Ethanolic extract of Caryocar brasiliense fruit peel promotes death and cell cycle control in canine osteosarcoma cells
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/9194 |
Resumo: | The Cerrado biome is a source of chemical molecules with great medicinal potential. The substances extracted from pequi, the fruit of Caryocar brasiliense, have antiparasitic, antioxidant, and antineoplastic properties. Canine osteosarcoma is a highly aggressive and metastatic bone tumor, not very responsive to current chemotherapy modalities. This study aimed to show the effects of the ethanol extract of pequi peel (EEPP) on canine osteosarcoma cells, in addition to suggesting a metabolic route that explains the action of the extract. D-17 cells were seeded and exposed to EEPP at concentrations of 0, 10, and 100 µg/mL. After treatment, the cell nuclei were marked with DAPI and quantified using fluorescence microscopy. The expression of proteins p53, Ki-67, Bcl-2, Akt, AMPK, and mTOR was analyzed using immunocytochemistry. By using DAPI, we found a reduction in the number of quantified nuclei, time, and dose-dependent. The labeling of p53, Ki-67, and Bcl-2, antibodies decreased in groups exposed to EEPP. The opposite was observed with Akt, AMPK, and mTOR antibodies, where the protein was not found in the control group but was expressed in groups exposed to EEPP. We suggest a possible metabolic route where EEPP promotes cell death and cell cycle control in D-17 cells. |
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Ethanolic extract of Caryocar brasiliense fruit peel promotes death and cell cycle control in canine osteosarcoma cells El extracto de etanol de la cáscara de la fruta Caryocar brasiliense promueve la muerte y el control del ciclo celular en las células del osteosarcoma caninoExtrato etanólico da casca de fruta de Caryocar brasiliense promove a morte e o controle do ciclo celular em células osteossarcoma caninasApoptoseAutofagiaCiclo de bloqueioMetabolismo celularCitoproteção.ApoptosisAutofagiaCiclo de bloqueoMetabolismo celularCitoprotección.ApoptosisAutophagyCycle blockCellular metabolismCytoprotection. The Cerrado biome is a source of chemical molecules with great medicinal potential. The substances extracted from pequi, the fruit of Caryocar brasiliense, have antiparasitic, antioxidant, and antineoplastic properties. Canine osteosarcoma is a highly aggressive and metastatic bone tumor, not very responsive to current chemotherapy modalities. This study aimed to show the effects of the ethanol extract of pequi peel (EEPP) on canine osteosarcoma cells, in addition to suggesting a metabolic route that explains the action of the extract. D-17 cells were seeded and exposed to EEPP at concentrations of 0, 10, and 100 µg/mL. After treatment, the cell nuclei were marked with DAPI and quantified using fluorescence microscopy. The expression of proteins p53, Ki-67, Bcl-2, Akt, AMPK, and mTOR was analyzed using immunocytochemistry. By using DAPI, we found a reduction in the number of quantified nuclei, time, and dose-dependent. The labeling of p53, Ki-67, and Bcl-2, antibodies decreased in groups exposed to EEPP. The opposite was observed with Akt, AMPK, and mTOR antibodies, where the protein was not found in the control group but was expressed in groups exposed to EEPP. We suggest a possible metabolic route where EEPP promotes cell death and cell cycle control in D-17 cells.El bioma del Cerrado es una fuente de moléculas químicas con gran potencial medicinal. Las sustancias extraídas del pequi, fruto del Caryocar brasiliense, tienen propiedades antiparasitarias, antioxidantes y antineoplásicas. El osteosarcoma canino es un tumor óseo metastásico y altamente agresivo, que no responde mucho a las modalidades de quimioterapia actuales. Este estudio tuvo como objetivo mostrar los efectos del extracto etanólico de piel de pequi (EEPP) sobre las células del osteosarcoma canino, además de sugerir una ruta metabólica que explica la acción del extracto. Se sembraron células D-17 