Photoprotective activity of geopropolis produced by Melipona subnitida (Apidae, Meliponinae) in the semiarid of the Brazilian Northeast
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/12305 |
Resumo: | Although geopropolis has been the subject of many chemical and pharmacological studies, there are few studies investigating the photoprotective activity of formulations containing propolis. Thus, we investigated in vivo the photoprotective efficacy of the cream containing geopropolis extract by macroscopic and histological evaluation of the skin of Wistar rats subjected to ultraviolet radiation (UVB). We also evaluated the chemical composition of hydroethanolic extract using the HPLC-DADESI-MS/MS technique, as well as antioxidant activity by the photocolorimetric method of free radical DPPH (2.2-diphenyl-1-picrylhydrazine) and cytotoxic activity by the in vitro MTT quantitative method [brometo de 3- (4.5dimetiltiazol-2-il)-2.5-difeniltetrazolio]. The extract had a varied chemical composition, 29 different phenolic compounds being detected, distributed between phenols and flavonoids, the latter being represented by chalcones, flavones and flavonols. The highest percentages of DPPH inhibition e o baixo valor de IC50 indicaram que o extrato apresentou alta atividade antioxidante. The hydroethanolic extract did not exert cytotoxic effects since high percentages of viability of L929 fibroblasts were observed after incubation for 72 hours at different concentrations of the extract. On skin submitted to cream application containing of geopropolis extract and the irradiation with UVB did not occur macroscopic and histological lesions. Thus, we concluded that the cream containing of geopropolis extract produced by Melipona subnitida was able to protect the skin from lesions induced by UVB irradiation, thus demonstrating photoprotective effect. |
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Photoprotective activity of geopropolis produced by Melipona subnitida (Apidae, Meliponinae) in the semiarid of the Brazilian NortheastActividad fotoprotectora de geopropóleos producidos por Melipona subnitida (Apidae, Meliponinae) en el semiárido del Nordeste brasileñoAtividade fotoprotetora de geoprópolis produzida por Melipona subnitida (Apidae, Meliponinae) no semiárido do Nordeste brasileiroMeliponinaeGeoprópolisEfeito fotoprotetorTeor de fenolAção antioxidanteFator de proteção solar.MeliponinaeGeopropolisEfecto fotoprotectorContenido de fenolAcción antioxidanteFactor de protección solar.MeliponinaeGeopropolisPhotoprotective effectPhenol contentsAntioxidant actionSun protection factor.Although geopropolis has been the subject of many chemical and pharmacological studies, there are few studies investigating the photoprotective activity of formulations containing propolis. Thus, we investigated in vivo the photoprotective efficacy of the cream containing geopropolis extract by macroscopic and histological evaluation of the skin of Wistar rats subjected to ultraviolet radiation (UVB). We also evaluated the chemical composition of hydroethanolic extract using the HPLC-DADESI-MS/MS technique, as well as antioxidant activity by the photocolorimetric method of free radical DPPH (2.2-diphenyl-1-picrylhydrazine) and cytotoxic activity by the in vitro MTT quantitative method [brometo de 3- (4.5dimetiltiazol-2-il)-2.5-difeniltetrazolio]. The extract had a varied chemical composition, 29 different phenolic compounds being detected, distributed between phenols and flavonoids, the latter being represented by chalcones, flavones and flavonols. The highest percentages of DPPH inhibition e o baixo valor de IC50 indicaram que o extrato apresentou alta atividade antioxidante. The hydroethanolic extract did not exert cytotoxic effects since high percentages of viability of L929 fibroblasts were observed after incubation for 72 hours at different concentrations of the extract. On skin submitted to cream application containing of geopropolis extract and the irradiation with UVB did not occur macroscopic and histological lesions. Thus, we concluded that the cream containing of geopropolis extract produced by Melipona subnitida was able to protect the skin from lesions induced by UVB irradiation, thus demonstrating photoprotective effect.Aunque el geopropóleo ha sido objeto de muchos estudios químicos y farmacológicos, existen pocos estudios que investiguen la actividad fotoprotectora de formulaciones que contienen propóleos. Así, investigamos in vivo la eficacia fotoprotectora de la crema que contiene extracto de geopropóleo mediante evaluación macroscópica e histológica de la piel de ratas Wistar sometidas a radiación ultravioleta (UVB). También evaluamos la composición química del extracto hidroetanólico mediante la técnica HPLC-DADESI-MS / MS, así como la actividad antioxidante por el método fotocolorimétrico del radical libre DPPH (2.2-difenil-1-picrilhidrazina) y la actividad citotóxica por el MTT in vitro método cuantitativo [brometo de 3- (4,5-dimetiltiazol-2-il) -2,5-difeniltetrazolio]. El extracto tuvo una composición química variada, detectándose 29 compuestos fenólicos diferentes, distribuidos entre fenoles y flavonoides, estos últimos representados por chalconas, flavonas y flavonoles. Los porcentajes más altos de inhibición de DPPH e o baixo valor de IC50 indicaram que o extrato apresentou alta atividade antioxidante. El extracto hidroetanólico no ejerció efectos citotóxicos ya que se observaron altos porcentajes de viabilidad de los fibroblastos L929 después de la incubación durante 72 horas a diferentes concentraciones del extracto. Sobre la piel sometida a la aplicación de una crema que contenía extracto de geopropóleo y la irradiación con UVB no se produjeron lesiones macroscópicas e histológicas. Así, concluimos que la crema que contiene extracto de geopropóleo producida por Melipona subnitida fue capaz de proteger la piel de las lesiones inducidas por la irradiación UVB, demostrando así un efecto fotoprotector.Embora a geoprópolis tenha sido objeto de muitos estudos químicos e farmacológicos, poucos são os estudos que investigam a atividade fotoprotetora de formulações contendo própolis. Assim, investigamos in vivo a eficácia fotoprotetora do creme contendo extrato de geoprópolis por meio da avaliação macroscópica e histológica da pele de ratos Wistar submetidos à radiação ultravioleta (UVB). Também avaliamos a composição química do extrato hidroetanólico pela técnica de HPLC-DADESI-MS / MS, bem como a atividade antioxidante pelo método fotocolorimétrico do radical livre DPPH (2,2-difenil-1-picrilhidrazina) e a atividade citotóxica pelo MTT in vitro método quantitativo [brometo de 3- (4,5dimetiltiazol-2-il) -2,5-difeniltetrazólio]. O extrato apresentou composição química variada, sendo detectados 29 compostos fenólicos diferentes, distribuídos entre fenóis e flavonóides, sendo estes últimos representados por chalconas, flavonas e flavonóis. Os maiores percentuais de inibição do DPPH e o baixo valor de IC50 indicaram que o extrato apresenta alta atividade antioxidante. O extrato hidroetanólico não exerceu efeito citotóxico, pois altas porcentagens de viabilidade de fibroblastos L929 foram observadas após incubação por 72 horas em diferentes concentrações do extrato. Na pele submetida à aplicação de creme contendo extrato de geoprópolis e à irradiação com UVB não ocorreram lesões macroscópicas e histológicas. Assim, concluímos que o creme contendo extrato de geoprópolis produzido por Melipona subnitida foi capaz de proteger a pele das lesões induzidas pela irradiação UVB, demonstrando, assim, efeito fotoprotetor.Research, Society and Development2021-02-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1230510.33448/rsd-v10i2.12305Research, Society and Development; Vol. 10 No. 2; e11210212305Research, Society and Development; Vol. 10 Núm. 2; e11210212305Research, Society and Development; v. 10 n. 2; e112102123052525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/12305/11088Copyright (c) 2021 Jael Soares Batista; Antonio Salatino; Giuseppina Negri; Carmen Eusebia P. Jara; Kaliane Alessandra R. de Paiva; Wanderson Lucas Alves dos Santos; Tiago da Silva Teófilo; Natanael Silva Félix; Francisco Herbeson Aquino Silva; Victor Hugo V. Rodrigueshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBatista, Jael Soares Salatino, AntonioNegri, GiuseppinaJara, Carmen Eusebia P. Paiva, Kaliane Alessandra R. deSantos, Wanderson Lucas Alves dos Teófilo, Tiago da Silva Félix, Natanael Silva Silva, Francisco Herbeson Aquino Rodrigues, Victor Hugo V. 2021-03-02T09:32:39Zoai:ojs.pkp.sfu.ca:article/12305Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:33:55.296954Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Photoprotective activity of geopropolis produced by Melipona subnitida (Apidae, Meliponinae) in the semiarid of the Brazilian Northeast Actividad fotoprotectora de geopropóleos producidos por Melipona subnitida (Apidae, Meliponinae) en el semiárido del Nordeste brasileño Atividade fotoprotetora de geoprópolis produzida por Melipona subnitida (Apidae, Meliponinae) no semiárido do Nordeste brasileiro |
title |
Photoprotective activity of geopropolis produced by Melipona subnitida (Apidae, Meliponinae) in the semiarid of the Brazilian Northeast |
spellingShingle |
Photoprotective activity of geopropolis produced by Melipona subnitida (Apidae, Meliponinae) in the semiarid of the Brazilian Northeast Batista, Jael Soares Meliponinae Geoprópolis Efeito fotoprotetor Teor de fenol Ação antioxidante Fator de proteção solar. Meliponinae Geopropolis Efecto fotoprotector Contenido de fenol Acción antioxidante Factor de protección solar. Meliponinae Geopropolis Photoprotective effect Phenol contents Antioxidant action Sun protection factor. |
