Isolation and partial characterization of the lipoxygenase gene in black pepper (Piper nigrum L)
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/32254 |
Resumo: | Black pepper is a spice that have an economic importance in the world by its wide application in industry and by medicinal properties. State of Pará is one of the largest producers of these spices, but the production of this species is committed by fusariosis, that causes root rot in plant. Study of molecular biology is important by support breeding improvement of black pepper. In this work we draw the primers Lox3R and Lox3F to amplify a larger fragment of the gene lipoxygenase (Lox) in black pepper. The amplified fragment using primers drawn was sequenced. The sequence isolated has 770 nucleotides that encode 258 amino acids. This sequence was characterized by comparison in biological databases and using computational programs. The analysis with BlastX showed that sequence isolated has high similarity with lipoxygenase proteins of Persea americana, Parasponia andersonii e Vitis vinífera. We verified that Lox of black pepper has the PLAT/ LH2 domain of plant lipoxygenase related proteins. Its description in black pepper is essential to clarify the molecular mechanisms of response of the plant to the Fungus and understand its paper in activation of the defense response, once the gene Lox is activated in plant to signal its defense in a possible attack against pathogen and may be precursors of metabolic regulators. |
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Isolation and partial characterization of the lipoxygenase gene in black pepper (Piper nigrum L)Aislamiento y caracterización parcial del gen de la lipoxigenasa en pimienta negra (Piper nigrum L)Isolamento e caracterização parcial do gene lipoxigenase em pimenta-do-reino (Piper nigrum L)Gene lipoxygenasePiper nigrum L.DefenseResponse.Gen de la lipoxigenasaPiper nigrum L.DefensaRespuesta.Gene lipoxigenasePiper nigrum L.DefesaResposta.Black pepper is a spice that have an economic importance in the world by its wide application in industry and by medicinal properties. State of Pará is one of the largest producers of these spices, but the production of this species is committed by fusariosis, that causes root rot in plant. Study of molecular biology is important by support breeding improvement of black pepper. In this work we draw the primers Lox3R and Lox3F to amplify a larger fragment of the gene lipoxygenase (Lox) in black pepper. The amplified fragment using primers drawn was sequenced. The sequence isolated has 770 nucleotides that encode 258 amino acids. This sequence was characterized by comparison in biological databases and using computational programs. The analysis with BlastX showed that sequence isolated has high similarity with lipoxygenase proteins of Persea americana, Parasponia andersonii e Vitis vinífera. We verified that Lox of black pepper has the PLAT/ LH2 domain of plant lipoxygenase related proteins. Its description in black pepper is essential to clarify the molecular mechanisms of response of the plant to the Fungus and understand its paper in activation of the defense response, once the gene Lox is activated in plant to signal its defense in a possible attack against pathogen and may be precursors of metabolic regulators.La pimienta negra es una especia que tiene importancia económica en el mundo por su extensa aplicación en la industria y sus propiedades medicinales. El estado de Pará es uno de los mayores productores de esta especia, sin embargo su producción se ve afectada por la fusariosis, que provoca la pudrición de la raíz de la planta. Los estudios de biología molecular son importantes para apoyar a los programas de mejoramiento genético en pimienta negra. En este trabajo fueron diseñados los primers Lox3R y Lox3f para amplificar un fragmento mayor del gen de la lipoxigenasa (Lox) en pimienta negra. El fragmento amplificado, empleando los primers diseñados, fue secuenciado. La secuencia aislada tiene alrededor de 770 nucleótidos que codifican 258 aminoácidos. Esta secuencia fue caracterizada por comparación, empleando bases de datos biológicos y programas informáticos. El análisis BlastX mostro, que la secuencia aislada presenta una gran similitud con las proteínas lipoxigenasas de Persea americana, Parasponia andersonii y Vitis vinífera. Se observó que Lox de pimienta negra tiene el dominio PLAT/LH2 de proteínas relacionadas con la lipoxigenasa vegetal. Su descripción en pimienta negra, es esencial para aclarar los mecanismos moleculares de respuesta de la planta al hongo, y entender su papel en la activación de la respuesta de defensa, ya que el gen de Lox se activa en la planta, para señalar su defensa, en un posible ataque contra un patógeno y pueden ser precursores de reguladores metabólicos.A pimenta-do-reino é uma especiaria que tem importância econômica no mundo por sua ampla aplicação na indústria e pelas propriedades medicinais. O estado do Pará é um dos maiores produtores dessa especiaria, mas a produção dessa espécie é comprometida pela fusariose, que causa a podridão radicular na planta. O estudo da biologia molecular é importante para apoiar programas de melhoramento genético em pimenta-do-reino. Neste trabalho desenhamos os primers Lox3R e Lox3F para amplificar um fragmento maior do gene lipoxigenase (Lox) em pimenta-do-reino. O fragmento amplificado usando iniciadores desenhados foi sequenciado. A sequência isolada tem entorno de 770 nucleotídeos que codificam 258 aminoácidos. Esta sequência foi caracterizada por comparação em bancos de dados biológicos e utilizando programas computacionais. A análise com BlastX mostrou que a sequência isolada apresenta alta