Effect of chronic alcoholism on morphology and cell proliferation in salivary glands of Wistar rats

Detalhes bibliográficos
Autor(a) principal: Pinheiro, Nanci Mendes
Data de Publicação: 2022
Outros Autores: Spina, Ana Maria Barros, Silva, Bruna Raphaela Oliveira, Hiss, Lorraine Stephanie, Silveira, Isadora Caixeta, Marson, Jorge Marcelo, Carneiro, Anna Cecília Dias Maciel, Rosa, Rodrigo César, Micheletti, Adilha Misson Rua, Cardoso, Fabrizio Antonio Gomide, Crema, Virginia Oliveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/29619
Resumo: Several disorders which affect the oral cavity, salivary composition and, flow have been described in alcoholics chronic, thus this study aimed to evaluate the effects of chronic alcoholism on the salivary glands of Wistar rats. It was used 18 animals randomly subdivided into two groups: control (C) and treated with ethanol (T). C group (n=9) received water ad libitum and T group (n=9) received alcoholic solution ad libitum: 5% ethanol (1st week), 10% ethanol (2nd week) and 20% ethanol (for 9 weeks). After the treatment period, the animals were anesthetized and euthanized by decapitation. The parotid, submandibular and sublingual salivary glands of both groups were dissected, fixed and processed for paraffin. Histological sections were stained with hematoxylin/eosin and Picro-Sirius red stained, and the rate of cell proliferation was determined by immunohistochemistry with the marker KI-67. The salivary glands of group C showed preserved parenchyma, while the parotid and submandibular glands of group T showed hydropic degeneration, atypical nuclei, non-evident nucleolus and condensed chromatin in serous acini. The intercalated, striated and excretory ducts were seen with punctual areas of degeneration and nuclear atypia. In the submandibular glands there was a 73% increase in proliferative cells in T, when compared to C (p<0.05). However, in the parotid glands, there was no significant difference in the rate of cell proliferation. The results suggest that there is an increase in the rate of cell proliferation in submandibular glands of Wistar rats subjected to chronic alcohol consumption, and also show that chronic alcohol consumption promotes morphological changes in the salivary glands of these rats, causing a deleterious effect on cellular structures.
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spelling Effect of chronic alcoholism on morphology and cell proliferation in salivary glands of Wistar ratsEfecto del alcoholismo crónico sobre la morfología y proliferación celular en glándulas salivales de ratas WistarEfeito do alcoolismo crônico sobre a morfologia e a proliferação celular em glândulas salivares de ratos WistarGlândulas salivaresAlcoolismoGlândula parótidaGlândula sublingualGlândula submandibular.Salivary glandAlcoholismParotid glandSublingual glandSubmandibular gland.Glándulas salivalesAlcoholismoGlándula parótidaGlándula sublingualGlándula submandibular.Several disorders which affect the oral cavity, salivary composition and, flow have been described in alcoholics chronic, thus this study aimed to evaluate the effects of chronic alcoholism on the salivary glands of Wistar rats. It was used 18 animals randomly subdivided into two groups: control (C) and treated with ethanol (T). C group (n=9) received water ad libitum and T group (n=9) received alcoholic solution ad libitum: 5% ethanol (1st week), 10% ethanol (2nd week) and 20% ethanol (for 9 weeks). After the treatment period, the animals were anesthetized and euthanized by decapitation. The parotid, submandibular and sublingual salivary glands of both groups were dissected, fixed and processed for paraffin. Histological sections were stained with hematoxylin/eosin and Picro-Sirius red stained, and the rate of cell proliferation was determined by immunohistochemistry with the marker KI-67. The salivary glands of group C showed preserved parenchyma, while the parotid and submandibular glands of group T showed hydropic degeneration, atypical nuclei, non-evident nucleolus and condensed chromatin in serous acini. The intercalated, striated and excretory ducts were seen with punctual areas of degeneration and nuclear atypia. In the submandibular glands there was a 73% increase in proliferative cells in T, when compared to C (p<0.05). However, in the parotid glands, there was no significant difference in the rate of cell proliferation. The results suggest that there is an increase in the rate of cell proliferation in submandibular glands of Wistar rats subjected to chronic alcohol consumption, and also show that chronic alcohol consumption promotes morphological changes in the salivary glands of these rats, causing a deleterious effect on cellular structures.En los alcohólicos crónicos se describen varios trastornos que afectan la cavidad oral y la composición y flujo salival. Así, este estudio tuvo como objetivo evaluar los efectos del consumo de alcohol enfermedad crónica en las glándulas salivales de ratas Wistar. Dieciocho animales se dividieron aleatoriamente en dos grupos: control (C) y tratados con etanol (T). El grupo C (n=9) recibió agua ad libitum y el grupo T (n=9) recibió solución