Complexes of electrospun nanofibers and nucleic acids: A review

Detalhes bibliográficos
Autor(a) principal: Cavalcante, Edmo Henrique Martins
Data de Publicação: 2021
Outros Autores: Oliveira, Helinando Pequeno de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/14953
Resumo: The interaction between cells and polymeric nanostructures has been considered an important topic for biotechnology. The electrospun fibers present porous structures in the order of submicron with characteristics that mimic the fibrillar components of the natural extracellular matrix, favoring the effective local action of bioactive systems. Complexation of nucleic acids with these fibers and their use in functional recovery and tissue regeneration therapies have been considered alternatives to cell transplantation and inductive protein delivery systems. In customizing the interaction profile between these matrices and the genetic material, it is relevant to maintain the availability of their molecular types in effective concentrations in the microenvironment, with greater gene expression and longer time for therapeutic action. The development of an adequate balance between transfection efficiency and cell viability, different strategies are followed, such as the incorporation of polynucleotides in polymeric solutions or emulsions before the electrospinning process, or even the functionalization of nanofibers by modifying their surface. Because of this, this review aims to present the different methods of producing electrophilic nanofibers functionalized with nucleic acids and their applications in the health area.
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spelling Complexes of electrospun nanofibers and nucleic acids: A reviewComplejos de nanofibras y ácidos nucleicos electrofilizados: Una revisión Complexos de nanofibras eletrofiadas e ácidos nucleicos: Uma revisãoNanofibersPolynucleotidesFunctionalization.NanofibrasPolinucleótidosFuncionalización.NanofibrasPolinucleotídeosFuncionalização.The interaction between cells and polymeric nanostructures has been considered an important topic for biotechnology. The electrospun fibers present porous structures in the order of submicron with characteristics that mimic the fibrillar components of the natural extracellular matrix, favoring the effective local action of bioactive systems. Complexation of nucleic acids with these fibers and their use in functional recovery and tissue regeneration therapies have been considered alternatives to cell transplantation and inductive protein delivery systems. In customizing the interaction profile between these matrices and the genetic material, it is relevant to maintain the availability of their molecular types in effective concentrations in the microenvironment, with greater gene expression and longer time for therapeutic action. The development of an adequate balance between transfection efficiency and cell viability, different strategies are followed, such as the incorporation of polynucleotides in polymeric solutions or emulsions before the electrospinning process, or even the functionalization of nanofibers by modifying their surface. Because of this, this review aims to present the different methods of producing electrophilic nanofibers functionalized with nucleic acids and their applications in the health area.La interacción entre células y nanoestructuras poliméricas se ha considerado un tema importante para la biotecnología. Las fibras electrofilizadas tienen estructuras porosas del orden submicrónico con características que imitan a los componentes fibrilares de la matriz extracelular natural, favoreciendo la acción local efectiva de los sistemas bioactivos. La complejación de ácidos nucleicos con estas fibras y su uso en terapias de recuperación funcional y regeneración de tejidos pueden ser alternativas al trasplante celular y los sistemas de liberación de proteínas inductivas. Al personalizar el perfil de interacción entre estas matrices y el material genético, es relevante mantener la disponibilidad de sus tipos moleculares en concentraciones efectivas en el microambiente, con mayor expresión génica y mayor tiempo de acción terapéutica. Cuando se busca un equilibrio entre la eficiencia de transfección y la viabilidad celular, se siguen diferentes estrategias, como la incorporación de polinucleótidos en soluciones o emulsiones poliméricas antes del proceso de electrohilado, o incluso la funcionalización de nanofibras modificando su superficie. Por tanto, esta revisión tiene como objetivo presentar los diferentes métodos de producción de nanofibras electrofilizadas funcionalizadas con ácidos nucleicos y sus aplicaciones en el área de la salud.A interação entre células e nanoestruturas poliméricas vem sendo considerado um importante tema para a biotecnologia. As fibras eletrofiadas apresentam estruturas porosas na ordem de submícrons com características que mimetizam os componentes fibrilares da matriz extracelular natural, favorecendo a atuação local efetiva de sistemas bioativos. A complexação de ácidos nucleicos com essas fibras e sua utilização em terapias de recuperação funcional e regeneração tecidual podem ser alternativas ao transplante celular e