Vasorelaxation endothelium-independent of the ethyl acetate phase from aerial parts of Solanum paludosum Moric. envolves channels-calcium L-type blockade

Detalhes bibliográficos
Autor(a) principal: Silva, Joelmir Lucena Veiga da
Data de Publicação: 2021
Outros Autores: Cavalcante, Fabiana de Andrade, Silva, Tania Maria Sarmento da, Ramos, Alessandro Elery, Oliveira, Thárcia Kiara Beserra, Pereira, Schirley Cristina Almeida, Silva, Bagnólia Araújo da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/11845
Resumo: Previous results showed the ethyl acetate phase (SP-AcOEt), obtained from aerial parts of Solanum paludosum, relaxed the aorta isolated in the endothelium-dependent and -independent manner. The vasorelaxant effects of SP-AcOEt was not characterized on aorta rings endothelium-denuded, thus this work aimed to elucidate the mechanisms endothelium-independent vasorelaxation on rat isolated aorta. The aorta was isolated from Wistar rats and mounted in glass baths containing 6 mL of normal Krebs physiological solution with pH at 7.4. The preparation was maintained at 37ºC and bubbled continuously with a mixture of 95% O2 and 5% CO2. Aortic rings were maintained for 1 hour by a resting tension of 1g and next were contracted with phenylephrine after the sustained contraction ACh was added to access the integrity of the endothelium. SP-AcOEt relaxed pre-contracted aorta by KCl-30mM or -80mM in a similar manner, suggesting blockade CaV, but not channel-K+ participating. SP-AcOEt also inhibited the contraction induced by CaCl2 and relaxed pre-contracted aorta by (±)-BayK8644 (EC50 = 16.9±1.3 μg/mL), which confirms the involvement of L-type CaV blockade. SP-AcOEt presented vasorelaxation endothelium-independent that involves L-type CaV blockade.
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spelling Vasorelaxation endothelium-independent of the ethyl acetate phase from aerial parts of Solanum paludosum Moric. envolves channels-calcium L-type blockadeVasorrelajación independiente del endotelio de la fase de acetato de etilo obtenida de las partes aéreas de Solanum paludosum Moric. implica bloquear los canales de calcio de tipo LVasorrelaxamento independente de endotélio da fase acetato de etila obtida das partes aéreas de Solanum paludosum Moric. envolve bloqueio de canais de cálcio do tipo LPlanta medicinalMúsculo liso vascularSolanaceaeCanais de cálcio do tipo L.Medicinal plantVascular smooth muscleSolanaceaeL-type calcium channels.Planta medicinalMúsculo liso vascularSolanaceaeCanais de cálcio do tipo L.Previous results showed the ethyl acetate phase (SP-AcOEt), obtained from aerial parts of Solanum paludosum, relaxed the aorta isolated in the endothelium-dependent and -independent manner. The vasorelaxant effects of SP-AcOEt was not characterized on aorta rings endothelium-denuded, thus this work aimed to elucidate the mechanisms endothelium-independent vasorelaxation on rat isolated aorta. The aorta was isolated from Wistar rats and mounted in glass baths containing 6 mL of normal Krebs physiological solution with pH at 7.4. The preparation was maintained at 37ºC and bubbled continuously with a mixture of 95% O2 and 5% CO2. Aortic rings were maintained for 1 hour by a resting tension of 1g and next were contracted with phenylephrine after the sustained contraction ACh was added to access the integrity of the endothelium. SP-AcOEt relaxed pre-contracted aorta by KCl-30mM or -80mM in a similar manner, suggesting blockade CaV, but not channel-K+ participating. SP-AcOEt also inhibited the contraction induced by CaCl2 and relaxed pre-contracted aorta by (±)-BayK8644 (EC50 = 16.9±1.3 μg/mL), which confirms the involvement of L-type CaV blockade. SP-AcOEt presented vasorelaxation endothelium-independent that involves L-type CaV blockade.Resultados anteriores demostraron que la fase de acetato de etilo (SP-AcOEt), obtenida de las partes aéreas de Solanum paludosum, relajó la aorta aislada de forma dependiente e independiente del endotelio. Los efectos vasorrelajantes de SP-AcOEt no se caracterizaron en anillos aórticos sin endotelio, por lo que este trabajo tuvo como objetivo dilucidar los mecanismos de vasorrelajación independientes del endotelio en aorta aislada de rata. Las