Immunohistochemical expression of macrophages in chronic periapical lesions

Detalhes bibliográficos
Autor(a) principal: Farias, Zilda Betânia Barbosa Medeiros de
Data de Publicação: 2021
Outros Autores: Cavalcante, Jade Souza, Lima, Anne Caroline de, Andrade, Emanuel Savio de Souza, Silveira, Márcia Maria Fonseca da, Sobral, Ana Paula Veras
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/16622
Resumo: Objective: To analyze the expression of macrophages in periapical granulomas (PGs) and radicular cysts (RCs). Methodology: We selected 264 cases of chronic periapical lesions stored at the Laboratory of Pathology, Dental School of Pernambuco (FOP)/UPE, including 89 PGs and 175 RCs. Seventy-nine cases, 23 PGs and 56 RCs, were submitted to immunohistochemical analysis by the streptavidin-biotin method using anti-CD68 antibody. The chi-square and Fisher’s exact tests were used to determine the existence of associations with categorical and clinical variables, adopting a 95% confidence level (p≤0.05). Results: We observed immunoexpression of CD68 in 83% of cases. The number of CD68+ cells (score 3 or 4) was higher in PGs, with predominantly intense staining. Significant differences in the number of CD68+ cells and staining intensity were observed between the chronic periapical lesions analyzed. Conclusion: The presence of CD68+ cells in periapical tissues may indicate the development, maintenance and/or severity of the inflammation-mediated immune response, which is more intense in PG.
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spelling Immunohistochemical expression of macrophages in chronic periapical lesionsExpresión inmunohistoquímica de macrófagos en lesiones periapicales crónicasExpressão imunohistoquímica de macrófagos em lesões periapicais crônicasGranuloma periapicalCisto radicularImuno-histoquímicaMacrófagos CD68.Granuloma periapicalQuiste radicularInmunohistoquímicaMacrófagos CD68.Periapical granulomaRadicular cystImmunohistochemistryCD68 macrophage.Objective: To analyze the expression of macrophages in periapical granulomas (PGs) and radicular cysts (RCs). Methodology: We selected 264 cases of chronic periapical lesions stored at the Laboratory of Pathology, Dental School of Pernambuco (FOP)/UPE, including 89 PGs and 175 RCs. Seventy-nine cases, 23 PGs and 56 RCs, were submitted to immunohistochemical analysis by the streptavidin-biotin method using anti-CD68 antibody. The chi-square and Fisher’s exact tests were used to determine the existence of associations with categorical and clinical variables, adopting a 95% confidence level (p≤0.05). Results: We observed immunoexpression of CD68 in 83% of cases. The number of CD68+ cells (score 3 or 4) was higher in PGs, with predominantly intense staining. Significant differences in the number of CD68+ cells and staining intensity were observed between the chronic periapical lesions analyzed. Conclusion: The presence of CD68+ cells in periapical tissues may indicate the development, maintenance and/or severity of the inflammation-mediated immune response, which is more intense in PG.Objetivo: Analizar la expresión de macrófagos en granulomas periapicales y quistes radiculares. Metodología: Fueron seleccionados 264 casos de lesiones periapicales crónicas de los cuales 89 eran granulomas periapicales (GPs) y 175 quistes radiculares (CRs), registrados en el Laboratorio de Patología de la Facultad de Odontología de Pernambuco FOP/UPE. Fueron utilizados para análisis inmunohistoquímica a través de la técnica de la estreptoavdina-biotina utilizando el anticuerpo anti CD68, para tanto, 79 casos, siendo 23 de granulomas periapicales y 56 de quistes radiculares. Los testes Chi-Cuadrado y Exacto de Fisher fueron empleados para verificar si existía asociación de las variables categóricas y clínicas, con 95% de confianza (p≤0,05). Resultados: Verificamos inmunoexpensión de la proteína CD68+ em 83% de los casos. Las lesiones de GP presentaron mayor número de células CD68+, (con puntaje variando de 3 a 4) con marcación, predominantemente, intensa. Verificamos diferencias significativas en relación a la cantidad de células CD68+ y la intensidad de esta marcación en las lesiones periapicales crónicas analizadas Conclusión: La presencia de células CD68+ en los tejidos periapicales pueden representar el desarrollo, manutención y/o severidad de la respuesta inmune mediada a la inflamación, siendo esa más intensa en GP.Objetivo: Analisar a expressão de macrófagos em granulomas periapicais e cistos radiculares. Metodologia: Foram selecionados 264 casos de lesões periapicais crônicas dos quais 89 eram granulomas periapicais (GPs) e 175 cistos radiculares (CRs), registrados no Laboratório de Patologia da Faculdade de Odontologia de Pernambuco FOP/UPE. Foram utilizados para análise imuno-histoquímica através da técnica da estreptoavdina-biotina utilizando o anticorpo anti CD68, para tanto, 79 casos, sendo 23 de granulomas periapicais e 56 de cistos radiculares. Os testes Qui-Quadrado e Exato de Fisher foram empregados para verificar se existia associação das variáveis categóricas e clínicas, com 95% de confiança (p≤0,05). Resultados: Verificamos imunoexpessão da proteína CD68+ em 83% dos casos. As lesões de GP apresentaram maior número de células CD68+, (com escore variando