Genistein acts in bone metabolism and improves peri-implant healing in rats with estrogen deficiency
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/15061 |
Resumo: | The present study aimed to evaluate the peri-implantar bone healing in the presence of genistein treatment in ovariectomized rats. Thirty female rats with 4 months old were divided into 3 groups according to the experimental condition and the drug treatment: SHAM (rats submitted to the fictional surgery and gavage with 0.9% saline solution); OVX (rats submitted to bilateral ovariectomy and gavage with 0.9% saline solution); OVX GEN (rats submitted to bilateral ovariectomy and gavage with 1mg/day of genistein). 60 implants were installed, with two implants in each animal. The calcified group was subjected microcomputerized tomography and the parameters analysed was bone volume per tissue volume (BV/TV) and connective density (Cnn.Dn). The decalcified samples were evaluated through immunolabeling analysis, in order to detect the presence of RUNX2, Alkaline Phosphatase, Osteocalcin, Osteopontin and TRAP. All the quantitative data were submitted to the normality curve to determine the most adequate test. The significance level of p<0.05 was considered for all tests. The morphometric analysis of the OVX GEN group showed higher percentage of bone volume and lower connective density when compared with OVX. Immunohistochemical analysis favors expression. For the markers that positively label osteoblastic activity. This study shows that genistein therapy improves peri-implant bone healing in ovariectomized rats. |
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Genistein acts in bone metabolism and improves peri-implant healing in rats with estrogen deficiencyLa genisteína actúa sobre el metabolismo óseo y mejora la reparación periimplantaria en ratas con deficiencia de estrógenosGenisteína atua no metabolismo ósseo e melhora o reparo peri-implantar em ratas com deficiência de estrógenoOssoOsteoporoseGenisteínaImplantes dentários.HuesoOsteoporosisGenisteínaImplantes dentales.BoneOsteoporosisGenisteinDental implants.The present study aimed to evaluate the peri-implantar bone healing in the presence of genistein treatment in ovariectomized rats. Thirty female rats with 4 months old were divided into 3 groups according to the experimental condition and the drug treatment: SHAM (rats submitted to the fictional surgery and gavage with 0.9% saline solution); OVX (rats submitted to bilateral ovariectomy and gavage with 0.9% saline solution); OVX GEN (rats submitted to bilateral ovariectomy and gavage with 1mg/day of genistein). 60 implants were installed, with two implants in each animal. The calcified group was subjected microcomputerized tomography and the parameters analysed was bone volume per tissue volume (BV/TV) and connective density (Cnn.Dn). The decalcified samples were evaluated through immunolabeling analysis, in order to detect the presence of RUNX2, Alkaline Phosphatase, Osteocalcin, Osteopontin and TRAP. All the quantitative data were submitted to the normality curve to determine the most adequate test. The significance level of p<0.05 was considered for all tests. The morphometric analysis of the OVX GEN group showed higher percentage of bone volume and lower connective density when compared with OVX. Immunohistochemical analysis favors expression. For the markers that positively label osteoblastic activity. This study shows that genistein therapy improves peri-implant bone healing in ovariectomized rats.El presente estudio tuvo como objetivo evaluar la cicatrización ósea periimplantar en presencia de tratamiento con genisteína en ratas ovariectomizadas. Treinta ratas hembras de 4 meses de edad se dividieron en 3 grupos según la condición experimental y el tratamiento farmacológico: SHAM (ratas sometidas a la cirugía ficticia y alimentación por sonda con solución salina al 0,9%); OVX (ratas sometidas a ovariectomía bilateral y alimentación por sonda con solución salina al 0,9%); OVX GEN (ratas sometidas a ovariectomía bilateral y sonda con 1 mg / día de genisteína). Se instalaron 60 implantes, con dos implantes en cada animal. El grupo calcificado fue sometido a tomografía