Diagnosis of babesia caballi in embryo donor mares and recipients by chain reaction technique polymerase

Detalhes bibliográficos
Autor(a) principal: Abreu , Juliana Freitas de
Data de Publicação: 2022
Outros Autores: Silva, Roberta de Araújo, Ferreira , Murilo Moura, Barra , Elem Cristina Macedo, Santos , Cintia Luana Pinheiro, Lima , Moises Moreira, Ribeiro , Haroldo Francisco Lobato, Barbosa , Elizabeth Machado, Ramos , Priscilla do Carmo de Azevedo, Rolim Filho , Sebastião Tavares, Casseb , Alexandre Rosário, Silva Filho , Ednaldo
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/24521
Resumo: Equine piroplasmosis is a disease transmitted by ticks infected by the protozoa Babesia caballi known as Babesiosis or Teileriosis. Clinical manifestations range from fever, anemia, apathy, to acute conditions where the animal becomes a reservoir of this microorganism causing reproductive losses in mares. The aim of this study was to identify the DNA sequences of pathogens and diagnose equine piroplasmosis by conventional PCR technique. 2 mL of blood were collected from 50 equine females of the Brasileiro de Hipismo (HB), Undefined Breed (SRD) and Quarter Horse breeds, of different ages, belonging to stud farms in the Northeast region of Brazil. The animals were divided into 2 groups, 9 animals in the donor group and 41 animals in the recipient group. The blood was sent to the molecular biology laboratory to be used in the detection of Babesia spp. Of the 50 equine females tested, none were positive for the protozoan Babesia spp. Result of good sanitary management and control of ectoparasites in donor and recipient mares.
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spelling Diagnosis of babesia caballi in embryo donor mares and recipients by chain reaction technique polymerase Diagnóstico de babesia spp. em yeguas donantes de embriones y destinatarias mediante técnica de reacción em cadena polimerasaDiagnóstico de babesia caballi em éguas doadoras e receptoras de embrião pela técnica de reação em cadeia polimeraseBabesia sppCaballosPCR.Babesia sppHorsesPCR.Babesia sppEquinosPCR.Equine piroplasmosis is a disease transmitted by ticks infected by the protozoa Babesia caballi known as Babesiosis or Teileriosis. Clinical manifestations range from fever, anemia, apathy, to acute conditions where the animal becomes a reservoir of this microorganism causing reproductive losses in mares. The aim of this study was to identify the DNA sequences of pathogens and diagnose equine piroplasmosis by conventional PCR technique. 2 mL of blood were collected from 50 equine females of the Brasileiro de Hipismo (HB), Undefined Breed (SRD) and Quarter Horse breeds, of different ages, belonging to stud farms in the Northeast region of Brazil. The animals were divided into 2 groups, 9 animals in the donor group and 41 animals in the recipient group. The blood was sent to the molecular biology laboratory to be used in the detection of Babesia spp. Of the 50 equine females tested, none were positive for the protozoan Babesia spp. Result of good sanitary management and control of ectoparasites in donor and recipient mares.La piroplasmosis equina es una enfermedad transmitida por garrapatas infectadas por los protozoos Babesia caballi y Theileria equi, conocida como Babesiosis o Theileriose. Las manifestaciones clínicas van desde fiebre, anemia, apatía, hasta condiciones agudas donde el animal se convierte en reservorio de este microorganismo provocando pérdidas reproductivas en las yeguas. El objetivo de este estudio fue identificar las secuencias de ADN de los patógenos y diagnosticar la piroplasmosis equina mediante la técnica de PCR convencional. Se recolectaron 2 mL de sangre de 50 hembras equinas de las razas BH, SRD y cuarto de milla, de diferentes edades, pertenecientes a ganaderías ubicadas en el noreste de Brasil. Los animales se dividieron en 2 grupos, 9 animales en el grupo donante y 41 animales en el grupo receptor, y la sangre se envió al laboratorio de biología molecular para ser utilizada en la detección de babesia spp. De las 50 hembras equinas analizadas, ninguna resultó positiva para el protozoo Babesia spp. Resultado de un buen manejo sanitario y control de ectoparásitos de yeguas donantes y receptoras.A piroplasmose equina é uma doença transmitida por carrapatos infectados pelos protozoários Babesia caballi e Theileria equi, conhecida, respectivamente, como Babesiose ou Theileriose. As manifestações clínicas variam desde febre, anemia, apatia, até quadros agudos que podem resultar em morte ou perdas reprodutivas em éguas. O objetivo deste estudo foi identificar as sequências de DNA de Babesia caballi através da técnica de PCR convencional e diagnosticar a ocorrência de piroplasmose equina. Foram coletados 2 mL de sangue de 50 fêmeas equinas das raças Brasileiro de Hipismo (BH), Sem Raça Definida (SRD) e Quarto de Milha (QM), de idades variadas, pertencentes à Haras localizados no nordeste do Brasil. Os animais foram divididos em 2 grupos, sendo 9 animais do grupo das doadoras e 41 animais no grupo das receptoras, o sangue foi enviado ao laboratório de biologia molecular para ser utilizado para detecção de Babesia spp. Das 50 fêmeas equinas testadas nenhuma foi positiva para o protozoário Babesia spp. Resultado do bom manejo sanitário e controle de ectoparasitas de éguas doadoras e receptoras de embriões.Research, Society and