aortas se aislaron de ratas Wistar y se montaron en viales de vidrio para un órgano aislado que contenía 6 ml de solución salina de Krebs con pH 7,4. La preparación se mantuvo a 37ºC y se aireó continuamente con carbogen. Los anillos aórticos se mantuvieron estabilizados durante 1 h, bajo una tensión de reposo de 1 gy luego se precontrataron con Fen seguido de ACh, después de una contracción sostenida, para observar la integridad del endotelio funcional. La fase SP-AcOEt relajó la aorta precontraída con KCl-30 y KCl-80 mM de manera similar, lo que sugiere bloqueo de CaV, pero no la participación de canales de K+. SP-AcOEt también inhibió las contracciones inducidas por CaCl2, medio K+ y sin calcio, y relajó la aorta precontraída con (±)-BayK8644 (EC50 = 16,9±1,3 μg/mL), confirmando la afectación del bloqueo CaV tipo L. La fase SP-AcOEt mostró vasorrelajación independiente del endotelio que involucra bloqueo de CaV tipo L.Resultados anteriores mostraram que a fase acetato de etila (SP-AcOEt), obtida das partes aéreas de Solanum paludosum, relaxou a aorta isolada de maneira dependente e independente de endotélio. Os efeitos vasorrelaxantes de SP-AcOEt não foram caracterizados nos anéis de aorta sem endotélio, assim este trabalho objetivou elucidar os mecanismos vasorrelaxantes independentes de endotélio em aorta isolada de rato. As aortas foram isoladas de ratos Wistar e montadas em cubas de vidro para órgão isolado contendo 6 mL de solução fisiológica de Krebs com pH 7,4. A preparação era mantida a 37º C e aerada continuamente com carbogênio. Os anéis de aorta foram mantidos em estabilização por 1 h, sob tensão de repouso de 1 g, e depois foram pré-contraídos com fenilefrina seguido de ACh, após uma contração sustentada, para observar a integridade do endotélio funcional. A fase SP-AcOEt relaxou a aorta pré-contraída com KCl-30 e KCl-80mM de maneira similar, sugerindo o bloqueio de CaV, mas não a participação de canais-K+. SP-AcOEt também inibiu as contrações induzidas por CaCl2, meio alto K+ e sem cálcio, e relaxou a aorta pré-contraída com (±)-BayK8644 (CE50 = 16,9±1,3 μg/mL), confirmando o envolvimento do boqueio de CaV do tipo-L. A fase SP-AcOEt mostrou vasorrelaxamento independente de endotélio que envolve o bloqueio de CaV do tipo-L.Research, Society and Development2021-01-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1184510.33448/rsd-v10i1.11845Research, Society and Development; Vol. 10 No. 1; e31710111845Research, Society and Development; Vol. 10 Núm. 1; e31710111845Research, Society and Development; v. 10 n. 1; e317101118452525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/11845/10563Copyright (c) 2021 Joelmir Lucena Veiga da Silva; Fabiana de Andrade Cavalcante; Tania Maria Sarmento da Silva; Alessandro Elery Ramos; Thárcia Kiara Beserra Oliveira; Schirley Cristina Almeida Pereira; Bagnólia Araújo da Silvahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva, Joelmir Lucena Veiga daCavalcante, Fabiana de AndradeSilva, Tania Maria Sarmento daRamos, Alessandro EleryOliveira, Thárcia Kiara BeserraPereira, Schirley Cristina AlmeidaSilva, Bagnólia Araújo da 2021-02-20T21:19:23Zoai:ojs.pkp.sfu.ca:article/11845Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:33:34.245471Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Vasorelaxation endothelium-independent of the ethyl acetate phase from aerial parts of Solanum paludosum Moric. envolves channels-calcium L-type blockade
Vasorrelajación independiente del endotelio de la fase de acetato de etilo obtenida de las partes aéreas de Solanum paludosum Moric. implica bloquear los canales de calcio de tipo L
Vasorrelaxamento independente de endotélio da fase acetato de etila obtida das partes aéreas de Solanum paludosum Moric. envolve bloqueio de canais de cálcio do tipo L
title Vasorelaxation endothelium-independent of the ethyl acetate phase from aerial parts of Solanum paludosum Moric. envolves channels-calcium L-type blockade
spellingShingle Vasorelaxation endothelium-independent of the ethyl acetate phase from aerial parts of Solanum paludosum Moric. envolves channels-calcium L-type blockade
Silva, Joelmir Lucena Veiga da
Planta medicinal
Músculo liso vascular
Solanaceae
Canais de cálcio do tipo L.
