Baccharis dracunculifolia DC (Asteraceae) leaf and flower essential oils to control Rhipicephalus microplus Canestrini (Arachnida: Ixodidae) in the free-living stage

• Autor(a) principal: Cazella, Luciane Neris
• Data de Publicação: 2020
• Outros Autores: Oliveira, Herika Line de Marko de, Bortolucci, Wanessa de Campos, Rahal, Isabelle Luiz, Baretta, Irinéia Paulina, Gonçalves, José Eduardo, Piau Junior, Ranulfo, Fernandez, Carla Maria Mariano, Linde, Giani Andrea, Colauto, Nelson Barros, Gazim, Zilda Cristiani
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Research, Society and Development
• Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/8788
• topic: Wild rosemary; Cattle tick; Nerolidol; Spathulenol; Ex situ test.; Alecrim-do-campo; Garrapata del ganado; Nerolidol; Espatulenol; Ensayo ex situ.; Alecrim-do-campo; Carrapato bovino; Nerolidol; Espatulenol; Ex situ teste.

Baccharis dracunculifolia, native to Brazil and the main source of “green propolis”, has been reported with several biological activities, and may be a source of bovine tick control substituting synthetic acaricides. Objective: to evaluate the in vitro and ex situ acaricidal activity of B. dracunculifolia leaf and flower essential oils against Rhipicephalus microplus. Methodology: the essential oils were extracted by hydrodistillation and analyzed by a gas chromatography coupled to mass spectrometry; the acaricidal activity of the essential oil was evaluated in vitro against adult females and against the egg hatchability; moreover, the acaricidal activity against tick larvae was evaluated in vitro and ex situ. Results: the major class of the essential oils was oxygenated sesquiterpene (55.1% leaves 50.4% flowers) and the main compounds were (21.5% leaves; 20.6% flowers) and spathulenol (21.8% leaves; 20.3% flowers). The essential oil at 500 mg/mL was effective to control egg hatchability with a reduction of egg laying capacity and decrease of number of adult ticks and larvae. The larvicidal activity of the essential oil had LC99.9 from 35 to 37 mg/mL by probit analysis, and the essential oil from 11 to 14 mg/mL presented 85 to 95% of treatment efficiency in the ex situ test. Conclusion: B. dracunculifolia leaf and flower essential oils are stable and have application potential to control bovine ticks.