Antimicrobial activity of the extract, fractions and punicalagin from the rind of the fruit of Punica granatum against clinical isolates from cows with mastitis
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/23935 |
Resumo: | The aim of this study was to evaluate the antimicrobial activity of the extract, fractions and purified punicalagin from peel pomegranate fruit of the granada and wonderful varieties against Coagulase Positive Staphylococcus (CPS) and Coagulase- negative Staphylococcus (CNS), isolated from cows with mastitis. The pomegranate pods were dried in an oven, ground and the ethanol extract was prepared by maceration and percolation. The fractions were obtained by liquid-liquid fractionation, and the purification of punicalagin was carried out in a chromatographic column filled with Diaion® HP-20. HPLC and NMR (1H and 13C -Acetone-d6) were used for identification, quantification and structural elucidation of punicalagin and MIC (CLSI-M7A10) were determined for 23 clinical isolates. The punicalagin content was higher in the extract and fractions of the granada variety, reaching 81.5% in the purified punicalagin sample, which showed good antimicrobial activity against clinical isolates, with emphasis on S. aureus and S. schleiferi schleiferi, where the MIC was 31.75 µg/ml. Thus. punicalagin was defined as an important metabolite for the antimicrobial potential of P. granatum fruits, however, the synergism of metabolites of ethyl acetate fractions and aqueous fraction of the granada variety, had considerable importance for the good antimicrobial activity of these fractions, compared to 100 % of the CPS and CNS. Thus, the results obtained confirm the antimicrobial activity of the metabolites present in the pomegranate peel, which may support new research on pharmaceutical formulations based on P. granatum, as an alternative for the treatment, prevention and control of mastitis. |
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Antimicrobial activity of the extract, fractions and punicalagin from the rind of the fruit of Punica granatum against clinical isolates from cows with mastitisActividad antimicrobiana del extracto, fracciones y punicalagina de la cáscara del fruto de Punica granatum frente a aislados clínicos de vacas con mastitesAtividade antimicrobiana do extrato, frações e punicalagina da casca do fruto de Punica granatum frente a isolados clínicos de vacas com mastite RomãVariedade granadaVariedade wonderfulConcentração Inibitória Mínima (CIM)Cromatografia Líquida de Alta Eficiência (CLAE). PomegranateGranada varietiesWonderful varietiesMinimum Inhibitory Concentration (MIC)High Performance Liquid Chromatography (HPLC).GranadaVariedade granateVariedade wonderfulConcentración Mínima Inhibitoria (CMI)Cromatografia Líquida de Alta Resolución (CLAR).The aim of this study was to evaluate the antimicrobial activity of the extract, fractions and purified punicalagin from peel pomegranate fruit of the granada and wonderful varieties against Coagulase Positive Staphylococcus (CPS) and Coagulase- negative Staphylococcus (CNS), isolated from cows with mastitis. The pomegranate pods were dried in an oven, ground and the ethanol extract was prepared by maceration and percolation. The fractions were obtained by liquid-liquid fractionation, and the purification of punicalagin was carried out in a chromatographic column filled with Diaion® HP-20. HPLC and NMR (1H and 13C -Acetone-d6) were used for identification, quantification and structural elucidation of punicalagin and MIC (CLSI-M7A10) were determined for 23 clinical isolates. The punicalagin content was higher in the extract and fractions of the granada variety, reaching 81.5% in the purified punicalagin sample, which showed good antimicrobial activity against clinical isolates, with emphasis on S. aureus and S. schleiferi schleiferi, where the MIC was 31.75 µg/ml. Thus. punicalagin was defined as an important metabolite for the antimicrobial potential of P. granatum fruits, however, the synergism of metabolites of ethyl acetate fractions and aqueous fraction of the granada variety, had considerable importance for the good antimicrobial activity of these fractions, compared to 100 % of the CPS and CNS. Thus, the results obtained confirm the antimicrobial activity of the metabolites present in the pomegranate peel, which may support new research on pharmaceutical formulations based on P. granatum, as an alternative for the treatment, prevention and control of mastitis.El objetivo de este estudio fue evaluar la actividad antimicrobiana del extracto, fracciones y punicalagina purificada de cáscara de granada del granate y wonderful variedades contra Staphylococcus Coagulase Positive (SCP) y Staphylococcus Coagulase Negative (SCN), aisladas de vacas con mastitis. Las vainas de granada se secaron en un horno, se molieron y el extracto de etanol se preparó mediante maceración y percolación. Las fracciones se obtuvieron mediante