title_short |
Photoprotective activity of geopropolis produced by Melipona subnitida (Apidae, Meliponinae) in the semiarid of the Brazilian Northeast |
title_full |
Photoprotective activity of geopropolis produced by Melipona subnitida (Apidae, Meliponinae) in the semiarid of the Brazilian Northeast |
title_fullStr |
Photoprotective activity of geopropolis produced by Melipona subnitida (Apidae, Meliponinae) in the semiarid of the Brazilian Northeast |
title_full_unstemmed |
Photoprotective activity of geopropolis produced by Melipona subnitida (Apidae, Meliponinae) in the semiarid of the Brazilian Northeast |
title_sort |
Photoprotective activity of geopropolis produced by Melipona subnitida (Apidae, Meliponinae) in the semiarid of the Brazilian Northeast |
author |
Batista, Jael Soares |
author_facet |
Batista, Jael Soares Salatino, Antonio Negri, Giuseppina Jara, Carmen Eusebia P. Paiva, Kaliane Alessandra R. de Santos, Wanderson Lucas Alves dos Teófilo, Tiago da Silva Félix, Natanael Silva Silva, Francisco Herbeson Aquino Rodrigues, Victor Hugo V. |
author_role |
author |
author2 |
Salatino, Antonio Negri, Giuseppina Jara, Carmen Eusebia P. Paiva, Kaliane Alessandra R. de Santos, Wanderson Lucas Alves dos Teófilo, Tiago da Silva Félix, Natanael Silva Silva, Francisco Herbeson Aquino Rodrigues, Victor Hugo V. |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Batista, Jael Soares Salatino, Antonio Negri, Giuseppina Jara, Carmen Eusebia P. Paiva, Kaliane Alessandra R. de Santos, Wanderson Lucas Alves dos Teófilo, Tiago da Silva Félix, Natanael Silva Silva, Francisco Herbeson Aquino Rodrigues, Victor Hugo V. |
dc.subject.por.fl_str_mv |
Meliponinae Geoprópolis Efeito fotoprotetor Teor de fenol Ação antioxidante Fator de proteção solar. Meliponinae Geopropolis Efecto fotoprotector Contenido de fenol Acción antioxidante Factor de protección solar. Meliponinae Geopropolis Photoprotective effect Phenol contents Antioxidant action Sun protection factor. |
topic |
Meliponinae Geoprópolis Efeito fotoprotetor Teor de fenol Ação antioxidante Fator de proteção solar. Meliponinae Geopropolis Efecto fotoprotector Contenido de fenol Acción antioxidante Factor de protección solar. Meliponinae Geopropolis Photoprotective effect Phenol contents Antioxidant action Sun protection factor. |
description |
Although geopropolis has been the subject of many chemical and pharmacological studies, there are few studies investigating the photoprotective activity of formulations containing propolis. Thus, we investigated in vivo the photoprotective efficacy of the cream containing geopropolis extract by macroscopic and histological evaluation of the skin of Wistar rats subjected to ultraviolet radiation (UVB). We also evaluated the chemical composition of hydroethanolic extract using the HPLC-DADESI-MS/MS technique, as well as antioxidant activity by the photocolorimetric method of free radical DPPH (2.2-diphenyl-1-picrylhydrazine) and cytotoxic activity by the in vitro MTT quantitative method [brometo de 3- (4.5dimetiltiazol-2-il)-2.5-difeniltetrazolio]. The extract had a varied chemical composition, 29 different phenolic compounds being detected, distributed between phenols and flavonoids, the latter being represented by chalcones, flavones and flavonols. The highest percentages of DPPH inhibition e o baixo valor de IC50 indicaram que o extrato apresentou alta atividade antioxidante. The hydroethanolic extract did not exert cytotoxic effects since high percentages of viability of L929 fibroblasts were observed after incubation for 72 hours at different concentrations of the extract. On skin submitted to cream application containing of geopropolis extract and the irradiation with UVB did not occur macroscopic and histological lesions. Thus, we concluded that the cream containing of geopropolis extract produced by Melipona subnitida was able to protect the skin from lesions induced by UVB irradiation, thus demonstrating photoprotective effect. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-02-07 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/12305 10.33448/rsd-v10i2.12305 |
url |
https://rsdjournal.org/index.php/rsd/article/view/12305 |
identifier_str_mv |
10.33448/rsd-v10i2.12305 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/12305/11088 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 2; e11210212305 Research, Society and Development; Vol. 10 Núm. 2; e11210212305 Research, Society and Development; v. 10 n. 2; e11210212305 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052745491939328 |