microcomputerizada y los parámetros analizados fueron volumen óseo por volumen tisular (BV / TV) y densidad conectiva (Cnn.Dn). Las muestras descalcificadas se evaluaron mediante análisis de inmunomarcaje, con el fin de detectar la presencia de RUNX2, Fosfatasa Alcalina, Osteocalcina, Osteopontina y TRAP. Todos los datos cuantitativos se sometieron a la curva de normalidad para determinar la prueba más adecuada. Se consideró el nivel de significancia de p <0.05 para todas las pruebas. El análisis morfométrico del grupo OVX GEN mostró un mayor porcentaje de volumen óseo y una menor densidad conectiva en comparación con OVX. El análisis inmunohistoquímico favorece la expresión de los marcadores que actúan positivamente sobre la actividad osteoblástica. Este estudio muestra que la terapia con genisteína mejora la curación ósea periimplantaria en ratas ovariectomizadas.O presente estudo teve como objetivo avaliar a consolidação óssea peri-implantar na presença de tratamento com genisteína em ratas ovariectomizadas. Trinta ratas com 4 meses de idade foram divididas em 3 grupos de acordo com a condição experimental e o tratamento medicamentoso: SHAM (ratas submetidas à cirurgia fictícia e gavagem com solução fisiológica 0,9%); OVX (ratas submetidas a ovariectomia bilateral e gavagem com solução salina 0,9%); OVX GEN (ratas submetidas a ovariectomia bilateral e gavagem com 1mg / dia de genisteína). Foram instalados 60 implantes, com dois implantes em cada animal. O grupo calcificado foi submetido a tomografia microcomputerizada e os parâmetros analisados foram volume ósseo por volume de tecido (VB / VT) e densidade conectiva (Cnn.Dn). As amostras descalcificadas foram avaliadas por meio de análise de imunomarcação, a fim de detectar a presença de RUNX2, Fosfatase Alcalina, Osteocalcina, Osteopontina e TRAP. Todos os dados quantitativos foram submetidos à curva de normalidade para determinar o teste mais adequado. O nível de significância de p <0,05 foi considerado para todos os testes. A análise morfométrica do grupo OVX GEN mostrou maior porcentagem de volume ósseo e menor densidade conectiva quando comparada com OVX. A análise imunohistoquímica favorece a expressão dos marcadores que atuam positivamente na atividade osteoblástica. Este estudo mostra que a terapia com genisteína melhora a cicatrização óssea peri-implantar em ratas ovariectomizadas.Research, Society and Development2021-05-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1506110.33448/rsd-v10i5.15061Research, Society and Development; Vol. 10 No. 5; e36410515061Research, Society and Development; Vol. 10 Núm. 5; e36410515061Research, Society and Development; v. 10 n. 5; e364105150612525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/15061/13487Copyright (c) 2021 Naara Gabriela Monteiro; Fábio Roberto De Souza Batista ; Maria Isabela Lopes Gandolfo ; Leonardo Perez Faverani; Letícia Pitol Palin ; Gabriel Mulinari-Santos; Pedro Henrique Silva Gomes Ferreira; Francisley Ávila Souza; Roberta Okamotohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMonteiro, Naara GabrielaDe Souza Batista , Fábio Roberto Gandolfo , Maria Isabela Lopes Faverani, Leonardo Perez Pitol Palin , Letícia Mulinari-Santos, GabrielGomes Ferreira, Pedro Henrique SilvaSouza, Francisley Ávila Okamoto, Roberta 2021-05-17T18:20:49Zoai:ojs.pkp.sfu.ca:article/15061Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:35:58.867196Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Genistein acts in bone metabolism and improves peri-implant healing in rats with estrogen deficiency La genisteína actúa sobre el metabolismo óseo y mejora la reparación periimplantaria en ratas con deficiencia de estrógenos Genisteína atua no metabolismo ósseo e melhora o reparo peri-implantar em ratas com deficiência de estrógeno |
| title |
Genistein acts in bone metabolism and improves peri-implant healing in rats with estrogen deficiency |
| spellingShingle |
Genistein acts in bone metabolism and improves peri-implant healing in rats with estrogen deficiency Monteiro, Naara Gabriela Osso Osteoporose Genisteína Implantes dentários. Hueso Osteoporosis Genisteína Implantes dentales. Bone Osteoporosis Genistein Dental implants. |
| title_short |
