Diagnosis of babesia caballi in embryo donor mares and recipients by chain reaction technique polymerase
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/24521 |
Resumo: | Equine piroplasmosis is a disease transmitted by ticks infected by the protozoa Babesia caballi known as Babesiosis or Teileriosis. Clinical manifestations range from fever, anemia, apathy, to acute conditions where the animal becomes a reservoir of this microorganism causing reproductive losses in mares. The aim of this study was to identify the DNA sequences of pathogens and diagnose equine piroplasmosis by conventional PCR technique. 2 mL of blood were collected from 50 equine females of the Brasileiro de Hipismo (HB), Undefined Breed (SRD) and Quarter Horse breeds, of different ages, belonging to stud farms in the Northeast region of Brazil. The animals were divided into 2 groups, 9 animals in the donor group and 41 animals in the recipient group. The blood was sent to the molecular biology laboratory to be used in the detection of Babesia spp. Of the 50 equine females tested, none were positive for the protozoan Babesia spp. Result of good sanitary management and control of ectoparasites in donor and recipient mares. |
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Research, Society and Development |
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Diagnosis of babesia caballi in embryo donor mares and recipients by chain reaction technique polymerase Diagnóstico de babesia spp. em yeguas donantes de embriones y destinatarias mediante técnica de reacción em cadena polimerasaDiagnóstico de babesia caballi em éguas doadoras e receptoras de embrião pela técnica de reação em cadeia polimeraseBabesia sppCaballosPCR.Babesia sppHorsesPCR.Babesia sppEquinosPCR.Equine piroplasmosis is a disease transmitted by ticks infected by the protozoa Babesia caballi known as Babesiosis or Teileriosis. Clinical manifestations range from fever, anemia, apathy, to acute conditions where the animal becomes a reservoir of this microorganism causing reproductive losses in mares. The aim of this study was to identify the DNA sequences of pathogens and diagnose equine piroplasmosis by conventional PCR technique. 2 mL of blood were collected from 50 equine females of the Brasileiro de Hipismo (HB), Undefined Breed (SRD) and Quarter Horse breeds, of different ages, belonging to stud farms in the Northeast region of Brazil. The animals were divided into 2 groups, 9 animals in the donor group and 41 animals in the recipient group. The blood was sent to the molecular biology laboratory to be used in the detection of Babesia spp. Of the 50 equine females tested, none were positive for the protozoan Babesia spp. Result of good sanitary management and control of ectoparasites in donor and recipient mares.La piroplasmosis equina es una enfermedad transmitida por garrapatas infectadas por los protozoos Babesia caballi y Theileria equi, conocida como Babesiosis o Theileriose. Las manifestaciones clínicas van desde fiebre, anemia, apatía, hasta condiciones agudas donde el animal se convierte en reservorio de este microorganismo provocando pérdidas reproductivas en las yeguas. El objetivo de este estudio fue identificar las secuencias de ADN de los patógenos y diagnosticar la piroplasmosis equina mediante la técnica de PCR convencional. Se recolectaron 2 mL de sangre de 50 hembras equinas de las razas BH, SRD y cuarto de milla, de diferentes edades, pertenecientes a ganaderías ubicadas en el noreste de Brasil. Los animales se dividieron en 2 grupos, 9 animales en el grupo donante y 41 animales en el grupo receptor, y la sangre se envió al laboratorio de biología molecular para ser utilizada en la detección de babesia spp. De las 50 hembras equinas analizadas, ninguna resultó positiva para el protozoo Babesia spp. Resultado de un buen manejo sanitario y control de ectoparásitos de yeguas donantes y receptoras.A piroplasmose equina é uma doença transmitida por carrapatos infectados pelos protozoários Babesia caballi e Theileria equi, conhecida, respectivamente, como Babesiose ou Theileriose. As manifestações clínicas variam desde febre, anemia, apatia, até quadros agudos que podem resultar em morte ou perdas reprodutivas em éguas. O objetivo deste estudo foi identificar as sequências de DNA de Babesia caballi através da técnica de PCR convencional e diagnosticar a ocorrência de piroplasmose equina. Foram coletados 2 mL de sangue de 50 fêmeas equinas das raças Brasileiro de Hipismo (BH), Sem Raça Definida (SRD) e Quarto de Milha (QM), de idades variadas, pertencentes à Haras localizados no nordeste do Brasil. Os animais foram divididos em 2 grupos, sendo 9 animais do grupo das doadoras e 41 animais no grupo das receptoras, o sangue foi enviado ao laboratório de biologia molecular para ser utilizado para detecção de Babesia spp. Das 50 fêmeas equinas testadas nenhuma foi positiva para o protozoário Babesia spp. Resultado do bom manejo sanitário e controle de ectoparasitas de éguas doadoras e receptoras de embriões.Research, Society and Development2022-01-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2452110.33448/rsd-v11i1.24521Research, Society and Development; Vol. 11 No. 1; e15811124521Research, Society and Development; Vol. 11 