Serological diagnosis of Chagas disease: Evaluation of cross reaction in patients with visceral leishmaniasis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/14597 |
Resumo: | In Brazil, there are several simultaneously endemic regions for leishmaniasis and Chagas disease and, as the causative agents both belong to the same family, Trypanosomatidae, they share several antigens, which at the time of serological diagnosis can promote cross reactions. Several authors have evaluated Chagas/leishmaniasis cross reaction in dogs, however few studies have made this question in humans. The objective of this study was to identify the possibility of cross reaction for the diagnosis of Chagas disease in patients with cured visceral leishmaniasis. Therefore, thirty serum samples from patients with cured visceral leishmaniasis at the Infectious Diseases Clinic of the Hospital Regional de Presidente Prudente, São Paulo were analyzed. The samples were evaluated using three serological kits for the diagnosis of Chagas disease: immunoenzymatic assay (ELISA), indirect immunofluorescence (IFI) and indirect hemagglutination (HAI). Of the 30 samples analyzed, only one patient's serum showed to be reagent in the IFI, however, the duplicate evaluated was not positive, as well as the ELISA and HAI results. In the present study, due to the reactivity for the IFI to present in only one marked T. cruzi, with its duplicate and other serological tests (ELISA and HAI) negative, we can conclude that the patient with visceral leishmaniasis presented a cross reaction for the diagnosis of Chagas disease. |
| id |
UNIFEI_d6d2de0eb1ca3a10ad5f5a27024f86f7 |
|---|---|
| oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/14597 |
| network_acronym_str |
UNIFEI |
| network_name_str |
Research, Society and Development |
| repository_id_str |
|
| spelling |
Serological diagnosis of Chagas disease: Evaluation of cross reaction in patients with visceral leishmaniasisDiagnóstico serológico de la enfermedad de Chagas: Evaluación de la reacción cruzada en pacientes con leishmaniasis visceralDiagnóstico da doença de Chagas: Avaliação da reação cruzada em pacientes com leishmaniose visceralTrypanosoma cruziLeishmaniaTestes sorológicosReações cruzadas.Trypanosoma cruziLeishmaniaPruebas serológicasReacciones cruzadas.Trypanosoma cruziLeishmaniaSerologic testsCross reactions.In Brazil, there are several simultaneously endemic regions for leishmaniasis and Chagas disease and, as the causative agents both belong to the same family, Trypanosomatidae, they share several antigens, which at the time of serological diagnosis can promote cross reactions. Several authors have evaluated Chagas/leishmaniasis cross reaction in dogs, however few studies have made this question in humans. The objective of this study was to identify the possibility of cross reaction for the diagnosis of Chagas disease in patients with cured visceral leishmaniasis. Therefore, thirty serum samples from patients with cured visceral leishmaniasis at the Infectious Diseases Clinic of the Hospital Regional de Presidente Prudente, São Paulo were analyzed. The samples were evaluated using three serological kits for the diagnosis of Chagas disease: immunoenzymatic assay (ELISA), indirect immunofluorescence (IFI) and indirect hemagglutination (HAI). Of the 30 samples analyzed, only one patient's serum showed to be reagent in the IFI, however, the duplicate evaluated was not positive, as well as the ELISA and HAI results. In the present study, due to the reactivity for the IFI to present in only one marked T. cruzi, with its duplicate and other serological tests (ELISA and HAI) negative, we can conclude that the patient with visceral leishmaniasis presented a cross reaction for the diagnosis of Chagas disease.En Brasil, existen varias regiones endémicas simultáneamente para la leishmaniasis y la enfermedad de Chagas y, como los agentes causales pertenecen a la misma familia, Trypanosomatidae, comparten varios antígenos, que en el momento del diagnóstico serológico pueden promover reacciones cruzadas. Varios autores han evaluado la reacción cruzada de Chagas / leishmaniasis en perros, sin embargo pocos estudios han hecho esta pregunta en humanos. El objetivo de este estudio