Antiproliferative potential of the essential oil of Origanum majorana Linn. in melanoma cells and intracellular effects on neoplastic and non-neoplastic cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/17788 |
Resumo: | The objectives of this study were to evaluate the intracellular activity of the essential oil of Origanum majorana (MARJ) in Madin Darby Bovine Kidney (MDBK) and murine metastatic melanoma (B16F10) and elucidate your chemical composition. Through flow cytometry parameters were analyzed membrane fluidity (F), reactive oxygen species (ROS), DNA fragmentation index (IFD), mitochondrial membrane potential (MMP), cell cycle, plasma membrane lipoperoxidation (LPO), cell viability, necrosis and apoptosis of cell lines treated with MARJ at concentrations of 0.25, 0.5 and 1 mg/mL for 24 hours. The composition of MARJ was determined by gas chromatography, and its major components were terpenes: hydrated cis-sabinene, 4-terpineol and gamma-terpinene. The results showed that all MARJ concentrations reduced ROS, LPO and S phase cells of the MDBK lineage. In B16F10 cells, MARJ treatments reduced ROS and cells in G2 phase, increasing cells in S phase. Concentrations of 0.5 and 1 mg/mL caused a reduction in F and 0.5 mg/mL increased the MMP in the MDBK lineage, while LPO was reduced at the concentration of 0.5 mg/mL in the B16F10 cells. The results of the evaluations of ROS production, plasma membrane integrity and LPO indicated antioxidant activity in the cells studied. Through cell cycle analysis, it was possible to observe that MARJ exerted an antiproliferative effect on melanoma cells, being able to interrupt the multiplication of this cell type. |
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Antiproliferative potential of the essential oil of Origanum majorana Linn. in melanoma cells and intracellular effects on neoplastic and non-neoplastic cellsPotencial antiproliferativo del aceite esencial de Origanum majorana Linn. en células de melanoma y sus efectos intracelulares en células neoplásicas y no neoplásicasPotencial antiproliferativo do óleo essencial de Origanum majorana Linn. em células de melanoma e seus efeitos intracelulares em células neoplásicas e não-neoplásicasFlow cytometryCancerCytotoxicityMelanomaMarjoram.Citometría de flujoCáncerCitotoxicidadMelanomaMejorana.Citometria de fluxoCâncerCitotoxicidadeMelanomaManjerona.The objectives of this study were to evaluate the intracellular activity of the essential oil of Origanum majorana (MARJ) in Madin Darby Bovine Kidney (MDBK) and murine metastatic melanoma (B16F10) and elucidate your chemical composition. Through flow cytometry parameters were analyzed membrane fluidity (F), reactive oxygen species (ROS), DNA fragmentation index (IFD), mitochondrial membrane potential (MMP), cell cycle, plasma membrane lipoperoxidation (LPO), cell viability, necrosis and apoptosis of cell lines treated with MARJ at concentrations of 0.25, 0.5 and 1 mg/mL for 24 hours. The composition of MARJ was determined by gas chromatography, and its major components were terpenes: hydrated cis-sabinene, 4-terpineol and gamma-terpinene. The results showed that all MARJ concentrations reduced ROS, LPO and S phase cells of the MDBK lineage. In B16F10 cells, MARJ treatments reduced ROS and cells in G2 phase, increasing cells in S phase. Concentrations of 0.5 and 1 mg/mL caused a reduction in F and 0.5 mg/mL increased the MMP in the MDBK lineage, while LPO was reduced at the concentration of 0.5 mg/mL in the B16F10 cells. The results of the evaluations of ROS production, plasma membrane integrity and LPO indicated antioxidant activity in the cells studied. Through cell cycle analysis, it was possible to observe that MARJ exerted an antiproliferative effect on melanoma cells, being able to interrupt the multiplication of this cell type.Los objetivos de este estudio fueron evaluar la actividad intracelular del aceite esencial de Origanum majorana (MARJ) en linajes de Madin Darby Bovine Kidney (MDBK) y melanoma metastásico murino (B16F10) y dilucidar su composición química. Mediante citometría de flujo se analizaron parámetros de fluidez de membrana (F), especies reactivas de oxígeno (ERO), índice de fragmentación del ADN (IFA), potencial de membrana mitocondrial (PMM), ciclo celular, lipoperoxidación de la membrana plasmática (LPO), viabilidad celular, necrosis y apoptosis de linajes celulares tratadas con MARJ a concentraciones de 0,25, 0,5 y 1 mg/mL durante 24 horas. La composición de MARJ se determinó por cromatografía de gases, y sus componentes principales fueron terpenos: cis-sabineno hidratado, 4-terpineol y gamma-terpineno. Los