Effect of the essential oil of palmaroSA (Cymbopogon martinii) on fungos phitopatogenic in soybean seeds

Detalhes bibliográficos
Autor(a) principal: Xavier, Alda Leaby dos Santos
Data de Publicação: 2020
Outros Autores: França, Kevison Romulo da Silva, Cardoso, Tiago Augusto Lima
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/8660
Resumo: This research evaluates the fungitoxic in vitro and in vivo effects of palmarosa essential oil (Cymbopogon martinii) on phytopathogenic fungi that infect soybean culture. In the in vitro test, the fungi were grown in PDA culture medium supplemented with pure essential oil in different concentrations (0.0125; 0.025; 0.05; 0.1; and 0.2%), the negative control (0%), and positive control (commercial fungicide Thiram 1 mL-1). The plates were inoculated with the fungi Phomopsis sp., Fusarium solani, Sclerotium rolfsii, and Macrophomina phaseolina. All plates were incubated for seven days at 27±2°C. In the in vivo test, soybean seeds were immersed in solutions with different palmarosa essential oil concentrations (0.05; 0.1; 0.2; 1.0; and 5.0%). After treatment, the seeds were artificially infected with the fungi under study, through contact with fungal colonies for 24 hours. The seeds were submitted to the sanity test and evaluated individually, using a stereoscopic microscope. Under in vitro conditions, the essential oil in concentrations from 0.05 to 0.1% precluded all studied fungi' growth. In the in vivo test, the treatments with concentrations of 1% and 5% reduced the percentage of seeds infected by S. rolfsii, M. phaseolina e Phomopsis sp. but were unsuccessful against F. solani. The essential oil of palmarosa is a promising alternative to the use of conventional fungicides in soybean seeds.
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spelling Effect of the essential oil of palmaroSA (Cymbopogon martinii) on fungos phitopatogenic in soybean seedsEfecto del aceite esencial de palmarosa (Cymbopogon martinii) sobre fungos fitopatogenicos en semillas de sojaEfeito do óleo essencial de palmarosa (Cymbopogon martinii) sobre fungos fitopatogênicos em sementes de sojaBiotecnologiaInibição fúngicaControle de pragas.BiotechnologyFungal inhibitionPest Control.BiotecnologíaInhibición de hongosControl de plagas.This research evaluates the fungitoxic in vitro and in vivo effects of palmarosa essential oil (Cymbopogon martinii) on phytopathogenic fungi that infect soybean culture. In the in vitro test, the fungi were grown in PDA culture medium supplemented with pure essential oil in different concentrations (0.0125; 0.025; 0.05; 0.1; and 0.2%), the negative control (0%), and positive control (commercial fungicide Thiram 1 mL-1). The plates were inoculated with the fungi Phomopsis sp., Fusarium solani, Sclerotium rolfsii, and Macrophomina phaseolina. All plates were incubated for seven days at 27±2°C. In the in vivo test, soybean seeds were immersed in solutions with different palmarosa essential oil concentrations (0.05; 0.1; 0.2; 1.0; and 5.0%). After treatment, the seeds were artificially infected with the fungi under study, through contact with fungal colonies for 24 hours. The seeds were submitted to the sanity test and evaluated individually, using a stereoscopic microscope. Under in vitro conditions, the essential oil in concentrations from 0.05 to 0.1% precluded all studied fungi' growth. In the in vivo test, the treatments with concentrations of 1% and 5% reduced the percentage of seeds infected by S. rolfsii, M. phaseolina e Phomopsis sp. but were unsuccessful against F. solani. The essential oil of palmarosa is a promising alternative to the use of conventional fungicides in soybean seeds.Esta investigación evalúa los efectos fungitóxicos in vitro e in vivo del aceite esencial de palmarosa (Cymbopogon martinii) sobre hongos fitopatógenos que infectan los cultivos de soja. En la prueba in vitro, los hongos se cultivaron en medio de cultivo BDA suplementado con aceite esencial puro en diferentes