Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases

Detalhes bibliográficos
Autor(a) principal: Ferreira, Jardel Fábio Lopes
Data de Publicação: 2020
Outros Autores: Amorim, Murilo Tavares, Franco Neto, Walter Félix, Luna, Francisco Canindé Ferreira de, Melo, Karla Fabiane Lopes de, Borges, Gleissy Adriane Lima, Araújo, Ana Paula Sousa, Holanda, Gustavo Moraes, Casseb, Samir Mansour Moraes, Cruz, Ana Cecília Ribeiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
DOI: 10.33448/rsd-v9i12.10699
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/10699
Resumo: Objective: Evaluate the miRNA-15 expression profile involved in cellular apoptotic regulation factors. Methodology: We used the H818308 Asian strain of ZIKV without neurological damage. The inoculations occurred in human embryonic kidney cells (HEK-293). After inoculation, samples were extracted for RT-qPCR quantification of viral RNA and miR-15. The level of activation of caspases 1, 3/7 and 8 of cells was performed using chemofluorescence. Results: The ZIKV infection alters the expression of genes and their regulators, affecting several cellular physiological processes such as apoptosis. Conclusion: Therefore, it is important to emphasize that renal progenitor cells (HEK-293) are susceptible to VZIK infection. The genetic deregulation resulting from infection directly affects important cellular processes such as apoptosis from the disordered miRNA-15 expression during the infection period.
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spelling Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspasesLa infección por el Vírus Zika en células humanas altera el perfil de expresión del miRNA-15 y la activación de caspasas apoptóticasInfecção por Zika Vírus em células humanas altera o perfil de expressão de miRNA-15 e ativação de caspases apoptóticasVirus zikaApoptosemRNA-15HEK-293.Virus zikaApoptosismiRNA-15HEK-293.Zika virusApoptosismiRNA-15HEK-293.Objective: Evaluate the miRNA-15 expression profile involved in cellular apoptotic regulation factors. Methodology: We used the H818308 Asian strain of ZIKV without neurological damage. The inoculations occurred in human embryonic kidney cells (HEK-293). After inoculation, samples were extracted for RT-qPCR quantification of viral RNA and miR-15. The level of activation of caspases 1, 3/7 and 8 of cells was performed using chemofluorescence. Results: The ZIKV infection alters the expression of genes and their regulators, affecting several cellular physiological processes such as apoptosis. Conclusion: Therefore, it is important to emphasize that renal progenitor cells (HEK-293) are susceptible to VZIK infection. The genetic deregulation resulting from infection directly affects important cellular processes such as apoptosis from the disordered miRNA-15 expression during the infection period.Objetivo: Evaluar el perfil de expresión de miARN-15 implicado en factores de regulación apoptótica celular. Metodología: Utilizamos la cepa asiática H818308 de ZIKV sin daño neurológico. Las inoculaciones se produjeron en células renales embrionarias humanas (HEK-293). Después de la inoculación, se extrajeron muestras para la cuantificación RT-qPCR de ARN viral y miR-15. El nivel de activación de las caspasas 1, 3/7 y 8 de las células se realizó mediante quimiofluorescencia. Resultados: La infección por ZIKV altera la expresión de genes y sus reguladores, afectando varios procesos fisiológicos celulares como la apoptosis. Conclusión: Por lo tanto, es importante enfatizar que las células progenitoras renales (HEK-293) son susceptibles a la infección por VZIK. La desregulación genética resultante de la infección afecta directamente a importantes procesos celulares como la apoptosis de la expresión alterada del miARN-15 durante el período de infección.Objetivo: Avaliar o perfil de expressão do miRNA-15 envolvido nos fatores de regulação da apoptose celular. Metodologia: Usamos a cepa asiática H818308 do ZIKV sem danos neurológicos. As inoculações ocorreram em células renais embrionárias humanas (HEK-293). Após a inoculação, as amostras foram extraídas para quantificação RT-qPCR do RNA viral e miR-15. O nível de ativação das caspases 1, 3/7 e 8 das células foi realizado por quimofluorescência. Resultados: A infecção pelo ZIKV altera a expressão de genes e seus reguladores, afetando diversos processos fisiológicos celulares, como a apoptose. Conclusão: Portanto, é importante enfatizar que as células progenitoras renais (HEK-293) são suscetíveis à infecção por VZIK. A desregulação genética resultante da infecção afeta diretamente processos celulares importantes, como a apoptose da expressão desordenada do miRNA-15 durante o período da infecção.Research, Society and Development2020-12-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1069910.33448/rsd-v9i12.10699Research, Society and Development; Vol. 9 No. 12; e3991210699Research, Society and Development; Vol. 9 Núm. 12; e3991210699Research, Society and Development; v. 9 n. 12; e39912106992525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/10699/9636Copyright (c) 2020 Jardel Fábio Lopes Ferreira; Murilo Tavares Amorim; Walter Félix Franco Neto; Francisco Canindé Ferreira de Luna; Karla Fabiane Lopes de Melo; Gleissy Adriane Lima Borges; Ana Paula Sousa Araújo; Gustavo Moraes Holanda; Samir Mansour Moraes Casseb; Ana Cecília Ribeiro Cruzhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFerreira, Jardel Fábio Lopes Amorim, Murilo Tavares Franco Neto, Walter Félix Luna, Francisco Canindé Ferreira de Melo, Karla Fabiane Lopes de Borges, Gleissy Adriane Lima Araújo, Ana Paula Sousa Holanda, Gustavo Moraes Casseb, Samir Mansour Moraes Cruz, Ana Cecília Ribeiro 2020-12-30T23:32:22Zoai:ojs.pkp.sfu.ca:article/10699Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:32:42.720399Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases
La infección por el Vírus Zika en células humanas altera el perfil de expresión del miRNA-15 y la activación de caspasas apoptóticas
Infecção por Zika Vírus em células humanas altera o perfil de expressão de miRNA-15 e ativação de caspases apoptóticas
title Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases
spellingShingle Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases
Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases
Ferreira, Jardel Fábio Lopes
Virus zika
Apoptose
mRNA-15
HEK-293.
