Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair

Detalhes bibliográficos
Autor(a) principal: Ponciano, Rosemary Cunha de Oliveira
Data de Publicação: 2021
Outros Autores: Costa, Ana Cristina Figueiredo de Melo, Barbosa , Rossembérg Cardoso, Fook , Marcus Vinícius Lia, Ponciano, João José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/14790
Resumo: The bone repair process, among other physiological mechanisms, occurs in a harmonious manner throughout the body. However, the presence of some deleterious factors such as surgery, trauma or pathology, can interfere with the physiology of bone remodeling. It´s Known that the synergistic antimicrobial action between drugs and biomaterials can help and favor osteogenesis after grafting surgery. Therefore, this study aims to develop chitosan / hydroxyapatite scaffolds with amoxicillin for bone repair in the oral cavity. For this, raw materials were selected and characterized and scaffolds were made by the processes of solubilization, dispersion and lyophilization. The characterizations were made by optical microscopy, scanning electron microscopy, eenergy dispersive spectrometer, swelling potential, degradation analysis, apparent porosity test and in vitro cell cytotoxicity. The scaffolds produced in this research showed to have not only adequate physical characteristics but also chemical and biological ones. It was as well as detected a reproducible methodology that resulted in a biomaterial with morphology that provided a high degree of swelling, porosity, satisfactory degradation and the presence of amoxicillin which was confirmed by the eenergy dispersive spectrometer. Nevertheless, the scaffolds with 30% hydroxyapatite showed the best results for in vitro cell viability. So, it is possible to conclude that the scaffolds produced show successful characteristics for bone repair in the oral cavity.
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spelling Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair Andamios de quitosano e hidroxiapatita con amoxicilina para la reparación óseaScaffolds de quitosana e hidroxiapatita com amoxicilina para reparação ósseaQuitosanaHidroxiapatitaScaffoldAmoxicilinaSistemas de liberação de medicamentos.ChitosanHydroxyapatiteScaffoldsAmoxicillinDrug delivery systems.QuitosanoHidroxiapatitaAndamioAmoxicilinaSistemas de liberación de medicamento.The bone repair process, among other physiological mechanisms, occurs in a harmonious manner throughout the body. However, the presence of some deleterious factors such as surgery, trauma or pathology, can interfere with the physiology of bone remodeling. It´s Known that the synergistic antimicrobial action between drugs and biomaterials can help and favor osteogenesis after grafting surgery. Therefore, this study aims to develop chitosan / hydroxyapatite scaffolds with amoxicillin for bone repair in the oral cavity. For this, raw materials were selected and characterized and scaffolds were made by the processes of solubilization, dispersion and lyophilization. The characterizations were made by optical microscopy, scanning electron microscopy, eenergy dispersive spectrometer, swelling potential, degradation analysis, apparent porosity test and in vitro cell cytotoxicity. The scaffolds produced in this research showed to have not only adequate physical characteristics but also chemical and biological ones. It was as well as detected a reproducible methodology that resulted in a biomaterial with morphology that provided a high degree of swelling, porosity, satisfactory degradation and the presence of amoxicillin which was confirmed by the eenergy dispersive spectrometer. Nevertheless, the scaffolds with 30% hydroxyapatite showed the best results for in vitro cell viability. So, it is possible to conclude that the scaffolds produced show successful characteristics for bone repair in the oral cavity.El proceso de reparación ósea, entre otros mecanismos fisiológicos, se produce de forma armoniosa en todo el organismo. Sin embargo, la presencia de algunos factores deletéreos, como cirugía, traumatismo o patología, puede interferir con la fisiología de la remodelación ósea. Se sabe que la acción antimicrobiana sinérgica entre fármacos y biomateriales puede ayudar y favorecer la osteogénesis tras la cirugía de injerto. Por lo tanto, este estudio tiene como objetivo desarrollar andamios de quitosano / hidroxiapatita con amoxicilina para la reparación ósea en la cavidad oral. Para ello, se seleccionaron y caracterizaron las materias primas y se confeccionaron los andamios mediante los procesos de solubilización, dispersión y liofilización. Las caracterizaciones se realizaron mediante microscopía óptica, microscopía electrónica de barrido, espectrómetro de dispersión de energía, potencial de hinchamiento, análisis de biodegradación, prueba de porosidad aparente y citotoxicidad celular in vitro. Los andamios producidos en esta investigación mostraron no solo características físicas adecuadas, sino también químicas y biológicas. También se detectó una metodología reproducible, lo que resultó en un biomaterial con morfología que aportó un alto grado de hinchamiento, porosidad, degradación satisfactoria y la presencia de amoxicilina lo cual fue confirmado por el espectrómetro de dispersión de energía. Entre las muestras, los andamios con 30% de hidroxiapatita mostraron los mejores resultados para la viabilidad celular in vitro. Así, es posible concluir que los andamios producidos tienen características exitosas para la reparación ósea en la cavidad bucal.O processo reparatório ósseo, entre outros mecanismos fisiológicos, ocorre de forma harmoniosa em todo o corpo. No entanto, a presença de alguns fatores deletérios, como cirurgia, trauma ou patologia, pode interferir na fisiologia da remodelação óssea. Sabe-se que a ação antimicrobiana sinérgica entre fármacos e biomateriais pode auxiliar e favorecer a osteogênese após cirurgia de enxerto. Assim, este estudo tem como objetivo desenvolver scaffolds de quitosana / hidroxiapatita com amoxicilina para reparo ósseo na cavidade