Aspiration cytology of popliteal lymph nodes as a diagnostic method of canine visceral leishmaniasis (CVL) in areas of socioeconomic vulnerability
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
DOI: | 10.33448/rsd-v10i5.14457 |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/14457 |
Resumo: | Leishmaniasis is an endemic disease in Goiás and has high lethality owing to its high zoonotic potential. In dogs, the disease manifests based on the parasitic load, with clinical presentation as the starting for diagnosis. Diagnostic methods are used to confirm the disease, with emphasis on lymph node aspiration cytology, which is a high-standard technique owing to its low cost, ease of performance, and high specificity. The aim of this study was to demonstrate aspiration lymph node cytology as a diagnostic method for canine visceral leishmaniasis (CVL) in areas containing low-income populations with poor access to molecular tests. Three dogs with suspected CVL were studied at Veterinary Hospital of the Federal University of Jataí, in which popliteal lymph node cytology was performed, owing to easy access, and screening and confirmatory examinations were undertaken by the Central Public Health Laboratory. The results showed three positive animals in the cytology and only one positive in the other tests; therefore, there was the possibility of the occurrence of false negatives in serological tests given the time required for seroconversion. Thus, cytology is an important diagnostic technique in areas where molecular tests are not available. |
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Research, Society and Development |
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Aspiration cytology of popliteal lymph nodes as a diagnostic method of canine visceral leishmaniasis (CVL) in areas of socioeconomic vulnerabilityCitología por aspiración de ganglios linfáticos poplíteos como método diagnóstico de leishmaniasis visceral canina (LVC) en áreas de vulnerabilidad socioeconómicaCitologia aspirativa de linfonodos poplíteos como método diagnóstico de leishmaniose visceral canina (LVC) em áreas de vulnerabilidade socioeconômicaAmastigotasLeishmania sppMacrófagosOnicogrifose. AmastigotesLeishmania sppMacrófagosOnicogrifosis. AmastigotesLeishmania sppMacrophagesOnychogryphosis. Leishmaniasis is an endemic disease in Goiás and has high lethality owing to its high zoonotic potential. In dogs, the disease manifests based on the parasitic load, with clinical presentation as the starting for diagnosis. Diagnostic methods are used to confirm the disease, with emphasis on lymph node aspiration cytology, which is a high-standard technique owing to its low cost, ease of performance, and high specificity. The aim of this study was to demonstrate aspiration lymph node cytology as a diagnostic method for canine visceral leishmaniasis (CVL) in areas containing low-income populations with poor access to molecular tests. Three dogs with suspected CVL were studied at Veterinary Hospital of the Federal University of Jataí, in which popliteal lymph node cytology was performed, owing to easy access, and screening and confirmatory examinations were undertaken by the Central Public Health Laboratory. The results showed three positive animals in the cytology and only one positive in the other tests; therefore, there was the possibility of the occurrence of false negatives in serological tests given the time required for seroconversion. Thus, cytology is an important diagnostic technique in areas where molecular tests are not available.La leishmaniasis es una enfermedad endémica en Goiás y tiene una alta letalidad debido a su alto potencial zoonótico. En los perros, la enfermedad se manifiesta en función de la carga parasitaria, con la presentación clínica como principio de diagnóstico. Se utilizan métodos de diagnóstico para confirmar la enfermedad, con énfasis en la citología por aspiración de ganglios linfáticos, que es una técnica de alto estándar debido a su bajo costo, facilidad de realización y alta especificidad. El objetivo de este estudio fue demostrar la citología por aspiración de ganglios linfáticos como método de diagnóstico para la leishmaniasis visceral canina (CVL) en áreas que contienen poblaciones de bajos ingresos con poco acceso a pruebas moleculares. Se