Development and validation of analytical method by spectrophotometry UV-Vis for quantification of flavonoids in leaves of Senna occidentalis Link
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/18584 |
Resumo: | Senna occidentalis Link (Fabaceae), known as “fedegoso”, is used in folk medicine due to its anti-inflammatory, antioxidant, muscle relaxant and inhibiting lipid peroxidation imputed to flavonoids contained in its composition. The species is also a constituent of processed products are commercialized in various parts of the world including Brazil, although there are few reports in the literature about the development of an analytical method for quantification of flavonoids in it. The main purpose of this research was the evaluation of an analytical methodology to determinate the content of total flavonoids in leaves of S. occidentalis, for quality control. The parameters evaluated were: drug: solvent ratio, concentration of aluminum chloride and reaction time. The quantification of total flavonoids was obtained after reaction with aluminum chloride by UV/Vis spectrophotometry. The results revealed a maximum absorption peak (λ = 392 nm) was the amount of 0.5 g of a plant raw in 100 mL of solvent and there were no significant influences between the concentrations of aluminum chloride or hydroalcoholic solutions analyzed. The evaluation of reaction time allowed to characterize the kinetics as slow, stabilizing from 60 min, choosing this as reading time. The method was specific, linear, precise, accurate and robust, according to the specifications set in RDC n. 166/2017. Finally, the results of the study showed that the measured methodology is simple and accurate and can be applied for quality assessment of the species S. occidentalis. |
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Development and validation of analytical method by spectrophotometry UV-Vis for quantification of flavonoids in leaves of Senna occidentalis LinkDesarrollo y validación de método analítico por espectrofotometría UV-Vis para cuantificación de flavonoides en hojas de Senna occidentalis LinkDesenvolvimento e validação de método analítico por espectrofotometria UV-Vis para quantificação de flavonóides em folhas de Senna occidentalis LinkSenna occidentalisFlavonóides totaisEspectrofotometria ultravioleta-visívelValidação.Senna occidentalisFlavonoides totalesEspectrofotometría ultravioleta-visibleValidación.Senna occidentalisTotal flavonoidsUltraviolet-Visible spectrophotometryValidation.Senna occidentalis Link (Fabaceae), known as “fedegoso”, is used in folk medicine due to its anti-inflammatory, antioxidant, muscle relaxant and inhibiting lipid peroxidation imputed to flavonoids contained in its composition. The species is also a constituent of processed products are commercialized in various parts of the world including Brazil, although there are few reports in the literature about the development of an analytical method for quantification of flavonoids in it. The main purpose of this research was the evaluation of an analytical methodology to determinate the content of total flavonoids in leaves of S. occidentalis, for quality control. The parameters evaluated were: drug: solvent ratio, concentration of aluminum chloride and reaction time. The quantification of total flavonoids was obtained after reaction with aluminum chloride by UV/Vis spectrophotometry. The results revealed a maximum absorption peak (λ = 392 nm) was the amount of 0.5 g of a plant raw in 100 mL of solvent and there were no significant influences between the concentrations of aluminum chloride or hydroalcoholic solutions analyzed. The evaluation of reaction time allowed to characterize the kinetics as slow, stabilizing from 60 min, choosing this as reading time. The method was specific, linear, precise, accurate and robust, according to the specifications set in RDC n. 166/2017. Finally, the results of the study showed that the measured methodology is simple and accurate and can be applied for quality assessment of the species S. occidentalis.Senna occidentalis Link (Fabaceae), conocido como “fedegoso”, se utiliza en la medicina popular por su acción antiinflamatoria, antioxidante, relajante muscular e inhibidora de la peroxidación lipídica imputada a los flavonoides contenidos en su composición. La especie también es un constituyente de productos procesados que se comercializan en