Experimental pulmonary paracoccidioidomycosis progression after p2x7 receptor inhibition

Detalhes bibliográficos
Autor(a) principal: Cecatto, Vanessa
Data de Publicação: 2022
Outros Autores: Genske, Rodrigo Daniel, Gandra, Rinaldo Ferreira, Rezende , Mário José de, Loth, Eduardo Alexandre
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/38056
Resumo: Objective: To evaluate the effects of P2X7 receptor inhibition by Brilliant Blue G dye (BBG) in an experimental mode of pulmonary paracoccidioidomycosis. Methodology: Thirty male rats were divided into five groups: absolute control group, negative control group, experimental group 50, experimental group 100, and experimental group 150. The last four groups underwent experimental induction of pulmonary paracoccidioidomycosis; however, only the brilliant blue G experimental groups 50, 100, and 150 were administered BBG intraperitoneally every 48 h at doses of 50, 100, and 150 mg/kg respectively, to inhibit P2X7 receptor activity. To assess the effects of this treatment, lung tissue was analyzed histologically and the levels of the anti-gp43 antibody were measured by enzyme-linked immunosorbent assay. Results and conclusion: Lung biopsies of the animals treated with BBG exhibited a significant reduction in the number and area of granulomas with lower levels of anti-gp43 antibodies relative to those in the untreated group. Treatment with BBG reduced the number and area of granulomas in the lung tissue and levels of anti-gp43 antibodies against Paracoccidioides brasiliensis, thereby mitigating pulmonary paracoccidioidomycosis in the experimental model.
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spelling Experimental pulmonary paracoccidioidomycosis progression after p2x7 receptor inhibitionAnálisis de la evolución patológica de la paracoccidioidomicosis pulmonar experimental tras la inhibición del receptor p2x7Análise da evolução patológica da paracoccidioidomicose pulmonar experimental após inibição do receptor p2x7Paracoccidioides brasiliensisParacoccidioidomicoseMicose pulmonarReceptor Purinérgico P2X7.Paracoccidioides brasiliensisParacoccidioidomicosisEnfermedades Pulmonares FungicasReceptor Purinérgicos P2X7.Paracoccidioides brasiliensisParacoccidioidomycosisDoenças Pulmonares FungicasPurinergic P2X7 Receptor.Objective: To evaluate the effects of P2X7 receptor inhibition by Brilliant Blue G dye (BBG) in an experimental mode of pulmonary paracoccidioidomycosis. Methodology: Thirty male rats were divided into five groups: absolute control group, negative control group, experimental group 50, experimental group 100, and experimental group 150. The last four groups underwent experimental induction of pulmonary paracoccidioidomycosis; however, only the brilliant blue G experimental groups 50, 100, and 150 were administered BBG intraperitoneally every 48 h at doses of 50, 100, and 150 mg/kg respectively, to inhibit P2X7 receptor activity. To assess the effects of this treatment, lung tissue was analyzed histologically and the levels of the anti-gp43 antibody were measured by enzyme-linked immunosorbent assay. Results and conclusion: Lung biopsies of the animals treated with BBG exhibited a significant reduction in the number and area of granulomas with lower levels of anti-gp43 antibodies relative to those in the untreated group. Treatment with BBG reduced the number and area of granulomas in the lung tissue and levels of anti-gp43 antibodies against Paracoccidioides brasiliensis, thereby mitigating pulmonary paracoccidioidomycosis in the experimental model.Objetivo: Evaluar los efectos de la inhibición del receptor P2X7, mediante el uso del colorante Brilliant Blue G, en el modelo experimental de paracoccidioidomicosis pulmonar. Metodología: Treinta ratas macho fueron divididas en cinco grupos: grupo control absoluto, grupo control negativo, grupo experimental 50, grupo experimental 100, grupo experimental 150. A los últimos cuatro grupos se les realizó inducción del modelo experimental de paracoccidioidomicosis pulmonar, sin embargo, solo el Brilliant Blue G de los grupos experimentales 50, 100 y 150 recibieron aplicación intraperitoneal de BBG cada 48 horas, en dosis de 50, 100 y 150 mg/kg, respectivamente, para inhibir la actividad del receptor P2X7. Para analizar los efectos de la terapia propuesta, se evaluó histológicamente el tejido pulmonar de las ratas y se midió el anticuerpo anti-Gp 43 mediante el método ELISA. Resultados: Las biopsias pulmonares de animales tratados con Brilliant Blue G mostraron una disminución significativa en el número y área de granulomas en relación al grupo no tratado y menor título de anticuerpos anti-Gp43 en los grupos que recibieron tratamiento con BBG. Conclusiones: El tratamiento con Azul Brillante G logró reducir el número y área de granulomas en los tejidos pulmonares y los niveles de anticuerpo anti-Pb Gp43, atenuando la Paracoccidioidomicosis pulmonar en el modelo experimental utilizado.Objetivo: Avaliar os efeitos da inibição do receptor P2X7, através da utilização do corante Brilliant Blue G, no modelo experimental de paracoccidioidomicose pulmonar. Metodologia: Foram utilizados 30 ratos machos, distribuídos em cinco grupos: Grupo controle absoluto, Grupo controle negativo, Grupo experimental 50, Grupo experimental 100, grupo experimental 150. Os quatro últimos grupos sofreram indução do modelo experimental de Paracoccidioidomicose pulmonar, porém, apenas os Brilliant Blue G dos grupos experimental 50, 100 e 150 receberam aplicação intraperitoneal de BBG a cada 48 horas, nas doses de 50, 100 e 150 mg/kg respectivamente, para a inibição da atividade do receptor P2X7. Para analisar os efeitos da terapia proposta foi avaliado