alcohólica ad libitum: etanol al 5% (1ª semana), etanol al 10% (2ª semana) y etanol al 20% (durante nueve semanas). Después del período de tratamiento, los animales fueron anestesiados y sacrificados por decapitación. las glándulas Se disecaron glándulas salivales parótidas, submaxilares y sublinguales de ambos grupos, fijado y transformado en parafina. Las secciones histológicas se tiñeron con hematoxilina/eosina y picrosirius, y la tasa de proliferación celular se determinó mediante inmunohistoquímica con el marcador KI-67. Las glándulas salivales del grupo C presentaban parénquima preservado, mientras que las glándulas parótidas y submandibulares del grupo T presentaban degeneración hidrópica, núcleos atípicos, nucléolo no evidente y cromatina condensada en acinos serosos. Los conductos intercalados, estriados y excretores se observaron con áreas puntuales de degeneración y atipia nuclear. En las glándulas submandibulares hubo un aumento del 73% de células proliferativas en T, en comparación con C (p<0,05). Sin embargo, en las glándulas parótidas no hubo diferencia significativa en la tasa de proliferación celular. Los resultados sugieren que existe un aumento en la tasa de proliferación celular en las glándulas submandibulares de ratas Wistar sometidas al consumo crónico de alcohol, y también muestran que el consumo crónico de alcohol promueve cambios morfológicos en las glándulas salivales de estas ratas, provocando un efecto deletéreo en la función celular estructuras.Em alcoólatras crônicos são descritos vários distúrbios que afetam a cavidade oral e a composição e o fluxo salivar. Desta forma, este estudo visou avaliar os efeitos do etilismo crônico sobre as glândulas salivares de ratos Wistar. Foram utilizados 18 animais subdivididos aleatoriamente em dois grupos: controle (C) e tratados com etanol (T). O grupo C (n=9) recebeu água ad libitum e o T (n=9) recebeu solução alcoólica ad libitum: etanol 5% (1ª semana), etanol 10% (2a semana) e etanol 20% (por nove semanas). Após o período de tratamento, os animais foram anestesiados e sacrificados por decapitação. As glândulas salivares parótidas, submandibulares e sublinguais de ambos os grupos foram dissecadas, fixadas e processadas para parafina. Os cortes histológicos foram corados com hematoxilina/eosina e picro-sirius, e a taxa de proliferação celular foi determinada por imuno-histoquímica com o marcador KI-67. As glândulas salivares do grupo C apresentaram o parênquima preservado, ao passo que as glândulas parótidas e submandibulares do grupo T apresentaram degeneração hidrópica, núcleos atípicos, nucléolo não evidente e cromatina condensada nos ácinos serosos. Os ductos intercalares, estriados e excretores foram vistos com áreas pontuais de degeneração e atipia nuclear. Nas glândulas submandibulares houve um aumento de 73% de células proliferativas em T, quando comparado com C (p<0.05). Contudo, nas glândulas parótidas, não houve diferença significativa na taxa de proliferação celular. Os resultados sugerem que ocorre aumento da taxa de proliferação celular em glândulas submandibulares de ratos Wistar submetidos ao consumo crônico de álcool, e também mostram que o etilismo crônico promove alterações morfológicas nas glândulas salivares destes ratos, causando um efeito deletério nas estruturas celulares.Research, Society and Development2022-05-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2961910.33448/rsd-v11i7.29619Research, Society and Development; Vol. 11 No. 7; e13211729619Research, Society and Development; Vol. 11 Núm. 7; e13211729619Research, Society and Development; v. 11 n. 7; e132117296192525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/29619/25707Copyright (c) 2022 Nanci Mendes Pinheiro; Ana Maria Barros Spina; Bruna Raphaela Oliveira Silva; Lorraine Stephanie Hiss; Isadora Caixeta Silveira; Jorge Marcelo Marson; Anna Cecília Dias Maciel Carneiro; Rodrigo César Rosa; Adilha Misson Rua Micheletti; Fabrizio Antonio Gomide Cardoso; Virginia Oliveira Cremahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessPinheiro, Nanci Mendes Spina, Ana Maria Barros Silva, Bruna Raphaela Oliveira Hiss, Lorraine Stephanie Silveira, Isadora CaixetaMarson, Jorge MarceloCarneiro, Anna Cecília Dias Maciel Rosa, Rodrigo César Micheletti, Adilha Misson Rua Cardoso, Fabrizio Antonio GomideCrema, Virginia Oliveira2022-06-06T15:12:05Zoai:ojs.pkp.sfu.ca:article/29619Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:46:38.671593Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Effect of chronic alcoholism on morphology and cell proliferation in salivary glands of Wistar rats
Efecto del alcoholismo crónico sobre la morfología y proliferación celular en glándulas salivales de ratas Wistar
Efeito do alcoolismo crônico sobre a morfologia e a proliferação celular em glândulas salivares de ratos Wistar
title Effect of chronic alcoholism on morphology and cell proliferation in salivary glands of Wistar rats
spellingShingle Effect of chronic alcoholism on morphology and cell proliferation in salivary glands of Wistar rats
Pinheiro, Nanci Mendes
Glândulas salivares
Alcoolismo
Glândula parótida
Glândula sublingual
Glândula submandibular.
