Evaluation of the in vitro cytogenotoxic profile of the silver(I) metal complex with furosemide (Ag-FSE)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/34986 |
Resumo: | The aim of the present study was to evaluate the cytotoxic potential of a new complex of silver(I) with furosemide (Ag-FSE) over a panel of tumor and non-tumor human cells, as well as its genotoxicological safety. According to the results obtained, cell line derived from colorectal adenocarcinoma (Caco-2) was the most sensitive to the Ag-FSE complex, and among the non-tumor cells was the non-transforming fibroblasts known as GM07492A, which stimulated the characterization of cytotoxic properties against these cells. In addition to interfering with the cellular metabolic profile (evidenced by the resazurin method), Ag-FSE alters the integrity of cell membranes (results obtained by the Trypan blue assay) regardless of the cell line evaluated. However, interference with clonogenic capacity is dependent on the cell being tested; the cytotoxic activity evaluated by the clonogenic survival assay was evidenced against Caco-2 cells, but not against GM07492A cells. Furthermore, Ag-FSE did not induce an increase in the extent of DNA damage detectable by the comet assay, nor in the frequency of micronuclei compared to the negative control, demonstrating the absence of genotoxicity under the experimental conditions used. The data of the present study provide information on various biological aspects of the Ag-FSE complex and help in the generation of new drug candidates with potential to impact the health area, targeting cancer treatment. |
id |
UNIFEI_be8e31142665d63563279418107175b7 |
---|---|
oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/34986 |
network_acronym_str |
UNIFEI |
network_name_str |
Research, Society and Development |
repository_id_str |
|
spelling |
Evaluation of the in vitro cytogenotoxic profile of the silver(I) metal complex with furosemide (Ag-FSE)Avaliação do perfil citogenotóxico in vitro do complexo metálico de prata(I) com furosemida (Ag-FSE)Evaluación del perfil citogenotóxico in vitro del complejo metálico de prata (I) con furosemida (Ag-FSE)Inestabilidad genômicaComplejo de plataToxicogenéticaCitotoxicidadMutagenicidad.Instabilidade genômicaComplexo de prataToxicogenéticaCitotoxicidadeMutagenicidade.Genomic instabilitySilver complexToxicogeneticsCytotoxicityMutagenicity.The aim of the present study was to evaluate the cytotoxic potential of a new complex of silver(I) with furosemide (Ag-FSE) over a panel of tumor and non-tumor human cells, as well as its genotoxicological safety. According to the results obtained, cell line derived from colorectal adenocarcinoma (Caco-2) was the most sensitive to the Ag-FSE complex, and among the non-tumor cells was the non-transforming fibroblasts known as GM07492A, which stimulated the characterization of cytotoxic properties against these cells. In addition to interfering with the cellular metabolic profile (evidenced by the resazurin method), Ag-FSE alters the integrity of cell membranes (results obtained by the Trypan blue assay) regardless of the cell line evaluated. However, interference with clonogenic capacity is dependent on the cell being tested; the cytotoxic activity evaluated by the clonogenic survival assay was evidenced against Caco-2 cells, but not against GM07492A cells. Furthermore, Ag-FSE did not induce an increase in the extent of DNA damage detectable by the comet assay, nor in the frequency of micronuclei compared to the negative control, demonstrating the absence of genotoxicity under the experimental conditions used. The data of the present study provide information on various biological aspects of the Ag-FSE complex and help in the generation of new drug candidates with potential to impact the health area, targeting cancer treatment.El objetivo del presente estudio fue evaluar el potencial citotóxico de un nuevo complejo de plata(I) con furosemida (Ag-FSE) en un panel de células tumorales y no tumorales humanas, así como su seguridad genotoxicológica. De acuerdo con los resultados obtenidos, la línea celular derivada del adenocarcinoma colorrectal (Caco-2) fue la más sensible al complejo Ag-FSE, y