Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/14790 |
Resumo: | The bone repair process, among other physiological mechanisms, occurs in a harmonious manner throughout the body. However, the presence of some deleterious factors such as surgery, trauma or pathology, can interfere with the physiology of bone remodeling. It´s Known that the synergistic antimicrobial action between drugs and biomaterials can help and favor osteogenesis after grafting surgery. Therefore, this study aims to develop chitosan / hydroxyapatite scaffolds with amoxicillin for bone repair in the oral cavity. For this, raw materials were selected and characterized and scaffolds were made by the processes of solubilization, dispersion and lyophilization. The characterizations were made by optical microscopy, scanning electron microscopy, eenergy dispersive spectrometer, swelling potential, degradation analysis, apparent porosity test and in vitro cell cytotoxicity. The scaffolds produced in this research showed to have not only adequate physical characteristics but also chemical and biological ones. It was as well as detected a reproducible methodology that resulted in a biomaterial with morphology that provided a high degree of swelling, porosity, satisfactory degradation and the presence of amoxicillin which was confirmed by the eenergy dispersive spectrometer. Nevertheless, the scaffolds with 30% hydroxyapatite showed the best results for in vitro cell viability. So, it is possible to conclude that the scaffolds produced show successful characteristics for bone repair in the oral cavity. |
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Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair Andamios de quitosano e hidroxiapatita con amoxicilina para la reparación óseaScaffolds de quitosana e hidroxiapatita com amoxicilina para reparação ósseaQuitosanaHidroxiapatitaScaffoldAmoxicilinaSistemas de liberação de medicamentos.ChitosanHydroxyapatiteScaffoldsAmoxicillinDrug delivery systems.QuitosanoHidroxiapatitaAndamioAmoxicilinaSistemas de liberación de medicamento.The bone repair process, among other physiological mechanisms, occurs in a harmonious manner throughout the body. However, the presence of some deleterious factors such as surgery, trauma or pathology, can interfere with the physiology of bone remodeling. It´s Known that the synergistic antimicrobial action between drugs and biomaterials can help and favor osteogenesis after grafting surgery. Therefore, this study aims to develop chitosan / hydroxyapatite scaffolds with amoxicillin for bone repair in the oral cavity. For this, raw materials were selected and characterized and scaffolds were made by the processes of solubilization, dispersion and lyophilization. The characterizations were made by optical microscopy, scanning electron microscopy, eenergy dispersive spectrometer, swelling potential, degradation analysis, apparent porosity test and in vitro cell cytotoxicity. The scaffolds produced in this research showed to have not only adequate physical characteristics but also chemical and biological ones. It was as well as detected a reproducible methodology that resulted in a biomaterial with morphology that provided a high degree of swelling, porosity, satisfactory degradation and the presence of amoxicillin which was confirmed by the eenergy dispersive spectrometer. Nevertheless, the scaffolds with 30% hydroxyapatite showed the best results for in vitro cell viability. So, it is possible to conclude that the scaffolds produced show successful characteristics for bone repair in the oral cavity.El proceso de reparación ósea, entre otros mecanismos fisiológicos, se produce de forma armoniosa en todo el organismo. Sin embargo, la presencia de algunos factores deletéreos, como cirugía, traumatismo o patología, puede interferir con la fisiología de la remodelación ósea. Se sabe que la acción antimicrobiana sinérgica entre fármacos y biomateriales puede ayudar y favorecer la osteogénesis tras la cirugía de injerto. Por lo tanto, este estudio tiene como objetivo desarrollar andamios de quitosano / hidroxiapatita con amoxicilina para la reparación ósea en la cavidad oral. Para ello, se seleccionaron y caracterizaron las materias primas y se confeccionaron los andamios mediante los procesos de solubilización, dispersión y liofilización. Las caracterizaciones se realizaron mediante microscopía óptica, microscopía electrónica de barrido, espectrómetro de dispersión de energía, potencial de hinchamiento, análisis de biodegradación, prueba de porosidad aparente y citotoxicidad celular in vitro. Los andamios producidos en esta investigación mostraron no solo características físicas adecuadas, sino también químicas y biológicas. También se detectó una metodología reproducible, lo que resultó en un biomaterial con morfología que aportó un alto grado de hinchamiento, porosidad, degradación satisfactoria y la presencia de amoxicilina lo cual fue confirmado por el espectrómetro de dispersión de energía. Entre las muestras, los andamios con 30% de hidroxiapatita mostraron los mejores resultados para la viabilidad celular in vitro. Así, es posible concluir que los andamios producidos tienen características exitosas para la reparación ósea en la cavidad bucal.O processo reparatório ósseo, entre outros mecanismos fisiológicos, ocorre de forma harmoniosa em todo o corpo. No entanto, a presença de alguns fatores deletérios, como cirurgia, trauma ou patologia, pode interferir na fisiologia da remodelação óssea. Sabe-se que a ação antimicrobiana sinérgica entre fármacos e biomateriais pode auxiliar e favorecer a osteogênese após cirurgia de enxerto. Assim, este estudo tem como objetivo desenvolver scaffolds de quitosana / hidroxiapatita com amoxicilina para reparo ósseo na cavidade oral. Para isso, as matérias-primas foram selecionadas e caracterizadas e os scaffolds foram confeccionados pelos processos de solubilização, dispersão e liofilização. As