Immunoxpression of MMPs -9, -13 and TIMP-3 after Using Etch-and-rinse Adhesive
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/17315 |
Resumo: | The aim of the present study was to determine the expression of MMP-9, MMP-13 and TIMP-3 after using the XP Bond ™ (Dentsply) dentin-bonding agent on 21 human teeth. Class I deep cavities were prepared and restored. After 01, 07, 14, 21, 30, 90 and 120 days, the teeth were extracted and processed for an immunohistochemical assessment (n=3). Immunohistochemical staining was performed using the monoclonal antibody anti-MMP-9, and polyclonal antibodies anti-MMP-13 and anti-TIMP-3. The immunoreactivity of metalloproteinases 9 and 13 was considered intense and moderate in the first storage intervals, before decreasing over time. In contrast, the immunoreactivity of TIMP-3 was considered absent in dentin and weak in pulp in the first time intervals, and completely absent in the subsequent intervals. MMP-9 expression in dentin and in pulp was prevalent in the dentinal tubules and odontoblastic layer, respectively. MMP-13 expression in dentin and in pulp was predominant in the pre-dentine region and odontoblastic layer, respectively. TIMP-3 expression was absent in dentin and predominantly located in the odontoblastic layer for pulp. In conclusion, the expression patterns of MMP-9, MMP-13 and TIMP-3 altered in the different time intervals studied. The increased expression of TIMP-3 in the first time intervals could suggest a synchronous response to the increased expression of MMPs in the same periods. The results confirmed the low performance of TIMP-3 in the physiological processes of the pulp-dentin complex for the conditions and materials tested herein. |
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Immunoxpression of MMPs -9, -13 and TIMP-3 after Using Etch-and-rinse AdhesiveInmunoexpresión de MMP-9, MMP-13 y TIMP-3 tras el Uso de un Sistema Adhesivo ConvencionalImunoexpressão da MMP-9, MMP-13 e TIMP-3 após o Uso de Sistema Adesivo ConvencionalDentinMatrix Metalloproteinase 9Matrix Metalloproteinase 13Tissue Inhibitor of Metalloproteinase-3Dentin-bonding agents.DentinaMetaloproteinase 9 da MatrizMetaloproteinase 13 da MatrizInibidor Tecidual de Metaloproteinase-3Adesivos dentinários.DentinaMetaloproteinasa 9 de la MatrizMetaloproteinasa 13 de la MatrizInhibidor Tisular de Metaloproteinasa-3Recubrimientos dentinarios.The aim of the present study was to determine the expression of MMP-9, MMP-13 and TIMP-3 after using the XP Bond ™ (Dentsply) dentin-bonding agent on 21 human teeth. Class I deep cavities were prepared and restored. After 01, 07, 14, 21, 30, 90 and 120 days, the teeth were extracted and processed for an immunohistochemical assessment (n=3). Immunohistochemical staining was performed using the monoclonal antibody anti-MMP-9, and polyclonal antibodies anti-MMP-13 and anti-TIMP-3. The immunoreactivity of metalloproteinases 9 and 13 was considered intense and moderate in the first storage intervals, before decreasing over time. In contrast, the immunoreactivity of TIMP-3 was considered absent in dentin and weak in pulp in the first time intervals, and completely absent in the subsequent intervals. MMP-9 expression in dentin and in pulp was prevalent in the dentinal tubules and odontoblastic layer, respectively. MMP-13 expression in dentin and in pulp was predominant in the pre-dentine region and odontoblastic layer, respectively. TIMP-3 expression was absent in dentin and predominantly located in the odontoblastic layer for pulp. In conclusion, the expression patterns of MMP-9, MMP-13 and TIMP-3 altered in the different time intervals studied. The increased expression of TIMP-3 in the first time intervals could suggest a synchronous response to the increased expression of MMPs in the same periods. The results confirmed the low performance of TIMP-3 in the physiological processes of the pulp-dentin complex for the conditions and materials tested herein.El presente estudio tenía como objetivo evaluar la inmunoexpresión de la MMP-9, MMP-13 y TIMP-3 después del uso de sistema adhesivo XP Bond™ (Dentsply) en 21 molares humanos. Cavidades profundas de clase I fueron preparadas y restauradas. Después de 01, 07, 14, 21, 30, 90 y 120 días, los dientes fueron extraídos y procesados para evaluación inmunohistoquímica (n=3). La