y se expusieron a EEPP a concentraciones de 0, 10 y 100 µg / ml. Después del tratamiento, los núcleos celulares se marcaron con DAPI y se cuantificaron mediante microscopía de fluorescencia. La expresión de las proteínas p53, Ki-67, Bcl-2, Akt, AMPK y mTOR se analizó mediante inmunocitoquímica. Al usar DAPI, encontramos una reducción en el número de núcleos cuantificados, dependiente del tiempo y de la dosis. El marcaje de los anticuerpos p53, Ki-67 y Bcl-2 disminuyó en los grupos expuestos a EEPP. Lo contrario se observó con los anticuerpos Akt, AMPK y mTOR, donde la proteína no se encontró en el grupo control, sino que se expresó en los grupos expuestos a EEPP. Sugerimos una posible vía metabólica en la que EEPP promueve la muerte celular y el control del ciclo celular en las células D-17.O bioma Cerrado é fonte de moléculas químicas com grande potencial medicinal. As substâncias extraídas do pequi, fruto do Caryocar brasiliense, têm propriedades antiparasitárias, antioxidantes e antineoplásicas. O osteossarcoma canino é um tumor ósseo altamente agressivo e metastático, pouco responsivo às modalidades atuais de quimioterapia. Este estudo teve como objetivo mostrar os efeitos do extrato etanólico da casca de pequi (EEPP) sobre as células do osteossarcoma canino, além de sugerir uma rota metabólica que explique a ação do extrato. As células D-17 foram semeadas e expostas a EEPP nas concentrações de 0, 10 e 100 µg / mL. Após o tratamento, os núcleos celulares foram marcados com DAPI e quantificados por microscopia de fluorescência. A expressão das proteínas p53, Ki-67, Bcl-2, Akt, AMPK e mTOR foi analisada por imunocitoquímica. Ao usar o DAPI, encontramos uma redução no número de núcleos quantificados, tempo e dose-dependente. A marcação de anticorpos p53, Ki-67 e Bcl-2 diminuiu nos grupos expostos a EEPP. O oposto foi observado com os anticorpos Akt, AMPK e mTOR, onde a proteína não foi encontrada no grupo controle, mas foi expressa nos grupos expostos à EEPP. Nós sugerimos uma possível rota metabólica onde EEPP promove a morte celular e o controle do ciclo celular em células D-17.Research, Society and Development2020-10-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/919410.33448/rsd-v9i10.9194Research, Society and Development; Vol. 9 No. 10; e7299109194Research, Society and Development; Vol. 9 Núm. 10; e7299109194Research, Society and Development; v. 9 n. 10; e72991091942525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/9194/8135Copyright (c) 2020 Leandro Lopes Nepomuceno; Nayane Peixoto Soares; Juliana Carvalho de Almeida Borges; Vanessa de Souza Vieira; Dayane Kelly Sabec Pereira; Kleber Fernando Pereira; Vanessa de Sousa Cruz; Jorge Luís Ferreira; Emmanuel Arnhold; Eugênio Gonçalves de Araújohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessNepomuceno, Leandro Lopes Soares, Nayane Peixoto Borges, Juliana Carvalho de Almeida Vieira, Vanessa de SouzaPereira, Dayane Kelly SabecPereira, Kleber FernandoCruz, Vanessa de Sousa Ferreira, Jorge Luís Arnhold, Emmanuel Araújo, Eugênio Gonçalves de 2020-10-31T12:03:23Zoai:ojs.pkp.sfu.ca:article/9194Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:31:31.998623Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Ethanolic extract of Caryocar brasiliense fruit peel promotes death and cell cycle control in canine osteosarcoma cells El extracto de etanol de la cáscara de la fruta Caryocar brasiliense promueve la muerte y el control del ciclo celular en las células del osteosarcoma canino Extrato etanólico da casca de fruta de Caryocar brasiliense promove a morte e o controle do ciclo celular em células osteossarcoma caninas |
title |
Ethanolic extract of Caryocar brasiliense fruit peel promotes death and cell cycle control in canine osteosarcoma cells |
spellingShingle |
Ethanolic extract of Caryocar brasiliense fruit peel promotes death and cell cycle control in canine osteosarcoma cells Nepomuceno, Leandro Lopes Apoptose Autofagia Ciclo de bloqueio Metabolismo celular Citoproteção. Apoptosis Autofagia Ciclo de bloqueo Metabolismo celular Citoprotección. Apoptosis Autophagy Cycle block Cellular metabolism Cytoprotection. |