similaridade com proteínas lipoxigenases de Persea americana, Parasponia andersonii e Vitis vinífera. Verificamos que a Lox da pimenta-do-reino possui o domínio PLAT/LH2 de proteínas relacionadas à lipoxigenase vegetal. Sua descrição em pimenta-do-reino é essencial para esclarecer os mecanismos moleculares de resposta da planta ao fungo e entender seu papel na ativação da resposta de defesa, uma vez que o gene Lox é ativado na planta para sinalizar sua defesa em um possível ataque contra patógeno e podem ser precursores de reguladores metabólicos.Research, Society and Development2022-07-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3225410.33448/rsd-v11i9.32254Research, Society and Development; Vol. 11 No. 9; e57411932254Research, Society and Development; Vol. 11 Núm. 9; e57411932254Research, Society and Development; v. 11 n. 9; e574119322542525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/32254/27442Copyright (c) 2022 Edith Cibelle de Oliveira Moreira; Rafael Neves Pereira; Sheila Maysa da Cunha Gordo; Ilmarina Campus de Menezes; Cleonilde da Conceição Silva Queiroz; Divinno Bruno da Cunhahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMoreira, Edith Cibelle de OliveiraPereira, Rafael NevesGordo, Sheila Maysa da CunhaMenezes, Ilmarina Campus deQueiroz, Cleonilde da Conceição SilvaCunha, Divinno Bruno da2022-07-21T12:36:16Zoai:ojs.pkp.sfu.ca:article/32254Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:48:18.756622Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Isolation and partial characterization of the lipoxygenase gene in black pepper (Piper nigrum L) Aislamiento y caracterización parcial del gen de la lipoxigenasa en pimienta negra (Piper nigrum L) Isolamento e caracterização parcial do gene lipoxigenase em pimenta-do-reino (Piper nigrum L) |
title |
Isolation and partial characterization of the lipoxygenase gene in black pepper (Piper nigrum L) |
spellingShingle |
Isolation and partial characterization of the lipoxygenase gene in black pepper (Piper nigrum L) Moreira, Edith Cibelle de Oliveira Gene lipoxygenase Piper nigrum L. Defense Response. Gen de la lipoxigenasa Piper nigrum L. Defensa Respuesta. Gene lipoxigenase Piper nigrum L. Defesa Resposta. |
title_short |
Isolation and partial characterization of the lipoxygenase gene in black pepper (Piper nigrum L) |
title_full |
Isolation and partial characterization of the lipoxygenase gene in black pepper (Piper nigrum L) |
title_fullStr |
Isolation and partial characterization of the lipoxygenase gene in black pepper (Piper nigrum L) |
title_full_unstemmed |
Isolation and partial characterization of the lipoxygenase gene in black pepper (Piper nigrum L) |
title_sort |
Isolation and partial characterization of the lipoxygenase gene in black pepper (Piper nigrum L) |
author |
Moreira, Edith Cibelle de Oliveira |
author_facet |
Moreira, Edith Cibelle de Oliveira Pereira, Rafael Neves Gordo, Sheila Maysa da Cunha Menezes, Ilmarina Campus de Queiroz, Cleonilde da Conceição Silva Cunha, Divinno Bruno da |
author_role |
author |
author2 |
Pereira, Rafael Neves Gordo, Sheila Maysa da Cunha Menezes, Ilmarina Campus de Queiroz, Cleonilde da Conceição Silva Cunha, Divinno Bruno da |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Moreira, Edith Cibelle de Oliveira Pereira, Rafael Neves Gordo, Sheila Maysa da Cunha Menezes, Ilmarina Campus de Queiroz, Cleonilde da Conceição Silva Cunha, Divinno Bruno da |
dc.subject.por.fl_str_mv |
Gene lipoxygenase Piper nigrum L. Defense Response. Gen de la lipoxigenasa Piper nigrum L. Defensa Respuesta. Gene lipoxigenase Piper nigrum L. Defesa Resposta. |
topic |
Gene lipoxygenase Piper nigrum L. Defense Response. Gen de la lipoxigenasa Piper nigrum L. Defensa Respuesta. Gene lipoxigenase Piper nigrum L. Defesa Resposta. |
description |
Black pepper is a spice that have an economic importance in the world by its wide application in industry and by medicinal properties. State of Pará is one of the largest producers of these spices, but the production of this species is committed by fusariosis, that causes root rot in plant. Study of molecular biology is important by support breeding improvement of black pepper. In this work we draw the primers Lox3R and Lox3F to amplify a larger fragment of the gene lipoxygenase (Lox) in black pepper. The amplified fragment using primers drawn was sequenced. The sequence isolated has 770 nucleotides that encode 258 amino acids. This sequence was characterized by comparison in biological databases and using computational programs. The analysis with BlastX showed that sequence isolated has high similarity with lipoxygenase proteins of Persea americana, Parasponia andersonii e Vitis vinífera. We verified that Lox of black pepper has the PLAT/ LH2 domain of plant lipoxygenase related proteins. Its description in black pepper is essential to clarify the molecular mechanisms of response of the plant to the Fungus and understand its paper in activation of the defense response, once the gene Lox is activated in plant to signal its defense in a possible attack against pathogen and may be precursors of metabolic regulators. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-19 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/32254 10.33448/rsd-v11i9.32254 |
url |
https://rsdjournal.org/index.php/rsd/article/view/32254 |
identifier_str_mv |
10.33448/rsd-v11i9.32254 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/32254/27442 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 9; e57411932254 Research, Society and Development; Vol. 11 Núm. 9; e57411932254 Research, Society and Development; v. 11 n. 9; e57411932254 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052836865900544 |