Development2022-01-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2452110.33448/rsd-v11i1.24521Research, Society and Development; Vol. 11 No. 1; e15811124521Research, Society and Development; Vol. 11 Núm. 1; e15811124521Research, Society and Development; v. 11 n. 1; e158111245212525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/24521/21743Copyright (c) 2022 Juliana Freitas de Abreu ; Roberta de Araújo Silva; Murilo Moura Ferreira ; Elem Cristina Macedo Barra ; Cintia Luana Pinheiro Santos ; Moises Moreira Lima ; Haroldo Francisco Lobato Ribeiro ; Elizabeth Machado Barbosa ; Priscilla do Carmo de Azevedo Ramos ; Sebastião Tavares Rolim Filho ; Alexandre Rosário Casseb ; Ednaldo Silva Filho https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAbreu , Juliana Freitas de Silva, Roberta de Araújo Ferreira , Murilo Moura Barra , Elem Cristina Macedo Santos , Cintia Luana Pinheiro Lima , Moises Moreira Ribeiro , Haroldo Francisco LobatoBarbosa , Elizabeth MachadoRamos , Priscilla do Carmo de Azevedo Rolim Filho , Sebastião Tavares Casseb , Alexandre RosárioSilva Filho , Ednaldo2022-01-16T18:08:18Zoai:ojs.pkp.sfu.ca:article/24521Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:43:02.815926Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Diagnosis of babesia caballi in embryo donor mares and recipients by chain reaction technique polymerase
Diagnóstico de babesia spp. em yeguas donantes de embriones y destinatarias mediante técnica de reacción em cadena polimerasa
Diagnóstico de babesia caballi em éguas doadoras e receptoras de embrião pela técnica de reação em cadeia polimerase
title Diagnosis of babesia caballi in embryo donor mares and recipients by chain reaction technique polymerase
spellingShingle Diagnosis of babesia caballi in embryo donor mares and recipients by chain reaction technique polymerase
Abreu , Juliana Freitas de
Babesia spp
Caballos
PCR.
Babesia spp
Horses
PCR.
Babesia spp
Equinos
PCR.
title_short Diagnosis of babesia caballi in embryo donor mares and recipients by chain reaction technique polymerase
title_full Diagnosis of babesia caballi in embryo donor mares and recipients by chain reaction technique polymerase
title_fullStr Diagnosis of babesia caballi in embryo donor mares and recipients by chain reaction technique polymerase
title_full_unstemmed Diagnosis of babesia caballi in embryo donor mares and recipients by chain reaction technique polymerase
title_sort Diagnosis of babesia caballi in embryo donor mares and recipients by chain reaction technique polymerase
author Abreu , Juliana Freitas de
author_facet Abreu , Juliana Freitas de
Silva, Roberta de Araújo
Ferreira , Murilo Moura
Barra , Elem Cristina Macedo
Santos , Cintia Luana Pinheiro
Lima , Moises Moreira
Ribeiro , Haroldo Francisco Lobato
Barbosa , Elizabeth Machado
Ramos , Priscilla do Carmo de Azevedo
Rolim Filho , Sebastião Tavares
Casseb , Alexandre Rosário
Silva Filho , Ednaldo
author_role author
author2 Silva, Roberta de Araújo
Ferreira , Murilo Moura
Barra , Elem Cristina Macedo
Santos , Cintia Luana Pinheiro
Lima , Moises Moreira
Ribeiro , Haroldo Francisco Lobato
Barbosa , Elizabeth Machado
Ramos , Priscilla do Carmo de Azevedo
Rolim Filho , Sebastião Tavares
Casseb , Alexandre Rosário
Silva Filho , Ednaldo
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Abreu , Juliana Freitas de
Silva, Roberta de Araújo
Ferreira , Murilo Moura
Barra , Elem Cristina Macedo
Santos , Cintia Luana Pinheiro
Lima , Moises Moreira
Ribeiro , Haroldo Francisco Lobato
Barbosa , Elizabeth Machado
Ramos , Priscilla do Carmo de Azevedo
Rolim Filho , Sebastião Tavares
Casseb , Alexandre Rosário
Silva Filho , Ednaldo
dc.subject.por.fl_str_mv Babesia spp
Caballos
PCR.
Babesia spp
Horses
PCR.
Babesia spp
Equinos
PCR.
topic Babesia spp
Caballos
PCR.
Babesia spp
Horses
PCR.
Babesia spp
Equinos
PCR.
description Equine piroplasmosis is a disease transmitted by ticks infected by the protozoa Babesia caballi known as Babesiosis or Teileriosis. Clinical manifestations range from fever, anemia, apathy, to acute conditions where the animal becomes a reservoir of this microorganism causing reproductive losses in mares. The aim of this study was to identify the DNA sequences of pathogens and diagnose equine piroplasmosis by conventional PCR technique. 2 mL of blood were collected from 50 equine females of the Brasileiro de Hipismo (HB), Undefined Breed (SRD) and Quarter Horse breeds, of different ages, belonging to stud farms in the Northeast region of Brazil. The animals were divided into 2 groups, 9 animals in the donor group and 41 animals in the recipient group. The blood was sent to the molecular biology laboratory to be used in the detection of Babesia spp. Of the 50 equine females tested, none were positive for the protozoan Babesia spp. Result of good sanitary management and control of ectoparasites in donor and recipient mares.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/24521
10.33448/rsd-v11i1.24521
url https://rsdjournal.org/index.php/rsd/article/view/24521
identifier_str_mv 10.33448/rsd-v11i1.24521
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/24521/21743
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 1; e15811124521
Research, Society and Development; Vol. 11 Núm. 1; e15811124521
Research, Society and Development; v. 11 n. 1; e15811124521
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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