diversas partes del mundo incluido Brasil, aunque existen pocos informes en la literatura sobre el desarrollo de un método analítico para la cuantificación de flavonoides en ella. El objetivo principal de esta investigación fue la evaluación de una metodología analítica para determinar el contenido de flavonoides totales en hojas de S. occidentalis, para control de calidad. Los parámetros evaluados fueron: relación fármaco: disolvente, concentración de cloruro de aluminio y tiempo de reacción. La cuantificación de los flavonoides totales se obtuvo tras la reacción con cloruro de aluminio mediante espectrofotometría UV/Vis. Los resultados revelaron que un pico de absorción máximo (λ = 392 nm) fue la cantidad de 0.5 g de una planta cruda en 100 mL de solvente y no hubo influencias significativas entre las concentraciones de cloruro de aluminio o soluciones hidroalcohólicas analizadas. La evaluación del tiempo de reacción permitió caracterizar la cinética como lenta, estabilizándose a partir de 60 min, eligiendo este como tiempo de lectura. El método fue específico, lineal, preciso, exacto y robusto, de acuerdo con las especificaciones establecidas en RDC n. 166/2017. Finalmente, los resultados del estudio mostraron que la metodología medida es simple y precisa y se puede aplicar para la evaluación de la calidad de la especie S. occidentalis.Senna occidentalis Link (Fabaceae), conhecido como “fedegoso”, é utilizado na medicina popular por ser anti-inflamatório, antioxidante, relaxante muscular e inibidor da peroxidação lipídica imputada aos flavonoides contidos em sua composição. A espécie também é constituinte de produtos processados, sendo comercializados em várias partes do mundo inclusive no Brasil, embora existam poucos relatos na literatura sobre o desenvolvimento de um método analítico para quantificação de flavonoides na mesma. O objetivo principal desta pesquisa foi a avaliação de uma metodologia analítica para determinação do teor de flavonoides totais em folhas de S. occidentalis, para controle de qualidade. Os parâmetros avaliados foram: relação fármaco: solvente, concentração de cloreto de alumínio e tempo de reação. A quantificação dos flavonoides totais foi obtida após reação com cloreto de alumínio por espectrofotometria UV/Vis. Os resultados revelaram que um pico máximo de absorção (λ = 392 nm) foi a quantidade de 0,5 g de uma planta crua em 100 mL de solvente e não houve influências significativas entre as concentrações de cloreto de alumínio ou soluções hidroalcóolicas analisadas. A avaliação do tempo de reação permitiu caracterizar a cinética como lenta, estabilizando a partir de 60 min, escolhendo-se este como tempo de leitura. O método foi específico, linear, preciso, exato e robusto, de acordo com as especificações estabelecidas na RDC n. 166/2017. Por fim, os resultados do estudo mostraram que a metodologia de medição é simples e precisa e pode ser aplicada para avaliação da qualidade da espécie S. occidentalis.Research, Society and Development2022-01-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1858410.33448/rsd-v11i1.18584Research, Society and Development; Vol. 11 No. 1; e14411118584Research, Society and Development; Vol. 11 Núm. 1; e14411118584Research, Society and Development; v. 11 n. 1; e144111185842525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/18584/21734Copyright (c) 2022 Vanessa da Silva Luna; Karina Perrelli Randau; Magda Rhayanny Assunção Ferreira; Luiz Alberto Lira Soareshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessLuna, Vanessa da Silva Randau, Karina Perrelli Ferreira, Magda Rhayanny Assunção Soares, Luiz Alberto Lira 2022-01-16T18:08:18Zoai:ojs.pkp.sfu.ca:article/18584Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:38:41.548480Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Development and validation of analytical method by spectrophotometry UV-Vis for quantification of flavonoids in leaves of Senna occidentalis Link Desarrollo y validación de método analítico por espectrofotometría UV-Vis para cuantificación de flavonoides en hojas de Senna occidentalis Link Desenvolvimento e validação de método analítico por espectrofotometria UV-Vis para quantificação de flavonóides em folhas de Senna occidentalis Link |
title |
Development and validation of analytical method by spectrophotometry UV-Vis for quantification of flavonoids in leaves of Senna occidentalis Link |
spellingShingle |
