Comparison of four serological tests for the diagnosis of swine brucellosis

Detalhes bibliográficos
Autor(a) principal: Meirelles-Bartoli, Raphaella Barbosa
Data de Publicação: 2020
Outros Autores: Cruz, Carolina de Alvarenga, Moraes, Reiner Silveira de, Oliveira, Rafaela Assis, Paula, Eric Matheus Nascimento de, Sousa, Daniel Bartoli de, Assis, Nivaldo Aparecido de, Mathias, Luis Antonio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/4418
Resumo: The purpose of this article is to present results of a research in which the results of four serological tests were compared for the diagnosis of swine brucellosis in pigs from Brazilian herds in which the outbreak occurred in relation to other brucellosis-free.  Rose Bengal (RBT), complement fixation (CFT), agglutination plus 2-mercaptoethanol test (TAT+ME), and commercial fluorescence polarization assay (FPA) were used to test 333 sera (271 sows and 62 finishing pigs) from a Brucella suis infected swine herd and 1,100 swine sera from brucellosis free pigs taken at a slaughterhouse. Considering infected all the 271 sows from the outbreak, and interpreting results of the FPA according to the manufacturer directions, sensitivities observed were 95.94% for FPA, 94.83% for RBT, 93.73% for CFT, and 92.25% for TAT+ME. Considering infected all the pigs from the infected herd with a positive result in at least one test, sensitivities observed were 98.92% for RBT, 97.13% for CFT, 96.06% for FPA, and 94.98% for TAT+ME. Specificities of the tests were CFT and TAT+ME, 100%; FPA, 99.55%; and RBT 99.27%. Results indicated a good performance of all the tests, and the agreement among them was almost perfect.
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spelling Comparison of four serological tests for the diagnosis of swine brucellosisComparación de cuatro pruebas serológicas para el diagnóstico de brucelosis porcinaComparação de quatro testes sorológicos para o diagnóstico de brucelose suínaFluorescence polarizationComplement fixationRose BengalTube agglutinationBrucella suis.Polarização fluorescenteFixação complementoRosa bengalaTubo de aglutinaçãoBrucella suis.Polarización fluorescenteFijación del complementRosa de bengalaTubo de aglutinaciónBrucella suis.The purpose of this article is to present results of a research in which the results of four serological tests were compared for the diagnosis of swine brucellosis in pigs from Brazilian herds in which the outbreak occurred in relation to other brucellosis-free.  Rose Bengal (RBT), complement fixation (CFT), agglutination plus 2-mercaptoethanol test (TAT+ME), and commercial fluorescence polarization assay (FPA) were used to test 333 sera (271 sows and 62 finishing pigs) from a Brucella suis infected swine herd and 1,100 swine sera from brucellosis free pigs taken at a slaughterhouse. Considering infected all the 271 sows from the outbreak, and interpreting results of the FPA according to the manufacturer directions, sensitivities observed were 95.94% for FPA, 94.83% for RBT, 93.73% for CFT, and 92.25% for TAT+ME. Considering infected all the pigs from the infected herd with a positive result in at least one test, sensitivities observed were 98.92% for RBT, 97.13% for CFT, 96.06% for FPA, and 94.98% for TAT+ME. Specificities of the tests were CFT and TAT+ME, 100%; FPA, 99.55%; and RBT 99.27%. Results indicated a good performance of all the tests, and the agreement among them was almost perfect.El objetivo de este artículo es presentar los resultados de una investigación en la que se compararon los resultados de cuatro pruebas serológicas para el diagnóstico de brucelosis porcina en cerdos de rebaños brasileños en los que se produjo el brote en relación con otros sin brucelosis. Rosa Bengala (TRB), fijación de complemento (TFC), prueba de aglutinación con 2-mercaptoetanol (TA + 2-ME) y prueba de polarización fluorescente (TPF) se usaron para analizar 333 sueros (271 cerdas y 62 cerdos de finalización) de un rebaño con cerdos infectados con Brucella suis y 1.100 sueros de cerdos sin brucelosis recolectados en un matadero. Considerando todas las 271 cerdas del brote infectadas e interpretando los resultados de la TFP de acuerdo con las instrucciones del fabricante, las sensibilidades observadas fueron 95.94% para la TFP, 94.83% para TRB, 93.73% para TFC y 92 , 25% para TA + 2-ME. Los detalles de las pruebas fueron TFC y TA + 2-ME, 100%; TFP, 99,55%; y TRB 99.27%. Los resultados indicaron un buen desempeño de todas las pruebas y el acuerdo entre ellas fue casi perfecto.O objetivo do presente artigo é apresentar resultados de uma pesquisa na qual se fez a comparação de resultados de quarto testes sorologicos para o diagnóstico de brucelose suína em porcos de rebanhos brasileiros nos quais ocorreu o surto em relação a outros livres de brucelose. Rosa Bengala (TRB), Teste Fixação de Complemento (TFC), Teste de Aglutinação com 2-mercaptoetanol (TA+2-ME), e Teste de Polarização Fluorescente (TPF) foram utilizados para testar 333 soros (271 porcas e 62 suínos em terminação) de um rebanho com suínos infectados com Brucella suis e 1.100 soros de suínos livres de brucelose colhidos em um abatedouro. Considerando infectadas todas as 271 porcas do surto e interpretando os resultados do TFP de acordo com as instruções do fabricante, as sensibilidades observadas foram de 95,94% para o TFP, 94,83% para TRB, 93,73% para TFC e 92,25% para TA + 2-ME. As especificidades dos testes foram TFC e TA + 2-ME, 100%; TFP, 99,55%; e TRB 99,27%. Os resultados indicaram um bom desempenho de todos os testes e a concordância entre eles foi quase perfeita.Research, Society and Development2020-05-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/441810.33448/rsd-v9i7.4418Research, Society and Development; Vol. 9 No. 7; e493974418Research, Society and Development; Vol. 9 Núm. 7; e493974418Research, Society and Development; v. 9 n. 7; e4939744182525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/4418/3686Copyright (c) 2020 Raphaella Barbosa Meirelles-Bartoli, Carolina de Alvarenga Cruz, Reiner Silveira de Moraes, Rafaela Assis Oliveira, Daniel Bartoli de Sousa, Nivaldo Aparecido de Assis, Luis Antonio Mathiasinfo:eu-repo/semantics/openAccessMeirelles-Bartoli, Raphaella BarbosaCruz, Carolina de AlvarengaMoraes, Reiner Silveira deOliveira, Rafaela AssisPaula, Eric Matheus Nascimento deSousa, Daniel Bartoli deAssis, Nivaldo Aparecido deMathias, Luis Antonio2020-08-20T18:05:03Zoai:ojs.pkp.sfu.ca:article/4418Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:28:16.872831Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Comparison of four serological tests for the diagnosis of swine brucellosis
Comparación de cuatro pruebas serológicas para el diagnóstico de brucelosis porcina
Comparação de quatro testes sorológicos para o diagnóstico de brucelose suína
title Comparison of four serological tests for the diagnosis of swine brucellosis
spellingShingle Comparison of four serological tests for the diagnosis of swine brucellosis
Meirelles-Bartoli, Raphaella Barbosa
Fluorescence polarization
Complement fixation
Rose Bengal
Tube agglutination
Brucella suis.