aos sistemas de entrega de proteínas indutivas. Na customização do perfil de interação entre essas matrizes e o material genético, é relevante manter a disponibilidade de seus tipos moleculares em concentrações efetivas no microambiente, com maior expressão gênica e maior tempo de ação terapêutica. Ao se buscar equilíbrio entre eficiência de transfecção e a viabilidade celular, diferentes estratégias são seguidas, como a incorporação de polinucleotídeos em soluções poliméricas ou em emulsões antes do processo de eletrofiação, ou mesmo a funcionalização de nanofibras pela modificação de sua superfície. Diante disso, esta revisão tem por finalidade apresentar os diferentes métodos de produção de nanofibras eletrofiadas funcionalizadas com ácidos nucleicos e suas aplicações na área da saúde.Research, Society and Development2021-04-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1495310.33448/rsd-v10i5.14953Research, Society and Development; Vol. 10 No. 5; e19210514953Research, Society and Development; Vol. 10 Núm. 5; e19210514953Research, Society and Development; v. 10 n. 5; e192105149532525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/14953/13216Copyright (c) 2021 Edmo Henrique Martins Cavalcante; Helinando Pequeno de Oliveirahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCavalcante, Edmo Henrique Martins Oliveira, Helinando Pequeno de 2021-05-17T18:20:49Zoai:ojs.pkp.sfu.ca:article/14953Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:35:54.198407Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Complexes of electrospun nanofibers and nucleic acids: A review
Complejos de nanofibras y ácidos nucleicos electrofilizados: Una revisión
Complexos de nanofibras eletrofiadas e ácidos nucleicos: Uma revisão
title Complexes of electrospun nanofibers and nucleic acids: A review
spellingShingle Complexes of electrospun nanofibers and nucleic acids: A review
Cavalcante, Edmo Henrique Martins
Nanofibers
Polynucleotides
Functionalization.
Nanofibras
Polinucleótidos
Funcionalización.
Nanofibras
Polinucleotídeos
Funcionalização.
title_short Complexes of electrospun nanofibers and nucleic acids: A review
title_full Complexes of electrospun nanofibers and nucleic acids: A review
title_fullStr Complexes of electrospun nanofibers and nucleic acids: A review
title_full_unstemmed Complexes of electrospun nanofibers and nucleic acids: A review
title_sort Complexes of electrospun nanofibers and nucleic acids: A review
author Cavalcante, Edmo Henrique Martins
author_facet Cavalcante, Edmo Henrique Martins
Oliveira, Helinando Pequeno de
author_role author
author2 Oliveira, Helinando Pequeno de
author2_role author
dc.contributor.author.fl_str_mv Cavalcante, Edmo Henrique Martins
Oliveira, Helinando Pequeno de
dc.subject.por.fl_str_mv Nanofibers
Polynucleotides
Functionalization.
Nanofibras
Polinucleótidos
Funcionalización.
Nanofibras
Polinucleotídeos
Funcionalização.
topic Nanofibers
Polynucleotides
Functionalization.
Nanofibras
Polinucleótidos
Funcionalización.
Nanofibras
Polinucleotídeos
Funcionalização.
description The interaction between cells and polymeric nanostructures has been considered an important topic for biotechnology. The electrospun fibers present porous structures in the order of submicron with characteristics that mimic the fibrillar components of the natural extracellular matrix, favoring the effective local action of bioactive systems. Complexation of nucleic acids with these fibers and their use in functional recovery and tissue regeneration therapies have been considered alternatives to cell transplantation and inductive protein delivery systems. In customizing the interaction profile between these matrices and the genetic material, it is relevant to maintain the availability of their molecular types in effective concentrations in the microenvironment, with greater gene expression and longer time for therapeutic action. The development of an adequate balance between transfection efficiency and cell viability, different strategies are followed, such as the incorporation of polynucleotides in polymeric solutions or emulsions before the electrospinning process, or even the functionalization of nanofibers by modifying their surface. Because of this, this review aims to present the different methods of producing electrophilic nanofibers functionalized with nucleic acids and their applications in the health area.
publishDate 2021
dc.date.none.fl_str_mv 2021-04-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/14953
10.33448/rsd-v10i5.14953
url https://rsdjournal.org/index.php/rsd/article/view/14953
identifier_str_mv 10.33448/rsd-v10i5.14953
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/14953/13216
dc.rights.driver.fl_str_mv Copyright (c) 2021 Edmo Henrique Martins Cavalcante; Helinando Pequeno de Oliveira
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Edmo Henrique Martins Cavalcante; Helinando Pequeno de Oliveira
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 5; e19210514953
Research, Society and Development; Vol. 10 Núm. 5; e19210514953
Research, Society and Development; v. 10 n. 5; e19210514953
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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