histologicamente o tecido pulmonar dos ratos e dosado o anticorpo anti-Gp 43 através do método ELISA. Resultados: As biópsias pulmonares dos animais tratados com Brilliant Blue G apresentaram diminuição significativa do número e área de granulomas em relação ao grupo não tratado e menor titulação de anticorpos anti-Gp43 nos grupos que receberam o tratamento com BBG. Conclusões: O tratamento com Brilliant Blue G foi capaz de reduzir o número e área de granulomas nos tecidos pulmonares e os níveis do anticorpo anti-Gp43 de Pb, atenuando a Paracoccidioidomicose pulmonar no modelo experimental utilizado.Research, Society and Development2022-12-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/38056Research, Society and Development; Vol. 11 No. 16; e241111638056Research, Society and Development; Vol. 11 Núm. 16; e241111638056Research, Society and Development; v. 11 n. 16; e2411116380562525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/38056/31593Copyright (c) 2022 Vanessa Cecatto; Rodrigo Daniel Genske; Rinaldo Ferreira Gandra; Mário José de Rezende ; Eduardo Alexandre Lothhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCecatto, VanessaGenske, Rodrigo DanielGandra, Rinaldo Ferreira Rezende , Mário José deLoth, Eduardo Alexandre 2022-12-18T18:26:42Zoai:ojs.pkp.sfu.ca:article/38056Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:51:57.827683Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Experimental pulmonary paracoccidioidomycosis progression after p2x7 receptor inhibition
Análisis de la evolución patológica de la paracoccidioidomicosis pulmonar experimental tras la inhibición del receptor p2x7
Análise da evolução patológica da paracoccidioidomicose pulmonar experimental após inibição do receptor p2x7
title Experimental pulmonary paracoccidioidomycosis progression after p2x7 receptor inhibition
spellingShingle Experimental pulmonary paracoccidioidomycosis progression after p2x7 receptor inhibition
Cecatto, Vanessa
Paracoccidioides brasiliensis
Paracoccidioidomicose
Micose pulmonar
Receptor Purinérgico P2X7.
Paracoccidioides brasiliensis
Paracoccidioidomicosis
Enfermedades Pulmonares Fungicas
Receptor Purinérgicos P2X7.
Paracoccidioides brasiliensis
Paracoccidioidomycosis
Doenças Pulmonares Fungicas
Purinergic P2X7 Receptor.
title_short Experimental pulmonary paracoccidioidomycosis progression after p2x7 receptor inhibition
title_full Experimental pulmonary paracoccidioidomycosis progression after p2x7 receptor inhibition
title_fullStr Experimental pulmonary paracoccidioidomycosis progression after p2x7 receptor inhibition
title_full_unstemmed Experimental pulmonary paracoccidioidomycosis progression after p2x7 receptor inhibition
title_sort Experimental pulmonary paracoccidioidomycosis progression after p2x7 receptor inhibition
author Cecatto, Vanessa
author_facet Cecatto, Vanessa
Genske, Rodrigo Daniel
Gandra, Rinaldo Ferreira
Rezende , Mário José de
Loth, Eduardo Alexandre
author_role author
author2 Genske, Rodrigo Daniel
Gandra, Rinaldo Ferreira
Rezende , Mário José de
Loth, Eduardo Alexandre
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Cecatto, Vanessa
Genske, Rodrigo Daniel
Gandra, Rinaldo Ferreira
Rezende , Mário José de
Loth, Eduardo Alexandre
dc.subject.por.fl_str_mv Paracoccidioides brasiliensis
Paracoccidioidomicose
Micose pulmonar
Receptor Purinérgico P2X7.
Paracoccidioides brasiliensis
Paracoccidioidomicosis
Enfermedades Pulmonares Fungicas
Receptor Purinérgicos P2X7.
Paracoccidioides brasiliensis
Paracoccidioidomycosis
Doenças Pulmonares Fungicas
Purinergic P2X7 Receptor.
topic Paracoccidioides brasiliensis
Paracoccidioidomicose
Micose pulmonar
Receptor Purinérgico P2X7.
Paracoccidioides brasiliensis
Paracoccidioidomicosis
Enfermedades Pulmonares Fungicas
Receptor Purinérgicos P2X7.
Paracoccidioides brasiliensis
Paracoccidioidomycosis
Doenças Pulmonares Fungicas
Purinergic P2X7 Receptor.
description Objective: To evaluate the effects of P2X7 receptor inhibition by Brilliant Blue G dye (BBG) in an experimental mode of pulmonary paracoccidioidomycosis. Methodology: Thirty male rats were divided into five groups: absolute control group, negative control group, experimental group 50, experimental group 100, and experimental group 150. The last four groups underwent experimental induction of pulmonary paracoccidioidomycosis; however, only the brilliant blue G experimental groups 50, 100, and 150 were administered BBG intraperitoneally every 48 h at doses of 50, 100, and 150 mg/kg respectively, to inhibit P2X7 receptor activity. To assess the effects of this treatment, lung tissue was analyzed histologically and the levels of the anti-gp43 antibody were measured by enzyme-linked immunosorbent assay. Results and conclusion: Lung biopsies of the animals treated with BBG exhibited a significant reduction in the number and area of granulomas with lower levels of anti-gp43 antibodies relative to those in the untreated group. Treatment with BBG reduced the number and area of granulomas in the lung tissue and levels of anti-gp43 antibodies against Paracoccidioides brasiliensis, thereby mitigating pulmonary paracoccidioidomycosis in the experimental model.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-06
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/38056
url https://rsdjournal.org/index.php/rsd/article/view/38056
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/38056/31593
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 16; e241111638056
Research, Society and Development; Vol. 11 Núm. 16; e241111638056
Research, Society and Development; v. 11 n. 16; e241111638056
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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