Effect of chronic alcoholism on morphology and cell proliferation in salivary glands of Wistar rats
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/29619 |
Resumo: | Several disorders which affect the oral cavity, salivary composition and, flow have been described in alcoholics chronic, thus this study aimed to evaluate the effects of chronic alcoholism on the salivary glands of Wistar rats. It was used 18 animals randomly subdivided into two groups: control (C) and treated with ethanol (T). C group (n=9) received water ad libitum and T group (n=9) received alcoholic solution ad libitum: 5% ethanol (1st week), 10% ethanol (2nd week) and 20% ethanol (for 9 weeks). After the treatment period, the animals were anesthetized and euthanized by decapitation. The parotid, submandibular and sublingual salivary glands of both groups were dissected, fixed and processed for paraffin. Histological sections were stained with hematoxylin/eosin and Picro-Sirius red stained, and the rate of cell proliferation was determined by immunohistochemistry with the marker KI-67. The salivary glands of group C showed preserved parenchyma, while the parotid and submandibular glands of group T showed hydropic degeneration, atypical nuclei, non-evident nucleolus and condensed chromatin in serous acini. The intercalated, striated and excretory ducts were seen with punctual areas of degeneration and nuclear atypia. In the submandibular glands there was a 73% increase in proliferative cells in T, when compared to C (p<0.05). However, in the parotid glands, there was no significant difference in the rate of cell proliferation. The results suggest that there is an increase in the rate of cell proliferation in submandibular glands of Wistar rats subjected to chronic alcohol consumption, and also show that chronic alcohol consumption promotes morphological changes in the salivary glands of these rats, causing a deleterious effect on cellular structures. |
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Effect of chronic alcoholism on morphology and cell proliferation in salivary glands of Wistar ratsEfecto del alcoholismo crónico sobre la morfología y proliferación celular en glándulas salivales de ratas WistarEfeito do alcoolismo crônico sobre a morfologia e a proliferação celular em glândulas salivares de ratos WistarGlândulas salivaresAlcoolismoGlândula parótidaGlândula sublingualGlândula submandibular.Salivary glandAlcoholismParotid glandSublingual glandSubmandibular gland.Glándulas salivalesAlcoholismoGlándula parótidaGlándula sublingualGlándula submandibular.Several disorders which affect the oral cavity, salivary composition and, flow have been described in alcoholics chronic, thus this study aimed to evaluate the effects of chronic alcoholism on the salivary glands of Wistar rats. It was used 18 animals randomly subdivided into two groups: control (C) and treated with ethanol (T). C group (n=9) received water ad libitum and T group (n=9) received alcoholic solution ad libitum: 5% ethanol (1st week), 10% ethanol (2nd week) and 20% ethanol (for 9 weeks). After the treatment period, the animals were anesthetized and euthanized by decapitation. The parotid, submandibular and sublingual salivary glands of both groups were dissected, fixed and processed for paraffin. Histological sections were stained with hematoxylin/eosin and Picro-Sirius red stained, and the rate of cell proliferation was determined by immunohistochemistry with the marker KI-67. The salivary glands of group C showed preserved parenchyma, while the parotid and submandibular glands of group T showed hydropic degeneration, atypical nuclei, non-evident nucleolus and condensed chromatin in serous acini. The intercalated, striated and excretory ducts were seen with punctual areas of degeneration and nuclear atypia. In the submandibular glands there was a 73% increase in proliferative cells in T, when compared to C (p<0.05). However, in the parotid glands, there was no significant difference in the rate of cell proliferation. The results suggest that there is an increase in the rate of cell proliferation in submandibular glands of Wistar rats subjected to chronic alcohol consumption, and also show that chronic alcohol consumption promotes morphological changes in the salivary glands of these rats, causing a deleterious effect on cellular structures.En los alcohólicos crónicos se describen varios trastornos que afectan la cavidad oral y la composición y flujo salival. Así, este estudio tuvo como objetivo evaluar los efectos del consumo de alcohol enfermedad crónica en las glándulas salivales de ratas Wistar. Dieciocho animales se dividieron aleatoriamente en dos grupos: control (C) y tratados con etanol (T). El grupo C (n=9) recibió agua ad libitum y el grupo T (n=9) recibió solución alcohólica ad libitum: etanol al 5% (1ª semana), etanol al 10% (2ª semana) y etanol al 20% (durante