Comparison among direct evaluation methods of babesiosis diagnosis in cattle
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/1388 |
Resumo: | The objective of the present study was to compare three methods of direct observation: blood smear, ear tip smear and leucoplatelet cover, searching for the best method for the diagnosis of Babesia spp. in cattle. Seventy-seven bovines with suspected babesiosis were used. From these animals, blood was collected in jugular vein and ear tip. After obtaining the samples, the blood smears and the leucoplaquetary layer were made with material from the jugular and smear of the ear tip with material from the perforation of the ear tip. Of the 77 animals, 14,30% (n = 11) inclusions were found in at least one of the three evaluated methods. Of the positive animals, 36.35% (n = 4) were positive only in the blood smear and the ear tip smear alone was not able to diagnose the disease in any of the animals. The concordance coefficient obtained in the kappa test showed a poor reconciliation between the blood smear observation method, considered gold standard in the literature, with blood smear (k = 0.18) and with the leucoplatelet layer (k = -0, 12). The sensitivity and specificity of the blood smear was 40% and 100% and the leucoplatelet layer obtained 25% and 62%, respectively. The blood smear with blood obtained from the jugular was the method with the best results when compared to the other techniques for the diagnosis of babesiosis in cattle. |
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Comparison among direct evaluation methods of babesiosis diagnosis in cattleComparación entre los métodos de evaluación directa para el diagnóstico de babesiosis en bovinosComparação entre métodos de avaliação direta para o diagnóstico de babesiose em bovinosHemoparasitasEsfregaço sanguíneoTristeza Parasitária Bovina.HemoparasitasFrotis sanguíneoTristeza Parasitaria Bovina.HemoparasitesBlood smearBovine Parasitic Sadness.The objective of the present study was to compare three methods of direct observation: blood smear, ear tip smear and leucoplatelet cover, searching for the best method for the diagnosis of Babesia spp. in cattle. Seventy-seven bovines with suspected babesiosis were used. From these animals, blood was collected in jugular vein and ear tip. After obtaining the samples, the blood smears and the leucoplaquetary layer were made with material from the jugular and smear of the ear tip with material from the perforation of the ear tip. Of the 77 animals, 14,30% (n = 11) inclusions were found in at least one of the three evaluated methods. Of the positive animals, 36.35% (n = 4) were positive only in the blood smear and the ear tip smear alone was not able to diagnose the disease in any of the animals. The concordance coefficient obtained in the kappa test showed a poor reconciliation between the blood smear observation method, considered gold standard in the literature, with blood smear (k = 0.18) and with the leucoplatelet layer (k = -0, 12). The sensitivity and specificity of the blood smear was 40% and 100% and the leucoplatelet layer obtained 25% and 62%, respectively. The blood smear with blood obtained from the jugular was the method with the best results when compared to the other techniques for the diagnosis of babesiosis in cattle.El objetivo del presente estudio fue comparar tres métodos de observación directa: frotis sanguíneo, frotis de punta de oreja y capa leucoplaquetaria, buscando el mejor método para el diagnóstico de Babesia spp. en bovinos. Se utilizaron 77 bovinos con sospechosos de babesiosis. De estos animales, se realizó la recolección de sangre en vena yugular y punta de oreja. Después de la obtención de las muestras, se confeccionaron los frotis sanguíneos y capa leucoplaquetaria con material proveniente de la yugular y frotis de punta de oreja con material proveniente de la perforación de la punta de oreja. Dos 77 animales, 14, 30% (n = 11) se encontraron inclusiones en al menos uno de los tres métodos evaluados. De los animales positivos, 36,35% (n = 4) fueron positivos sólo en el frotis sanguíneo y el frotis de punta de oreja solo no fue capaz de diagnosticar la enfermedad en ninguno de los animales. El coeficiente de concordancia obtenido en la prueba kappa mostró una débil conciliación entre el método de observación por el frotis de sangre de punta de oreja considerado patrón oro por la literatura, con frotis sanguíneo (k = 0,18) y con la capa leucoplaquetaria (k = -0,12). La sensibilidad y especificidad del frotis sanguíneo fue del 40% y 100% y la capa leucoplaquetaria obtuvo el 25% y el 62%, respectivamente. El frotis sanguíneo con sangre obtenida de la yugular, se mostró el método con mejores resultados, cuando comparado a las demás técnicas, para el diagnóstico de babesiosis en bovinos.O objetivo do presente estudo foi comparar três métodos de observação direta: esfregaço sanguíneo, esfregaço de ponta de orelha e capa leucoplaquetária, buscando o melhor método para o diagnóstico de Babesia spp. em bovinos. Foram utilizados 77 bovinos com suspeita de babesiose. Destes animais, foi realizada a coleta de sangue em veia jugular e ponta de orelha. Após obtenção das amostras, foram confeccionados os esfregaços sanguíneos e capa leucoplaquetária com material proveniente da jugular e esfregaço de ponta de orelha com material proveniente da perfuração da ponta de orelha. Dos 77 animais, 14,30% (n=11) foram encontradas inclusões em pelo menos um dos três métodos avaliados. Dos animais