Microbiological, serological and molecular diagnosis of abscess of withers in equine in the State of Pará
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/17754 |
Resumo: | There are several infectious diseases that affect equines and can cause large economic losses in this sector and damage to animal welfare. In this species, abscessing lesions of a purulent character preferentially in the withers are strongly suggestive of brucellosis. However, few studies have been carried out on the disease in this species, making its identification and control difficult due to the lack of standardization in the interpretation of results. Therefore, samples of whole blood, serum and purulent secretion were collected from three horses. The animals came from the state of Pará. The diagnostic methods selected were the Rose-Bengal plate test (RBPT) and Polymerase Chain Reaction (PCR), for the negative molecular results for brucellosis, microbiological analysis were chosen. Although part of the samples were positive for brucellosis in serological tests, all of them showed negative results for PCR. In bacterial cultivation, species were isolated Streptococcus equi subsp. zooepidemicus, Enterococcus faecalis e Staphylococcus sciuri subsp. lentus., which showed resistance to antibacterials, especially the E. faecalis isolate classified as MDR (multi drug resistant). In conclusion, the best form of diagnosis is the joint evaluation of several tests, both screening and confirmatory, in order to obtain a definitive diagnosis with assertiveness. |
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Microbiological, serological and molecular diagnosis of abscess of withers in equine in the State of ParáDiagnóstico microbiológico, serológico y molecular de los abscesos en cruz de caballo en el Estado de ParáDiagnóstico microbiológico, sorológico e molecular de abscessos de cernelha em equinos no Estado do ParáBruceloseBrucella melitensisEquinoDoença zoonótica.BrucelosisBrucella melitensisEquinoEnfermedad zoonótica.BrucellosisBrucella melitensisEquineZoonotic disease.There are several infectious diseases that affect equines and can cause large economic losses in this sector and damage to animal welfare. In this species, abscessing lesions of a purulent character preferentially in the withers are strongly suggestive of brucellosis. However, few studies have been carried out on the disease in this species, making its identification and control difficult due to the lack of standardization in the interpretation of results. Therefore, samples of whole blood, serum and purulent secretion were collected from three horses. The animals came from the state of Pará. The diagnostic methods selected were the Rose-Bengal plate test (RBPT) and Polymerase Chain Reaction (PCR), for the negative molecular results for brucellosis, microbiological analysis were chosen. Although part of the samples were positive for brucellosis in serological tests, all of them showed negative results for PCR. In bacterial cultivation, species were isolated Streptococcus equi subsp. zooepidemicus, Enterococcus faecalis e Staphylococcus sciuri subsp. lentus., which showed resistance to antibacterials, especially the E. faecalis isolate classified as MDR (multi drug resistant). In conclusion, the best form of diagnosis is the joint evaluation of several tests, both screening and confirmatory, in order to obtain a definitive diagnosis with assertiveness.Existen varias enfermedades infecciosas que afectan a los equinos y pueden causar grandes pérdidas económicas en este sector y daños al bienestar animal. En esta especie, las lesiones con abscesos de carácter purulento, preferentemente en la cruz, son muy sugestivas de brucelosis. Sin embargo, se han realizado pocos estudios sobre la enfermedad en esta especie, lo que dificulta su identificación y control por la falta de estandarización en la interpretación de los resultados. Por lo tanto, se recolectaron muestras de sangre completa, suero y secreción purulenta de tres caballos. Los animales procedían del estado de Pará. Los métodos de diagnóstico seleccionados fueron la prueba de placa del Rosa Bengala y la Reacción en Cadena de la Polimerasa (PCR), para los resultados moleculares negativos para brucelosis se eligió