Development and validation of analytical method by spectrophotometry UV-Vis for quantification of flavonoids in leaves of Senna occidentalis Link Luna, Vanessa da Silva Senna occidentalis Flavonóides totais Espectrofotometria ultravioleta-visível Validação. Senna occidentalis Flavonoides totales Espectrofotometría ultravioleta-visible Validación. Senna occidentalis Total flavonoids Ultraviolet-Visible spectrophotometry Validation. |
title_short |
Development and validation of analytical method by spectrophotometry UV-Vis for quantification of flavonoids in leaves of Senna occidentalis Link |
title_full |
Development and validation of analytical method by spectrophotometry UV-Vis for quantification of flavonoids in leaves of Senna occidentalis Link |
title_fullStr |
Development and validation of analytical method by spectrophotometry UV-Vis for quantification of flavonoids in leaves of Senna occidentalis Link |
title_full_unstemmed |
Development and validation of analytical method by spectrophotometry UV-Vis for quantification of flavonoids in leaves of Senna occidentalis Link |
title_sort |
Development and validation of analytical method by spectrophotometry UV-Vis for quantification of flavonoids in leaves of Senna occidentalis Link |
author |
Luna, Vanessa da Silva |
author_facet |
Luna, Vanessa da Silva Randau, Karina Perrelli Ferreira, Magda Rhayanny Assunção Soares, Luiz Alberto Lira |
author_role |
author |
author2 |
Randau, Karina Perrelli Ferreira, Magda Rhayanny Assunção Soares, Luiz Alberto Lira |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Luna, Vanessa da Silva Randau, Karina Perrelli Ferreira, Magda Rhayanny Assunção Soares, Luiz Alberto Lira |
dc.subject.por.fl_str_mv |
Senna occidentalis Flavonóides totais Espectrofotometria ultravioleta-visível Validação. Senna occidentalis Flavonoides totales Espectrofotometría ultravioleta-visible Validación. Senna occidentalis Total flavonoids Ultraviolet-Visible spectrophotometry Validation. |
topic |
Senna occidentalis Flavonóides totais Espectrofotometria ultravioleta-visível Validação. Senna occidentalis Flavonoides totales Espectrofotometría ultravioleta-visible Validación. Senna occidentalis Total flavonoids Ultraviolet-Visible spectrophotometry Validation. |
description |
Senna occidentalis Link (Fabaceae), known as “fedegoso”, is used in folk medicine due to its anti-inflammatory, antioxidant, muscle relaxant and inhibiting lipid peroxidation imputed to flavonoids contained in its composition. The species is also a constituent of processed products are commercialized in various parts of the world including Brazil, although there are few reports in the literature about the development of an analytical method for quantification of flavonoids in it. The main purpose of this research was the evaluation of an analytical methodology to determinate the content of total flavonoids in leaves of S. occidentalis, for quality control. The parameters evaluated were: drug: solvent ratio, concentration of aluminum chloride and reaction time. The quantification of total flavonoids was obtained after reaction with aluminum chloride by UV/Vis spectrophotometry. The results revealed a maximum absorption peak (λ = 392 nm) was the amount of 0.5 g of a plant raw in 100 mL of solvent and there were no significant influences between the concentrations of aluminum chloride or hydroalcoholic solutions analyzed. The evaluation of reaction time allowed to characterize the kinetics as slow, stabilizing from 60 min, choosing this as reading time. The method was specific, linear, precise, accurate and robust, according to the specifications set in RDC n. 166/2017. Finally, the results of the study showed that the measured methodology is simple and accurate and can be applied for quality assessment of the species S. occidentalis. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-03 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/18584 10.33448/rsd-v11i1.18584 |
url |
https://rsdjournal.org/index.php/rsd/article/view/18584 |
identifier_str_mv |
10.33448/rsd-v11i1.18584 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/18584/21734 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 1; e14411118584 Research, Society and Development; Vol. 11 Núm. 1; e14411118584 Research, Society and Development; v. 11 n. 1; e14411118584 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052685312065536 |