resultados mostraron que todas las concentraciones de MARJ redujeron ERO, LPO y células de fase S del linaje MDBK. En las células B16F10, los tratamientos MARJ redujeron ERO y células en fase G2, aumentando las células en fase S. Concentraciones de 0.5 y 1 mg/mL provocaron una reducción en F y 0.5 mg/mL aumentaron las PMM en el linaje MDBK, mientras que la LPO se redujo a la concentración de 0,5 mg/mL en las células B16F10. Los resultados de las evaluaciones de producción de ERO, integridad de la membrana plasmática y LPO indicaron actividad antioxidante en las células estudiadas. A través del análisis del ciclo celular, se pudo observar que MARJ ejercía un efecto antiproliferativo sobre las células del melanoma, pudiendo interrumpir la multiplicación de este tipo celular.Os objetivos desse estudo foram avaliar a atividade intracelular do óleo essencial de Origanum majorana (MARJ) nas linhagens Madin Darby Bovine Kidney (MDBK) e de melanoma metastático murino (B16F10) e elucidar a sua composição química. Através da citometria de fluxo foram analisados os parâmetros fluidez de membrana (F), espécies reativas de oxigênio (ERO), índice de fragmentação de DNA (IFD), potencial de membrana mitocondrial (PMM), ciclo celular, lipoperoxidação da membrana plasmática (LPO), viabilidade celular, necrose e apoptose das linhagens celulares tratadas com MARJ nas concentrações de 0,25, 0,5 e 1 mg/mL por 24 horas. A composição do MARJ foi determinada através de cromatografia gasosa, sendo seus componentes majoritários os terpenos: cis-sabineno hidratado, 4-terpineol e gama-terpineno. Os resultados mostraram que todas as concentrações de MARJ reduziram ERO, LPO e as células em fase S da linhagem MDBK. Nas células B16F10, os tratamentos com MARJ reduziram ERO e as células em fase G2, aumentando as células na fase S. As concentrações de 0,5 e 1 mg/mL causaram redução na F e 0,5 mg/mL aumentou o PMM na linhagem MDBK, enquanto LPO foi reduzida na concentração de 0,5 mg/mL nas células B16F10. Os resultados das avaliações da produção de ERO, da integridade da membrana plasmática e de LPO indicaram atividade antioxidante nas linhagens estudadas. Através da análise do ciclo celular foi possível observar que MARJ exerceu efeito antiproliferativo sobre as células de melanoma, sendo capaz de interromper a multiplicação desse tipo celular.Research, Society and Development2021-07-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1778810.33448/rsd-v10i9.17788Research, Society and Development; Vol. 10 No. 9; e7110917788Research, Society and Development; Vol. 10 Núm. 9; e7110917788Research, Society and Development; v. 10 n. 9; e71109177882525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/17788/15906Copyright (c) 2021 Cristine Cioato da Silva; Claudia Giordani; Tony Picoli; Karina Affeldt Guterres; Soliane Carra Perera; Gabriela Almeida Capella; Stefanie Bressan Waller; Carine Dahl Corcini; Antonio Sergio Varela Junior; Rogério Antonio Freitag; Geferson Fischer; Marlete Brum Cleffhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva, Cristine Cioato da Giordani, Claudia Picoli, TonyGuterres, Karina Affeldt Perera, Soliane Carra Capella, Gabriela Almeida Waller, Stefanie Bressan Corcini, Carine Dahl Varela Junior, Antonio SergioFreitag, Rogério AntonioFischer, GefersonCleff, Marlete Brum 2021-09-12T14:28:06Zoai:ojs.pkp.sfu.ca:article/17788Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:38:04.095544Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Antiproliferative potential of the essential oil of Origanum majorana Linn. in melanoma cells and intracellular effects on neoplastic and non-neoplastic cells Potencial antiproliferativo del aceite esencial de Origanum majorana Linn. en células de melanoma y sus efectos intracelulares en células neoplásicas y no neoplásicas Potencial antiproliferativo do óleo essencial de Origanum majorana Linn. em células de melanoma e seus efeitos intracelulares em células neoplásicas e não-neoplásicas |
| title |
Antiproliferative potential of the essential oil of Origanum majorana Linn. in melanoma cells and intracellular effects on neoplastic and non-neoplastic cells |
| spellingShingle |
Antiproliferative potential of the essential oil of Origanum majorana Linn. in melanoma cells and intracellular effects on neoplastic and non-neoplastic cells Silva, Cristine Cioato da Flow cytometry Cancer Cytotoxicity Melanoma Marjoram. Citometría de flujo Cáncer Citotoxicidad Melanoma Mejorana. Citometria de fluxo Câncer Citotoxicidade Melanoma Manjerona. |
| title_short |
Antiproliferative potential of the essential oil of Origanum majorana Linn. in melanoma cells and intracellular effects on neoplastic and non-neoplastic cells |
