Production of mycelial biomass and lignocellulolytic enzymes of Pleurotus spp. in liquid culture medium
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/11406 |
Resumo: | Pleurotus spp. are fungi capable of degrading lignocellulose material due to the excretion of enzymes with different industrial applications. This study aimed to investigate the influence of culture media on the production of mycelial biomass and the activity of laccase, cellulases, and xylanase of Pleurotus spp. Pleurotus citrinopileatus U16-23, P. djamor U16-20, U16-25, and U16-28, P. eryngii U16-30, P. ostreatus U16-22, and P. pulmonarius U16-21 were grown for 12 days at 28 ºC in malt extract medium (EM-20 g L-1), defined medium (MD glucose 10 g L-1 and yeast extract 2 g L-1), or in sugarcane bagasse (BC) medium equal to MD, but replacing glucose with bagasse (50 g L-1). The greatest growth occurred in the medium EM and P. djamor U16-25 was the strain that generated the greatest biomass (7.5 ± 0.1 g L-1). Most strains showed higher laccase activity in the MD medium and the greatests activities were P. djamor U16-25 (22243 ± 745 U L-1), and P. pulmonarius (20924 ± 46 U L-1). P. citrinopileatus and P. djamor U16-25 did not produce cellulases, but P. djamor U16-20 and P. eryngii produced the greatest cellulase activities in EM and MD media. All strains produced xylanase, and the highest activities were observed in the EM medium and P. djamor U16-28 (67154 ± 1597 U L-1) was the best producer. Pleurotus djamor U16-25 excelled as a producer of laccase and cellulase-free xylanase, revealing the potential for application in the paper and cellulose industry. |
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Production of mycelial biomass and lignocellulolytic enzymes of Pleurotus spp. in liquid culture mediumProducción de biomasa micelial y enzimas lignocelulolíticas de Pleurotus spp. en medio de cultivo líquidoProdução de biomassa micelial e enzimas lignocelulolíticas de Pleurotus spp. em meio de cultivo líquidoBasidiomycotaCelulaseCogumelo-ostraLacaseMeio de cultivoXilanase.BasidiomycotaCellulaseCulture mediumLaccaseOyster mushroomXylanase.BasidiomycotaCelulasaLacasaMedios de cultivoSeta de ostraXilanasa.Pleurotus spp. are fungi capable of degrading lignocellulose material due to the excretion of enzymes with different industrial applications. This study aimed to investigate the influence of culture media on the production of mycelial biomass and the activity of laccase, cellulases, and xylanase of Pleurotus spp. Pleurotus citrinopileatus U16-23, P. djamor U16-20, U16-25, and U16-28, P. eryngii U16-30, P. ostreatus U16-22, and P. pulmonarius U16-21 were grown for 12 days at 28 ºC in malt extract medium (EM-20 g L-1), defined medium (MD glucose 10 g L-1 and yeast extract 2 g L-1), or in sugarcane bagasse (BC) medium equal to MD, but replacing glucose with bagasse (50 g L-1). The greatest growth occurred in the medium EM and P. djamor U16-25 was the strain that generated the greatest biomass (7.5 ± 0.1 g L-1). Most strains showed higher laccase activity in the MD medium and the greatests activities were P. djamor U16-25 (22243 ± 745 U L-1), and P. pulmonarius (20924 ± 46 U L-1). P. citrinopileatus and P. djamor U16-25 did not produce cellulases, but P. djamor U16-20 and P. eryngii produced the greatest cellulase activities in EM and MD media. All strains produced xylanase, and the highest activities were observed in the EM medium and P. djamor U16-28 (67154 ± 1597 U L-1) was the best producer. Pleurotus djamor U16-25 excelled as a producer of laccase and cellulase-free xylanase, revealing the potential for application in the paper and cellulose industry.Pleurotus spp. son hongos capaces de degradar la lignocelulosa debido a la excreción de enzimas con diferentes aplicaciones industriales. Este estudio tuvo como objetivo investigar la influencia de los medios de cultivo en la producción de biomasa micelial y la actividad de lacasa, celulasas y xilanasa de Pleurotus spp. Pleurotus citrinopileatus U16-23, P. djamor U16-20, U16-25 y U16-28, P. eryngii U16-30, P. ostreatus U16-22 y P. pulmonarius U16-21 se cultivaron durante 12 días a 28 ºC en medio