fraccionamiento líquido-líquido y la purificación de punicalagina se realizó en una columna cromatográfica llena de Diaion® HP-20. Se utilizaron HPLC y RMN (1H y 13C -Acetona-d6) para la identificación, cuantificación y elucidación estructural de punicalagina y se determinó MIC (CLSI-M7A10) para 23 aislamientos clínicos. El contenido de punicalagina fue mayor en el extracto y fracciones de la variedad granate, alcanzando el 81,5% en la muestra de punicalagina purificada, que mostró buena actividad antimicrobiana frente a aislados clínicos, con énfasis en S. aureus y S. schleiferi schleiferi, donde la CIM fue de 31,75. µg / ml. De esta forma. La punicalagina se definió como un metabolito importante para el potencial antimicrobiano de los frutos de P. granatum, sin embargo, el sinergismo de los metabolitos de las fracciones de acetato de etilo y la fracción acuosa de la variedad granate, tuvo una importancia considerable para la buena actividad antimicrobiana de estas fracciones, en comparación con 100 % del SCP y SCN. Así, los resultados obtenidos confirman la actividad antimicrobiana de los metabolitos presentes en la cáscara de la granada, lo que puede sustentar nuevas investigaciones sobre formulaciones farmacéuticas basadas en P. granatum, como alternativa para el tratamiento, prevención y control de la mastitis.O objetivo deste estudo foi avaliar a atividade antimicrobiana do extrato, frações e punicalagina purificada da casca do fruto da romã, das variedades granada e wonderful, frente a Staphylococcus Coagulase Positivo (SCP) e Staphylococcus Coagulase Negativa (SCN), isoladas de vacas com mastite. As cascas dos frutos de romã foram secas em estufa, moídas e o extrato etanólico foi preparado por maceração e percolação. As frações foram obtidas por fracionamento líquido-líquido, e a purificação da punicalagina realizada em coluna cromatográfica preenchida com Diaion® HP-20. CLAE e RMN (1H e 13C -Acetona-d6) foram utilizados para identificação, quantificação e elucidação estrutural da punicalagina e a CIM (CLSI-M7A10) foi determinada para 23 isolados clínicos. O teor de punicalagina foi maior no extrato e frações da variedade granada, chegando a 81,5% na amostra de punicalagina purificada, que apresentou boa atividade antimicrobiana frente aos isolados clínicos, com ênfase para S. aureus e S. schleiferi schleiferi, onde a CIM foi de 31,75 μg/mL. Desta forma. a punicalagina foi definida como importante metabólito para o potencial antimicrobiano dos frutos de P. granatum contudo, o sinergismo dos metabolitos das frações acetato de etila e fração aquosa da variedade granada, teve considerável importância para a boa atividade antimicrobiana dessas frações, frente a 100% dos SCP e SCN. Assim, os resultados obtidos confirmam a atividade antimicrobiana dos metabólitos presentes na casca da romã, podendo fundamentar novas pesquisas de formulações farmacêuticas baseadas em P. granatum, como alternativa para o tratamento, prevenção e controle da mastite.Research, Society and Development2021-12-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2393510.33448/rsd-v10i16.23935Research, Society and Development; Vol. 10 No. 16; e531101623935Research, Society and Development; Vol. 10 Núm. 16; e531101623935Research, Society and Development; v. 10 n. 16; e5311016239352525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/23935/21181Copyright (c) 2021 Emannuel Ítalo Alves Campos; Liliane de Sousa Silva; Sandra Alves de Sousa Garcia; Pollyana Guimarães de Oliveira ; Milton Adriano Pelli de Oliveira ; Carla Afonso da Silva; José Realino de Paulahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCampos, Emannuel Ítalo AlvesSilva, Liliane de Sousa Garcia, Sandra Alves de SousaOliveira , Pollyana Guimarães deOliveira , Milton Adriano Pelli deSilva, Carla Afonso daPaula, José Realino de2021-12-20T11:03:07Zoai:ojs.pkp.sfu.ca:article/23935Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:42:39.194341Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Antimicrobial activity of the extract, fractions and punicalagin from the rind of the fruit of Punica granatum against clinical isolates from cows with mastitis Actividad antimicrobiana del extracto, fracciones y punicalagina de la cáscara del fruto de Punica granatum frente a aislados clínicos de vacas con mastites Atividade antimicrobiana do extrato, frações e punicalagina da casca do fruto de Punica granatum frente a isolados clínicos de vacas com mastite |
title |
Antimicrobial activity of the extract, fractions and punicalagin from the rind of the fruit of Punica granatum against clinical isolates from cows with mastitis |
spellingShingle |
Antimicrobial activity of the extract, fractions and punicalagin from the rind of the fruit of Punica granatum against clinical isolates from cows with mastitis Campos, Emannuel Ítalo Alves Romã Variedade granada Variedade wonderful Concentração Inibitória Mínima (CIM) Cromatografia Líquida de Alta Eficiência (CLAE). Pomegranate Granada varieties Wonderful varieties Minimum Inhibitory Concentration (MIC) High Performance Liquid Chromatography (HPLC). Granada Variedade granate Variedade wonderful Concentración Mínima Inhibitoria (CMI) Cromatografia Líquida de Alta Resolución (CLAR). |