| title_full |
Antiproliferative potential of the essential oil of Origanum majorana Linn. in melanoma cells and intracellular effects on neoplastic and non-neoplastic cells |
| title_fullStr |
Antiproliferative potential of the essential oil of Origanum majorana Linn. in melanoma cells and intracellular effects on neoplastic and non-neoplastic cells |
| title_full_unstemmed |
Antiproliferative potential of the essential oil of Origanum majorana Linn. in melanoma cells and intracellular effects on neoplastic and non-neoplastic cells |
| title_sort |
Antiproliferative potential of the essential oil of Origanum majorana Linn. in melanoma cells and intracellular effects on neoplastic and non-neoplastic cells |
| author |
Silva, Cristine Cioato da |
| author_facet |
Silva, Cristine Cioato da Giordani, Claudia Picoli, Tony Guterres, Karina Affeldt Perera, Soliane Carra Capella, Gabriela Almeida Waller, Stefanie Bressan Corcini, Carine Dahl Varela Junior, Antonio Sergio Freitag, Rogério Antonio Fischer, Geferson Cleff, Marlete Brum |
| author_role |
author |
| author2 |
Giordani, Claudia Picoli, Tony Guterres, Karina Affeldt Perera, Soliane Carra Capella, Gabriela Almeida Waller, Stefanie Bressan Corcini, Carine Dahl Varela Junior, Antonio Sergio Freitag, Rogério Antonio Fischer, Geferson Cleff, Marlete Brum |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Cristine Cioato da Giordani, Claudia Picoli, Tony Guterres, Karina Affeldt Perera, Soliane Carra Capella, Gabriela Almeida Waller, Stefanie Bressan Corcini, Carine Dahl Varela Junior, Antonio Sergio Freitag, Rogério Antonio Fischer, Geferson Cleff, Marlete Brum |
| dc.subject.por.fl_str_mv |
Flow cytometry Cancer Cytotoxicity Melanoma Marjoram. Citometría de flujo Cáncer Citotoxicidad Melanoma Mejorana. Citometria de fluxo Câncer Citotoxicidade Melanoma Manjerona. |
| topic |
Flow cytometry Cancer Cytotoxicity Melanoma Marjoram. Citometría de flujo Cáncer Citotoxicidad Melanoma Mejorana. Citometria de fluxo Câncer Citotoxicidade Melanoma Manjerona. |
| description |
The objectives of this study were to evaluate the intracellular activity of the essential oil of Origanum majorana (MARJ) in Madin Darby Bovine Kidney (MDBK) and murine metastatic melanoma (B16F10) and elucidate your chemical composition. Through flow cytometry parameters were analyzed membrane fluidity (F), reactive oxygen species (ROS), DNA fragmentation index (IFD), mitochondrial membrane potential (MMP), cell cycle, plasma membrane lipoperoxidation (LPO), cell viability, necrosis and apoptosis of cell lines treated with MARJ at concentrations of 0.25, 0.5 and 1 mg/mL for 24 hours. The composition of MARJ was determined by gas chromatography, and its major components were terpenes: hydrated cis-sabinene, 4-terpineol and gamma-terpinene. The results showed that all MARJ concentrations reduced ROS, LPO and S phase cells of the MDBK lineage. In B16F10 cells, MARJ treatments reduced ROS and cells in G2 phase, increasing cells in S phase. Concentrations of 0.5 and 1 mg/mL caused a reduction in F and 0.5 mg/mL increased the MMP in the MDBK lineage, while LPO was reduced at the concentration of 0.5 mg/mL in the B16F10 cells. The results of the evaluations of ROS production, plasma membrane integrity and LPO indicated antioxidant activity in the cells studied. Through cell cycle analysis, it was possible to observe that MARJ exerted an antiproliferative effect on melanoma cells, being able to interrupt the multiplication of this cell type. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-07-21 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://rsdjournal.org/index.php/rsd/article/view/17788 10.33448/rsd-v10i9.17788 |
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https://rsdjournal.org/index.php/rsd/article/view/17788 |
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10.33448/rsd-v10i9.17788 |
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por |
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por |
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https://rsdjournal.org/index.php/rsd/article/view/17788/15906 |
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https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development; Vol. 10 No. 9; e7110917788 Research, Society and Development; Vol. 10 Núm. 9; e7110917788 Research, Society and Development; v. 10 n. 9; e7110917788 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Universidade Federal de Itajubá (UNIFEI) |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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