oral. Para isso, as matérias-primas foram selecionadas e caracterizadas e os scaffolds foram confeccionados pelos processos de solubilização, dispersão e liofilização. As caracterizações foram feitas por microscopia ótica, microscopia eletrônica de varredura, espectrômetro dispersivo de energia, potencial de entumecimento, análise de biodegradação, teste de porosidade aparente e citotoxicidade celular in vitro. Os scaffolds produzidos nesta pesquisa mostraram características não apenas físicas adequadas, mas também químicas e biológicas. Foi também detectada uma metodologia reprodutível que resultou em um biomaterial com morfologia que proporcionou um alto grau de intumescimento, porosidade, degradação satisfatória e a presença de amoxicilina que foi confirmada pelo espectrômetro dispersivo de energia. Dentre as amostras, os scaffolds com 30% de hidroxiapatita apresentaram os melhores resultados para viabilidade celular in vitro. Assim, é possível concluir que os scaffolds produzidos apresentam características de sucesso para reparação ósseo na cavidade oral.Research, Society and Development2021-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1479010.33448/rsd-v10i5.14790Research, Society and Development; Vol. 10 No. 5; e13410514790Research, Society and Development; Vol. 10 Núm. 5; e13410514790Research, Society and Development; v. 10 n. 5; e134105147902525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/14790/13248Copyright (c) 2021 Rosemary Cunha de Oliveira Ponciano; Ana Cristina Figueiredo de Melo Costa; Rossembérg Cardoso Barbosa ; Marcus Vinícius Lia Fook ; João José Poncianohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessPonciano, Rosemary Cunha de Oliveira Costa, Ana Cristina Figueiredo de Melo Barbosa , Rossembérg Cardoso Fook , Marcus Vinícius LiaPonciano, João José2021-05-17T18:20:49Zoai:ojs.pkp.sfu.ca:article/14790Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:35:47.884241Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair
Andamios de quitosano e hidroxiapatita con amoxicilina para la reparación ósea
Scaffolds de quitosana e hidroxiapatita com amoxicilina para reparação óssea
title Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair
spellingShingle Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair
Ponciano, Rosemary Cunha de Oliveira
Quitosana
Hidroxiapatita
Scaffold
Amoxicilina
Sistemas de liberação de medicamentos.
Chitosan
Hydroxyapatite
Scaffolds
Amoxicillin
Drug delivery systems.
Quitosano
Hidroxiapatita
Andamio
Amoxicilina
Sistemas de liberación de medicamento.
title_short Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair
title_full Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair
title_fullStr Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair
title_full_unstemmed Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair
title_sort Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair
author Ponciano, Rosemary Cunha de Oliveira
author_facet Ponciano, Rosemary Cunha de Oliveira
Costa, Ana Cristina Figueiredo de Melo
Barbosa , Rossembérg Cardoso
Fook , Marcus Vinícius Lia
Ponciano, João José
author_role author
author2 Costa, Ana Cristina Figueiredo de Melo
Barbosa , Rossembérg Cardoso
Fook , Marcus Vinícius Lia
Ponciano, João José
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ponciano, Rosemary Cunha de Oliveira
Costa, Ana Cristina Figueiredo de Melo
Barbosa , Rossembérg Cardoso
Fook , Marcus Vinícius Lia
Ponciano, João José
dc.subject.por.fl_str_mv Quitosana
Hidroxiapatita
Scaffold
Amoxicilina
Sistemas de liberação de medicamentos.
Chitosan
Hydroxyapatite
Scaffolds
Amoxicillin
Drug delivery systems.
Quitosano
Hidroxiapatita
Andamio
Amoxicilina
Sistemas de liberación de medicamento.
topic Quitosana
Hidroxiapatita
Scaffold
Amoxicilina
Sistemas de liberação de medicamentos.
Chitosan
Hydroxyapatite
Scaffolds
Amoxicillin
Drug delivery systems.
Quitosano
Hidroxiapatita
Andamio
Amoxicilina
Sistemas de liberación de medicamento.
description The bone repair process, among other physiological mechanisms, occurs in a harmonious manner throughout the body. However, the presence of some deleterious factors such as surgery, trauma or pathology, can interfere with the physiology of bone remodeling. It´s Known that the synergistic antimicrobial action between drugs and biomaterials can help and favor osteogenesis after grafting surgery. Therefore, this study aims to develop chitosan / hydroxyapatite scaffolds with amoxicillin for bone repair in the oral cavity. For this, raw materials were selected and characterized and scaffolds were made by the processes of solubilization, dispersion and lyophilization. The characterizations were made by optical microscopy, scanning electron microscopy, eenergy dispersive spectrometer, swelling potential, degradation analysis, apparent porosity test and in vitro cell cytotoxicity. The scaffolds produced in this research showed to have not only adequate physical characteristics but also chemical and biological ones. It was as well as detected a reproducible methodology that resulted in a biomaterial with morphology that provided a high degree of swelling, porosity, satisfactory degradation and the presence of amoxicillin which was confirmed by the eenergy dispersive spectrometer. Nevertheless, the scaffolds with 30% hydroxyapatite showed the best results for in vitro cell viability. So, it is possible to conclude that the scaffolds produced show successful characteristics for bone repair in the oral cavity.
publishDate 2021
dc.date.none.fl_str_mv 2021-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/14790
10.33448/rsd-v10i5.14790
url https://rsdjournal.org/index.php/rsd/article/view/14790
identifier_str_mv 10.33448/rsd-v10i5.14790
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/14790/13248
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 5; e13410514790
Research, Society and Development; Vol. 10 Núm. 5; e13410514790
Research, Society and Development; v. 10 n. 5; e13410514790
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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