Comparison among direct evaluation methods of babesiosis diagnosis in cattle

Detalhes bibliográficos
Autor(a) principal: Almeida, Lorena Silveira de
Data de Publicação: 2019
Outros Autores: Sena, Larissa Marchiori, Barioni, Graziela, Moraes, Théo Matos Arantes, Oliveira, Ronaldo Eugênio de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/1388
Resumo: The objective of the present study was to compare three methods of direct observation: blood smear, ear tip smear and leucoplatelet cover, searching for the best method for the diagnosis of Babesia spp. in cattle. Seventy-seven bovines with suspected babesiosis were used. From these animals, blood was collected in jugular vein and ear tip. After obtaining the samples, the blood smears and the leucoplaquetary layer were made with material from the jugular and smear of the ear tip with material from the perforation of the ear tip. Of the 77 animals, 14,30% (n = 11) inclusions were found in at least one of the three evaluated methods. Of the positive animals, 36.35% (n = 4) were positive only in the blood smear and the ear tip smear alone was not able to diagnose the disease in any of the animals. The concordance coefficient obtained in the kappa test showed a poor reconciliation between the blood smear observation method, considered gold standard in the literature, with blood smear (k = 0.18) and with the leucoplatelet layer (k = -0, 12). The sensitivity and specificity of the blood smear was 40% and 100% and the leucoplatelet layer obtained 25% and 62%, respectively. The blood smear with blood obtained from the jugular was the method with the best results when compared to the other techniques for the diagnosis of babesiosis in cattle.
id UNIFEI_d9cc3ae8c386625ebbb18830fa485c0f
oai_identifier_str oai:ojs.pkp.sfu.ca:article/1388
network_acronym_str UNIFEI
network_name_str Research, Society and Development
repository_id_str
spelling Comparison among direct evaluation methods of babesiosis diagnosis in cattleComparación entre los métodos de evaluación directa para el diagnóstico de babesiosis en bovinosComparação entre métodos de avaliação direta para o diagnóstico de babesiose em bovinosHemoparasitasEsfregaço sanguíneoTristeza Parasitária Bovina.HemoparasitasFrotis sanguíneoTristeza Parasitaria Bovina.HemoparasitesBlood smearBovine Parasitic Sadness.The objective of the present study was to compare three methods of direct observation: blood smear, ear tip smear and leucoplatelet cover, searching for the best method for the diagnosis of Babesia spp. in cattle. Seventy-seven bovines with suspected babesiosis were used. From these animals, blood was collected in jugular vein and ear tip. After obtaining the samples, the blood smears and the leucoplaquetary layer were made with material from the jugular and smear of the ear tip with material from the perforation of the ear tip. Of the 77 animals, 14,30% (n = 11) inclusions were found in at least one of the three evaluated methods. Of the positive animals, 36.35% (n = 4) were positive only in the blood smear and the ear tip smear alone was not able to diagnose the disease in any of the animals. The concordance coefficient obtained in the kappa test showed a poor reconciliation between the blood smear observation method, considered gold standard in the literature, with blood smear (k = 0.18) and with the leucoplatelet layer (k = -0, 12). The sensitivity and specificity of the blood smear was 40% and 100% and the leucoplatelet layer obtained 25% and 62%, respectively. The blood smear with blood obtained from the jugular was the method with the best results when compared to the other techniques for the diagnosis of babesiosis in cattle.El objetivo del presente estudio fue comparar tres métodos de observación directa: frotis sanguíneo, frotis de punta de oreja y capa leucoplaquetaria, buscando el mejor método para el diagnóstico de Babesia spp. en bovinos. Se utilizaron 77 bovinos con sospechosos de babesiosis. De estos animales, se realizó la recolección de sangre en vena yugular y punta de oreja. Después de la obtención de las muestras, se confeccionaron los frotis sanguíneos y capa leucoplaquetaria con material proveniente de la yugular y frotis de punta de oreja con material proveniente de la perforación de la punta de oreja. Dos 77 animales, 14, 30% (n = 11) se encontraron inclusiones en al menos uno de los tres métodos evaluados. De los animales positivos, 36,35% (n = 4) fueron positivos sólo en el frotis sanguíneo y el frotis de punta de oreja solo no fue capaz de diagnosticar la enfermedad en ninguno de los animales. El coeficiente de concordancia obtenido en la prueba kappa mostró una débil conciliación entre el método de observación por el frotis de sangre de punta de oreja considerado patrón oro por la literatura, con frotis sanguíneo (k = 0,18) y con la capa leucoplaquetaria (k = -0,12). La sensibilidad y especificidad del frotis sanguíneo fue del 40% y 100% y la capa leucoplaquetaria obtuvo el 25% y el 62%, respectivamente. El frotis sanguíneo con sangre obtenida de la yugular, se mostró el método con mejores resultados, cuando comparado a las demás técnicas, para el diagnóstico de babesiosis en bovinos.O objetivo do presente estudo foi comparar três métodos de observação direta: esfregaço sanguíneo, esfregaço de ponta de orelha e capa leucoplaquetária, buscando o melhor método para o diagnóstico de Babesia spp. em bovinos. Foram utilizados 77 bovinos com suspeita de babesiose.  