de 3 a 4) com marcação, predominantemente, intensa. Constatamos diferenças significativas em relação a quantidade de células CD68+ e a intensidade desta marcação nas lesões periapicais crônicas analisadas Conclusão: A presença de células CD68+ nos tecidos periapicais podem representar o desenvolvimento, manutenção e/ou severidade da resposta imune mediada à inflamação, sendo essa mais intensa em GP.Research, Society and Development2021-06-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1662210.33448/rsd-v10i7.16622Research, Society and Development; Vol. 10 No. 7; e35310716622Research, Society and Development; Vol. 10 Núm. 7; e35310716622Research, Society and Development; v. 10 n. 7; e353107166222525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/16622/14879Copyright (c) 2021 Zilda Betânia Barbosa Medeiros de Farias; Jade Souza Cavalcante; Anne Caroline de Lima; Emanuel Savio de Souza Andrade; Márcia Maria Fonseca da Silveira; Ana Paula Veras Sobralhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFarias, Zilda Betânia Barbosa Medeiros de Cavalcante, Jade SouzaLima, Anne Caroline deAndrade, Emanuel Savio de Souza Silveira, Márcia Maria Fonseca da Sobral, Ana Paula Veras 2021-07-18T21:07:03Zoai:ojs.pkp.sfu.ca:article/16622Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:37:07.415430Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Immunohistochemical expression of macrophages in chronic periapical lesions
Expresión inmunohistoquímica de macrófagos en lesiones periapicales crónicas
Expressão imunohistoquímica de macrófagos em lesões periapicais crônicas
title Immunohistochemical expression of macrophages in chronic periapical lesions
spellingShingle Immunohistochemical expression of macrophages in chronic periapical lesions
Farias, Zilda Betânia Barbosa Medeiros de
Granuloma periapical
Cisto radicular
Imuno-histoquímica
Macrófagos CD68.
Granuloma periapical
Quiste radicular
Inmunohistoquímica
Macrófagos CD68.
Periapical granuloma
Radicular cyst
Immunohistochemistry
CD68 macrophage.
title_short Immunohistochemical expression of macrophages in chronic periapical lesions
title_full Immunohistochemical expression of macrophages in chronic periapical lesions
title_fullStr Immunohistochemical expression of macrophages in chronic periapical lesions
title_full_unstemmed Immunohistochemical expression of macrophages in chronic periapical lesions
title_sort Immunohistochemical expression of macrophages in chronic periapical lesions
author Farias, Zilda Betânia Barbosa Medeiros de
author_facet Farias, Zilda Betânia Barbosa Medeiros de
Cavalcante, Jade Souza
Lima, Anne Caroline de
Andrade, Emanuel Savio de Souza
Silveira, Márcia Maria Fonseca da
Sobral, Ana Paula Veras
author_role author
author2 Cavalcante, Jade Souza
Lima, Anne Caroline de
Andrade, Emanuel Savio de Souza
Silveira, Márcia Maria Fonseca da
Sobral, Ana Paula Veras
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Farias, Zilda Betânia Barbosa Medeiros de
Cavalcante, Jade Souza
Lima, Anne Caroline de
Andrade, Emanuel Savio de Souza
Silveira, Márcia Maria Fonseca da
Sobral, Ana Paula Veras
dc.subject.por.fl_str_mv Granuloma periapical
Cisto radicular
Imuno-histoquímica
Macrófagos CD68.
Granuloma periapical
Quiste radicular
Inmunohistoquímica
Macrófagos CD68.
Periapical granuloma
Radicular cyst
Immunohistochemistry
CD68 macrophage.
topic Granuloma periapical
Cisto radicular
Imuno-histoquímica
Macrófagos CD68.
Granuloma periapical
Quiste radicular
Inmunohistoquímica
Macrófagos CD68.
Periapical granuloma
Radicular cyst
Immunohistochemistry
CD68 macrophage.
description Objective: To analyze the expression of macrophages in periapical granulomas (PGs) and radicular cysts (RCs). Methodology: We selected 264 cases of chronic periapical lesions stored at the Laboratory of Pathology, Dental School of Pernambuco (FOP)/UPE, including 89 PGs and 175 RCs. Seventy-nine cases, 23 PGs and 56 RCs, were submitted to immunohistochemical analysis by the streptavidin-biotin method using anti-CD68 antibody. The chi-square and Fisher’s exact tests were used to determine the existence of associations with categorical and clinical variables, adopting a 95% confidence level (p≤0.05). Results: We observed immunoexpression of CD68 in 83% of cases. The number of CD68+ cells (score 3 or 4) was higher in PGs, with predominantly intense staining. Significant differences in the number of CD68+ cells and staining intensity were observed between the chronic periapical lesions analyzed. Conclusion: The presence of CD68+ cells in periapical tissues may indicate the development, maintenance and/or severity of the inflammation-mediated immune response, which is more intense in PG.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-24
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/16622
10.33448/rsd-v10i7.16622
url https://rsdjournal.org/index.php/rsd/article/view/16622
identifier_str_mv 10.33448/rsd-v10i7.16622
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/16622/14879
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 7; e35310716622
Research, Society and Development; Vol. 10 Núm. 7; e35310716622
Research, Society and Development; v. 10 n. 7; e35310716622
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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