Co-production of polyhydroxyalkanoates and levan by Halomonas smyrnensis AAD6T
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Publication Date: | 2022 |
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Format: | Article |
Language: | eng |
Source: | Research, Society and Development |
Download full: | https://rsdjournal.org/index.php/rsd/article/view/37925 |
Summary: | The simultaneous production of microbial polymers levan and poly[3-hydroxybutyrate] (PHB), a type of polyhydroxyalkanoates, was investigated in this work. The study involved the fermentation of sucrose and molasses by H. smyrnensis AAD6T (BAE2 strain) to produce PHB (intracellular) and levan (extracellular). Both polymers were isolated and characterized by FTIR. Levan was also characterized by thin-layer chromatography (TLC) and viscosimetric analysis. The amount of biomass was 25 g until the end of fermentation. The PHB rate was 0.015 g in both media and the average PHB productivity was 6.0 x 10-4 g PHB/g biomass. The highest rate of levan was 9 g/L in the range of 72–80 h, in the molasses-based medium. The FTIR spectra showed specific signals for each of the polymers, such as the peak at 1700 for the carbonyl group of esters for the PHB and signals at 900 and 800, which are typical signals for levan fructose rings. Furthermore, acid hydrolysis of levan revealed that it was formed only by fructose, as confirmed by TLC With this study, H. smyrnensis AAD6T BAE2 co-produced PHB and levan using a low-cost carbon source, showing great potential in reducing biopolymer manufacturing costs. |
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Co-production of polyhydroxyalkanoates and levan by Halomonas smyrnensis AAD6TCoproducción de polihidroxialcanoatos y levana por Halomonas smyrnensis AAD6TCoprodução de polihidroxialcanoatos e levana por Halomonas smyrnensis AAD6T HalophilesHalomonas smyrnensisPolyhydroxyalkanoatesLevanCo-production.HalófilosHalomonas smyrnensisPolihidroxialcanoatosLevanaCoproducción.HalófilasHalomonas smyrnensisPolihidroxialcanoatosLevanaCoprodução.The simultaneous production of microbial polymers levan and poly[3-hydroxybutyrate] (PHB), a type of polyhydroxyalkanoates, was investigated in this work. The study involved the fermentation of sucrose and molasses by H. smyrnensis AAD6T (BAE2 strain) to produce PHB (intracellular) and levan (extracellular). Both polymers were isolated and characterized by FTIR. Levan was also characterized by thin-layer chromatography (TLC) and viscosimetric analysis. The amount of biomass was 25 g until the end of fermentation. The PHB rate was 0.015 g in both media and the average PHB productivity was 6.0 x 10-4 g PHB/g biomass. The highest rate of levan was 9 g/L in the range of 72–80 h, in the molasses-based medium. The FTIR spectra showed specific signals for each of the polymers, such as the peak at 1700 for the carbonyl group of esters for the PHB and signals at 900 and 800, which are typical signals for levan fructose rings. Furthermore, acid hydrolysis of levan revealed that it was formed only by fructose, as confirmed by TLC With this study, H. smyrnensis AAD6T BAE2 co-produced PHB and levan using a low-cost carbon source, showing great potential in reducing biopolymer manufacturing costs.En este trabajo se investigó la producción simultánea de polímeros microbianos levan y poli[3-hidroxibutirato] (PHB), un tipo de polihidroxialcanoatos. El estudio involucró la fermentación de melaza de sacarosa por H. smyrnensis AAD6T (cepa BAE2) para producir PHB (intracelular) y leván (extracelular). Ambos polímeros fueron aislados y caracterizados por FTIR. Levana también se caracterizó mediante cromatografía en capa fina (CCD) y análisis viscosimétrico. La cantidad de biomasa fue de 25 g hasta el final de la fermentación. La tasa de PHB fue de 0,015 g en ambos medios y la productividad promedio de PHB fue de 6,0 x 10-4 g de PHA/g de biomasa. La tasa más altas de leván fue de 9 g/L en el rango de 72 a 80 h, en el medio a base de melaza. Los espectros FTIR mostraron señales específicas para cada uno de los polímeros, como el pico a 1700 para el grupo carbonilo de ésteres para el PHB y señales a 900 y 800, que son señales típicas para los anillos de fructosa de levano. Además, la hidrólisis ácida del leván reveló que estaba formado únicamente por fructosa, como lo confirmó la CCD. Con este estudio, H. smyrnensis AAD6T BAE2 coprodujo PHB y levan utilizando una fuente de carbono de bajo costo, mostrando un gran potencial para reducir los costos de fabricación de biopolímeros.A produção simultânea dos polímeros microbianos levana e poli[3-hidroxibutirato] (PHB), um tipo de polihidroxialcanoatos, foi investigada neste trabalho. O estudo envolveu a fermentação de sacarose e melaço por H. smyrnensis AAD6T (cepa BAE2) para produzir PHB (intracelular) e levana (extracelular). Ambos os polímeros foram isolados e caracterizados por FTIR. Levana também foi caracterizada por cromatografia em camada delgada (CCD) e análise viscosimétrica. A quantidade de biomassa foi de 25 g até o final da fermentação. A taxa de PHB foi de 0,015 g em ambos os meios e a produtividade média de PHB foi de 6,0 x 10-4 g de PHA/g de biomassa. A maior taxa de levana