estudiaron tres perros con sospecha de LVC en el Hospital Veterinario de la Universidad Federal de Jataí, en el que se realizó citología por aspiración de ganglio linfático poplíteo, por su fácil acceso, y se realizaron pruebas de cribado y confirmación por parte del Laboratorio Central de Salud Pública. Los resultados arrojaron tres animales positivos en la citología y solo uno positivo en las demás pruebas, lo que demuestra la posibilidad de la ocurrencia de falsos negativos en las pruebas serológicas, dado el tiempo necesario para la seroconversión. Por lo tanto, la citología es una técnica de diagnóstico importante en áreas donde las pruebas moleculares no están disponibles.A leishmaniose é uma doença endêmica em Goiás e apresenta alta letalidade devido ao seu alto potencial zoonótico. Em cães, a doença se manifesta baseada na carga parasitária, com apresentação clínica como princípio para o diagnóstico. Métodos de diagnóstico são utilizados para confirmar a doença, com ênfase na citologia de aspiração de linfonodos, que é uma técnica de alto padrão devido ao seu baixo custo, facilidade de desempenho e alta especificidade. O objetivo deste estudo foi demonstrar a citologia de aspiração de linfonodos como um método diagnóstico para leishmaniose visceral canina (LVC) em áreas contendo populações de baixa renda com pouco acesso a testes moleculares. Três cães com suspeita de LVC foram estudados no Hospital Veterinário da Universidade Federal de Jataí, no qual foi realizada citologia de aspiração de linfonodo poplíteo, devido ao fácil acesso, e exames de triagem e confirmação foram realizados pelo Laboratório Central de Saúde Pública. Os resultados mostraram três animais positivos na citologia e apenas um positivo nos outros testes, demonstrando a possibilidade de ocorrência de falsos negativos em testes sorológicos, dado o tempo necessário para soroconversão. Assim, a citologia é uma importante técnica de diagnóstico em áreas onde os testes moleculares não estão disponíveis. Research, Society and Development2021-04-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1445710.33448/rsd-v10i5.14457Research, Society and Development; Vol. 10 No. 5; e2310514457Research, Society and Development; Vol. 10 Núm. 5; e2310514457Research, Society and Development; v. 10 n. 5; e23105144572525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/14457/13090Copyright (c) 2021 Antônio Carlos Severino Neto; Gabriel Lopes Germano; Thâmara Rossi Martins da Silva; Luiz Antônio de Jesus Sousa; Priscila Gomes de Oliveira; Reiner Silveira de Moraes; Dirceu Guilherme de Sousa Ramoshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSeverino Neto, Antônio CarlosGermano, Gabriel LopesSilva, Thâmara Rossi Martins daSousa, Luiz Antônio de JesusOliveira, Priscila Gomes deMoraes, Reiner Silveira deRamos, Dirceu Guilherme de Sousa2021-05-17T18:20:49Zoai:ojs.pkp.sfu.ca:article/14457Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:35:34.947567Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Aspiration cytology of popliteal lymph nodes as a diagnostic method of canine visceral leishmaniasis (CVL) in areas of socioeconomic vulnerability Citología por aspiración de ganglios linfáticos poplíteos como método diagnóstico de leishmaniasis visceral canina (LVC) en áreas de vulnerabilidad socioeconómica Citologia aspirativa de linfonodos poplíteos como método diagnóstico de leishmaniose visceral canina (LVC) em áreas de vulnerabilidade socioeconômica |
title |
Aspiration cytology of popliteal lymph nodes as a diagnostic method of canine visceral leishmaniasis (CVL) in areas of socioeconomic vulnerability |
spellingShingle |
Aspiration cytology of popliteal lymph nodes as a diagnostic method of canine visceral leishmaniasis (CVL) in areas of socioeconomic vulnerability Aspiration cytology of popliteal lymph nodes as a diagnostic method of canine visceral leishmaniasis (CVL) in areas of socioeconomic vulnerability Severino Neto, Antônio Carlos Amastigotas Leishmania spp Macrófagos Onicogrifose. Amastigotes Leishmania spp Macrófagos Onicogrifosis. Amastigotes Leishmania spp Macrophages Onychogryphosis. Severino Neto, Antônio Carlos Amastigotas Leishmania spp Macrófagos Onicogrifose. Amastigotes Leishmania spp Macrófagos Onicogrifosis. Amastigotes Leishmania spp Macrophages Onychogryphosis. |