el análisis microbiológico. Aunque parte de las muestras resultaron positivas para brucelosis en las pruebas serológicas, todas arrojaron resultados negativos para la PCR. En cultivo bacteriano, se aislaron especies Streptococcus equi subsp. zooepidemicus, Enterococcus faecalis y Staphylococcus sciuri subsp. lentus., que mostró resistencia a los antibacterianos, especialmente al aislado de E. faecalis clasificado como multirresistente. En conclusión, la mejor forma de diagnóstico es la evaluación conjunta de varias pruebas, tanto de cribado como confirmatorias, para obtener un diagnóstico definitivo con asertividad.Existem diversas enfermidades infectocontagiosas que acometem os equinos podendo causar grandes perdas econômicas nesse setor e danos ao bem estar dos animais. Nessa espécie, lesões abscedantes de caráter purulento preferencialmente na região da cernelha são fortemente sugestivas de brucelose. Entretanto são poucos os estudos realizados sobre a doença nessa espécie, dificultando sua identificação e controle devido à falta de padronização na interpretação dos resultados. Desse modo, foram coletados a partir de tres equinos, amostras de sangue total, soro e secreção purulenta. Os animais eram provinientes do estado do Pará. Os métodos de diagnóstico selecionados foram os testes do Antígeno Acidificado Tamponado (AAT) e Reação em Cadeia da Polimerase (PCR), mediante resultado molecular negativo para brucelose optou-se por análises microbiológicas. Os exames sorológicos de parte das amostras foram positivos para brucelose, porém todas apresentaram resultados negativos à PCR. No cultivo bacteriano foram isoladas as espécies Streptococcus equi subsp. zooepidemicus, Enterococcus faecalis e Staphylococcus sciuri subsp. lentus., os quais apresentaram resistência aos antibacterianos, em especial o isolado de E. faecalis classificado como MDR (resistência múltipla a drogas). Concluindo-se que a melhor forma de diagnóstico é a avaliação conjunta de diversos testes, tanto de triagem como confirmatórios, a fim de se obter um diagnóstico definitivo com assertividade.Research, Society and Development2021-07-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1775410.33448/rsd-v10i9.17754Research, Society and Development; Vol. 10 No. 9; e34910917754Research, Society and Development; Vol. 10 Núm. 9; e34910917754Research, Society and Development; v. 10 n. 9; e349109177542525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/17754/16226Copyright (c) 2021 Vitória Fonseca Rodrigues; Andrea Viana da Cruz; Elem Cristina Macedo Barra; Barbara Wilka Leal Silva; Camila de Cássia dos Santos; Keila Feitosa Valente; Thamillys Rayssa Marques Monteiro; Djacy Barbosa Ribeiro; Cintya de Oliveira Souza; Naiana Fleury da Fonseca Ampuero; Loreno da Costa Francez; Ana Judith Pires Garcia Quaresma; Andréa Maria Góes Negrão; Lívia Medeiros Neves Casseb; Alexandre do Rosário Cassebhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRodrigues, Vitória FonsecaCruz, Andrea Viana daBarra, Elem Cristina MacedoSilva, Barbara Wilka LealSantos, Camila de Cássia dosValente, Keila FeitosaMonteiro, Thamillys Rayssa MarquesRibeiro, Djacy BarbosaSouza, Cintya de OliveiraAmpuero, Naiana Fleury da FonsecaFrancez, Loreno da CostaQuaresma, Ana Judith Pires GarciaNegrão, Andréa Maria Góes Casseb, Lívia Medeiros NevesCasseb, Alexandre do Rosário2021-09-12T14:28:06Zoai:ojs.pkp.sfu.ca:article/17754Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:38:02.222161Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Microbiological, serological and molecular diagnosis of abscess of withers in equine in the State of Pará Diagnóstico microbiológico, serológico y molecular de los abscesos en cruz de caballo en el Estado de Pará Diagnóstico microbiológico, sorológico e molecular de abscessos de cernelha em equinos no Estado do Pará |
title |
Microbiological, serological and molecular diagnosis of abscess of withers in equine in the State of Pará |
spellingShingle |
Microbiological, serological and molecular diagnosis of abscess of withers in equine in the State of Pará Rodrigues, Vitória Fonseca Brucelose Brucella melitensis Equino Doença zoonótica. Brucelosis Brucella melitensis Equino Enfermedad zoonótica. Brucellosis Brucella melitensis Equine Zoonotic disease. |
