Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changes
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/29387 |
Resumo: | Currently, the commercial production of chitosan takes place through the thermochemical deacetylation of crustacean shells, which process requires the use of chemical substances, such as strong alkaline solutions, which are important sources of environment pollution. We present a method for the production of chitosan by Cunninghamella elegans UCP 1306 using a green metabolic conversion of agro-industrial residues, based on a 22 Factorial Design and describe the main morphological changes observed in the specie. The highest yield of biomass (6.375 g/L) and chitosan (101.7 mg/g) were observed in the assay 2 (Corn steep liquor -CSL 4%, Cassava wastewater-CWW 4%), where the specie presented loose and branched hyphae with the presence of septations, pellets of 0.4 to 0.5 mm and clumps of up to 0.1 mm. Statistical analysis showed that higher concentration of CSL contributed significantly to the growth of the species. The bands determined in Fourier transform infrared spectroscopy confirmed the degree of deacetylation of 84.61% of the chitosan obtained. This research showed that the use of CSL and CWW in association was very promising, being able to be used as sustainable sources of nitrogen and carbon in the production of fungal chitosan with a high degree of deacetylation and can be applied to the industrial process. |
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Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changesSíntesis verde de quitosano por Cunninghamella elegans UCP 1306 utilizando sustratos sostenibles mediados por cambios morfológicosSíntese verde de quitosana por Cunninghamella elegans UCP 1306 usando substratos sustentáveis mediados por mudanças morfológicas Resíduos agroindustriaisBiopolímerosQuitosana fúngicaCunninghamella elegans.Residuos agroindustrialesBiopolímerosQuitosano fúngicoCunninghamella elegans.Agroindustrial wasteBiopolymersFungal ChitosanCunninghamella elegans.Currently, the commercial production of chitosan takes place through the thermochemical deacetylation of crustacean shells, which process requires the use of chemical substances, such as strong alkaline solutions, which are important sources of environment pollution. We present a method for the production of chitosan by Cunninghamella elegans UCP 1306 using a green metabolic conversion of agro-industrial residues, based on a 22 Factorial Design and describe the main morphological changes observed in the specie. The highest yield of biomass (6.375 g/L) and chitosan (101.7 mg/g) were observed in the assay 2 (Corn steep liquor -CSL 4%, Cassava wastewater-CWW 4%), where the specie presented loose and branched hyphae with the presence of septations, pellets of 0.4 to 0.5 mm and clumps of up to 0.1 mm. Statistical analysis showed that higher concentration of CSL contributed significantly to the growth of the species. The bands determined in Fourier transform infrared spectroscopy confirmed the degree of deacetylation of 84.61% of the chitosan obtained. This research showed that the use of CSL and CWW in association was very promising, being able to be used as sustainable sources of nitrogen and carbon in the production of fungal chitosan with a high degree of deacetylation and can be applied to the industrial process.Actualmente, la producción comercial de quitosano se realiza a través del proceso de desacetilación termoquímica de caparazones de crustáceos, cuyo proceso requiere el uso de sustancias químicas, como soluciones alcalinas fuertes, las cuales son fuentes importantes de contaminación para el medio ambiente. El presente estudio presenta un método para la producción de quitosano por la especie Cunninghamella elegans (UCP 1306) utilizando una conversión metabólica verde con residuos agroindustriales, basado en un Diseño Factorial 2² y se describen los principales cambios morfológicos observados en la especie. El mayor rendimiento de biomasa (6,375 g/L) y quitosano (101,7 mg/g) se observó en el ensayo 2 (licor de maceración de maíz- CSL 4% y agua residual de yuca- CWW 4%), donde las especies presentaron predominio de hojas sueltas y ramificadas. hifas con presencia de tabicaciones, gránulos de 0,4 a 0,5 mm y macizos de hasta 0,1 mm. El análisis estadístico mostró que la concentración más alta de CSL contribuyó significativamente al crecimiento de la especie. Las bandas determinadas por espectroscopia infrarroja transformada de Fourier confirmaron el grado de desacetilación del 84,61% del quitosano obtenida. Esta investigación mostró que la asociación de CSL y CWW fue muy prometedora, pudiéndose utilizar como fuentes sostenibles de carbono y nitrógeno en la producción de quitosano fúngico con un alto grado de desacetilación y puede aplicarse al proceso industrial.Atualmente, a produção comercial de quitosana se dá pelo processo de desacetilação termoquímica a partir de carapaças de crustáceos, cujo processo requer o uso de substâncias químicas, como soluções alcalinas fortes, que constituem importantes fontes de poluição para o meio ambiente. O presente estudo apresenta um método para a produção de quitosana pela espécie Cunninghamella elegans (UCP 1306) usando uma conversão metabólica verde com resíduos agroindustrais, baseado em um Planejamento Fatorial 2² e descrevemos as principais alterações morfológicas observadas na espécie. O maior rendimento de biomassa (6,375 g / L) e quitosana (101,7 mg/g) foi