foi de 9 g/L no intervalo de 72–80 h, no meio à base de melaço. Os espectros de FTIR mostraram sinais específicos para cada um dos polímeros, como o pico em 1700 para o grupo carbonil de ésteres para o PHB e sinais em 900 e 800, que são sinais típicos de anéis de frutose de levana. Além disso, a hidrólise ácida de levana revelou que esta era formada apenas por frutose, como foi confirmada por CCD. Com este estudo, H. smyrnensis AAD6T BAE2 coproduziu PHB e levana usando uma fonte de carbono de baixo custo, mostrando ter um grande potencial na redução de custos de fabricação de biopolímeros.Research, Society and Development2022-12-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3792510.33448/rsd-v11i16.37925Research, Society and Development; Vol. 11 No. 16; e180111637925Research, Society and Development; Vol. 11 Núm. 16; e180111637925Research, Society and Development; v. 11 n. 16; e1801116379252525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/37925/31540Copyright (c) 2022 Edmilson Clarindo de Siqueira; Laureen Michelle Houllouhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSiqueira, Edmilson Clarindo de Houllou, Laureen Michelle 2022-12-18T18:26:42Zoai:ojs.pkp.sfu.ca:article/37925Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:51:53.876167Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Co-production of polyhydroxyalkanoates and levan by Halomonas smyrnensis AAD6T Coproducción de polihidroxialcanoatos y levana por Halomonas smyrnensis AAD6T Coprodução de polihidroxialcanoatos e levana por Halomonas smyrnensis AAD6T |
title |
Co-production of polyhydroxyalkanoates and levan by Halomonas smyrnensis AAD6T |
spellingShingle |
Co-production of polyhydroxyalkanoates and levan by Halomonas smyrnensis AAD6T Siqueira, Edmilson Clarindo de Halophiles Halomonas smyrnensis Polyhydroxyalkanoates Levan Co-production. Halófilos Halomonas smyrnensis Polihidroxialcanoatos Levana Coproducción. Halófilas Halomonas smyrnensis Polihidroxialcanoatos Levana Coprodução. |
title_short |
Co-production of polyhydroxyalkanoates and levan by Halomonas smyrnensis AAD6T |
title_full |
Co-production of polyhydroxyalkanoates and levan by Halomonas smyrnensis AAD6T |
title_fullStr |
Co-production of polyhydroxyalkanoates and levan by Halomonas smyrnensis AAD6T |
title_full_unstemmed |
Co-production of polyhydroxyalkanoates and levan by Halomonas smyrnensis AAD6T |
title_sort |
Co-production of polyhydroxyalkanoates and levan by Halomonas smyrnensis AAD6T |
author |
Siqueira, Edmilson Clarindo de |
author_facet |
Siqueira, Edmilson Clarindo de Houllou, Laureen Michelle |
author_role |
author |
author2 |
Houllou, Laureen Michelle |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Siqueira, Edmilson Clarindo de Houllou, Laureen Michelle |
dc.subject.por.fl_str_mv |
Halophiles Halomonas smyrnensis Polyhydroxyalkanoates Levan Co-production. Halófilos Halomonas smyrnensis Polihidroxialcanoatos Levana Coproducción. Halófilas Halomonas smyrnensis Polihidroxialcanoatos Levana Coprodução. |
topic |
Halophiles Halomonas smyrnensis Polyhydroxyalkanoates Levan Co-production. Halófilos Halomonas smyrnensis Polihidroxialcanoatos Levana Coproducción. Halófilas Halomonas smyrnensis Polihidroxialcanoatos Levana Coprodução. |
description |
The simultaneous production of microbial polymers levan and poly[3-hydroxybutyrate] (PHB), a type of polyhydroxyalkanoates, was investigated in this work. The study involved the fermentation of sucrose and molasses by H. smyrnensis AAD6T (BAE2 strain) to produce PHB (intracellular) and levan (extracellular). Both polymers were isolated and characterized by FTIR. Levan was also characterized by thin-layer chromatography (TLC) and viscosimetric analysis. The amount of biomass was 25 g until the end of fermentation. The PHB rate was 0.015 g in both media and the average PHB productivity was 6.0 x 10-4 g PHB/g biomass. The highest rate of levan was 9 g/L in the range of 72–80 h, in the molasses-based medium. The FTIR spectra showed specific signals for each of the polymers, such as the peak at 1700 for the carbonyl group of esters for the PHB and signals at 900 and 800, which are typical signals for levan fructose rings. Furthermore, acid hydrolysis of levan revealed that it was formed only by fructose, as confirmed by TLC With this study, H. smyrnensis AAD6T BAE2 co-produced PHB and levan using a low-cost carbon source, showing great potential in reducing biopolymer manufacturing costs. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/37925 10.33448/rsd-v11i16.37925 |
url |
https://rsdjournal.org/index.php/rsd/article/view/37925 |
identifier_str_mv |
10.33448/rsd-v11i16.37925 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/37925/31540 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Edmilson Clarindo de Siqueira; Laureen Michelle Houllou https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Edmilson Clarindo de Siqueira; Laureen Michelle Houllou https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 16; e180111637925 Research, Society and Development; Vol. 11 Núm. 16; e180111637925 Research, Society and Development; v. 11 n. 16; e180111637925 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052730224672768 |