Effect of the essential oil of palmaroSA (Cymbopogon martinii) on fungos phitopatogenic in soybean seeds
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/8660 |
Resumo: | This research evaluates the fungitoxic in vitro and in vivo effects of palmarosa essential oil (Cymbopogon martinii) on phytopathogenic fungi that infect soybean culture. In the in vitro test, the fungi were grown in PDA culture medium supplemented with pure essential oil in different concentrations (0.0125; 0.025; 0.05; 0.1; and 0.2%), the negative control (0%), and positive control (commercial fungicide Thiram 1 mL-1). The plates were inoculated with the fungi Phomopsis sp., Fusarium solani, Sclerotium rolfsii, and Macrophomina phaseolina. All plates were incubated for seven days at 27±2°C. In the in vivo test, soybean seeds were immersed in solutions with different palmarosa essential oil concentrations (0.05; 0.1; 0.2; 1.0; and 5.0%). After treatment, the seeds were artificially infected with the fungi under study, through contact with fungal colonies for 24 hours. The seeds were submitted to the sanity test and evaluated individually, using a stereoscopic microscope. Under in vitro conditions, the essential oil in concentrations from 0.05 to 0.1% precluded all studied fungi' growth. In the in vivo test, the treatments with concentrations of 1% and 5% reduced the percentage of seeds infected by S. rolfsii, M. phaseolina e Phomopsis sp. but were unsuccessful against F. solani. The essential oil of palmarosa is a promising alternative to the use of conventional fungicides in soybean seeds. |
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Effect of the essential oil of palmaroSA (Cymbopogon martinii) on fungos phitopatogenic in soybean seedsEfecto del aceite esencial de palmarosa (Cymbopogon martinii) sobre fungos fitopatogenicos en semillas de sojaEfeito do óleo essencial de palmarosa (Cymbopogon martinii) sobre fungos fitopatogênicos em sementes de sojaBiotecnologiaInibição fúngicaControle de pragas.BiotechnologyFungal inhibitionPest Control.BiotecnologíaInhibición de hongosControl de plagas.This research evaluates the fungitoxic in vitro and in vivo effects of palmarosa essential oil (Cymbopogon martinii) on phytopathogenic fungi that infect soybean culture. In the in vitro test, the fungi were grown in PDA culture medium supplemented with pure essential oil in different concentrations (0.0125; 0.025; 0.05; 0.1; and 0.2%), the negative control (0%), and positive control (commercial fungicide Thiram 1 mL-1). The plates were inoculated with the fungi Phomopsis sp., Fusarium solani, Sclerotium rolfsii, and Macrophomina phaseolina. All plates were incubated for seven days at 27±2°C. In the in vivo test, soybean seeds were immersed in solutions with different palmarosa essential oil concentrations (0.05; 0.1; 0.2; 1.0; and 5.0%). After treatment, the seeds were artificially infected with the fungi under study, through contact with fungal colonies for 24 hours. The seeds were submitted to the sanity test and evaluated individually, using a stereoscopic microscope. Under in vitro conditions, the essential oil in concentrations from 0.05 to 0.1% precluded all studied fungi' growth. In the in vivo test, the treatments with concentrations of 1% and 5% reduced the percentage of seeds infected by S. rolfsii, M. phaseolina e Phomopsis sp. but were unsuccessful against F. solani. The essential oil of palmarosa is a promising alternative to the use of conventional fungicides in soybean seeds.Esta investigación evalúa los efectos fungitóxicos in vitro e in vivo del aceite esencial de palmarosa (Cymbopogon martinii) sobre hongos fitopatógenos que infectan los cultivos de soja. En la prueba in vitro, los hongos se cultivaron en medio de cultivo BDA suplementado con aceite esencial puro en diferentes concentraciones (0.0125; 0.025; 0.05; 0.1; y 0.2%), control negativo (0%) y control positivo (fungicida comercial Thiram 1 mL-1). Las placas se inocularon con los hongos Phomopsis sp., Fusarium solani, Sclerotium rolfsii y Macrophomina phaseolina, durante siete días a 27 ± 2 ° C. En la prueba in vivo, las semillas de soja se sumergieron en soluciones con diferentes concentraciones de aceite esencial de palmarosa (0.05; 0.1; 0.2; 1, 0; y 5,0%). Después del tratamiento, las semillas se infectaron artificialmente con los hongos en estudio, a través del contacto con colonias de hongos durante 24 horas. Las semillas se sometieron a la prueba de cordura y se evaluaron individualmente, bajo un microscopio estereoscópico. En condiciones in vitro, el aceite esencial en concentraciones de 0.05 a 0.1% impidió el crecimiento de todos los hongos estudiados. En la prueba in vivo, los tratamientos con concentraciones de 1% y 5% redujeron el porcentaje de semillas infectadas por S. rolfsii, M. phaseolina y Phomopsis sp. pero no triunfaron contra F. solani. El aceite esencial de palmarosa es una alternativa prometedora al uso de fungicidas convencionales en semillas de soja.Esta pesquisa avalia os efeitos fungitóxicos in vitro e in vivo do óleo essencial de palmarosa (Cymbopogon martinii) sobre fungos fitopatogênicos que infectam a cultura da soja. No teste in vitro, os fungos foram cultivados em meio de cultura BDA suplementado com óleo essencial puro em diferentes concentrações (0,0125; 0,025; 0,05; 0,1; e 0,2%), controle negativo (0%) e controle positivo (fungicida comercial Thiram 1 mL-1). As placas foram inoculadas com os fungos Phomopsis sp., Fusarium solani, Sclerotium rolfsii e Macrophomina phaseolina, durante sete dias a 27 ± 2 ° C. No teste in vivo, sementes de soja foram