observado no ensaio 2 (licor de maceração de milho-CSL 4% e água residual de mandioca- CWW 4%), onde a espécie apresentou predominância de hifas frouxas e ramificadas com presença de septações, pellets de 0,4 a 0,5 mm e clumps de até 0,1 mm. A análise estatística mostrou que a maior concentração de CSL contribuiu significativamente para o crescimento da espécie. As bandas determinadas na espectroscopia no infravermelho com transformada de Fourier confirmaram o grau de desacetilação de 84,61% da quitosana obtida. Esta pesquisa mostrou que a associação de CSL e CWW foi bastante promissora, podendo ser utilizadas como fontes sustentáveis de carbono e nitrogênio na produção de quitosana fúngica com alto grau de desacetilação e pode ser aplicada ao processo industrial.Research, Society and Development2022-05-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2938710.33448/rsd-v11i7.29387Research, Society and Development; Vol. 11 No. 7; e38211729387Research, Society and Development; Vol. 11 Núm. 7; e38211729387Research, Society and Development; v. 11 n. 7; e382117293872525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/29387/25970Copyright (c) 2022 Ákylla Fernanda Souza Silva; Adriana Ferreira de Souza; Irapuan Oliveira Pinheiro; Galba Maria de Campos-Takakihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva, Ákylla Fernanda SouzaSouza, Adriana Ferreira dePinheiro, Irapuan Oliveira Campos-Takaki, Galba Maria de 2022-06-06T15:12:05Zoai:ojs.pkp.sfu.ca:article/29387Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:46:29.130810Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changes Síntesis verde de quitosano por Cunninghamella elegans UCP 1306 utilizando sustratos sostenibles mediados por cambios morfológicos Síntese verde de quitosana por Cunninghamella elegans UCP 1306 usando substratos sustentáveis mediados por mudanças morfológicas |
title |
Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changes |
spellingShingle |
Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changes Silva, Ákylla Fernanda Souza Resíduos agroindustriais Biopolímeros Quitosana fúngica Cunninghamella elegans. Residuos agroindustriales Biopolímeros Quitosano fúngico Cunninghamella elegans. Agroindustrial waste Biopolymers Fungal Chitosan Cunninghamella elegans. |
title_short |
Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changes |
title_full |
Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changes |
title_fullStr |
Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changes |
title_full_unstemmed |
Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changes |
title_sort |
Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changes |
author |
Silva, Ákylla Fernanda Souza |
author_facet |
Silva, Ákylla Fernanda Souza Souza, Adriana Ferreira de Pinheiro, Irapuan Oliveira Campos-Takaki, Galba Maria de |
author_role |
author |
author2 |
Souza, Adriana Ferreira de Pinheiro, Irapuan Oliveira Campos-Takaki, Galba Maria de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Silva, Ákylla Fernanda Souza Souza, Adriana Ferreira de Pinheiro, Irapuan Oliveira Campos-Takaki, Galba Maria de |
dc.subject.por.fl_str_mv |
Resíduos agroindustriais Biopolímeros Quitosana fúngica Cunninghamella elegans. Residuos agroindustriales Biopolímeros Quitosano fúngico Cunninghamella elegans. Agroindustrial waste Biopolymers Fungal Chitosan Cunninghamella elegans. |
topic |
Resíduos agroindustriais Biopolímeros Quitosana fúngica Cunninghamella elegans. Residuos agroindustriales Biopolímeros Quitosano fúngico Cunninghamella elegans. Agroindustrial waste Biopolymers Fungal Chitosan Cunninghamella elegans. |
description |
Currently, the commercial production of chitosan takes place through the thermochemical deacetylation of crustacean shells, which process requires the use of chemical substances, such as strong alkaline solutions, which are important sources of environment pollution. We present a method for the production of chitosan by Cunninghamella elegans UCP 1306 using a green metabolic conversion of agro-industrial residues, based on a 22 Factorial Design and describe the main morphological changes observed in the specie. The highest yield of biomass (6.375 g/L) and chitosan (101.7 mg/g) were observed in the assay 2 (Corn steep liquor -CSL 4%, Cassava wastewater-CWW 4%), where the specie presented loose and branched hyphae with the presence of septations, pellets of 0.4 to 0.5 mm and clumps of up to 0.1 mm. Statistical analysis showed that higher concentration of CSL contributed significantly to the growth of the species. The bands determined in Fourier transform infrared spectroscopy confirmed the degree of deacetylation of 84.61% of the chitosan obtained. This research showed that the use of CSL and CWW in association was very promising, being able to be used as sustainable sources of nitrogen and carbon in the production of fungal chitosan with a high degree of deacetylation and can be applied to the industrial process. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/29387 10.33448/rsd-v11i7.29387 |
url |
https://rsdjournal.org/index.php/rsd/article/view/29387 |
identifier_str_mv |
10.33448/rsd-v11i7.29387 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/29387/25970 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 7; e38211729387 Research, Society and Development; Vol. 11 Núm. 7; e38211729387 Research, Society and Development; v. 11 n. 7; e38211729387 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052765872062464 |