entre las células no tumorales se encontraban los fibroblastos no transformantes conocidos como GM07492A, lo que estimuló la caracterización. de las propiedades citotóxicas contra estas células. Además de interferir con el perfil metabólico celular (evidenciado por el método de resazurina), Ag-FSE altera la integridad de las membranas celulares (resultados obtenidos por el ensayo de azul de tripano) independientemente del linaje celular evaluado. Sin embargo, la interferencia con la capacidad clonogénica depende de la célula que se esté analizando; la actividad citotóxica evaluada por el ensayo de supervivencia clonogénica se evidenció frente a las células Caco-2, pero no frente a las células GM07492A. Además, Ag-FSE no indujo un aumento en la extensión del daño del ADN detectable por el ensayo Comet, ni en la frecuencia de micronúcleos en relación con el control negativo, lo que demuestra la ausencia de genotoxicidad en las condiciones experimentales utilizadas. Los datos del presente estudio brindan información sobre varios aspectos biológicos del complejo Ag-FSE y ayudan en la generación de nuevos candidatos a fármacos con potencial impacto en el área de la salud, con miras al tratamiento del cáncer.O objetivo do presente estudo foi avaliar o potencial citotóxico de um novo complexo de prata(I) com furosemida (Ag-FSE) sobre um painel de células humanas tumorais e não tumorais, bem como sua segurança genotoxicológica. De acordo com os resultados obtidos, a linhagem celular derivada do adenocarcinoma colorretal (Caco-2) foi a mais sensível ao complexo Ag-FSE, e dentre as células não tumorais foram fibroblastos não transformantes conhecidos como GM07492A, o que estimulou a caracterização das propriedades citotóxicas contra essas células. Além de interferir no perfil metabólico celular (evidenciado pelo método da resazurina), Ag-FSE altera a integridade das membranas celulares (resultados obtidos pelo ensaio do azul de Trypan) independentemente da linhagem celular avaliada. No entanto, a interferência na capacidade clonogênica depende da célula que está sendo testada; a atividade citotóxica avaliada pelo ensaio de sobrevivência clonogênica foi evidenciada contra células Caco-2, mas não contra células GM07492A. Além disso, Ag-FSE não induziu aumento na extensão do dano ao DNA detectável pelo ensaio Cometa, nem na frequência de micronúcleos em relação ao controle negativo, demonstrando ausência de genotoxicidade nas condições experimentais utilizadas. Os dados do presente estudo fornecem informações sobre diversos aspectos biológicos do complexo Ag-FSE e auxiliam na geração de novos candidatos a fármacos com potencial de impacto na área da saúde, visando o tratamento do câncer.Research, Society and Development2022-09-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3498610.33448/rsd-v11i13.34986Research, Society and Development; Vol. 11 No. 13; e21111334986Research, Society and Development; Vol. 11 Núm. 13; e21111334986Research, Society and Development; v. 11 n. 13; e211113349862525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/34986/29434Copyright (c) 2022 Gabriela de Cássia Gasparoti; Pietra Stefany da Silva Gomes; Nadia Andrade Aleixo; Juliana Gonella Fornielles da Silva; Igor Henrique Cerqueira; Wilton Rogério Lustri; Flávia Aparecida Resendehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessGasparoti, Gabriela de CássiaGomes, Pietra Stefany da Silva Aleixo, Nadia Andrade Silva, Juliana Gonella Fornielles da Cerqueira, Igor Henrique Lustri, Wilton Rogério Resende, Flávia Aparecida2022-10-17T13:43:46Zoai:ojs.pkp.sfu.ca:article/34986Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:50:04.149643Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Evaluation of the in vitro cytogenotoxic profile of the silver(I) metal complex with furosemide (Ag-FSE) Avaliação do perfil citogenotóxico in vitro do complexo metálico de prata(I) com furosemida (Ag-FSE) Evaluación del perfil citogenotóxico in vitro del complejo metálico de prata (I) con furosemida (Ag-FSE) |
title |
Evaluation of the in vitro cytogenotoxic profile of the silver(I) metal complex with furosemide (Ag-FSE) |
spellingShingle |
