Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
DOI: | 10.33448/rsd-v9i12.10699 |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/10699 |
Resumo: | Objective: Evaluate the miRNA-15 expression profile involved in cellular apoptotic regulation factors. Methodology: We used the H818308 Asian strain of ZIKV without neurological damage. The inoculations occurred in human embryonic kidney cells (HEK-293). After inoculation, samples were extracted for RT-qPCR quantification of viral RNA and miR-15. The level of activation of caspases 1, 3/7 and 8 of cells was performed using chemofluorescence. Results: The ZIKV infection alters the expression of genes and their regulators, affecting several cellular physiological processes such as apoptosis. Conclusion: Therefore, it is important to emphasize that renal progenitor cells (HEK-293) are susceptible to VZIK infection. The genetic deregulation resulting from infection directly affects important cellular processes such as apoptosis from the disordered miRNA-15 expression during the infection period. |
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oai:ojs.pkp.sfu.ca:article/10699 |
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UNIFEI |
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Research, Society and Development |
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Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspasesLa infección por el Vírus Zika en células humanas altera el perfil de expresión del miRNA-15 y la activación de caspasas apoptóticasInfecção por Zika Vírus em células humanas altera o perfil de expressão de miRNA-15 e ativação de caspases apoptóticasVirus zikaApoptosemRNA-15HEK-293.Virus zikaApoptosismiRNA-15HEK-293.Zika virusApoptosismiRNA-15HEK-293.Objective: Evaluate the miRNA-15 expression profile involved in cellular apoptotic regulation factors. Methodology: We used the H818308 Asian strain of ZIKV without neurological damage. The inoculations occurred in human embryonic kidney cells (HEK-293). After inoculation, samples were extracted for RT-qPCR quantification of viral RNA and miR-15. The level of activation of caspases 1, 3/7 and 8 of cells was performed using chemofluorescence. Results: The ZIKV infection alters the expression of genes and their regulators, affecting several cellular physiological processes such as apoptosis. Conclusion: Therefore, it is important to emphasize that renal progenitor cells (HEK-293) are susceptible to VZIK infection. The genetic deregulation resulting from infection directly affects important cellular processes such as apoptosis from the disordered miRNA-15 expression during the infection period.Objetivo: Evaluar el perfil de expresión de miARN-15 implicado en factores de regulación apoptótica celular. Metodología: Utilizamos la cepa asiática H818308 de ZIKV sin daño neurológico. Las inoculaciones se produjeron en células renales embrionarias humanas (HEK-293). Después de la inoculación, se extrajeron muestras para la cuantificación RT-qPCR de ARN viral y miR-15. El nivel de activación de las caspasas 1, 3/7 y 8 de las células se realizó mediante quimiofluorescencia. Resultados: La infección por ZIKV altera la expresión de genes y sus reguladores, afectando varios procesos fisiológicos celulares como la apoptosis. Conclusión: Por lo tanto, es importante enfatizar que las células progenitoras renales (HEK-293) son susceptibles a la infección por VZIK. La desregulación genética resultante de la infección afecta directamente a importantes procesos celulares como la apoptosis de la expresión alterada del miARN-15 durante el período de infección.Objetivo: Avaliar o perfil de expressão do miRNA-15 envolvido nos fatores de regulação da apoptose celular. Metodologia: Usamos a cepa asiática H818308 do ZIKV sem danos neurológicos. As inoculações ocorreram em células renais embrionárias humanas (HEK-293). Após a inoculação, as amostras foram extraídas para quantificação RT-qPCR do RNA viral e miR-15. O nível de ativação das caspases 1, 3/7 e 8 das células foi realizado por quimofluorescência. Resultados: A infecção pelo ZIKV altera a expressão de genes e seus reguladores, afetando diversos processos fisiológicos celulares, como a apoptose. Conclusão: Portanto, é importante enfatizar que as células progenitoras renais (HEK-293) são suscetíveis à infecção por VZIK. A desregulação genética resultante da infecção afeta diretamente processos celulares importantes, como a apoptose da expressão desordenada do miRNA-15 durante o período da infecção.Research, Society and Development2020-12-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1069910.33448/rsd-v9i12.10699Research, Society and Development; Vol. 9 No. 12; e3991210699Research, Society and Development; Vol. 9 Núm. 12; e3991210699Research, Society and Development; v. 9 n. 12; e39912106992525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/10699/9636Copyright (c) 2020 Jardel Fábio Lopes Ferreira; Murilo Tavares Amorim; Walter Félix Franco Neto; Francisco Canindé Ferreira de Luna; Karla Fabiane Lopes de Melo; Gleissy Adriane Lima Borges; Ana Paula Sousa Araújo; Gustavo Moraes Holanda; Samir Mansour Moraes Casseb; Ana Cecília Ribeiro Cruzhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFerreira, Jardel Fábio Lopes Amorim, Murilo Tavares Franco Neto, Walter Félix Luna, Francisco Canindé Ferreira de Melo, Karla Fabiane Lopes de Borges, Gleissy Adriane Lima Araújo, Ana Paula Sousa Holanda, Gustavo Moraes Casseb, Samir Mansour Moraes Cruz, Ana Cecília Ribeiro 2020-12-30T23:32:22Zoai:ojs.pkp.sfu.ca:article/10699Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:32:42.720399Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases La infección por el Vírus Zika en células humanas altera el perfil de expresión del miRNA-15 y la activación de caspasas apoptóticas Infecção por Zika Vírus em células humanas altera o perfil de expressão de miRNA-15 e ativação de caspases apoptóticas |
title |
Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases |
spellingShingle |
Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases Ferreira, Jardel Fábio Lopes Virus zika Apoptose mRNA-15 HEK-293. Virus zika Apoptosis miRNA-15 HEK-293. Zika virus Apoptosis miRNA-15 HEK-293. Ferreira, Jardel Fábio Lopes Virus zika Apoptose mRNA-15 HEK-293. Virus zika Apoptosis miRNA-15 HEK-293. Zika virus Apoptosis miRNA-15 HEK-293. |
title_short |
Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases |
title_full |
Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases |
title_fullStr |
Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases |
title_full_unstemmed |
Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases |
title_sort |
Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases |
author |
Ferreira, Jardel Fábio Lopes |
author_facet |
Ferreira, Jardel Fábio Lopes Ferreira, Jardel Fábio Lopes Amorim, Murilo Tavares Franco Neto, Walter Félix Luna, Francisco Canindé Ferreira de Melo, Karla Fabiane Lopes de Borges, Gleissy Adriane Lima Araújo, Ana Paula Sousa Holanda, Gustavo Moraes Casseb, Samir Mansour Moraes Cruz, Ana Cecília Ribeiro Amorim, Murilo Tavares Franco Neto, Walter Félix Luna, Francisco Canindé Ferreira de Melo, Karla Fabiane Lopes de Borges, Gleissy Adriane Lima Araújo, Ana Paula Sousa Holanda, Gustavo Moraes Casseb, Samir Mansour Moraes Cruz, Ana Cecília Ribeiro |
author_role |
author |
author2 |
Amorim, Murilo Tavares Franco Neto, Walter Félix Luna, Francisco Canindé Ferreira de Melo, Karla Fabiane Lopes de Borges, Gleissy Adriane Lima Araújo, Ana Paula Sousa Holanda, Gustavo Moraes Casseb, Samir Mansour Moraes Cruz, Ana Cecília Ribeiro |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira, Jardel Fábio Lopes Amorim, Murilo Tavares Franco Neto, Walter Félix Luna, Francisco Canindé Ferreira de Melo, Karla Fabiane Lopes de Borges, Gleissy Adriane Lima Araújo, Ana Paula Sousa Holanda, Gustavo Moraes Casseb, Samir Mansour Moraes Cruz, Ana Cecília Ribeiro |
dc.subject.por.fl_str_mv |
Virus zika Apoptose mRNA-15 HEK-293. Virus zika Apoptosis miRNA-15 HEK-293. Zika virus Apoptosis miRNA-15 HEK-293. |
topic |
Virus zika Apoptose mRNA-15 HEK-293. Virus zika Apoptosis miRNA-15 HEK-293. Zika virus Apoptosis miRNA-15 HEK-293. |
description |
Objective: Evaluate the miRNA-15 expression profile involved in cellular apoptotic regulation factors. Methodology: We used the H818308 Asian strain of ZIKV without neurological damage. The inoculations occurred in human embryonic kidney cells (HEK-293). After inoculation, samples were extracted for RT-qPCR quantification of viral RNA and miR-15. The level of activation of caspases 1, 3/7 and 8 of cells was performed using chemofluorescence. Results: The ZIKV infection alters the expression of genes and their regulators, affecting several cellular physiological processes such as apoptosis. Conclusion: Therefore, it is important to emphasize that renal progenitor cells (HEK-293) are susceptible to VZIK infection. The genetic deregulation resulting from infection directly affects important cellular processes such as apoptosis from the disordered miRNA-15 expression during the infection period. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/10699 10.33448/rsd-v9i12.10699 |
url |
https://rsdjournal.org/index.php/rsd/article/view/10699 |
identifier_str_mv |
10.33448/rsd-v9i12.10699 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/10699/9636 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 9 No. 12; e3991210699 Research, Society and Development; Vol. 9 Núm. 12; e3991210699 Research, Society and Development; v. 9 n. 12; e3991210699 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1822178887124123648 |
dc.identifier.doi.none.fl_str_mv |
10.33448/rsd-v9i12.10699 |