extracto de malta (EM-20 g L-1), medio definido (MD glucosa 10 g L-1 y extracto de levadura 2 g L-1), o en medio de bagazo de caña de azúcar (BC) igual a MD, pero en sustitución de glucosa con bagazo (50 g L-1). El mayor crecimiento se presentó en el medio EM y P. djamor U16-25 fue la cepa que generó mayor biomasa (7.5 ± 0.1 g L-1). La mayoría de las cepas mostraron mayor actividad lacasa en el medio MD y las mayores actividades fueron P. djamor U16-25 (22243 ± 745 U L-1) y P. pulmonarius (20924 ± 46 U L-1). P. citrinopileatus y P. djamor U16-25 no produjeron celulasas, pero P. djamor U16-20 y P. eryngii produjeron las mayores actividades de celulasa en los medios EM y MD. Todas las cepas produjeron xilanasa, y las mayores actividades se observaron en el medio EM y P. djamor U16-28 (67154 ± 1597 U L-1) fue el mejor productor. Pleurotus djamor U16-25 se destacó como productor de de lacasa y xilanasa libre de celulasa, revelando el potencial de aplicación en la industria del papel y la celulosa.Pleurotus spp. são fungos capazes de degradar a lignocelulose graças à excreção de enzimas com diferentes aplicações industriais. O objetivo deste estudo foi avaliar a influência dos meios de cultivo na produção de biomassa micelial e na atividade de lacase, celulases e xilanase de Pleurotus spp. Pleurotus citrinopileatus U16-23, P. djamor U16-20, U16-25 e U16-28, P. eryngii U16-30, P. ostreatus U16-22 e P. pulmonarius U16-21 foram cultivados por 12 dias a 28 ºC em meio extrato de malte (EM-20 g L-1), meio definido (MD glicose 10 g L-1 e extrato de levedura 2 g L-1), ou em meio bagaço de cana-de-açúcar (BC) igual ao MD, mas substituindo glicose por bagaço (50 g L-1). O maior crescimento ocorreu no meio EM e P. djamor U16-25 a cepa que gerou mais biomassa (7,5 ± 0,1 g L-1). A maioria das cepas apresentou maior atividade de lacase no meio MD e as atividades maiores foram de P. djamor U16-25 (22243 ± 745 U L-1) e P. pulmonarius (20924 ± 46 U L-1). P. citrinopileatus e P. djamor U16-25 não produziram celulases, mas P. djamor U16-20 e P. eryngii produziram as maiores atividades de celulase nos meios EM e MD. Todas as cepas produziram xilanase em e as maiores atividades foram observadas no meio EM e P. djamor U16-28 (67154 ± 1597 U L-1) foi o maior produtor. Pleurotus djamor U16-25 se destacou como produtor de lacase e de xilanase livre de celulase revelando potencial para aplicação na indústria de papel e celulose.Research, Society and Development2021-01-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1140610.33448/rsd-v10i1.11406Research, Society and Development; Vol. 10 No. 1; e6810111406Research, Society and Development; Vol. 10 Núm. 1; e6810111406Research, Society and Development; v. 10 n. 1; e68101114062525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/11406/10261Copyright (c) 2021 Nelma Lopes Araújo; Katielle Vieira Avelino; Marisangela Isabel Wietzikoski Halabura; Renan Alberto Marim; Adma Soraia Serea Kassem; Thiago Teodoro Santana; Giani Andrea Linde Colauto; Nelson Barros Colauto; Juliana Silveira do Vallehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAraújo, Nelma LopesAvelino, Katielle VieiraHalabura, Marisangela Isabel WietzikoskiMarim, Renan AlbertoKassem, Adma Soraia SereaSantana, Thiago TeodoroColauto, Giani Andrea LindeColauto, Nelson BarrosValle, Juliana Silveira do2021-02-20T21:19:23Zoai:ojs.pkp.sfu.ca:article/11406Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:33:14.587951Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Production of mycelial biomass and lignocellulolytic enzymes of Pleurotus spp. in liquid culture medium Producción de biomasa micelial y enzimas lignocelulolíticas de Pleurotus spp. en medio de cultivo líquido Produção de biomassa micelial e enzimas lignocelulolíticas de Pleurotus spp. em meio de cultivo líquido |
title |
Production of mycelial biomass and lignocellulolytic enzymes of Pleurotus spp. in liquid culture medium |
spellingShingle |
Production of mycelial biomass and lignocellulolytic enzymes of Pleurotus spp. in liquid culture medium Araújo, Nelma Lopes Basidiomycota Celulase Cogumelo-ostra Lacase Meio de cultivo Xilanase. Basidiomycota Cellulase Culture medium Laccase Oyster mushroom Xylanase. Basidiomycota Celulasa Lacasa Medios de cultivo Seta de ostra Xilanasa. |