Núm. 1; e15811124521Research, Society and Development; v. 11 n. 1; e158111245212525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/24521/21743Copyright (c) 2022 Juliana Freitas de Abreu ; Roberta de Araújo Silva; Murilo Moura Ferreira ; Elem Cristina Macedo Barra ; Cintia Luana Pinheiro Santos ; Moises Moreira Lima ; Haroldo Francisco Lobato Ribeiro ; Elizabeth Machado Barbosa ; Priscilla do Carmo de Azevedo Ramos ; Sebastião Tavares Rolim Filho ; Alexandre Rosário Casseb ; Ednaldo Silva Filho https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAbreu , Juliana Freitas de Silva, Roberta de Araújo Ferreira , Murilo Moura Barra , Elem Cristina Macedo Santos , Cintia Luana Pinheiro Lima , Moises Moreira Ribeiro , Haroldo Francisco LobatoBarbosa , Elizabeth MachadoRamos , Priscilla do Carmo de Azevedo Rolim Filho , Sebastião Tavares Casseb , Alexandre RosárioSilva Filho , Ednaldo2022-01-16T18:08:18Zoai:ojs.pkp.sfu.ca:article/24521Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:43:02.815926Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Diagnosis of babesia caballi in embryo donor mares and recipients by chain reaction technique polymerase Diagnóstico de babesia spp. em yeguas donantes de embriones y destinatarias mediante técnica de reacción em cadena polimerasa Diagnóstico de babesia caballi em éguas doadoras e receptoras de embrião pela técnica de reação em cadeia polimerase |
title |
Diagnosis of babesia caballi in embryo donor mares and recipients by chain reaction technique polymerase |
spellingShingle |
Diagnosis of babesia caballi in embryo donor mares and recipients by chain reaction technique polymerase Abreu , Juliana Freitas de Babesia spp Caballos PCR. Babesia spp Horses PCR. Babesia spp Equinos PCR. |
title_short |
Diagnosis of babesia caballi in embryo donor mares and recipients by chain reaction technique polymerase |
title_full |
Diagnosis of babesia caballi in embryo donor mares and recipients by chain reaction technique polymerase |
title_fullStr |
Diagnosis of babesia caballi in embryo donor mares and recipients by chain reaction technique polymerase |
title_full_unstemmed |
Diagnosis of babesia caballi in embryo donor mares and recipients by chain reaction technique polymerase |
title_sort |
Diagnosis of babesia caballi in embryo donor mares and recipients by chain reaction technique polymerase |
author |
Abreu , Juliana Freitas de |
author_facet |
Abreu , Juliana Freitas de Silva, Roberta de Araújo Ferreira , Murilo Moura Barra , Elem Cristina Macedo Santos , Cintia Luana Pinheiro Lima , Moises Moreira Ribeiro , Haroldo Francisco Lobato Barbosa , Elizabeth Machado Ramos , Priscilla do Carmo de Azevedo Rolim Filho , Sebastião Tavares Casseb , Alexandre Rosário Silva Filho , Ednaldo |
author_role |
author |
author2 |
Silva, Roberta de Araújo Ferreira , Murilo Moura Barra , Elem Cristina Macedo Santos , Cintia Luana Pinheiro Lima , Moises Moreira Ribeiro , Haroldo Francisco Lobato Barbosa , Elizabeth Machado Ramos , Priscilla do Carmo de Azevedo Rolim Filho , Sebastião Tavares Casseb , Alexandre Rosário Silva Filho , Ednaldo |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Abreu , Juliana Freitas de Silva, Roberta de Araújo Ferreira , Murilo Moura Barra , Elem Cristina Macedo Santos , Cintia Luana Pinheiro Lima , Moises Moreira Ribeiro , Haroldo Francisco Lobato Barbosa , Elizabeth Machado Ramos , Priscilla do Carmo de Azevedo Rolim Filho , Sebastião Tavares Casseb , Alexandre Rosário Silva Filho , Ednaldo |
dc.subject.por.fl_str_mv |
Babesia spp Caballos PCR. Babesia spp Horses PCR. Babesia spp Equinos PCR. |
topic |
Babesia spp Caballos PCR. Babesia spp Horses PCR. Babesia spp Equinos PCR. |
description |
Equine piroplasmosis is a disease transmitted by ticks infected by the protozoa Babesia caballi known as Babesiosis or Teileriosis. Clinical manifestations range from fever, anemia, apathy, to acute conditions where the animal becomes a reservoir of this microorganism causing reproductive losses in mares. The aim of this study was to identify the DNA sequences of pathogens and diagnose equine piroplasmosis by conventional PCR technique. 2 mL of blood were collected from 50 equine females of the Brasileiro de Hipismo (HB), Undefined Breed (SRD) and Quarter Horse breeds, of different ages, belonging to stud farms in the Northeast region of Brazil. The animals were divided into 2 groups, 9 animals in the donor group and 41 animals in the recipient group. The blood was sent to the molecular biology laboratory to be used in the detection of Babesia spp. Of the 50 equine females tested, none were positive for the protozoan Babesia spp. Result of good sanitary management and control of ectoparasites in donor and recipient mares. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/24521 10.33448/rsd-v11i1.24521 |
url |
https://rsdjournal.org/index.php/rsd/article/view/24521 |
identifier_str_mv |
10.33448/rsd-v11i1.24521 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/24521/21743 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 1; e15811124521 Research, Society and Development; Vol. 11 Núm. 1; e15811124521 Research, Society and Development; v. 11 n. 1; e15811124521 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052700300410880 |