Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases

• Autor(a) principal: Ferreira, Jardel Fábio Lopes
• Data de Publicação: 2020
• Outros Autores: Amorim, Murilo Tavares, Franco Neto, Walter Félix, Luna, Francisco Canindé Ferreira de, Melo, Karla Fabiane Lopes de, Borges, Gleissy Adriane Lima, Araújo, Ana Paula Sousa, Holanda, Gustavo Moraes, Casseb, Samir Mansour Moraes, Cruz, Ana Cecília Ribeiro
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Research, Society and Development
• DOI: 10.33448/rsd-v9i12.10699
• Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/10699
• topic: Virus zika; Apoptose; mRNA-15; HEK-293.; Virus zika; Apoptosis; miRNA-15; HEK-293.; Zika virus; Apoptosis; miRNA-15; HEK-293.

Objective: Evaluate the miRNA-15 expression profile involved in cellular apoptotic regulation factors. Methodology: We used the H818308 Asian strain of ZIKV without neurological damage. The inoculations occurred in human embryonic kidney cells (HEK-293). After inoculation, samples were extracted for RT-qPCR quantification of viral RNA and miR-15. The level of activation of caspases 1, 3/7 and 8 of cells was performed using chemofluorescence. Results: The ZIKV infection alters the expression of genes and their regulators, affecting several cellular physiological processes such as apoptosis. Conclusion: Therefore, it is important to emphasize that renal progenitor cells (HEK-293) are susceptible to VZIK infection. The genetic deregulation resulting from infection directly affects important cellular processes such as apoptosis from the disordered miRNA-15 expression during the infection period.