caracterizações foram feitas por microscopia ótica, microscopia eletrônica de varredura, espectrômetro dispersivo de energia, potencial de entumecimento, análise de biodegradação, teste de porosidade aparente e citotoxicidade celular in vitro. Os scaffolds produzidos nesta pesquisa mostraram características não apenas físicas adequadas, mas também químicas e biológicas. Foi também detectada uma metodologia reprodutível que resultou em um biomaterial com morfologia que proporcionou um alto grau de intumescimento, porosidade, degradação satisfatória e a presença de amoxicilina que foi confirmada pelo espectrômetro dispersivo de energia. Dentre as amostras, os scaffolds com 30% de hidroxiapatita apresentaram os melhores resultados para viabilidade celular in vitro. Assim, é possível concluir que os scaffolds produzidos apresentam características de sucesso para reparação ósseo na cavidade oral.Research, Society and Development2021-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1479010.33448/rsd-v10i5.14790Research, Society and Development; Vol. 10 No. 5; e13410514790Research, Society and Development; Vol. 10 Núm. 5; e13410514790Research, Society and Development; v. 10 n. 5; e134105147902525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/14790/13248Copyright (c) 2021 Rosemary Cunha de Oliveira Ponciano; Ana Cristina Figueiredo de Melo Costa; Rossembérg Cardoso Barbosa ; Marcus Vinícius Lia Fook ; João José Poncianohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessPonciano, Rosemary Cunha de Oliveira Costa, Ana Cristina Figueiredo de Melo Barbosa , Rossembérg Cardoso Fook , Marcus Vinícius LiaPonciano, João José2021-05-17T18:20:49Zoai:ojs.pkp.sfu.ca:article/14790Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:35:47.884241Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair Andamios de quitosano e hidroxiapatita con amoxicilina para la reparación ósea Scaffolds de quitosana e hidroxiapatita com amoxicilina para reparação óssea |
title |
Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair |
spellingShingle |
Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair Ponciano, Rosemary Cunha de Oliveira Quitosana Hidroxiapatita Scaffold Amoxicilina Sistemas de liberação de medicamentos. Chitosan Hydroxyapatite Scaffolds Amoxicillin Drug delivery systems. Quitosano Hidroxiapatita Andamio Amoxicilina Sistemas de liberación de medicamento. |
title_short |
Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair |
title_full |
Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair |
title_fullStr |
Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair |
title_full_unstemmed |
Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair |
title_sort |
Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair |
author |
Ponciano, Rosemary Cunha de Oliveira |
author_facet |
Ponciano, Rosemary Cunha de Oliveira Costa, Ana Cristina Figueiredo de Melo Barbosa , Rossembérg Cardoso Fook , Marcus Vinícius Lia Ponciano, João José |
author_role |
author |
author2 |
Costa, Ana Cristina Figueiredo de Melo Barbosa , Rossembérg Cardoso Fook , Marcus Vinícius Lia Ponciano, João José |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Ponciano, Rosemary Cunha de Oliveira Costa, Ana Cristina Figueiredo de Melo Barbosa , Rossembérg Cardoso Fook , Marcus Vinícius Lia Ponciano, João José |
dc.subject.por.fl_str_mv |
Quitosana Hidroxiapatita Scaffold Amoxicilina Sistemas de liberação de medicamentos. Chitosan Hydroxyapatite Scaffolds Amoxicillin Drug delivery systems. Quitosano Hidroxiapatita Andamio Amoxicilina Sistemas de liberación de medicamento. |
topic |
Quitosana Hidroxiapatita Scaffold Amoxicilina Sistemas de liberação de medicamentos. Chitosan Hydroxyapatite Scaffolds Amoxicillin Drug delivery systems. Quitosano Hidroxiapatita Andamio Amoxicilina Sistemas de liberación de medicamento. |
description |
The bone repair process, among other physiological mechanisms, occurs in a harmonious manner throughout the body. However, the presence of some deleterious factors such as surgery, trauma or pathology, can interfere with the physiology of bone remodeling. It´s Known that the synergistic antimicrobial action between drugs and biomaterials can help and favor osteogenesis after grafting surgery. Therefore, this study aims to develop chitosan / hydroxyapatite scaffolds with amoxicillin for bone repair in the oral cavity. For this, raw materials were selected and characterized and scaffolds were made by the processes of solubilization, dispersion and lyophilization. The characterizations were made by optical microscopy, scanning electron microscopy, eenergy dispersive spectrometer, swelling potential, degradation analysis, apparent porosity test and in vitro cell cytotoxicity. The scaffolds produced in this research showed to have not only adequate physical characteristics but also chemical and biological ones. It was as well as detected a reproducible methodology that resulted in a biomaterial with morphology that provided a high degree of swelling, porosity, satisfactory degradation and the presence of amoxicillin which was confirmed by the eenergy dispersive spectrometer. Nevertheless, the scaffolds with 30% hydroxyapatite showed the best results for in vitro cell viability. So, it is possible to conclude that the scaffolds produced show successful characteristics for bone repair in the oral cavity. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-05-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/14790 10.33448/rsd-v10i5.14790 |
url |
https://rsdjournal.org/index.php/rsd/article/view/14790 |
identifier_str_mv |
10.33448/rsd-v10i5.14790 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/14790/13248 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 5; e13410514790 Research, Society and Development; Vol. 10 Núm. 5; e13410514790 Research, Society and Development; v. 10 n. 5; e13410514790 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052676035313664 |