Use of recombinant proteins in the diagnosis of sexually transmitted infections: a systematic review and meta-analysis

Detalhes bibliográficos
Autor(a) principal: Silva, Lucas Santana Coelho da
Data de Publicação: 2022
Outros Autores: Castro, Caroline Tianeze de, Gomes, Camila Pachêco, Santos Júnior, Manoel Neres, Barbosa, Maysa Santos, Neves, Wanderson Souza, Ribeiro, Viviane Gomes, Campos, Guilherme Barreto, Bastos, Bruno Lopes, Marques, Lucas Miranda
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/32970
Resumo: The present systematic review and meta-analysis aimed to investigate the use of recombinant proteins to diagnose STIs and evaluate the performance of the tests identified. The databases, SciELO, PubMed, CAPES Journal Portal, and LILACS, were searched according to the PRISMA protocol. A study was considered eligible if it met previously defined criteria. The risk of study bias was assessed according to the QUADAS-2 protocol. The meta-analysis was performed based on the random-effects model, and heterogeneity was quantified using the I² statistic. A total of 1,355 studies were selected, 30 of which were relevant to the following: human immunodeficiency virus (12/30), Treponema pallidum (10/30), Chlamydia trachomatis (3/30), herpes simplex virus (HSV, 2/30), Trichomonas vaginalis (2/30), and HSV/cytomegalovirus (1/30). The main serological tests were ELISA and immunochromatography. The risk of bias was low for most included studies. According to each microorganism, the meta-analysis revealed satisfactory sensitivity and specificity for the analyzed tests. The findings reinforce the relevance of diagnostic tests based on recombinant proteins as viable alternatives for production and inclusion in clinical practice. PROSPERO registration number: CRD42020206331.
id UNIFEI_c8128a865411664647b6431794396816
oai_identifier_str oai:ojs.pkp.sfu.ca:article/32970
network_acronym_str UNIFEI
network_name_str Research, Society and Development
repository_id_str
spelling Use of recombinant proteins in the diagnosis of sexually transmitted infections: a systematic review and meta-analysisUso de proteínas recombinantes en el diagnóstico de infecciones de transmisión sexual: revisión sistemática y metanálisisUso de proteínas recombinantes no diagnóstico de infecções sexualmente transmissíveis: uma revisão sistemática e metanáliseSTIsSystematic reviewMeta-analysisDiagnosis. ITSRevisión sistemáticaMetanálisisDiagnóstico.ISTRevisão sistemáticaMeta-análiseDiagnóstico.The present systematic review and meta-analysis aimed to investigate the use of recombinant proteins to diagnose STIs and evaluate the performance of the tests identified. The databases, SciELO, PubMed, CAPES Journal Portal, and LILACS, were searched according to the PRISMA protocol. A study was considered eligible if it met previously defined criteria. The risk of study bias was assessed according to the QUADAS-2 protocol. The meta-analysis was performed based on the random-effects model, and heterogeneity was quantified using the I² statistic. A total of 1,355 studies were selected, 30 of which were relevant to the following: human immunodeficiency virus (12/30), Treponema pallidum (10/30), Chlamydia trachomatis (3/30), herpes simplex virus (HSV, 2/30), Trichomonas vaginalis (2/30), and HSV/cytomegalovirus (1/30). The main serological tests were ELISA and immunochromatography. The risk of bias was low for most included studies. According to each microorganism, the meta-analysis revealed satisfactory sensitivity and specificity for the analyzed tests. The findings reinforce the relevance of diagnostic tests based on recombinant proteins as viable alternatives for production and inclusion in clinical practice. PROSPERO registration number: CRD42020206331.La presente revisión sistemática y metanálisis tuvo como objetivo investigar el uso de proteínas recombinantes para diagnosticar las ITS y evaluar el rendimiento de las pruebas identificadas. Se realizaron búsquedas en las bases de datos SciELO, PubMed, CAPES Journal Portal y LILACS, según el protocolo PRISMA. Un estudio se consideró elegible si cumplía con los criterios