concentraciones (0.0125; 0.025; 0.05; 0.1; y 0.2%), control negativo (0%) y control positivo (fungicida comercial Thiram 1 mL-1). Las placas se inocularon con los hongos Phomopsis sp., Fusarium solani, Sclerotium rolfsii y Macrophomina phaseolina, durante siete días a 27 ± 2 ° C. En la prueba in vivo, las semillas de soja se sumergieron en soluciones con diferentes concentraciones de aceite esencial de palmarosa (0.05; 0.1; 0.2; 1, 0; y 5,0%). Después del tratamiento, las semillas se infectaron artificialmente con los hongos en estudio, a través del contacto con colonias de hongos durante 24 horas. Las semillas se sometieron a la prueba de cordura y se evaluaron individualmente, bajo un microscopio estereoscópico. En condiciones in vitro, el aceite esencial en concentraciones de 0.05 a 0.1% impidió el crecimiento de todos los hongos estudiados. En la prueba in vivo, los tratamientos con concentraciones de 1% y 5% redujeron el porcentaje de semillas infectadas por S. rolfsii, M. phaseolina y Phomopsis sp. pero no triunfaron contra F. solani. El aceite esencial de palmarosa es una alternativa prometedora al uso de fungicidas convencionales en semillas de soja.Esta pesquisa avalia os efeitos fungitóxicos in vitro e in vivo do óleo essencial de palmarosa (Cymbopogon martinii) sobre fungos fitopatogênicos que infectam a cultura da soja. No teste in vitro, os fungos foram cultivados em meio de cultura BDA suplementado com óleo essencial puro em diferentes concentrações (0,0125; 0,025; 0,05; 0,1; e 0,2%), controle negativo (0%) e controle positivo (fungicida comercial Thiram 1 mL-1). As placas foram inoculadas com os fungos Phomopsis sp., Fusarium solani, Sclerotium rolfsii e Macrophomina phaseolina, durante sete dias a 27 ± 2 ° C. No teste in vivo, sementes de soja foram imersas em soluções com diferentes concentrações de óleo essencial de palmarosa (0,05; 0,1; 0,2; 1,0; e 5,0%). Após o tratamento, as sementes foram infectadas artificialmente com os fungos em estudo, por meio do contato com colônias fúngicas por 24 horas. As sementes foram submetidas ao teste de sanidade e avaliadas individualmente, em microscópio estereoscópico. Em condições in vitro, o óleo essencial nas concentrações de 0,05 a 0,1% impediu o crescimento de todos os fungos estudados. No teste in vivo, os tratamentos com concentrações de 1% e 5% reduziram a porcentagem de sementes infectadas por S. rolfsii, M. phaseolina e Phomopsis sp. mas não tiveram sucesso contra F. solani. O óleo essencial de palmarosa é uma alternativa promissora ao uso de fungicidas convencionais em sementes de soja.Research, Society and Development2020-10-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/866010.33448/rsd-v9i10.8660Research, Society and Development; Vol. 9 No. 10; e4529108660Research, Society and Development; Vol. 9 Núm. 10; e4529108660Research, Society and Development; v. 9 n. 10; e45291086602525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/8660/7795Copyright (c) 2020 Alda Leaby dos Santos Xavier; Kevison Romulo da Silva França; Tiago Augusto Lima Cardosohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess Xavier, Alda Leaby dos SantosFrança, Kevison Romulo da SilvaCardoso, Tiago Augusto Lima2020-10-31T12:03:23Zoai:ojs.pkp.sfu.ca:article/8660Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:31:05.225631Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Effect of the essential oil of palmaroSA (Cymbopogon martinii) on fungos phitopatogenic in soybean seeds
Efecto del aceite esencial de palmarosa (Cymbopogon martinii) sobre fungos fitopatogenicos en semillas de soja
Efeito do óleo essencial de palmarosa (Cymbopogon martinii) sobre fungos fitopatogênicos em sementes de soja
title Effect of the essential oil of palmaroSA (Cymbopogon martinii) on fungos phitopatogenic in soybean seeds
spellingShingle Effect of the essential oil of palmaroSA (Cymbopogon martinii) on fungos phitopatogenic in soybean seeds
Xavier, Alda Leaby dos Santos
Biotecnologia
Inibição fúngica
Controle de pragas.