title_short |
Microbiological, serological and molecular diagnosis of abscess of withers in equine in the State of Pará |
title_full |
Microbiological, serological and molecular diagnosis of abscess of withers in equine in the State of Pará |
title_fullStr |
Microbiological, serological and molecular diagnosis of abscess of withers in equine in the State of Pará |
title_full_unstemmed |
Microbiological, serological and molecular diagnosis of abscess of withers in equine in the State of Pará |
title_sort |
Microbiological, serological and molecular diagnosis of abscess of withers in equine in the State of Pará |
author |
Rodrigues, Vitória Fonseca |
author_facet |
Rodrigues, Vitória Fonseca Cruz, Andrea Viana da Barra, Elem Cristina Macedo Silva, Barbara Wilka Leal Santos, Camila de Cássia dos Valente, Keila Feitosa Monteiro, Thamillys Rayssa Marques Ribeiro, Djacy Barbosa Souza, Cintya de Oliveira Ampuero, Naiana Fleury da Fonseca Francez, Loreno da Costa Quaresma, Ana Judith Pires Garcia Negrão, Andréa Maria Góes Casseb, Lívia Medeiros Neves Casseb, Alexandre do Rosário |
author_role |
author |
author2 |
Cruz, Andrea Viana da Barra, Elem Cristina Macedo Silva, Barbara Wilka Leal Santos, Camila de Cássia dos Valente, Keila Feitosa Monteiro, Thamillys Rayssa Marques Ribeiro, Djacy Barbosa Souza, Cintya de Oliveira Ampuero, Naiana Fleury da Fonseca Francez, Loreno da Costa Quaresma, Ana Judith Pires Garcia Negrão, Andréa Maria Góes Casseb, Lívia Medeiros Neves Casseb, Alexandre do Rosário |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigues, Vitória Fonseca Cruz, Andrea Viana da Barra, Elem Cristina Macedo Silva, Barbara Wilka Leal Santos, Camila de Cássia dos Valente, Keila Feitosa Monteiro, Thamillys Rayssa Marques Ribeiro, Djacy Barbosa Souza, Cintya de Oliveira Ampuero, Naiana Fleury da Fonseca Francez, Loreno da Costa Quaresma, Ana Judith Pires Garcia Negrão, Andréa Maria Góes Casseb, Lívia Medeiros Neves Casseb, Alexandre do Rosário |
dc.subject.por.fl_str_mv |
Brucelose Brucella melitensis Equino Doença zoonótica. Brucelosis Brucella melitensis Equino Enfermedad zoonótica. Brucellosis Brucella melitensis Equine Zoonotic disease. |
topic |
Brucelose Brucella melitensis Equino Doença zoonótica. Brucelosis Brucella melitensis Equino Enfermedad zoonótica. Brucellosis Brucella melitensis Equine Zoonotic disease. |
description |
There are several infectious diseases that affect equines and can cause large economic losses in this sector and damage to animal welfare. In this species, abscessing lesions of a purulent character preferentially in the withers are strongly suggestive of brucellosis. However, few studies have been carried out on the disease in this species, making its identification and control difficult due to the lack of standardization in the interpretation of results. Therefore, samples of whole blood, serum and purulent secretion were collected from three horses. The animals came from the state of Pará. The diagnostic methods selected were the Rose-Bengal plate test (RBPT) and Polymerase Chain Reaction (PCR), for the negative molecular results for brucellosis, microbiological analysis were chosen. Although part of the samples were positive for brucellosis in serological tests, all of them showed negative results for PCR. In bacterial cultivation, species were isolated Streptococcus equi subsp. zooepidemicus, Enterococcus faecalis e Staphylococcus sciuri subsp. lentus., which showed resistance to antibacterials, especially the E. faecalis isolate classified as MDR (multi drug resistant). In conclusion, the best form of diagnosis is the joint evaluation of several tests, both screening and confirmatory, in order to obtain a definitive diagnosis with assertiveness. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/17754 10.33448/rsd-v10i9.17754 |
url |
https://rsdjournal.org/index.php/rsd/article/view/17754 |
identifier_str_mv |
10.33448/rsd-v10i9.17754 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/17754/16226 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 9; e34910917754 Research, Society and Development; Vol. 10 Núm. 9; e34910917754 Research, Society and Development; v. 10 n. 9; e34910917754 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052752232185856 |