técnica inmunohistoquímica fue realizada utilizando lo anticuerpo monoclonal anti-MMP-9, y policlonales anti-MMP-13 y anti-TIMP-3. La inmunoexpresión de MMPs 9 y 13 fue considerada intensa y moderada en los intervalos de 01 y 07 días, con menor expresión en los intervalos subsecuentes. En cambio, la inmunoexpresión de TIMP-3 se consideró ausente en dentina y ligera en pulpa en los primeros intervalos de tiempo y ausente en dentina y pulpa en los intervalos posteriores. La expresión de MMP-9 en dentina fue predominante en canales dentinarios y; en pulpa, en odontoblastos. La expresión de MMP-13 en dentina se localizó en la región predentinaria y; en pulpa, en los odontoblastos. La expresión de TIMP-3 estaba ausente en dentina y, en pulpa, se localizaba predominantemente en los odontoblastos. Concluimos que el patrón de expresión de MMP-9, MMP-13 y TIMP-3 está alterado en los diferentes intervalos de tiempo estudiados. El aumento de la expresión de TIMP-3 en los primeros intervalos de tiempo puede demostrar una respuesta al aumento simultáneo de la expresión de las MMPs en los mismos períodos. Los resultados mostraron la baja expresión de TIMP-3 en el complejo dentinopulpar para las condiciones y materiales probados.O objetivo do presente estudo foi avaliar a imunoexpressão da MMP-9, MMP-13 e TIMP-3 após o uso de sistema adesivo XP Bond™ (Dentsply) em 21 molares humanos. Cavidades classe I profundas foram preparadas e restauradas. Depois de 01, 07, 14, 21, 30, 90 e 120 dias, os dentes foram extraídos e processados para avaliação imuno-histoquímica (n=3). A técnica imuno-histoquímica foi realizada utilizando-se o anticorpo monoclonal anti-MMP-9, e policlonais anti-MMP-13 e anti-TIMP-3. A imunoexpressão das MMPs 9 e 13 foi considerada intensa e moderada, respectivamente, nos intervalos de 01 e 07 dias, com menor expressão nos intervalos subsequentes. Em contrapartida, a imunoexpressão do TIMP-3 foi considerada ausente em dentina e leve em polpa nos primeiros intervalos de tempo e ausente em dentina e polpa nos intervalos posteriores. A expressão da MMP-9 em dentina foi predominante nos canalículos dentinários e; em polpa, nos odontoblastos. A expressão da MMP-13 em dentina teve localização predominante na região de pré-dentina e; em polpa, nos odontoblastos. A expressão de TIMP-3 foi ausente em dentina e; em polpa, teve localização predominante nos odontoblastos. Conclui-se que o padrão de expressão das MMP-9, MMP-13 e do TIMP-3 sofre alteração nos diferentes intervalos de tempo estudados. O aumento de expressão do TIMP-3 nos primeiros intervalos de tempo pode demonstrar uma resposta ao simultâneo aumento da expressão das MMPs nos mesmos períodos. Os resultados evidenciaram a pouca expressão do TIMP-3 no complexo dentinopulpar para as condições e materiais testados.Research, Society and Development2021-07-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1731510.33448/rsd-v10i9.17315Research, Society and Development; Vol. 10 No. 9; e14010917315Research, Society and Development; Vol. 10 Núm. 9; e14010917315Research, Society and Development; v. 10 n. 9; e140109173152525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/17315/15832Copyright (c) 2021 Laís Maciel Costa; Ariela Vilela Rizuto; Luciana Sarmento de Mendonça; Marianne de Vasconcelos Carvalho; Márcia Maria Fonseca da Silveira; Ana Paula Veras Sobralhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCosta, Laís Maciel Rizuto, Ariela Vilela Mendonça, Luciana Sarmento deCarvalho, Marianne de Vasconcelos Silveira, Márcia Maria Fonseca da Sobral, Ana Paula Veras 2021-09-12T14:28:06Zoai:ojs.pkp.sfu.ca:article/17315Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:37:41.120066Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Immunoxpression of MMPs -9, -13 and TIMP-3 after Using Etch-and-rinse Adhesive Inmunoexpresión de MMP-9, MMP-13 y TIMP-3 tras el Uso de un Sistema Adhesivo Convencional Imunoexpressão da MMP-9, MMP-13 e TIMP-3 após o Uso de Sistema Adesivo Convencional |
title |
Immunoxpression of MMPs -9, -13 and TIMP-3 after Using Etch-and-rinse Adhesive |
spellingShingle |
