In vitro maturation of domestic cat oocytes subjected to different incubation times
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/13074 |
Resumo: | The aim of this study was to evaluate the effect of three different incubation times on in vitro maturation of domestic cat oocytes. Thus, ovaries (n = 42) were submitted to slicing procedure and the oocytes recovered were classified; only good quality oocytes (Grade I and II) underwent in vitro maturation for three different periods (24 vs. 30 vs. 36 h) in supplemented TCM-99 medium. After, oocytes were evaluated for cumulus cell expansion and presence of the first polar body. After six replicates (7 ± 1,7 ovaries per replicate), a total of 334 viable oocytes were recovered. Differences (p <0.05) were observed regarding the percentage of oocytes presenting expansion of the cumulus cells, where higher values were observed in the group of oocytes incubated for 36 h (84.3%), when compared to 30 (73.4%) and 24 h (71.0%). Moreover, differences were also observed regarding the presence of the first polar body (24 h: 29.7%; 30 h: 58.2%; 36 h: 69.8%). We conclude that the incubation period influenced the maturation rates, indicating 36 h as the ideal period for the in vitro maturation of domestic cat oocytes in supplemented TCM-199 medium. |
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In vitro maturation of domestic cat oocytes subjected to different incubation timesMaduración in vitro de ovocitos de gato doméstico sometidos a diferentes tiempos de incubaciónMaturação in vitro de oócitos de gatos domésticos submetidos a diferentes tempos de incubaçãoDomestic felineFirst polar bodyMaturationMeiotic competence.Felino domesticoPrimer corpúsculo polarMaduraciónCompetencia meiótica.Felino domésticoPrimeiro corpúsculo polarMaturaçãoCompetência meiótica.The aim of this study was to evaluate the effect of three different incubation times on in vitro maturation of domestic cat oocytes. Thus, ovaries (n = 42) were submitted to slicing procedure and the oocytes recovered were classified; only good quality oocytes (Grade I and II) underwent in vitro maturation for three different periods (24 vs. 30 vs. 36 h) in supplemented TCM-99 medium. After, oocytes were evaluated for cumulus cell expansion and presence of the first polar body. After six replicates (7 ± 1,7 ovaries per replicate), a total of 334 viable oocytes were recovered. Differences (p <0.05) were observed regarding the percentage of oocytes presenting expansion of the cumulus cells, where higher values were observed in the group of oocytes incubated for 36 h (84.3%), when compared to 30 (73.4%) and 24 h (71.0%). Moreover, differences were also observed regarding the presence of the first polar body (24 h: 29.7%; 30 h: 58.2%; 36 h: 69.8%). We conclude that the incubation period influenced the maturation rates, indicating 36 h as the ideal period for the in vitro maturation of domestic cat oocytes in supplemented TCM-199 medium.El objetivo de este estudio fue evaluar el efecto de tres tiempos de incubación diferentes sobre la maduración in vitro de ovocitos de gatos domésticos. Así, los ovarios (n = 42) se sometieron al procedimiento de corte y se clasificaron los ovocitos recuperados; solo ovocitos de buena calidad (grados I y II) se sometieron a maduración in vitro durante tres períodos diferentes (24 frente a 30 frente a 36 h) en medio TCM-99 suplementado. Luego, se evaluaron los ovocitos para determinar la expansión de las células del cúmulo y la presencia del primer cuerpo polar. Después de seis repeticiones (7 ± 1,7 por repetición), se recuperó un total de 334 ovocitos viables. Se observaron diferencias (p <0,05) en relación al porcentaje de ovocitos que presentan expansión celular de cúmulos, donde se observaron valores más altos en el grupo de ovocitos incubados por 36 h (84,3%), comparado con 30 (73,4 %) y 24h (71,0%). También se observaron diferencias en relación a la presencia del primer cuerpo polar (24 h: 29,7%; 30 h: 58,2%; 36 h: 69,8%). Concluimos que el período de incubación influyó en las tasas de maduración, indicando 36 h como el período ideal para la maduración in vitro de ovocitos de gatos domésticos en medio TCM-199 suplementado.O objetivo deste estudo foi avaliar o efeito de três diferentes tempos de incubação na maturação in vitro de oócitos de gatos domésticos. Assim, os ovários (n = 42) foram submetidos ao procedimento de slicing e os ovócitos recuperados foram classificados; apenas oócitos de boa qualidade (grau I e II) foram submetidos à maturação in vitro por três períodos diferentes (24 vs. 30 vs. 36 h) em meio TCM-199 suplementado. Em seguida, os oócitos foram avaliados quanto à expansão celular do cumulus e a presença do primeiro corpo polar. Após seis replicatas (7 ± 1,7 por replicata), um total de 334 oócitos viáveis foram recuperados. Diferenças (p <0,05) foram observadas em relação à porcentagem de oócitos que apresentam expansão das células do cumulus, onde maiores valores foram observados no grupo de oócitos incubados por 36 h (84,3%), quando comparados a 30 (73,4%) e 24 h (71,0%). Também foram observadas diferenças em relação à presença do primeiro corpo polar (24 h: 29,7%; 30 h: 58,2%; 36 h: 69,8%). Concluímos que o período de incubação influenciou as taxas de maturação, indicando 36 h como o período ideal para a maturação in vitro de oócitos de gatos domésticos em meio TCM-199 suplementado.Research, Society and Development2021-03-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1307410.33448/rsd-v10i3.13074Research, Society and Development; Vol. 10 No. 3; e15710313074Research, Society and Development; Vol. 10 Núm. 3; e15710313074Research, Society and Development; v. 10 n. 3; e157103130742525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/13074/11845Copyright (c) 2021 Denilsa Pires Fernandes ; Fernanda Araujo dos Santos ; Luã Barbalho de Macêdo; Roberta Gonçalves Izzo; Brenna de Sousa Barbosa; Muriel Magda Lustosa Pimentel; Alexsandra Fernandes Pereira; Marcelo Barbosa Bezerrahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFernandes , Denilsa Pires Santos , Fernanda Araujo dos Macêdo, Luã Barbalho de Izzo, Roberta Gonçalves Barbosa, Brenna de Sousa Pimentel, Muriel Magda Lustosa Pereira, Alexsandra Fernandes Bezerra, Marcelo Barbosa 2021-03-28T12:03:35Zoai:ojs.pkp.sfu.ca:article/13074Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:34:30.088370Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
