Immunological evaluation of the crevicular fluid in patients with gingivitis, periodontitis, and peri-implantitis: a 1-year cross-sectional study

• Autor(a) principal: Faria, Juliana Barbosa de
• Data de Publicação: 2021
• Outros Autores: Furtado, Taíssa Cássia de Souza, Assunção, Thaís Soares Farnesi de, Abdalla, Douglas Reis, Andrade, Fabiane Minin, Bertoldo, Bárbara Bellocchio, Amaral, Eleonora de Paula, Rodrigues, Denise Bertulucci Rocha, Rodrigues Júnior, Virmondes, Pereira, Sanivia Aparecida de Lima
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Research, Society and Development
• Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/20756
• topic: Citocinas; Doenças periodontais; Líquido do sulco gengival; Peri-implantite.; Citoquinas; Enfermedades periodontales; Líquido del surco gingival; Periimplantitis.; Cytokines; Periodontal diseases; Gingival crevicular fluid; Peri-implantitis.

Objective:  To assess the levels of IL-12, IL-15, IL-18, and IL-32 in the gingival crevicular fluid (GCF) and peri-implant crevicular fluid (PICF) in patients with gingivitis, periodontitis, and peri-implantitis before and one year after implant installation. Material and Methods: Forty-nine samples of GCF and PICF were collected from March 2018 to March 2019. The patients were classified: patients with gingivitis (n=7), patients with periodontitis (n=14), patients with peri-implantitis (n=4) and healthy patients (n=24). The crevicular fluid from the 49 patients was collected before implant installation (n=8) and one year after implant placement (n=8). The Enzyme-Linked Immunosorbent Assay (ELISA) was used to evaluate the levels of cytokines in crevicular fluid. Results: Patients with gingivitis, periodontitis, and peri-implantitis showed higher concentrations of IL-12, IL-15, IL-18, and IL-32 when compared with control group. In general, the levels of IL-12 and IL-15 increased when compared to the moments before and after implant installation. There was also an increase in the concentration of IL-18 in the control group volunteers after implant installation. Conclusion: The results and methodology of this study showed that there was no difference in the synthesis of IL-12, IL-15, IL-18, and IL-32 in healthy individuals or in those with periodontal injuries. However, there was an increase in the cytokines IL-12, IL-15, and IL-18 one year after implant installation, which would be increasing the inflammatory activity in peri-implantitis.