of mycoparasitism, but without influence on the sporulation rate. Sterilized biological fertilizer induced no biocontrol, whereas the unsterilized product (concentration 2.5%) inhibited approximately 64% and 85% of the mycelial growth of isolates F1 and F2, respectively. Moreover, spore germination declined with increasing concentration. In conclusion, T. harzianum and the unsterilized biofertilizer showed in vitro antagonistic activity on F. solani. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-03-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/12994 10.33448/rsd-v10i3.12994 |
url |
https://rsdjournal.org/index.php/rsd/article/view/12994 |
identifier_str_mv |
10.33448/rsd-v10i3.12994 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/12994/11719 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 3; e5210312994 Research, Society and Development; Vol. 10 Núm. 3; e5210312994 Research, Society and Development; v. 10 n. 3; e5210312994 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052832342343680 |