Biotechnology
Fungal inhibition
Pest Control.
Biotecnología
Inhibición de hongos
Control de plagas.
title_short Effect of the essential oil of palmaroSA (Cymbopogon martinii) on fungos phitopatogenic in soybean seeds
title_full Effect of the essential oil of palmaroSA (Cymbopogon martinii) on fungos phitopatogenic in soybean seeds
title_fullStr Effect of the essential oil of palmaroSA (Cymbopogon martinii) on fungos phitopatogenic in soybean seeds
title_full_unstemmed Effect of the essential oil of palmaroSA (Cymbopogon martinii) on fungos phitopatogenic in soybean seeds
title_sort Effect of the essential oil of palmaroSA (Cymbopogon martinii) on fungos phitopatogenic in soybean seeds
author Xavier, Alda Leaby dos Santos
author_facet Xavier, Alda Leaby dos Santos
França, Kevison Romulo da Silva
Cardoso, Tiago Augusto Lima
author_role author
author2 França, Kevison Romulo da Silva
Cardoso, Tiago Augusto Lima
author2_role author
author
dc.contributor.author.fl_str_mv Xavier, Alda Leaby dos Santos
França, Kevison Romulo da Silva
Cardoso, Tiago Augusto Lima
dc.subject.por.fl_str_mv Biotecnologia
Inibição fúngica
Controle de pragas.
Biotechnology
Fungal inhibition
Pest Control.
Biotecnología
Inhibición de hongos
Control de plagas.
topic Biotecnologia
Inibição fúngica
Controle de pragas.
Biotechnology
Fungal inhibition
Pest Control.
Biotecnología
Inhibición de hongos
Control de plagas.
description This research evaluates the fungitoxic in vitro and in vivo effects of palmarosa essential oil (Cymbopogon martinii) on phytopathogenic fungi that infect soybean culture. In the in vitro test, the fungi were grown in PDA culture medium supplemented with pure essential oil in different concentrations (0.0125; 0.025; 0.05; 0.1; and 0.2%), the negative control (0%), and positive control (commercial fungicide Thiram 1 mL-1). The plates were inoculated with the fungi Phomopsis sp., Fusarium solani, Sclerotium rolfsii, and Macrophomina phaseolina. All plates were incubated for seven days at 27±2°C. In the in vivo test, soybean seeds were immersed in solutions with different palmarosa essential oil concentrations (0.05; 0.1; 0.2; 1.0; and 5.0%). After treatment, the seeds were artificially infected with the fungi under study, through contact with fungal colonies for 24 hours. The seeds were submitted to the sanity test and evaluated individually, using a stereoscopic microscope. Under in vitro conditions, the essential oil in concentrations from 0.05 to 0.1% precluded all studied fungi' growth. In the in vivo test, the treatments with concentrations of 1% and 5% reduced the percentage of seeds infected by S. rolfsii, M. phaseolina e Phomopsis sp. but were unsuccessful against F. solani. The essential oil of palmarosa is a promising alternative to the use of conventional fungicides in soybean seeds.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/8660
10.33448/rsd-v9i10.8660
url https://rsdjournal.org/index.php/rsd/article/view/8660
identifier_str_mv 10.33448/rsd-v9i10.8660
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/8660/7795
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 9 No. 10; e4529108660
Research, Society and Development; Vol. 9 Núm. 10; e4529108660
Research, Society and Development; v. 9 n. 10; e4529108660
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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