title_short |
Aspiration cytology of popliteal lymph nodes as a diagnostic method of canine visceral leishmaniasis (CVL) in areas of socioeconomic vulnerability |
title_full |
Aspiration cytology of popliteal lymph nodes as a diagnostic method of canine visceral leishmaniasis (CVL) in areas of socioeconomic vulnerability |
title_fullStr |
Aspiration cytology of popliteal lymph nodes as a diagnostic method of canine visceral leishmaniasis (CVL) in areas of socioeconomic vulnerability Aspiration cytology of popliteal lymph nodes as a diagnostic method of canine visceral leishmaniasis (CVL) in areas of socioeconomic vulnerability |
title_full_unstemmed |
Aspiration cytology of popliteal lymph nodes as a diagnostic method of canine visceral leishmaniasis (CVL) in areas of socioeconomic vulnerability Aspiration cytology of popliteal lymph nodes as a diagnostic method of canine visceral leishmaniasis (CVL) in areas of socioeconomic vulnerability |
title_sort |
Aspiration cytology of popliteal lymph nodes as a diagnostic method of canine visceral leishmaniasis (CVL) in areas of socioeconomic vulnerability |
author |
Severino Neto, Antônio Carlos |
author_facet |
Severino Neto, Antônio Carlos Severino Neto, Antônio Carlos Germano, Gabriel Lopes Silva, Thâmara Rossi Martins da Sousa, Luiz Antônio de Jesus Oliveira, Priscila Gomes de Moraes, Reiner Silveira de Ramos, Dirceu Guilherme de Sousa Germano, Gabriel Lopes Silva, Thâmara Rossi Martins da Sousa, Luiz Antônio de Jesus Oliveira, Priscila Gomes de Moraes, Reiner Silveira de Ramos, Dirceu Guilherme de Sousa |
author_role |
author |
author2 |
Germano, Gabriel Lopes Silva, Thâmara Rossi Martins da Sousa, Luiz Antônio de Jesus Oliveira, Priscila Gomes de Moraes, Reiner Silveira de Ramos, Dirceu Guilherme de Sousa |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Severino Neto, Antônio Carlos Germano, Gabriel Lopes Silva, Thâmara Rossi Martins da Sousa, Luiz Antônio de Jesus Oliveira, Priscila Gomes de Moraes, Reiner Silveira de Ramos, Dirceu Guilherme de Sousa |
dc.subject.por.fl_str_mv |
Amastigotas Leishmania spp Macrófagos Onicogrifose. Amastigotes Leishmania spp Macrófagos Onicogrifosis. Amastigotes Leishmania spp Macrophages Onychogryphosis. |
topic |
Amastigotas Leishmania spp Macrófagos Onicogrifose. Amastigotes Leishmania spp Macrófagos Onicogrifosis. Amastigotes Leishmania spp Macrophages Onychogryphosis. |
description |
Leishmaniasis is an endemic disease in Goiás and has high lethality owing to its high zoonotic potential. In dogs, the disease manifests based on the parasitic load, with clinical presentation as the starting for diagnosis. Diagnostic methods are used to confirm the disease, with emphasis on lymph node aspiration cytology, which is a high-standard technique owing to its low cost, ease of performance, and high specificity. The aim of this study was to demonstrate aspiration lymph node cytology as a diagnostic method for canine visceral leishmaniasis (CVL) in areas containing low-income populations with poor access to molecular tests. Three dogs with suspected CVL were studied at Veterinary Hospital of the Federal University of Jataí, in which popliteal lymph node cytology was performed, owing to easy access, and screening and confirmatory examinations were undertaken by the Central Public Health Laboratory. The results showed three positive animals in the cytology and only one positive in the other tests; therefore, there was the possibility of the occurrence of false negatives in serological tests given the time required for seroconversion. Thus, cytology is an important diagnostic technique in areas where molecular tests are not available. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-04-26 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/14457 10.33448/rsd-v10i5.14457 |
url |
https://rsdjournal.org/index.php/rsd/article/view/14457 |
identifier_str_mv |
10.33448/rsd-v10i5.14457 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/14457/13090 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 5; e2310514457 Research, Society and Development; Vol. 10 Núm. 5; e2310514457 Research, Society and Development; v. 10 n. 5; e2310514457 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1822178499051388928 |
dc.identifier.doi.none.fl_str_mv |
10.33448/rsd-v10i5.14457 |