Destes animais, foi realizada a coleta de sangue em veia jugular e ponta de orelha. Após obtenção das amostras, foram confeccionados os esfregaços sanguíneos e capa leucoplaquetária com material proveniente da jugular e esfregaço de ponta de orelha com material proveniente da perfuração da ponta de orelha. Dos 77 animais, 14,30% (n=11) foram encontradas inclusões em pelo menos um dos três métodos avaliados. Dos animais positivos, 36,35% (n=4) foram positivos apenas no esfregaço sanguíneo e o esfregaço de ponta de orelha sozinho não foi capaz de diagnosticar a enfermidade em nenhum dos animais. O coeficiente de concordância obtido no teste kappa mostrou fraca conciliação entre o método de observação pelo esfregaço de sangue de ponta de orelha considerado padrão ouro pela literatura, com esfregaço sanguíneo (k= 0,18) e com a capa leucoplaquetária (k = -0,12). A sensibilidade e especificidade do esfregaço sanguíneo foi de 40% e 100% e capa leucoplaquetária obteve 25% e 62%, respectivamente. O esfregaço sanguíneo com sangue obtido da jugular, mostrou-se o método com melhores resultados, quando comparado às demais técnicas, para o diagnóstico de babesiose em bovinos.Research, Society and Development2019-08-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/138810.33448/rsd-v8i10.1388Research, Society and Development; Vol. 8 No. 10; e328101388Research, Society and Development; Vol. 8 Núm. 10; e328101388Research, Society and Development; v. 8 n. 10; e3281013882525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/1388/1113Copyright (c) 2019 Lorena Silveira de Almeida, Larissa Marchiori Sena, Graziela Barioni, Théo Matos Arantes Moraes, Ronaldo Eugênio de Oliveirainfo:eu-repo/semantics/openAccessAlmeida, Lorena Silveira deSena, Larissa MarchioriBarioni, GrazielaMoraes, Théo Matos ArantesOliveira, Ronaldo Eugênio de2020-03-25T16:06:52Zoai:ojs.pkp.sfu.ca:article/1388Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:26:26.534354Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Comparison among direct evaluation methods of babesiosis diagnosis in cattle
Comparación entre los métodos de evaluación directa para el diagnóstico de babesiosis en bovinos
Comparação entre métodos de avaliação direta para o diagnóstico de babesiose em bovinos
title Comparison among direct evaluation methods of babesiosis diagnosis in cattle
spellingShingle Comparison among direct evaluation methods of babesiosis diagnosis in cattle
Almeida, Lorena Silveira de
Hemoparasitas
Esfregaço sanguíneo
Tristeza Parasitária Bovina.
Hemoparasitas
Frotis sanguíneo
Tristeza Parasitaria Bovina.
Hemoparasites
Blood smear
Bovine Parasitic Sadness.
title_short Comparison among direct evaluation methods of babesiosis diagnosis in cattle
title_full Comparison among direct evaluation methods of babesiosis diagnosis in cattle
title_fullStr Comparison among direct evaluation methods of babesiosis diagnosis in cattle
title_full_unstemmed Comparison among direct evaluation methods of babesiosis diagnosis in cattle
title_sort Comparison among direct evaluation methods of babesiosis diagnosis in cattle
author Almeida, Lorena Silveira de
author_facet Almeida, Lorena Silveira de
Sena, Larissa Marchiori
Barioni, Graziela
Moraes, Théo Matos Arantes
Oliveira, Ronaldo Eugênio de
author_role author
author2 Sena, Larissa Marchiori
Barioni, Graziela
Moraes, Théo Matos Arantes
Oliveira, Ronaldo Eugênio de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Almeida, Lorena Silveira de
Sena, Larissa Marchiori
Barioni, Graziela
Moraes, Théo Matos Arantes
Oliveira, Ronaldo Eugênio de
dc.subject.por.fl_str_mv Hemoparasitas
Esfregaço sanguíneo
Tristeza Parasitária Bovina.
Hemoparasitas
Frotis sanguíneo
Tristeza Parasitaria Bovina.
Hemoparasites
Blood smear
Bovine Parasitic Sadness.
topic Hemoparasitas
Esfregaço sanguíneo
Tristeza Parasitária Bovina.
Hemoparasitas
Frotis sanguíneo
Tristeza Parasitaria Bovina.
Hemoparasites
Blood smear
Bovine Parasitic Sadness.
description The objective of the present study was to compare three methods of direct observation: blood smear, ear tip smear and leucoplatelet cover, searching for the best method for the diagnosis of Babesia spp. in cattle. Seventy-seven bovines with suspected babesiosis were used. From these animals, blood was collected in jugular vein and ear tip. After obtaining the samples, the blood smears and the leucoplaquetary layer were made with material from the jugular and smear of the ear tip with material from the perforation of the ear tip. Of the 77 animals, 14,30% (n = 11) inclusions were found in at least one of the three evaluated methods. Of the positive animals, 36.35% (n = 4) were positive only in the blood smear and the ear tip smear alone was not able to diagnose the disease in any of the animals. The concordance coefficient obtained in the kappa test showed a poor reconciliation between the blood smear observation method, considered gold standard in the literature, with blood smear (k = 0.18) and with the leucoplatelet layer (k = -0, 12). The sensitivity and specificity of the blood smear was 40% and 100% and the leucoplatelet layer obtained 25% and 62%, respectively. The blood smear with blood obtained from the jugular was the method with the best results when compared to the other techniques for the diagnosis of babesiosis in cattle.
publishDate 2019
dc.date.none.fl_str_mv 2019-08-23
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/1388
10.33448/rsd-v8i10.1388
url https://rsdjournal.org/index.php/rsd/article/view/1388
identifier_str_mv 10.33448/rsd-v8i10.1388
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/1388/1113
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 8 No. 10; e328101388
Research, Society and Development; Vol. 8 Núm. 10; e328101388
Research, Society and Development; v. 8 n. 10; e328101388
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052776724824064