positivos, 36,35% (n=4) foram positivos apenas no esfregaço sanguíneo e o esfregaço de ponta de orelha sozinho não foi capaz de diagnosticar a enfermidade em nenhum dos animais. O coeficiente de concordância obtido no teste kappa mostrou fraca conciliação entre o método de observação pelo esfregaço de sangue de ponta de orelha considerado padrão ouro pela literatura, com esfregaço sanguíneo (k= 0,18) e com a capa leucoplaquetária (k = -0,12). A sensibilidade e especificidade do esfregaço sanguíneo foi de 40% e 100% e capa leucoplaquetária obteve 25% e 62%, respectivamente. O esfregaço sanguíneo com sangue obtido da jugular, mostrou-se o método com melhores resultados, quando comparado às demais técnicas, para o diagnóstico de babesiose em bovinos.Research, Society and Development2019-08-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/138810.33448/rsd-v8i10.1388Research, Society and Development; Vol. 8 No. 10; e328101388Research, Society and Development; Vol. 8 Núm. 10; e328101388Research, Society and Development; v. 8 n. 10; e3281013882525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/1388/1113Copyright (c) 2019 Lorena Silveira de Almeida, Larissa Marchiori Sena, Graziela Barioni, Théo Matos Arantes Moraes, Ronaldo Eugênio de Oliveirainfo:eu-repo/semantics/openAccessAlmeida, Lorena Silveira deSena, Larissa MarchioriBarioni, GrazielaMoraes, Théo Matos ArantesOliveira, Ronaldo Eugênio de2020-03-25T16:06:52Zoai:ojs.pkp.sfu.ca:article/1388Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:26:26.534354Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Comparison among direct evaluation methods of babesiosis diagnosis in cattle Comparación entre los métodos de evaluación directa para el diagnóstico de babesiosis en bovinos Comparação entre métodos de avaliação direta para o diagnóstico de babesiose em bovinos |
title |
Comparison among direct evaluation methods of babesiosis diagnosis in cattle |
spellingShingle |
Comparison among direct evaluation methods of babesiosis diagnosis in cattle Almeida, Lorena Silveira de Hemoparasitas Esfregaço sanguíneo Tristeza Parasitária Bovina. Hemoparasitas Frotis sanguíneo Tristeza Parasitaria Bovina. Hemoparasites Blood smear Bovine Parasitic Sadness. |
title_short |
Comparison among direct evaluation methods of babesiosis diagnosis in cattle |
title_full |
Comparison among direct evaluation methods of babesiosis diagnosis in cattle |
title_fullStr |
Comparison among direct evaluation methods of babesiosis diagnosis in cattle |
title_full_unstemmed |
Comparison among direct evaluation methods of babesiosis diagnosis in cattle |
title_sort |
Comparison among direct evaluation methods of babesiosis diagnosis in cattle |
author |
Almeida, Lorena Silveira de |
author_facet |
Almeida, Lorena Silveira de Sena, Larissa Marchiori Barioni, Graziela Moraes, Théo Matos Arantes Oliveira, Ronaldo Eugênio de |
author_role |
author |
author2 |
Sena, Larissa Marchiori Barioni, Graziela Moraes, Théo Matos Arantes Oliveira, Ronaldo Eugênio de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Almeida, Lorena Silveira de Sena, Larissa Marchiori Barioni, Graziela Moraes, Théo Matos Arantes Oliveira, Ronaldo Eugênio de |
dc.subject.por.fl_str_mv |
Hemoparasitas Esfregaço sanguíneo Tristeza Parasitária Bovina. Hemoparasitas Frotis sanguíneo Tristeza Parasitaria Bovina. Hemoparasites Blood smear Bovine Parasitic Sadness. |
topic |
Hemoparasitas Esfregaço sanguíneo Tristeza Parasitária Bovina. Hemoparasitas Frotis sanguíneo Tristeza Parasitaria Bovina. Hemoparasites Blood smear Bovine Parasitic Sadness. |
description |
The objective of the present study was to compare three methods of direct observation: blood smear, ear tip smear and leucoplatelet cover, searching for the best method for the diagnosis of Babesia spp. in cattle. Seventy-seven bovines with suspected babesiosis were used. From these animals, blood was collected in jugular vein and ear tip. After obtaining the samples, the blood smears and the leucoplaquetary layer were made with material from the jugular and smear of the ear tip with material from the perforation of the ear tip. Of the 77 animals, 14,30% (n = 11) inclusions were found in at least one of the three evaluated methods. Of the positive animals, 36.35% (n = 4) were positive only in the blood smear and the ear tip smear alone was not able to diagnose the disease in any of the animals. The concordance coefficient obtained in the kappa test showed a poor reconciliation between the blood smear observation method, considered gold standard in the literature, with blood smear (k = 0.18) and with the leucoplatelet layer (k = -0, 12). The sensitivity and specificity of the blood smear was 40% and 100% and the leucoplatelet layer obtained 25% and 62%, respectively. The blood smear with blood obtained from the jugular was the method with the best results when compared to the other techniques for the diagnosis of babesiosis in cattle. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-08-23 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/1388 10.33448/rsd-v8i10.1388 |
url |
https://rsdjournal.org/index.php/rsd/article/view/1388 |
identifier_str_mv |
10.33448/rsd-v8i10.1388 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/1388/1113 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 8 No. 10; e328101388 Research, Society and Development; Vol. 8 Núm. 10; e328101388 Research, Society and Development; v. 8 n. 10; e328101388 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052776724824064 |