Evaluation of the in vitro cytogenotoxic profile of the silver(I) metal complex with furosemide (Ag-FSE) Gasparoti, Gabriela de Cássia Inestabilidad genômica Complejo de plata Toxicogenética Citotoxicidad Mutagenicidad. Instabilidade genômica Complexo de prata Toxicogenética Citotoxicidade Mutagenicidade. Genomic instability Silver complex Toxicogenetics Cytotoxicity Mutagenicity. |
title_short |
Evaluation of the in vitro cytogenotoxic profile of the silver(I) metal complex with furosemide (Ag-FSE) |
title_full |
Evaluation of the in vitro cytogenotoxic profile of the silver(I) metal complex with furosemide (Ag-FSE) |
title_fullStr |
Evaluation of the in vitro cytogenotoxic profile of the silver(I) metal complex with furosemide (Ag-FSE) |
title_full_unstemmed |
Evaluation of the in vitro cytogenotoxic profile of the silver(I) metal complex with furosemide (Ag-FSE) |
title_sort |
Evaluation of the in vitro cytogenotoxic profile of the silver(I) metal complex with furosemide (Ag-FSE) |
author |
Gasparoti, Gabriela de Cássia |
author_facet |
Gasparoti, Gabriela de Cássia Gomes, Pietra Stefany da Silva Aleixo, Nadia Andrade Silva, Juliana Gonella Fornielles da Cerqueira, Igor Henrique Lustri, Wilton Rogério Resende, Flávia Aparecida |
author_role |
author |
author2 |
Gomes, Pietra Stefany da Silva Aleixo, Nadia Andrade Silva, Juliana Gonella Fornielles da Cerqueira, Igor Henrique Lustri, Wilton Rogério Resende, Flávia Aparecida |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Gasparoti, Gabriela de Cássia Gomes, Pietra Stefany da Silva Aleixo, Nadia Andrade Silva, Juliana Gonella Fornielles da Cerqueira, Igor Henrique Lustri, Wilton Rogério Resende, Flávia Aparecida |
dc.subject.por.fl_str_mv |
Inestabilidad genômica Complejo de plata Toxicogenética Citotoxicidad Mutagenicidad. Instabilidade genômica Complexo de prata Toxicogenética Citotoxicidade Mutagenicidade. Genomic instability Silver complex Toxicogenetics Cytotoxicity Mutagenicity. |
topic |
Inestabilidad genômica Complejo de plata Toxicogenética Citotoxicidad Mutagenicidad. Instabilidade genômica Complexo de prata Toxicogenética Citotoxicidade Mutagenicidade. Genomic instability Silver complex Toxicogenetics Cytotoxicity Mutagenicity. |
description |
The aim of the present study was to evaluate the cytotoxic potential of a new complex of silver(I) with furosemide (Ag-FSE) over a panel of tumor and non-tumor human cells, as well as its genotoxicological safety. According to the results obtained, cell line derived from colorectal adenocarcinoma (Caco-2) was the most sensitive to the Ag-FSE complex, and among the non-tumor cells was the non-transforming fibroblasts known as GM07492A, which stimulated the characterization of cytotoxic properties against these cells. In addition to interfering with the cellular metabolic profile (evidenced by the resazurin method), Ag-FSE alters the integrity of cell membranes (results obtained by the Trypan blue assay) regardless of the cell line evaluated. However, interference with clonogenic capacity is dependent on the cell being tested; the cytotoxic activity evaluated by the clonogenic survival assay was evidenced against Caco-2 cells, but not against GM07492A cells. Furthermore, Ag-FSE did not induce an increase in the extent of DNA damage detectable by the comet assay, nor in the frequency of micronuclei compared to the negative control, demonstrating the absence of genotoxicity under the experimental conditions used. The data of the present study provide information on various biological aspects of the Ag-FSE complex and help in the generation of new drug candidates with potential to impact the health area, targeting cancer treatment. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-09-26 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/34986 10.33448/rsd-v11i13.34986 |
url |
https://rsdjournal.org/index.php/rsd/article/view/34986 |
identifier_str_mv |
10.33448/rsd-v11i13.34986 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/34986/29434 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 13; e21111334986 Research, Society and Development; Vol. 11 Núm. 13; e21111334986 Research, Society and Development; v. 11 n. 13; e21111334986 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052723861913600 |