imersas em soluções com diferentes concentrações de óleo essencial de palmarosa (0,05; 0,1; 0,2; 1,0; e 5,0%). Após o tratamento, as sementes foram infectadas artificialmente com os fungos em estudo, por meio do contato com colônias fúngicas por 24 horas. As sementes foram submetidas ao teste de sanidade e avaliadas individualmente, em microscópio estereoscópico. Em condições in vitro, o óleo essencial nas concentrações de 0,05 a 0,1% impediu o crescimento de todos os fungos estudados. No teste in vivo, os tratamentos com concentrações de 1% e 5% reduziram a porcentagem de sementes infectadas por S. rolfsii, M. phaseolina e Phomopsis sp. mas não tiveram sucesso contra F. solani. O óleo essencial de palmarosa é uma alternativa promissora ao uso de fungicidas convencionais em sementes de soja.Research, Society and Development2020-10-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/866010.33448/rsd-v9i10.8660Research, Society and Development; Vol. 9 No. 10; e4529108660Research, Society and Development; Vol. 9 Núm. 10; e4529108660Research, Society and Development; v. 9 n. 10; e45291086602525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/8660/7795Copyright (c) 2020 Alda Leaby dos Santos Xavier; Kevison Romulo da Silva França; Tiago Augusto Lima Cardosohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess Xavier, Alda Leaby dos SantosFrança, Kevison Romulo da SilvaCardoso, Tiago Augusto Lima2020-10-31T12:03:23Zoai:ojs.pkp.sfu.ca:article/8660Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:31:05.225631Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Effect of the essential oil of palmaroSA (Cymbopogon martinii) on fungos phitopatogenic in soybean seeds Efecto del aceite esencial de palmarosa (Cymbopogon martinii) sobre fungos fitopatogenicos en semillas de soja Efeito do óleo essencial de palmarosa (Cymbopogon martinii) sobre fungos fitopatogênicos em sementes de soja |
title |
Effect of the essential oil of palmaroSA (Cymbopogon martinii) on fungos phitopatogenic in soybean seeds |
spellingShingle |
Effect of the essential oil of palmaroSA (Cymbopogon martinii) on fungos phitopatogenic in soybean seeds Xavier, Alda Leaby dos Santos Biotecnologia Inibição fúngica Controle de pragas. Biotechnology Fungal inhibition Pest Control. Biotecnología Inhibición de hongos Control de plagas. |
title_short |
Effect of the essential oil of palmaroSA (Cymbopogon martinii) on fungos phitopatogenic in soybean seeds |
title_full |
Effect of the essential oil of palmaroSA (Cymbopogon martinii) on fungos phitopatogenic in soybean seeds |
title_fullStr |
Effect of the essential oil of palmaroSA (Cymbopogon martinii) on fungos phitopatogenic in soybean seeds |
title_full_unstemmed |
Effect of the essential oil of palmaroSA (Cymbopogon martinii) on fungos phitopatogenic in soybean seeds |
title_sort |
Effect of the essential oil of palmaroSA (Cymbopogon martinii) on fungos phitopatogenic in soybean seeds |
author |
Xavier, Alda Leaby dos Santos |
author_facet |
Xavier, Alda Leaby dos Santos França, Kevison Romulo da Silva Cardoso, Tiago Augusto Lima |
author_role |
author |
author2 |
França, Kevison Romulo da Silva Cardoso, Tiago Augusto Lima |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Xavier, Alda Leaby dos Santos França, Kevison Romulo da Silva Cardoso, Tiago Augusto Lima |
dc.subject.por.fl_str_mv |
Biotecnologia Inibição fúngica Controle de pragas. Biotechnology Fungal inhibition Pest Control. Biotecnología Inhibición de hongos Control de plagas. |
topic |
Biotecnologia Inibição fúngica Controle de pragas. Biotechnology Fungal inhibition Pest Control. Biotecnología Inhibición de hongos Control de plagas. |
description |
This research evaluates the fungitoxic in vitro and in vivo effects of palmarosa essential oil (Cymbopogon martinii) on phytopathogenic fungi that infect soybean culture. In the in vitro test, the fungi were grown in PDA culture medium supplemented with pure essential oil in different concentrations (0.0125; 0.025; 0.05; 0.1; and 0.2%), the negative control (0%), and positive control (commercial fungicide Thiram 1 mL-1). The plates were inoculated with the fungi Phomopsis sp., Fusarium solani, Sclerotium rolfsii, and Macrophomina phaseolina. All plates were incubated for seven days at 27±2°C. In the in vivo test, soybean seeds were immersed in solutions with different palmarosa essential oil concentrations (0.05; 0.1; 0.2; 1.0; and 5.0%). After treatment, the seeds were artificially infected with the fungi under study, through contact with fungal colonies for 24 hours. The seeds were submitted to the sanity test and evaluated individually, using a stereoscopic microscope. Under in vitro conditions, the essential oil in concentrations from 0.05 to 0.1% precluded all studied fungi' growth. In the in vivo test, the treatments with concentrations of 1% and 5% reduced the percentage of seeds infected by S. rolfsii, M. phaseolina e Phomopsis sp. but were unsuccessful against F. solani. The essential oil of palmarosa is a promising alternative to the use of conventional fungicides in soybean seeds. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/8660 10.33448/rsd-v9i10.8660 |
url |
https://rsdjournal.org/index.php/rsd/article/view/8660 |
identifier_str_mv |
10.33448/rsd-v9i10.8660 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/8660/7795 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 9 No. 10; e4529108660 Research, Society and Development; Vol. 9 Núm. 10; e4529108660 Research, Society and Development; v. 9 n. 10; e4529108660 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052740759715840 |