Genistein acts in bone metabolism and improves peri-implant healing in rats with estrogen deficiency |
| title_full |
Genistein acts in bone metabolism and improves peri-implant healing in rats with estrogen deficiency |
| title_fullStr |
Genistein acts in bone metabolism and improves peri-implant healing in rats with estrogen deficiency |
| title_full_unstemmed |
Genistein acts in bone metabolism and improves peri-implant healing in rats with estrogen deficiency |
| title_sort |
Genistein acts in bone metabolism and improves peri-implant healing in rats with estrogen deficiency |
| author |
Monteiro, Naara Gabriela |
| author_facet |
Monteiro, Naara Gabriela De Souza Batista , Fábio Roberto Gandolfo , Maria Isabela Lopes Faverani, Leonardo Perez Pitol Palin , Letícia Mulinari-Santos, Gabriel Gomes Ferreira, Pedro Henrique Silva Souza, Francisley Ávila Okamoto, Roberta |
| author_role |
author |
| author2 |
De Souza Batista , Fábio Roberto Gandolfo , Maria Isabela Lopes Faverani, Leonardo Perez Pitol Palin , Letícia Mulinari-Santos, Gabriel Gomes Ferreira, Pedro Henrique Silva Souza, Francisley Ávila Okamoto, Roberta |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Monteiro, Naara Gabriela De Souza Batista , Fábio Roberto Gandolfo , Maria Isabela Lopes Faverani, Leonardo Perez Pitol Palin , Letícia Mulinari-Santos, Gabriel Gomes Ferreira, Pedro Henrique Silva Souza, Francisley Ávila Okamoto, Roberta |
| dc.subject.por.fl_str_mv |
Osso Osteoporose Genisteína Implantes dentários. Hueso Osteoporosis Genisteína Implantes dentales. Bone Osteoporosis Genistein Dental implants. |
| topic |
Osso Osteoporose Genisteína Implantes dentários. Hueso Osteoporosis Genisteína Implantes dentales. Bone Osteoporosis Genistein Dental implants. |
| description |
The present study aimed to evaluate the peri-implantar bone healing in the presence of genistein treatment in ovariectomized rats. Thirty female rats with 4 months old were divided into 3 groups according to the experimental condition and the drug treatment: SHAM (rats submitted to the fictional surgery and gavage with 0.9% saline solution); OVX (rats submitted to bilateral ovariectomy and gavage with 0.9% saline solution); OVX GEN (rats submitted to bilateral ovariectomy and gavage with 1mg/day of genistein). 60 implants were installed, with two implants in each animal. The calcified group was subjected microcomputerized tomography and the parameters analysed was bone volume per tissue volume (BV/TV) and connective density (Cnn.Dn). The decalcified samples were evaluated through immunolabeling analysis, in order to detect the presence of RUNX2, Alkaline Phosphatase, Osteocalcin, Osteopontin and TRAP. All the quantitative data were submitted to the normality curve to determine the most adequate test. The significance level of p<0.05 was considered for all tests. The morphometric analysis of the OVX GEN group showed higher percentage of bone volume and lower connective density when compared with OVX. Immunohistochemical analysis favors expression. For the markers that positively label osteoblastic activity. This study shows that genistein therapy improves peri-implant bone healing in ovariectomized rats. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-05-09 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/15061 10.33448/rsd-v10i5.15061 |
| url |
https://rsdjournal.org/index.php/rsd/article/view/15061 |
| identifier_str_mv |
10.33448/rsd-v10i5.15061 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/15061/13487 |
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https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development; Vol. 10 No. 5; e36410515061 Research, Society and Development; Vol. 10 Núm. 5; e36410515061 Research, Society and Development; v. 10 n. 5; e36410515061 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Universidade Federal de Itajubá (UNIFEI) |
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UNIFEI |
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UNIFEI |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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rsd.articles@gmail.com |
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1797052748785516544 |