nueve semanas). Después del período de tratamiento, los animales fueron anestesiados y sacrificados por decapitación. las glándulas Se disecaron glándulas salivales parótidas, submaxilares y sublinguales de ambos grupos, fijado y transformado en parafina. Las secciones histológicas se tiñeron con hematoxilina/eosina y picrosirius, y la tasa de proliferación celular se determinó mediante inmunohistoquímica con el marcador KI-67. Las glándulas salivales del grupo C presentaban parénquima preservado, mientras que las glándulas parótidas y submandibulares del grupo T presentaban degeneración hidrópica, núcleos atípicos, nucléolo no evidente y cromatina condensada en acinos serosos. Los conductos intercalados, estriados y excretores se observaron con áreas puntuales de degeneración y atipia nuclear. En las glándulas submandibulares hubo un aumento del 73% de células proliferativas en T, en comparación con C (p<0,05). Sin embargo, en las glándulas parótidas no hubo diferencia significativa en la tasa de proliferación celular. Los resultados sugieren que existe un aumento en la tasa de proliferación celular en las glándulas submandibulares de ratas Wistar sometidas al consumo crónico de alcohol, y también muestran que el consumo crónico de alcohol promueve cambios morfológicos en las glándulas salivales de estas ratas, provocando un efecto deletéreo en la función celular estructuras.Em alcoólatras crônicos são descritos vários distúrbios que afetam a cavidade oral e a composição e o fluxo salivar. Desta forma, este estudo visou avaliar os efeitos do etilismo crônico sobre as glândulas salivares de ratos Wistar. Foram utilizados 18 animais subdivididos aleatoriamente em dois grupos: controle (C) e tratados com etanol (T). O grupo C (n=9) recebeu água ad libitum e o T (n=9) recebeu solução alcoólica ad libitum: etanol 5% (1ª semana), etanol 10% (2a semana) e etanol 20% (por nove semanas). Após o período de tratamento, os animais foram anestesiados e sacrificados por decapitação. As glândulas salivares parótidas, submandibulares e sublinguais de ambos os grupos foram dissecadas, fixadas e processadas para parafina. Os cortes histológicos foram corados com hematoxilina/eosina e picro-sirius, e a taxa de proliferação celular foi determinada por imuno-histoquímica com o marcador KI-67. As glândulas salivares do grupo C apresentaram o parênquima preservado, ao passo que as glândulas parótidas e submandibulares do grupo T apresentaram degeneração hidrópica, núcleos atípicos, nucléolo não evidente e cromatina condensada nos ácinos serosos. Os ductos intercalares, estriados e excretores foram vistos com áreas pontuais de degeneração e atipia nuclear. Nas glândulas submandibulares houve um aumento de 73% de células proliferativas em T, quando comparado com C (p<0.05). Contudo, nas glândulas parótidas, não houve diferença significativa na taxa de proliferação celular. Os resultados sugerem que ocorre aumento da taxa de proliferação celular em glândulas submandibulares de ratos Wistar submetidos ao consumo crônico de álcool, e também mostram que o etilismo crônico promove alterações morfológicas nas glândulas salivares destes ratos, causando um efeito deletério nas estruturas celulares.Research, Society and Development2022-05-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2961910.33448/rsd-v11i7.29619Research, Society and Development; Vol. 11 No. 7; e13211729619Research, Society and Development; Vol. 11 Núm. 7; e13211729619Research, Society and Development; v. 11 n. 7; e132117296192525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/29619/25707Copyright (c) 2022 Nanci Mendes Pinheiro; Ana Maria Barros Spina; Bruna Raphaela Oliveira Silva; Lorraine Stephanie Hiss; Isadora Caixeta Silveira; Jorge Marcelo Marson; Anna Cecília Dias Maciel Carneiro; Rodrigo César Rosa; Adilha Misson Rua Micheletti; Fabrizio Antonio Gomide Cardoso; Virginia Oliveira Cremahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessPinheiro, Nanci Mendes Spina, Ana Maria Barros Silva, Bruna Raphaela Oliveira Hiss, Lorraine Stephanie Silveira, Isadora CaixetaMarson, Jorge MarceloCarneiro, Anna Cecília Dias Maciel Rosa, Rodrigo César Micheletti, Adilha Misson Rua Cardoso, Fabrizio Antonio GomideCrema, Virginia Oliveira2022-06-06T15:12:05Zoai:ojs.pkp.sfu.ca:article/29619Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:46:38.671593Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Effect of chronic alcoholism on morphology and cell proliferation in salivary glands of Wistar rats Efecto del alcoholismo crónico sobre la morfología y proliferación celular en glándulas salivales de ratas Wistar Efeito do alcoolismo crônico sobre a morfologia e a proliferação celular em glândulas salivares de ratos Wistar |
title |
Effect of chronic alcoholism on morphology and cell proliferation in salivary glands of Wistar rats |
spellingShingle |