Immunoxpression of MMPs -9, -13 and TIMP-3 after Using Etch-and-rinse Adhesive Costa, Laís Maciel Dentin Matrix Metalloproteinase 9 Matrix Metalloproteinase 13 Tissue Inhibitor of Metalloproteinase-3 Dentin-bonding agents. Dentina Metaloproteinase 9 da Matriz Metaloproteinase 13 da Matriz Inibidor Tecidual de Metaloproteinase-3 Adesivos dentinários. Dentina Metaloproteinasa 9 de la Matriz Metaloproteinasa 13 de la Matriz Inhibidor Tisular de Metaloproteinasa-3 Recubrimientos dentinarios. |
title_short |
Immunoxpression of MMPs -9, -13 and TIMP-3 after Using Etch-and-rinse Adhesive |
title_full |
Immunoxpression of MMPs -9, -13 and TIMP-3 after Using Etch-and-rinse Adhesive |
title_fullStr |
Immunoxpression of MMPs -9, -13 and TIMP-3 after Using Etch-and-rinse Adhesive |
title_full_unstemmed |
Immunoxpression of MMPs -9, -13 and TIMP-3 after Using Etch-and-rinse Adhesive |
title_sort |
Immunoxpression of MMPs -9, -13 and TIMP-3 after Using Etch-and-rinse Adhesive |
author |
Costa, Laís Maciel |
author_facet |
Costa, Laís Maciel Rizuto, Ariela Vilela Mendonça, Luciana Sarmento de Carvalho, Marianne de Vasconcelos Silveira, Márcia Maria Fonseca da Sobral, Ana Paula Veras |
author_role |
author |
author2 |
Rizuto, Ariela Vilela Mendonça, Luciana Sarmento de Carvalho, Marianne de Vasconcelos Silveira, Márcia Maria Fonseca da Sobral, Ana Paula Veras |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Costa, Laís Maciel Rizuto, Ariela Vilela Mendonça, Luciana Sarmento de Carvalho, Marianne de Vasconcelos Silveira, Márcia Maria Fonseca da Sobral, Ana Paula Veras |
dc.subject.por.fl_str_mv |
Dentin Matrix Metalloproteinase 9 Matrix Metalloproteinase 13 Tissue Inhibitor of Metalloproteinase-3 Dentin-bonding agents. Dentina Metaloproteinase 9 da Matriz Metaloproteinase 13 da Matriz Inibidor Tecidual de Metaloproteinase-3 Adesivos dentinários. Dentina Metaloproteinasa 9 de la Matriz Metaloproteinasa 13 de la Matriz Inhibidor Tisular de Metaloproteinasa-3 Recubrimientos dentinarios. |
topic |
Dentin Matrix Metalloproteinase 9 Matrix Metalloproteinase 13 Tissue Inhibitor of Metalloproteinase-3 Dentin-bonding agents. Dentina Metaloproteinase 9 da Matriz Metaloproteinase 13 da Matriz Inibidor Tecidual de Metaloproteinase-3 Adesivos dentinários. Dentina Metaloproteinasa 9 de la Matriz Metaloproteinasa 13 de la Matriz Inhibidor Tisular de Metaloproteinasa-3 Recubrimientos dentinarios. |
description |
The aim of the present study was to determine the expression of MMP-9, MMP-13 and TIMP-3 after using the XP Bond ™ (Dentsply) dentin-bonding agent on 21 human teeth. Class I deep cavities were prepared and restored. After 01, 07, 14, 21, 30, 90 and 120 days, the teeth were extracted and processed for an immunohistochemical assessment (n=3). Immunohistochemical staining was performed using the monoclonal antibody anti-MMP-9, and polyclonal antibodies anti-MMP-13 and anti-TIMP-3. The immunoreactivity of metalloproteinases 9 and 13 was considered intense and moderate in the first storage intervals, before decreasing over time. In contrast, the immunoreactivity of TIMP-3 was considered absent in dentin and weak in pulp in the first time intervals, and completely absent in the subsequent intervals. MMP-9 expression in dentin and in pulp was prevalent in the dentinal tubules and odontoblastic layer, respectively. MMP-13 expression in dentin and in pulp was predominant in the pre-dentine region and odontoblastic layer, respectively. TIMP-3 expression was absent in dentin and predominantly located in the odontoblastic layer for pulp. In conclusion, the expression patterns of MMP-9, MMP-13 and TIMP-3 altered in the different time intervals studied. The increased expression of TIMP-3 in the first time intervals could suggest a synchronous response to the increased expression of MMPs in the same periods. The results confirmed the low performance of TIMP-3 in the physiological processes of the pulp-dentin complex for the conditions and materials tested herein. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-19 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/17315 10.33448/rsd-v10i9.17315 |
url |
https://rsdjournal.org/index.php/rsd/article/view/17315 |
identifier_str_mv |
10.33448/rsd-v10i9.17315 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/17315/15832 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 9; e14010917315 Research, Society and Development; Vol. 10 Núm. 9; e14010917315 Research, Society and Development; v. 10 n. 9; e14010917315 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052751594651648 |