Medicinal plant
Vascular smooth muscle
Solanaceae
L-type calcium channels.
Planta medicinal
Músculo liso vascular
Solanaceae
Canais de cálcio do tipo L.
title_short Vasorelaxation endothelium-independent of the ethyl acetate phase from aerial parts of Solanum paludosum Moric. envolves channels-calcium L-type blockade
title_full Vasorelaxation endothelium-independent of the ethyl acetate phase from aerial parts of Solanum paludosum Moric. envolves channels-calcium L-type blockade
title_fullStr Vasorelaxation endothelium-independent of the ethyl acetate phase from aerial parts of Solanum paludosum Moric. envolves channels-calcium L-type blockade
title_full_unstemmed Vasorelaxation endothelium-independent of the ethyl acetate phase from aerial parts of Solanum paludosum Moric. envolves channels-calcium L-type blockade
title_sort Vasorelaxation endothelium-independent of the ethyl acetate phase from aerial parts of Solanum paludosum Moric. envolves channels-calcium L-type blockade
author Silva, Joelmir Lucena Veiga da
author_facet Silva, Joelmir Lucena Veiga da
Cavalcante, Fabiana de Andrade
Silva, Tania Maria Sarmento da
Ramos, Alessandro Elery
Oliveira, Thárcia Kiara Beserra
Pereira, Schirley Cristina Almeida
Silva, Bagnólia Araújo da
author_role author
author2 Cavalcante, Fabiana de Andrade
Silva, Tania Maria Sarmento da
Ramos, Alessandro Elery
Oliveira, Thárcia Kiara Beserra
Pereira, Schirley Cristina Almeida
Silva, Bagnólia Araújo da
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Joelmir Lucena Veiga da
Cavalcante, Fabiana de Andrade
Silva, Tania Maria Sarmento da
Ramos, Alessandro Elery
Oliveira, Thárcia Kiara Beserra
Pereira, Schirley Cristina Almeida
Silva, Bagnólia Araújo da
dc.subject.por.fl_str_mv Planta medicinal
Músculo liso vascular
Solanaceae
Canais de cálcio do tipo L.
Medicinal plant
Vascular smooth muscle
Solanaceae
L-type calcium channels.
Planta medicinal
Músculo liso vascular
Solanaceae
Canais de cálcio do tipo L.
topic Planta medicinal
Músculo liso vascular
Solanaceae
Canais de cálcio do tipo L.
Medicinal plant
Vascular smooth muscle
Solanaceae
L-type calcium channels.
Planta medicinal
Músculo liso vascular
Solanaceae
Canais de cálcio do tipo L.
description Previous results showed the ethyl acetate phase (SP-AcOEt), obtained from aerial parts of Solanum paludosum, relaxed the aorta isolated in the endothelium-dependent and -independent manner. The vasorelaxant effects of SP-AcOEt was not characterized on aorta rings endothelium-denuded, thus this work aimed to elucidate the mechanisms endothelium-independent vasorelaxation on rat isolated aorta. The aorta was isolated from Wistar rats and mounted in glass baths containing 6 mL of normal Krebs physiological solution with pH at 7.4. The preparation was maintained at 37ºC and bubbled continuously with a mixture of 95% O2 and 5% CO2. Aortic rings were maintained for 1 hour by a resting tension of 1g and next were contracted with phenylephrine after the sustained contraction ACh was added to access the integrity of the endothelium. SP-AcOEt relaxed pre-contracted aorta by KCl-30mM or -80mM in a similar manner, suggesting blockade CaV, but not channel-K+ participating. SP-AcOEt also inhibited the contraction induced by CaCl2 and relaxed pre-contracted aorta by (±)-BayK8644 (EC50 = 16.9±1.3 μg/mL), which confirms the involvement of L-type CaV blockade. SP-AcOEt presented vasorelaxation endothelium-independent that involves L-type CaV blockade.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-17
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/11845
10.33448/rsd-v10i1.11845
url https://rsdjournal.org/index.php/rsd/article/view/11845
identifier_str_mv 10.33448/rsd-v10i1.11845
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/11845/10563
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 1; e31710111845
Research, Society and Development; Vol. 10 Núm. 1; e31710111845
Research, Society and Development; v. 10 n. 1; e31710111845
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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