Effect of chronic alcoholism on morphology and cell proliferation in salivary glands of Wistar rats Pinheiro, Nanci Mendes Glândulas salivares Alcoolismo Glândula parótida Glândula sublingual Glândula submandibular. Salivary gland Alcoholism Parotid gland Sublingual gland Submandibular gland. Glándulas salivales Alcoholismo Glándula parótida Glándula sublingual Glándula submandibular. |
title_short |
Effect of chronic alcoholism on morphology and cell proliferation in salivary glands of Wistar rats |
title_full |
Effect of chronic alcoholism on morphology and cell proliferation in salivary glands of Wistar rats |
title_fullStr |
Effect of chronic alcoholism on morphology and cell proliferation in salivary glands of Wistar rats |
title_full_unstemmed |
Effect of chronic alcoholism on morphology and cell proliferation in salivary glands of Wistar rats |
title_sort |
Effect of chronic alcoholism on morphology and cell proliferation in salivary glands of Wistar rats |
author |
Pinheiro, Nanci Mendes |
author_facet |
Pinheiro, Nanci Mendes Spina, Ana Maria Barros Silva, Bruna Raphaela Oliveira Hiss, Lorraine Stephanie Silveira, Isadora Caixeta Marson, Jorge Marcelo Carneiro, Anna Cecília Dias Maciel Rosa, Rodrigo César Micheletti, Adilha Misson Rua Cardoso, Fabrizio Antonio Gomide Crema, Virginia Oliveira |
author_role |
author |
author2 |
Spina, Ana Maria Barros Silva, Bruna Raphaela Oliveira Hiss, Lorraine Stephanie Silveira, Isadora Caixeta Marson, Jorge Marcelo Carneiro, Anna Cecília Dias Maciel Rosa, Rodrigo César Micheletti, Adilha Misson Rua Cardoso, Fabrizio Antonio Gomide Crema, Virginia Oliveira |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Pinheiro, Nanci Mendes Spina, Ana Maria Barros Silva, Bruna Raphaela Oliveira Hiss, Lorraine Stephanie Silveira, Isadora Caixeta Marson, Jorge Marcelo Carneiro, Anna Cecília Dias Maciel Rosa, Rodrigo César Micheletti, Adilha Misson Rua Cardoso, Fabrizio Antonio Gomide Crema, Virginia Oliveira |
dc.subject.por.fl_str_mv |
Glândulas salivares Alcoolismo Glândula parótida Glândula sublingual Glândula submandibular. Salivary gland Alcoholism Parotid gland Sublingual gland Submandibular gland. Glándulas salivales Alcoholismo Glándula parótida Glándula sublingual Glándula submandibular. |
topic |
Glândulas salivares Alcoolismo Glândula parótida Glândula sublingual Glândula submandibular. Salivary gland Alcoholism Parotid gland Sublingual gland Submandibular gland. Glándulas salivales Alcoholismo Glándula parótida Glándula sublingual Glándula submandibular. |
description |
Several disorders which affect the oral cavity, salivary composition and, flow have been described in alcoholics chronic, thus this study aimed to evaluate the effects of chronic alcoholism on the salivary glands of Wistar rats. It was used 18 animals randomly subdivided into two groups: control (C) and treated with ethanol (T). C group (n=9) received water ad libitum and T group (n=9) received alcoholic solution ad libitum: 5% ethanol (1st week), 10% ethanol (2nd week) and 20% ethanol (for 9 weeks). After the treatment period, the animals were anesthetized and euthanized by decapitation. The parotid, submandibular and sublingual salivary glands of both groups were dissected, fixed and processed for paraffin. Histological sections were stained with hematoxylin/eosin and Picro-Sirius red stained, and the rate of cell proliferation was determined by immunohistochemistry with the marker KI-67. The salivary glands of group C showed preserved parenchyma, while the parotid and submandibular glands of group T showed hydropic degeneration, atypical nuclei, non-evident nucleolus and condensed chromatin in serous acini. The intercalated, striated and excretory ducts were seen with punctual areas of degeneration and nuclear atypia. In the submandibular glands there was a 73% increase in proliferative cells in T, when compared to C (p<0.05). However, in the parotid glands, there was no significant difference in the rate of cell proliferation. The results suggest that there is an increase in the rate of cell proliferation in submandibular glands of Wistar rats subjected to chronic alcohol consumption, and also show that chronic alcohol consumption promotes morphological changes in the salivary glands of these rats, causing a deleterious effect on cellular structures. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-18 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/29619 10.33448/rsd-v11i7.29619 |
url |
https://rsdjournal.org/index.php/rsd/article/view/29619 |
identifier_str_mv |
10.33448/rsd-v11i7.29619 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/29619/25707 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 7; e13211729619 Research, Society and Development; Vol. 11 Núm. 7; e13211729619 Research, Society and Development; v. 11 n. 7; e13211729619 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052712541487104 |