fue identificar la posibilidad de reacción cruzada para el diagnóstico de la enfermedad de Chagas en pacientes con leishmaniasis visceral curada. Por lo tanto, se analizaron treinta muestras de suero de pacientes con leishmaniasis visceral curada en la Clínica de Enfermedades Infecciosas del Hospital Regional de Presidente Prudente, São Paulo. Las muestras se evaluaron mediante tres kits serológicos para el diagnóstico de la enfermedad de Chagas: ensayo inmunoenzimático (ELISA), inmunofluorescencia indirecta (IFI) y hemaglutinación indirecta (HAI). De las 30 muestras analizadas, solo el suero de un paciente mostró ser reactivo en el IFI, sin embargo, el duplicado evaluado no fue positivo, así como los resultados de ELISA y HAI. En el presente estudio, debido a la reactividad para que el IFI se presente en un solo T. cruzi marcado, con su duplicado y otras pruebas serológicas (ELISA y HAI) negativas, podemos concluir que el paciente con leishmaniasis visceral presentó una reacción cruzada para el diagnóstico de la enfermedad de Chagas.No Brasil, existem diversas regiões endêmicas simultaneamente para leishmaniose e doença de Chagas e como os agentes causadores pertencem ambos à mesma família, Trypanosomatidae, eles compartilham diversos antígenos, que no momento do diagnóstico sorológico, podem promover reações cruzadas. Diversos autores avaliaram a reação cruzada Chagas/leishmaniose em cães, entretanto poucos trabalhos fizeram este questionamento em humanos. O objetivo do presente estudo foi identificar a possibilidade de reação cruzada para o diagnóstico da doença de Chagas em pacientes com leishmaniose visceral curada. Para tanto, foram analisadas 30 amostras de soro de pacientes com leishmaniose visceral curada atendidos no Ambulatório de Infectologia do Hospital Regional de Presidente Prudente, São Paulo. As amostras foram avaliadas em duplicata através de três kits sorológicos para o diagnóstico da doença de Chagas: ensaio imunoenzimático (ELISA), imunofluorescência indireta (IFI) e hemaglutinação indireta (HAI). Das 30 amostras analisadas, apenas o soro de um paciente mostrou-se reagente na IFI, entretanto, a duplicata avaliada não foi positiva, assim como os resultados de ELISA e HAI. No presente estudo, devido à reatividade para a IFI apresentar apenas um T. cruzi marcado, com a sua duplicata e outros testes sorológicos (ELISA e HAI) negativos, podemos concluir que o paciente com leishmaniose visceral apresentou uma reação cruzada para o diagnóstico da Doença de Chagas.Research, Society and Development2021-04-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1459710.33448/rsd-v10i4.14597Research, Society and Development; Vol. 10 No. 4; e55910414597Research, Society and Development; Vol. 10 Núm. 4; e55910414597Research, Society and Development; v. 10 n. 4; e559104145972525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/14597/13008Copyright (c) 2021 Lucas Silva Barcelos; Amanda Aparecida Silva de Aguiar; Fernando Nunes Gavioli Boni; Luiz Euribel Prestes Carneiro; Thaís Batista de Carvalho; Eliana Peresi-Lordelohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBarcelos, Lucas Silva Aguiar, Amanda Aparecida Silva de Boni, Fernando Nunes Gavioli Carneiro, Luiz Euribel Prestes Carvalho, Thaís Batista de Peresi-Lordelo, Eliana2021-04-25T11:21:26Zoai:ojs.pkp.sfu.ca:article/14597Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:35:40.661965Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Serological diagnosis of Chagas disease: Evaluation of cross reaction in patients with visceral leishmaniasis Diagnóstico serológico de la enfermedad de Chagas: Evaluación de la reacción cruzada en pacientes con leishmaniasis visceral Diagnóstico da doença de Chagas: Avaliação da reação cruzada em pacientes com leishmaniose visceral |
| title |
Serological diagnosis of Chagas disease: Evaluation of cross reaction in patients with visceral leishmaniasis |
| spellingShingle |
Serological diagnosis of Chagas disease: Evaluation of cross reaction in patients with visceral leishmaniasis Barcelos, Lucas Silva Trypanosoma cruzi Leishmania Testes sorológicos Reações cruzadas. Trypanosoma cruzi Leishmania Pruebas serológicas Reacciones cruzadas. Trypanosoma cruzi Leishmania Serologic tests Cross reactions. |