title_short |
Antimicrobial activity of the extract, fractions and punicalagin from the rind of the fruit of Punica granatum against clinical isolates from cows with mastitis |
title_full |
Antimicrobial activity of the extract, fractions and punicalagin from the rind of the fruit of Punica granatum against clinical isolates from cows with mastitis |
title_fullStr |
Antimicrobial activity of the extract, fractions and punicalagin from the rind of the fruit of Punica granatum against clinical isolates from cows with mastitis |
title_full_unstemmed |
Antimicrobial activity of the extract, fractions and punicalagin from the rind of the fruit of Punica granatum against clinical isolates from cows with mastitis |
title_sort |
Antimicrobial activity of the extract, fractions and punicalagin from the rind of the fruit of Punica granatum against clinical isolates from cows with mastitis |
author |
Campos, Emannuel Ítalo Alves |
author_facet |
Campos, Emannuel Ítalo Alves Silva, Liliane de Sousa Garcia, Sandra Alves de Sousa Oliveira , Pollyana Guimarães de Oliveira , Milton Adriano Pelli de Silva, Carla Afonso da Paula, José Realino de |
author_role |
author |
author2 |
Silva, Liliane de Sousa Garcia, Sandra Alves de Sousa Oliveira , Pollyana Guimarães de Oliveira , Milton Adriano Pelli de Silva, Carla Afonso da Paula, José Realino de |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Campos, Emannuel Ítalo Alves Silva, Liliane de Sousa Garcia, Sandra Alves de Sousa Oliveira , Pollyana Guimarães de Oliveira , Milton Adriano Pelli de Silva, Carla Afonso da Paula, José Realino de |
dc.subject.por.fl_str_mv |
Romã Variedade granada Variedade wonderful Concentração Inibitória Mínima (CIM) Cromatografia Líquida de Alta Eficiência (CLAE). Pomegranate Granada varieties Wonderful varieties Minimum Inhibitory Concentration (MIC) High Performance Liquid Chromatography (HPLC). Granada Variedade granate Variedade wonderful Concentración Mínima Inhibitoria (CMI) Cromatografia Líquida de Alta Resolución (CLAR). |
topic |
Romã Variedade granada Variedade wonderful Concentração Inibitória Mínima (CIM) Cromatografia Líquida de Alta Eficiência (CLAE). Pomegranate Granada varieties Wonderful varieties Minimum Inhibitory Concentration (MIC) High Performance Liquid Chromatography (HPLC). Granada Variedade granate Variedade wonderful Concentración Mínima Inhibitoria (CMI) Cromatografia Líquida de Alta Resolución (CLAR). |
description |
The aim of this study was to evaluate the antimicrobial activity of the extract, fractions and purified punicalagin from peel pomegranate fruit of the granada and wonderful varieties against Coagulase Positive Staphylococcus (CPS) and Coagulase- negative Staphylococcus (CNS), isolated from cows with mastitis. The pomegranate pods were dried in an oven, ground and the ethanol extract was prepared by maceration and percolation. The fractions were obtained by liquid-liquid fractionation, and the purification of punicalagin was carried out in a chromatographic column filled with Diaion® HP-20. HPLC and NMR (1H and 13C -Acetone-d6) were used for identification, quantification and structural elucidation of punicalagin and MIC (CLSI-M7A10) were determined for 23 clinical isolates. The punicalagin content was higher in the extract and fractions of the granada variety, reaching 81.5% in the purified punicalagin sample, which showed good antimicrobial activity against clinical isolates, with emphasis on S. aureus and S. schleiferi schleiferi, where the MIC was 31.75 µg/ml. Thus. punicalagin was defined as an important metabolite for the antimicrobial potential of P. granatum fruits, however, the synergism of metabolites of ethyl acetate fractions and aqueous fraction of the granada variety, had considerable importance for the good antimicrobial activity of these fractions, compared to 100 % of the CPS and CNS. Thus, the results obtained confirm the antimicrobial activity of the metabolites present in the pomegranate peel, which may support new research on pharmaceutical formulations based on P. granatum, as an alternative for the treatment, prevention and control of mastitis. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-18 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/23935 10.33448/rsd-v10i16.23935 |
url |
https://rsdjournal.org/index.php/rsd/article/view/23935 |
identifier_str_mv |
10.33448/rsd-v10i16.23935 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/23935/21181 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 16; e531101623935 Research, Society and Development; Vol. 10 Núm. 16; e531101623935 Research, Society and Development; v. 10 n. 16; e531101623935 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052810173349888 |