previamente definidos. El riesgo de sesgo del estudio se evaluó según el protocolo QUADAS-2. El metanálisis se realizó con base en el modelo de efectos aleatorios y la heterogeneidad se cuantificó mediante la estadística I². Se seleccionaron un total de 1.355 estudios, 30 de los cuales eran relevantes para lo siguiente: virus de la inmunodeficiencia humana (12/30), Treponema pallidum (10/30), Chlamydia trachomatis (3/30), virus del herpes simple (HSV, 2/ 30), Trichomonas vaginalis (2/30) y HSV/citomegalovirus (1/30). Las principales pruebas serológicas fueron ELISA e inmunocromatografía. El riesgo de sesgo fue bajo para la mayoría de los estudios incluidos. Según cada microorganismo, el metanálisis reveló sensibilidad y especificidad satisfactorias para las pruebas analizadas. Los hallazgos refuerzan la relevancia de las pruebas diagnósticas basadas en proteínas recombinantes como alternativas viables para su producción e inclusión en la práctica clínica. Número de registro PROSPERO: CRD42020206331.A presente revisão sistemática e metanálise teve como objetivo investigar o uso de proteínas recombinantes no diagnóstico de IST e avaliar o desempenho dos testes identificados. As bases de dados SciELO, PubMed, Portal de Periódicos CAPES e LILACS foram pesquisadas de acordo com o protocolo PRISMA. Um estudo foi considerado elegível se atendesse a critérios previamente definidos. O risco de viés do estudo foi avaliado de acordo com o protocolo QUADAS-2. A metanálise foi realizada com base no modelo de efeitos aleatórios e a heterogeneidade foi quantificada pela estatística I². Um total de 1.355 estudos foram selecionados, dos quais 30 eram relevantes para: vírus da imunodeficiência humana (12/30), Treponema pallidum (10/30), Chlamydia trachomatis (3/30), vírus herpes simplex (HSV, 2/ 30), Trichomonas vaginalis (2/30) e HSV/citomegalovírus (1/30). Os principais testes sorológicos foram ELISA e imunocromatografia. O risco de viés foi baixo para a maioria dos estudos incluídos. De acordo com cada microrganismo, a metanálise revelou sensibilidade e especificidade satisfatórias para os testes analisados. Os achados reforçam a relevância dos testes diagnósticos baseados em proteínas recombinantes como alternativas viáveis para produção e inclusão na prática clínica. Número de registro PROSPERO: CRD42020206331.Research, Society and Development2022-08-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3297010.33448/rsd-v11i10.32970Research, Society and Development; Vol. 11 No. 10; e433111032974Research, Society and Development; Vol. 11 Núm. 10; e433111032974Research, Society and Development; v. 11 n. 10; e4331110329742525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/32970/27950Copyright (c) 2022 Lucas Santana Coelho da Silva; Caroline Tianeze de Castro; Camila Pachêco Gomes; Manoel Neres Santos Júnior; Maysa Santos Barbosa; Wanderson Souza Neves; Viviane Gomes Ribeiro; Guilherme Barreto Campos; Bruno Lopes Bastos; Lucas Miranda Marqueshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva, Lucas Santana Coelho daCastro, Caroline Tianeze deGomes, Camila Pachêco Santos Júnior, Manoel Neres Barbosa, Maysa Santos Neves, Wanderson Souza Ribeiro, Viviane Gomes Campos, Guilherme Barreto Bastos, Bruno Lopes Marques, Lucas Miranda 2022-08-12T22:23:03Zoai:ojs.pkp.sfu.ca:article/32970Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:48:46.320739Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Use of recombinant proteins in the diagnosis of sexually transmitted infections: a systematic review and meta-analysis
Uso de proteínas recombinantes en el diagnóstico de infecciones de transmisión sexual: revisión sistemática y metanálisis
Uso de proteínas recombinantes no diagnóstico de infecções sexualmente transmissíveis: uma revisão sistemática e metanálise
title Use of recombinant proteins in the diagnosis of sexually transmitted infections: a systematic review and meta-analysis
spellingShingle Use of recombinant proteins in the diagnosis of sexually transmitted infections: a systematic review and meta-analysis
Silva, Lucas Santana Coelho da
STIs
Systematic review
Meta-analysis
Diagnosis.