title_short |
Production of mycelial biomass and lignocellulolytic enzymes of Pleurotus spp. in liquid culture medium |
title_full |
Production of mycelial biomass and lignocellulolytic enzymes of Pleurotus spp. in liquid culture medium |
title_fullStr |
Production of mycelial biomass and lignocellulolytic enzymes of Pleurotus spp. in liquid culture medium |
title_full_unstemmed |
Production of mycelial biomass and lignocellulolytic enzymes of Pleurotus spp. in liquid culture medium |
title_sort |
Production of mycelial biomass and lignocellulolytic enzymes of Pleurotus spp. in liquid culture medium |
author |
Araújo, Nelma Lopes |
author_facet |
Araújo, Nelma Lopes Avelino, Katielle Vieira Halabura, Marisangela Isabel Wietzikoski Marim, Renan Alberto Kassem, Adma Soraia Serea Santana, Thiago Teodoro Colauto, Giani Andrea Linde Colauto, Nelson Barros Valle, Juliana Silveira do |
author_role |
author |
author2 |
Avelino, Katielle Vieira Halabura, Marisangela Isabel Wietzikoski Marim, Renan Alberto Kassem, Adma Soraia Serea Santana, Thiago Teodoro Colauto, Giani Andrea Linde Colauto, Nelson Barros Valle, Juliana Silveira do |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Araújo, Nelma Lopes Avelino, Katielle Vieira Halabura, Marisangela Isabel Wietzikoski Marim, Renan Alberto Kassem, Adma Soraia Serea Santana, Thiago Teodoro Colauto, Giani Andrea Linde Colauto, Nelson Barros Valle, Juliana Silveira do |
dc.subject.por.fl_str_mv |
Basidiomycota Celulase Cogumelo-ostra Lacase Meio de cultivo Xilanase. Basidiomycota Cellulase Culture medium Laccase Oyster mushroom Xylanase. Basidiomycota Celulasa Lacasa Medios de cultivo Seta de ostra Xilanasa. |
topic |
Basidiomycota Celulase Cogumelo-ostra Lacase Meio de cultivo Xilanase. Basidiomycota Cellulase Culture medium Laccase Oyster mushroom Xylanase. Basidiomycota Celulasa Lacasa Medios de cultivo Seta de ostra Xilanasa. |
description |
Pleurotus spp. are fungi capable of degrading lignocellulose material due to the excretion of enzymes with different industrial applications. This study aimed to investigate the influence of culture media on the production of mycelial biomass and the activity of laccase, cellulases, and xylanase of Pleurotus spp. Pleurotus citrinopileatus U16-23, P. djamor U16-20, U16-25, and U16-28, P. eryngii U16-30, P. ostreatus U16-22, and P. pulmonarius U16-21 were grown for 12 days at 28 ºC in malt extract medium (EM-20 g L-1), defined medium (MD glucose 10 g L-1 and yeast extract 2 g L-1), or in sugarcane bagasse (BC) medium equal to MD, but replacing glucose with bagasse (50 g L-1). The greatest growth occurred in the medium EM and P. djamor U16-25 was the strain that generated the greatest biomass (7.5 ± 0.1 g L-1). Most strains showed higher laccase activity in the MD medium and the greatests activities were P. djamor U16-25 (22243 ± 745 U L-1), and P. pulmonarius (20924 ± 46 U L-1). P. citrinopileatus and P. djamor U16-25 did not produce cellulases, but P. djamor U16-20 and P. eryngii produced the greatest cellulase activities in EM and MD media. All strains produced xylanase, and the highest activities were observed in the EM medium and P. djamor U16-28 (67154 ± 1597 U L-1) was the best producer. Pleurotus djamor U16-25 excelled as a producer of laccase and cellulase-free xylanase, revealing the potential for application in the paper and cellulose industry. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-03 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/11406 10.33448/rsd-v10i1.11406 |
url |
https://rsdjournal.org/index.php/rsd/article/view/11406 |
identifier_str_mv |
10.33448/rsd-v10i1.11406 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/11406/10261 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 1; e6810111406 Research, Society and Development; Vol. 10 Núm. 1; e6810111406 Research, Society and Development; v. 10 n. 1; e6810111406 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
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Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052782754136064 |