Virus zika
Apoptosis
miRNA-15
HEK-293.
Zika virus
Apoptosis
miRNA-15
HEK-293.
Ferreira, Jardel Fábio Lopes
Virus zika
Apoptose
mRNA-15
HEK-293.
Virus zika
Apoptosis
miRNA-15
HEK-293.
Zika virus
Apoptosis
miRNA-15
HEK-293.
title_short Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases
title_full Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases
title_fullStr Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases
Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases
title_full_unstemmed Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases
Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases
title_sort Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases
author Ferreira, Jardel Fábio Lopes
author_facet Ferreira, Jardel Fábio Lopes
Ferreira, Jardel Fábio Lopes
Amorim, Murilo Tavares
Franco Neto, Walter Félix
Luna, Francisco Canindé Ferreira de
Melo, Karla Fabiane Lopes de
Borges, Gleissy Adriane Lima
Araújo, Ana Paula Sousa
Holanda, Gustavo Moraes
Casseb, Samir Mansour Moraes
Cruz, Ana Cecília Ribeiro
Amorim, Murilo Tavares
Franco Neto, Walter Félix
Luna, Francisco Canindé Ferreira de
Melo, Karla Fabiane Lopes de
Borges, Gleissy Adriane Lima
Araújo, Ana Paula Sousa
Holanda, Gustavo Moraes
Casseb, Samir Mansour Moraes
Cruz, Ana Cecília Ribeiro
author_role author
author2 Amorim, Murilo Tavares
Franco Neto, Walter Félix
Luna, Francisco Canindé Ferreira de
Melo, Karla Fabiane Lopes de
Borges, Gleissy Adriane Lima
Araújo, Ana Paula Sousa
Holanda, Gustavo Moraes
Casseb, Samir Mansour Moraes
Cruz, Ana Cecília Ribeiro
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ferreira, Jardel Fábio Lopes
Amorim, Murilo Tavares
Franco Neto, Walter Félix
Luna, Francisco Canindé Ferreira de
Melo, Karla Fabiane Lopes de
Borges, Gleissy Adriane Lima
Araújo, Ana Paula Sousa
Holanda, Gustavo Moraes
Casseb, Samir Mansour Moraes
Cruz, Ana Cecília Ribeiro
dc.subject.por.fl_str_mv Virus zika
Apoptose
mRNA-15
HEK-293.
Virus zika
Apoptosis
miRNA-15
HEK-293.
Zika virus
Apoptosis
miRNA-15
HEK-293.
topic Virus zika
Apoptose
mRNA-15
HEK-293.
Virus zika
Apoptosis
miRNA-15
HEK-293.
Zika virus
Apoptosis
miRNA-15
HEK-293.
description Objective: Evaluate the miRNA-15 expression profile involved in cellular apoptotic regulation factors. Methodology: We used the H818308 Asian strain of ZIKV without neurological damage. The inoculations occurred in human embryonic kidney cells (HEK-293). After inoculation, samples were extracted for RT-qPCR quantification of viral RNA and miR-15. The level of activation of caspases 1, 3/7 and 8 of cells was performed using chemofluorescence. Results: The ZIKV infection alters the expression of genes and their regulators, affecting several cellular physiological processes such as apoptosis. Conclusion: Therefore, it is important to emphasize that renal progenitor cells (HEK-293) are susceptible to VZIK infection. The genetic deregulation resulting from infection directly affects important cellular processes such as apoptosis from the disordered miRNA-15 expression during the infection period.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-13
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/10699
10.33448/rsd-v9i12.10699
url https://rsdjournal.org/index.php/rsd/article/view/10699
identifier_str_mv 10.33448/rsd-v9i12.10699
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/10699/9636
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 9 No. 12; e3991210699
Research, Society and Development; Vol. 9 Núm. 12; e3991210699
Research, Society and Development; v. 9 n. 12; e3991210699
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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dc.identifier.doi.none.fl_str_mv 10.33448/rsd-v9i12.10699