Chemical study and antibacterial activity of the hydromethanolic extract of soil fungus Thichoderma sp FSF12

• Autor(a) principal: Cordeiro, Jorgeffson da Silva
• Data de Publicação: 2022
• Outros Autores: Pinheiro, Eduardo Antônio Abreu, Siqueira, José Edson de Sousa, Bitencourt, Heriberto Rodrigues, Marinho, Patrícia Santana Barbosa, Marinho, Andrey Moacir do Rosario
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Research, Society and Development
• Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/30852
• topic: Trichoderma; Actividad antimicrobiana; Hongos endofíticos; Sustancias.; Trichoderma; Antimicrobial activity; Endophytic fungi; Substances.; Trichoderma; Atividade antimicrobiana; Fungos endofíticos; Substâncias.

Bacterial resistance to antibiotics is a serious public health problem, which can lead to a simple limited infection or even death. The search for new compounds and / or extracts that can help fight these infections must be constantly renewed as a strategy to fight bacterial resistance. The objective of this work was to obtain bioactive substances. Thus, the fungus Trichoderma sp FSF12 was initially grown in cereal medium (rice) and after 28 days of growth the hydromethanolic extract was obtained. The extract was tested using the 96-well plate microdilution method against Gram-positive and Gram-negative bacteria and showed good activity. Then, the hydromethanolic extract had its chemical profile determined by HPLC-DAD, which indicated the presence of substances from different classes of secondary metabolites. The extract was fractionated by column chromatography and led to the isolation of the compounds polyunsaturated fatty acid (1), 5'-inosyl (2), tyrosol (3), harzialactone A (4) and 2-anhydromevalonic (5), which had their structures determined by 1D and 2D Nuclear Magnetic Resonance. The results obtained show the importance of searching for new antibacterial agents.