title_short |
Ethanolic extract of Caryocar brasiliense fruit peel promotes death and cell cycle control in canine osteosarcoma cells |
title_full |
Ethanolic extract of Caryocar brasiliense fruit peel promotes death and cell cycle control in canine osteosarcoma cells |
title_fullStr |
Ethanolic extract of Caryocar brasiliense fruit peel promotes death and cell cycle control in canine osteosarcoma cells |
title_full_unstemmed |
Ethanolic extract of Caryocar brasiliense fruit peel promotes death and cell cycle control in canine osteosarcoma cells |
title_sort |
Ethanolic extract of Caryocar brasiliense fruit peel promotes death and cell cycle control in canine osteosarcoma cells |
author |
Nepomuceno, Leandro Lopes |
author_facet |
Nepomuceno, Leandro Lopes Soares, Nayane Peixoto Borges, Juliana Carvalho de Almeida Vieira, Vanessa de Souza Pereira, Dayane Kelly Sabec Pereira, Kleber Fernando Cruz, Vanessa de Sousa Ferreira, Jorge Luís Arnhold, Emmanuel Araújo, Eugênio Gonçalves de |
author_role |
author |
author2 |
Soares, Nayane Peixoto Borges, Juliana Carvalho de Almeida Vieira, Vanessa de Souza Pereira, Dayane Kelly Sabec Pereira, Kleber Fernando Cruz, Vanessa de Sousa Ferreira, Jorge Luís Arnhold, Emmanuel Araújo, Eugênio Gonçalves de |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Nepomuceno, Leandro Lopes Soares, Nayane Peixoto Borges, Juliana Carvalho de Almeida Vieira, Vanessa de Souza Pereira, Dayane Kelly Sabec Pereira, Kleber Fernando Cruz, Vanessa de Sousa Ferreira, Jorge Luís Arnhold, Emmanuel Araújo, Eugênio Gonçalves de |
dc.subject.por.fl_str_mv |
Apoptose Autofagia Ciclo de bloqueio Metabolismo celular Citoproteção. Apoptosis Autofagia Ciclo de bloqueo Metabolismo celular Citoprotección. Apoptosis Autophagy Cycle block Cellular metabolism Cytoprotection. |
topic |
Apoptose Autofagia Ciclo de bloqueio Metabolismo celular Citoproteção. Apoptosis Autofagia Ciclo de bloqueo Metabolismo celular Citoprotección. Apoptosis Autophagy Cycle block Cellular metabolism Cytoprotection. |
description |
The Cerrado biome is a source of chemical molecules with great medicinal potential. The substances extracted from pequi, the fruit of Caryocar brasiliense, have antiparasitic, antioxidant, and antineoplastic properties. Canine osteosarcoma is a highly aggressive and metastatic bone tumor, not very responsive to current chemotherapy modalities. This study aimed to show the effects of the ethanol extract of pequi peel (EEPP) on canine osteosarcoma cells, in addition to suggesting a metabolic route that explains the action of the extract. D-17 cells were seeded and exposed to EEPP at concentrations of 0, 10, and 100 µg/mL. After treatment, the cell nuclei were marked with DAPI and quantified using fluorescence microscopy. The expression of proteins p53, Ki-67, Bcl-2, Akt, AMPK, and mTOR was analyzed using immunocytochemistry. By using DAPI, we found a reduction in the number of quantified nuclei, time, and dose-dependent. The labeling of p53, Ki-67, and Bcl-2, antibodies decreased in groups exposed to EEPP. The opposite was observed with Akt, AMPK, and mTOR antibodies, where the protein was not found in the control group but was expressed in groups exposed to EEPP. We suggest a possible metabolic route where EEPP promotes cell death and cell cycle control in D-17 cells. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-16 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/9194 10.33448/rsd-v9i10.9194 |
url |
https://rsdjournal.org/index.php/rsd/article/view/9194 |
identifier_str_mv |
10.33448/rsd-v9i10.9194 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/9194/8135 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 9 No. 10; e7299109194 Research, Society and Development; Vol. 9 Núm. 10; e7299109194 Research, Society and Development; v. 9 n. 10; e7299109194 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052823985192960 |