In vitro maturation of domestic cat oocytes subjected to different incubation times Maduración in vitro de ovocitos de gato doméstico sometidos a diferentes tiempos de incubación Maturação in vitro de oócitos de gatos domésticos submetidos a diferentes tempos de incubação |
| title |
In vitro maturation of domestic cat oocytes subjected to different incubation times |
| spellingShingle |
In vitro maturation of domestic cat oocytes subjected to different incubation times Fernandes , Denilsa Pires Domestic feline First polar body Maturation Meiotic competence. Felino domestico Primer corpúsculo polar Maduración Competencia meiótica. Felino doméstico Primeiro corpúsculo polar Maturação Competência meiótica. |
| title_short |
In vitro maturation of domestic cat oocytes subjected to different incubation times |
| title_full |
In vitro maturation of domestic cat oocytes subjected to different incubation times |
| title_fullStr |
In vitro maturation of domestic cat oocytes subjected to different incubation times |
| title_full_unstemmed |
In vitro maturation of domestic cat oocytes subjected to different incubation times |
| title_sort |
In vitro maturation of domestic cat oocytes subjected to different incubation times |
| author |
Fernandes , Denilsa Pires |
| author_facet |
Fernandes , Denilsa Pires Santos , Fernanda Araujo dos Macêdo, Luã Barbalho de Izzo, Roberta Gonçalves Barbosa, Brenna de Sousa Pimentel, Muriel Magda Lustosa Pereira, Alexsandra Fernandes Bezerra, Marcelo Barbosa |
| author_role |
author |
| author2 |
Santos , Fernanda Araujo dos Macêdo, Luã Barbalho de Izzo, Roberta Gonçalves Barbosa, Brenna de Sousa Pimentel, Muriel Magda Lustosa Pereira, Alexsandra Fernandes Bezerra, Marcelo Barbosa |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Fernandes , Denilsa Pires Santos , Fernanda Araujo dos Macêdo, Luã Barbalho de Izzo, Roberta Gonçalves Barbosa, Brenna de Sousa Pimentel, Muriel Magda Lustosa Pereira, Alexsandra Fernandes Bezerra, Marcelo Barbosa |
| dc.subject.por.fl_str_mv |
Domestic feline First polar body Maturation Meiotic competence. Felino domestico Primer corpúsculo polar Maduración Competencia meiótica. Felino doméstico Primeiro corpúsculo polar Maturação Competência meiótica. |
| topic |
Domestic feline First polar body Maturation Meiotic competence. Felino domestico Primer corpúsculo polar Maduración Competencia meiótica. Felino doméstico Primeiro corpúsculo polar Maturação Competência meiótica. |
| description |
The aim of this study was to evaluate the effect of three different incubation times on in vitro maturation of domestic cat oocytes. Thus, ovaries (n = 42) were submitted to slicing procedure and the oocytes recovered were classified; only good quality oocytes (Grade I and II) underwent in vitro maturation for three different periods (24 vs. 30 vs. 36 h) in supplemented TCM-99 medium. After, oocytes were evaluated for cumulus cell expansion and presence of the first polar body. After six replicates (7 ± 1,7 ovaries per replicate), a total of 334 viable oocytes were recovered. Differences (p <0.05) were observed regarding the percentage of oocytes presenting expansion of the cumulus cells, where higher values were observed in the group of oocytes incubated for 36 h (84.3%), when compared to 30 (73.4%) and 24 h (71.0%). Moreover, differences were also observed regarding the presence of the first polar body (24 h: 29.7%; 30 h: 58.2%; 36 h: 69.8%). We conclude that the incubation period influenced the maturation rates, indicating 36 h as the ideal period for the in vitro maturation of domestic cat oocytes in supplemented TCM-199 medium. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-03-10 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://rsdjournal.org/index.php/rsd/article/view/13074 10.33448/rsd-v10i3.13074 |
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https://rsdjournal.org/index.php/rsd/article/view/13074 |
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10.33448/rsd-v10i3.13074 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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https://rsdjournal.org/index.php/rsd/article/view/13074/11845 |
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https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development; Vol. 10 No. 3; e15710313074 Research, Society and Development; Vol. 10 Núm. 3; e15710313074 Research, Society and Development; v. 10 n. 3; e15710313074 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Universidade Federal de Itajubá (UNIFEI) |
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UNIFEI |
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UNIFEI |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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rsd.articles@gmail.com |
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1797052824643698688 |