Salivary gland
Alcoholism
Parotid gland
Sublingual gland
Submandibular gland.
Glándulas salivales
Alcoholismo
Glándula parótida
Glándula sublingual
Glándula submandibular.
title_short Effect of chronic alcoholism on morphology and cell proliferation in salivary glands of Wistar rats
title_full Effect of chronic alcoholism on morphology and cell proliferation in salivary glands of Wistar rats
title_fullStr Effect of chronic alcoholism on morphology and cell proliferation in salivary glands of Wistar rats
title_full_unstemmed Effect of chronic alcoholism on morphology and cell proliferation in salivary glands of Wistar rats
title_sort Effect of chronic alcoholism on morphology and cell proliferation in salivary glands of Wistar rats
author Pinheiro, Nanci Mendes
author_facet Pinheiro, Nanci Mendes
Spina, Ana Maria Barros
Silva, Bruna Raphaela Oliveira
Hiss, Lorraine Stephanie
Silveira, Isadora Caixeta
Marson, Jorge Marcelo
Carneiro, Anna Cecília Dias Maciel
Rosa, Rodrigo César
Micheletti, Adilha Misson Rua
Cardoso, Fabrizio Antonio Gomide
Crema, Virginia Oliveira
author_role author
author2 Spina, Ana Maria Barros
Silva, Bruna Raphaela Oliveira
Hiss, Lorraine Stephanie
Silveira, Isadora Caixeta
Marson, Jorge Marcelo
Carneiro, Anna Cecília Dias Maciel
Rosa, Rodrigo César
Micheletti, Adilha Misson Rua
Cardoso, Fabrizio Antonio Gomide
Crema, Virginia Oliveira
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pinheiro, Nanci Mendes
Spina, Ana Maria Barros
Silva, Bruna Raphaela Oliveira
Hiss, Lorraine Stephanie
Silveira, Isadora Caixeta
Marson, Jorge Marcelo
Carneiro, Anna Cecília Dias Maciel
Rosa, Rodrigo César
Micheletti, Adilha Misson Rua
Cardoso, Fabrizio Antonio Gomide
Crema, Virginia Oliveira
dc.subject.por.fl_str_mv Glândulas salivares
Alcoolismo
Glândula parótida
Glândula sublingual
Glândula submandibular.
Salivary gland
Alcoholism
Parotid gland
Sublingual gland
Submandibular gland.
Glándulas salivales
Alcoholismo
Glándula parótida
Glándula sublingual
Glándula submandibular.
topic Glândulas salivares
Alcoolismo
Glândula parótida
Glândula sublingual
Glândula submandibular.
Salivary gland
Alcoholism
Parotid gland
Sublingual gland
Submandibular gland.
Glándulas salivales
Alcoholismo
Glándula parótida
Glándula sublingual
Glándula submandibular.
description Several disorders which affect the oral cavity, salivary composition and, flow have been described in alcoholics chronic, thus this study aimed to evaluate the effects of chronic alcoholism on the salivary glands of Wistar rats. It was used 18 animals randomly subdivided into two groups: control (C) and treated with ethanol (T). C group (n=9) received water ad libitum and T group (n=9) received alcoholic solution ad libitum: 5% ethanol (1st week), 10% ethanol (2nd week) and 20% ethanol (for 9 weeks). After the treatment period, the animals were anesthetized and euthanized by decapitation. The parotid, submandibular and sublingual salivary glands of both groups were dissected, fixed and processed for paraffin. Histological sections were stained with hematoxylin/eosin and Picro-Sirius red stained, and the rate of cell proliferation was determined by immunohistochemistry with the marker KI-67. The salivary glands of group C showed preserved parenchyma, while the parotid and submandibular glands of group T showed hydropic degeneration, atypical nuclei, non-evident nucleolus and condensed chromatin in serous acini. The intercalated, striated and excretory ducts were seen with punctual areas of degeneration and nuclear atypia. In the submandibular glands there was a 73% increase in proliferative cells in T, when compared to C (p<0.05). However, in the parotid glands, there was no significant difference in the rate of cell proliferation. The results suggest that there is an increase in the rate of cell proliferation in submandibular glands of Wistar rats subjected to chronic alcohol consumption, and also show that chronic alcohol consumption promotes morphological changes in the salivary glands of these rats, causing a deleterious effect on cellular structures.
publishDate 2022
dc.date.none.fl_str_mv 2022-05-18
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/29619
10.33448/rsd-v11i7.29619
url https://rsdjournal.org/index.php/rsd/article/view/29619
identifier_str_mv 10.33448/rsd-v11i7.29619
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/29619/25707
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 7; e13211729619
Research, Society and Development; Vol. 11 Núm. 7; e13211729619
Research, Society and Development; v. 11 n. 7; e13211729619
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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