Polarização fluorescente
Fixação complemento
Rosa bengala
Tubo de aglutinação
Brucella suis.
Polarización fluorescente
Fijación del complement
Rosa de bengala
Tubo de aglutinación
Brucella suis.
title_short Comparison of four serological tests for the diagnosis of swine brucellosis
title_full Comparison of four serological tests for the diagnosis of swine brucellosis
title_fullStr Comparison of four serological tests for the diagnosis of swine brucellosis
title_full_unstemmed Comparison of four serological tests for the diagnosis of swine brucellosis
title_sort Comparison of four serological tests for the diagnosis of swine brucellosis
author Meirelles-Bartoli, Raphaella Barbosa
author_facet Meirelles-Bartoli, Raphaella Barbosa
Cruz, Carolina de Alvarenga
Moraes, Reiner Silveira de
Oliveira, Rafaela Assis
Paula, Eric Matheus Nascimento de
Sousa, Daniel Bartoli de
Assis, Nivaldo Aparecido de
Mathias, Luis Antonio
author_role author
author2 Cruz, Carolina de Alvarenga
Moraes, Reiner Silveira de
Oliveira, Rafaela Assis
Paula, Eric Matheus Nascimento de
Sousa, Daniel Bartoli de
Assis, Nivaldo Aparecido de
Mathias, Luis Antonio
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Meirelles-Bartoli, Raphaella Barbosa
Cruz, Carolina de Alvarenga
Moraes, Reiner Silveira de
Oliveira, Rafaela Assis
Paula, Eric Matheus Nascimento de
Sousa, Daniel Bartoli de
Assis, Nivaldo Aparecido de
Mathias, Luis Antonio
dc.subject.por.fl_str_mv Fluorescence polarization
Complement fixation
Rose Bengal
Tube agglutination
Brucella suis.
Polarização fluorescente
Fixação complemento
Rosa bengala
Tubo de aglutinação
Brucella suis.
Polarización fluorescente
Fijación del complement
Rosa de bengala
Tubo de aglutinación
Brucella suis.
topic Fluorescence polarization
Complement fixation
Rose Bengal
Tube agglutination
Brucella suis.
Polarização fluorescente
Fixação complemento
Rosa bengala
Tubo de aglutinação
Brucella suis.
Polarización fluorescente
Fijación del complement
Rosa de bengala
Tubo de aglutinación
Brucella suis.
description The purpose of this article is to present results of a research in which the results of four serological tests were compared for the diagnosis of swine brucellosis in pigs from Brazilian herds in which the outbreak occurred in relation to other brucellosis-free.  Rose Bengal (RBT), complement fixation (CFT), agglutination plus 2-mercaptoethanol test (TAT+ME), and commercial fluorescence polarization assay (FPA) were used to test 333 sera (271 sows and 62 finishing pigs) from a Brucella suis infected swine herd and 1,100 swine sera from brucellosis free pigs taken at a slaughterhouse. Considering infected all the 271 sows from the outbreak, and interpreting results of the FPA according to the manufacturer directions, sensitivities observed were 95.94% for FPA, 94.83% for RBT, 93.73% for CFT, and 92.25% for TAT+ME. Considering infected all the pigs from the infected herd with a positive result in at least one test, sensitivities observed were 98.92% for RBT, 97.13% for CFT, 96.06% for FPA, and 94.98% for TAT+ME. Specificities of the tests were CFT and TAT+ME, 100%; FPA, 99.55%; and RBT 99.27%. Results indicated a good performance of all the tests, and the agreement among them was almost perfect.
publishDate 2020
dc.date.none.fl_str_mv 2020-05-23
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/4418
10.33448/rsd-v9i7.4418
url https://rsdjournal.org/index.php/rsd/article/view/4418
identifier_str_mv 10.33448/rsd-v9i7.4418
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/4418/3686
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 9 No. 7; e493974418
Research, Society and Development; Vol. 9 Núm. 7; e493974418
Research, Society and Development; v. 9 n. 7; e493974418
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052736224624640

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