ITS
Revisión sistemática
Metanálisis
Diagnóstico.
IST
Revisão sistemática
Meta-análise
Diagnóstico.
title_short Use of recombinant proteins in the diagnosis of sexually transmitted infections: a systematic review and meta-analysis
title_full Use of recombinant proteins in the diagnosis of sexually transmitted infections: a systematic review and meta-analysis
title_fullStr Use of recombinant proteins in the diagnosis of sexually transmitted infections: a systematic review and meta-analysis
title_full_unstemmed Use of recombinant proteins in the diagnosis of sexually transmitted infections: a systematic review and meta-analysis
title_sort Use of recombinant proteins in the diagnosis of sexually transmitted infections: a systematic review and meta-analysis
author Silva, Lucas Santana Coelho da
author_facet Silva, Lucas Santana Coelho da
Castro, Caroline Tianeze de
Gomes, Camila Pachêco
Santos Júnior, Manoel Neres
Barbosa, Maysa Santos
Neves, Wanderson Souza
Ribeiro, Viviane Gomes
Campos, Guilherme Barreto
Bastos, Bruno Lopes
Marques, Lucas Miranda
author_role author
author2 Castro, Caroline Tianeze de
Gomes, Camila Pachêco
Santos Júnior, Manoel Neres
Barbosa, Maysa Santos
Neves, Wanderson Souza
Ribeiro, Viviane Gomes
Campos, Guilherme Barreto
Bastos, Bruno Lopes
Marques, Lucas Miranda
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Lucas Santana Coelho da
Castro, Caroline Tianeze de
Gomes, Camila Pachêco
Santos Júnior, Manoel Neres
Barbosa, Maysa Santos
Neves, Wanderson Souza
Ribeiro, Viviane Gomes
Campos, Guilherme Barreto
Bastos, Bruno Lopes
Marques, Lucas Miranda
dc.subject.por.fl_str_mv STIs
Systematic review
Meta-analysis
Diagnosis.
ITS
Revisión sistemática
Metanálisis
Diagnóstico.
IST
Revisão sistemática
Meta-análise
Diagnóstico.
topic STIs
Systematic review
Meta-analysis
Diagnosis.
ITS
Revisión sistemática
Metanálisis
Diagnóstico.
IST
Revisão sistemática
Meta-análise
Diagnóstico.
description The present systematic review and meta-analysis aimed to investigate the use of recombinant proteins to diagnose STIs and evaluate the performance of the tests identified. The databases, SciELO, PubMed, CAPES Journal Portal, and LILACS, were searched according to the PRISMA protocol. A study was considered eligible if it met previously defined criteria. The risk of study bias was assessed according to the QUADAS-2 protocol. The meta-analysis was performed based on the random-effects model, and heterogeneity was quantified using the I² statistic. A total of 1,355 studies were selected, 30 of which were relevant to the following: human immunodeficiency virus (12/30), Treponema pallidum (10/30), Chlamydia trachomatis (3/30), herpes simplex virus (HSV, 2/30), Trichomonas vaginalis (2/30), and HSV/cytomegalovirus (1/30). The main serological tests were ELISA and immunochromatography. The risk of bias was low for most included studies. According to each microorganism, the meta-analysis revealed satisfactory sensitivity and specificity for the analyzed tests. The findings reinforce the relevance of diagnostic tests based on recombinant proteins as viable alternatives for production and inclusion in clinical practice. PROSPERO registration number: CRD42020206331.
publishDate 2022
dc.date.none.fl_str_mv 2022-08-06
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/32970
10.33448/rsd-v11i10.32970
url https://rsdjournal.org/index.php/rsd/article/view/32970
identifier_str_mv 10.33448/rsd-v11i10.32970
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/32970/27950
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 10; e433111032974
Research, Society and Development; Vol. 11 Núm. 10; e433111032974
Research, Society and Development; v. 11 n. 10; e433111032974
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052797000089600

Registros relacionados