| title_short |
Serological diagnosis of Chagas disease: Evaluation of cross reaction in patients with visceral leishmaniasis |
| title_full |
Serological diagnosis of Chagas disease: Evaluation of cross reaction in patients with visceral leishmaniasis |
| title_fullStr |
Serological diagnosis of Chagas disease: Evaluation of cross reaction in patients with visceral leishmaniasis |
| title_full_unstemmed |
Serological diagnosis of Chagas disease: Evaluation of cross reaction in patients with visceral leishmaniasis |
| title_sort |
Serological diagnosis of Chagas disease: Evaluation of cross reaction in patients with visceral leishmaniasis |
| author |
Barcelos, Lucas Silva |
| author_facet |
Barcelos, Lucas Silva Aguiar, Amanda Aparecida Silva de Boni, Fernando Nunes Gavioli Carneiro, Luiz Euribel Prestes Carvalho, Thaís Batista de Peresi-Lordelo, Eliana |
| author_role |
author |
| author2 |
Aguiar, Amanda Aparecida Silva de Boni, Fernando Nunes Gavioli Carneiro, Luiz Euribel Prestes Carvalho, Thaís Batista de Peresi-Lordelo, Eliana |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Barcelos, Lucas Silva Aguiar, Amanda Aparecida Silva de Boni, Fernando Nunes Gavioli Carneiro, Luiz Euribel Prestes Carvalho, Thaís Batista de Peresi-Lordelo, Eliana |
| dc.subject.por.fl_str_mv |
Trypanosoma cruzi Leishmania Testes sorológicos Reações cruzadas. Trypanosoma cruzi Leishmania Pruebas serológicas Reacciones cruzadas. Trypanosoma cruzi Leishmania Serologic tests Cross reactions. |
| topic |
Trypanosoma cruzi Leishmania Testes sorológicos Reações cruzadas. Trypanosoma cruzi Leishmania Pruebas serológicas Reacciones cruzadas. Trypanosoma cruzi Leishmania Serologic tests Cross reactions. |
| description |
In Brazil, there are several simultaneously endemic regions for leishmaniasis and Chagas disease and, as the causative agents both belong to the same family, Trypanosomatidae, they share several antigens, which at the time of serological diagnosis can promote cross reactions. Several authors have evaluated Chagas/leishmaniasis cross reaction in dogs, however few studies have made this question in humans. The objective of this study was to identify the possibility of cross reaction for the diagnosis of Chagas disease in patients with cured visceral leishmaniasis. Therefore, thirty serum samples from patients with cured visceral leishmaniasis at the Infectious Diseases Clinic of the Hospital Regional de Presidente Prudente, São Paulo were analyzed. The samples were evaluated using three serological kits for the diagnosis of Chagas disease: immunoenzymatic assay (ELISA), indirect immunofluorescence (IFI) and indirect hemagglutination (HAI). Of the 30 samples analyzed, only one patient's serum showed to be reagent in the IFI, however, the duplicate evaluated was not positive, as well as the ELISA and HAI results. In the present study, due to the reactivity for the IFI to present in only one marked T. cruzi, with its duplicate and other serological tests (ELISA and HAI) negative, we can conclude that the patient with visceral leishmaniasis presented a cross reaction for the diagnosis of Chagas disease. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-04-23 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/14597 10.33448/rsd-v10i4.14597 |
| url |
https://rsdjournal.org/index.php/rsd/article/view/14597 |
| identifier_str_mv |
10.33448/rsd-v10i4.14597 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/14597/13008 |
| dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Research, Society and Development |
| publisher.none.fl_str_mv |
Research, Society and Development |
| dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 4; e55910414597 Research, Society and Development; Vol. 10 Núm. 4; e55910414597 Research, Society and Development; v. 10 n. 4; e55910414597 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
| instname_str |
Universidade Federal de Itajubá (UNIFEI) |
| instacron_str |
UNIFEI |
| institution |
UNIFEI |
| reponame_str |
Research, Society and Development |
| collection |
Research, Society and Development |
| repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
| repository.mail.fl_str_mv |
rsd.articles@gmail.com |
| _version_ |
1797052675460694016 |