Effect of trolox added to freezing extenders over goat and ram spermatozoa
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/14764 |
Resumo: | Semen cryopreservation is responsible for decrease the gamete fertility, due to structural and functional damages. Among the various causes, oxidative stress, resulting from the higher generation of reactive oxygen species (ROS), has been attributed to affect semen quality. Thus, it was objectified evaluate the effect of Trolox on ram and goat sperm, subjected to freezing. Semen pools of goat (n=5) and ram (n=6) were diluted in skimmed milk (7% glycerol) or Tris-egg yolk (5% glycerol) extender, respectively, added or not of Trolox (0, 20 or 40 μM/ml) and frozen. After thawing (37 °C/30 s), aliquots of semen were evaluated for lipid peroxidation by high performance liquid chromatography, coupled with a photodiode array detector (HPLC-DAD), and flow cytometry (C11-BODIPY581/591), besides of plasma membrane and acrosome integrity by fluorescence microscopy, and sperm kinetics by computerized sperm analysis (CASA). The antioxidant treatment with Trolox did not determine significant effects (p>0.05) on lipid peroxidation, plasma membrane integrity, acrosomal integrity and on the kinetic parameters evaluated. Thus, it is concluded that Trolox (20 or 40 μM) did not have a protective or deleterious effect on goats and ram sperm, submitted to freezing. |
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Effect of trolox added to freezing extenders over goat and ram spermatozoaEfecto de la adición de trolox en los diluyentes de congelación en el semen de cabra y ovejaEfeito da adição de trolox em diluentes de congelação sobre o sêmen caprino e ovinoAntioxidantesLipoperoxidaçãoRuminanteSêmenVitamina E.AntioxidantesLipoperoxidaciónRumiantesSemenVitamina E.AntioxidantsLipoperoxidationRuminantSemenVitamin E.Semen cryopreservation is responsible for decrease the gamete fertility, due to structural and functional damages. Among the various causes, oxidative stress, resulting from the higher generation of reactive oxygen species (ROS), has been attributed to affect semen quality. Thus, it was objectified evaluate the effect of Trolox on ram and goat sperm, subjected to freezing. Semen pools of goat (n=5) and ram (n=6) were diluted in skimmed milk (7% glycerol) or Tris-egg yolk (5% glycerol) extender, respectively, added or not of Trolox (0, 20 or 40 μM/ml) and frozen. After thawing (37 °C/30 s), aliquots of semen were evaluated for lipid peroxidation by high performance liquid chromatography, coupled with a photodiode array detector (HPLC-DAD), and flow cytometry (C11-BODIPY581/591), besides of plasma membrane and acrosome integrity by fluorescence microscopy, and sperm kinetics by computerized sperm analysis (CASA). The antioxidant treatment with Trolox did not determine significant effects (p>0.05) on lipid peroxidation, plasma membrane integrity, acrosomal integrity and on the kinetic parameters evaluated. Thus, it is concluded that Trolox (20 or 40 μM) did not have a protective or deleterious effect on goats and ram sperm, submitted to freezing.El proceso de criopreservación del semen provoca una disminución de la fertilidad de los gametos debido a daños estructurales y funcionales. Entre las diversas causas, el estrés oxidativo, resultante de la mayor generación de especies reactivas de oxígeno (ROS), ha sido atribuido por afectar a la calidad seminal. Así, se pretendía evaluar el efecto de Trolox en los espermatozoides de cabra y oveja sometidos a congelación. Se diluyeron pooles de semen de cabras (n=5) y ovejas (n=6) en diluyentes a base de leche desnatada (Glicerol 7%) o de Tris-gema de huevo (Glicerol 5%), respectivamente, añadidos o no de Trolox (0, 20 o 40 µM/mL), y sometido a congelación. Tras la descongelación (37 °C/30 s), se evaluó la peroxidación lipídica de las alícuotas, mediante cromatografía líquida de alto rendimiento acoplada a un detector de matriz de diodos (HPLC-DAD) y también mediante citometría de flujo (C11-BODIPY581/591), la integridad de las membranas plasmáticas y acrosómicas, por médio de microscopía de fluorescencia, y la cinética de los espermatozoides, mediante el sistema computarizado de análisis de esperma (CASA). El tratamiento antioxidante con Trolox no determinó efectos significativos (p>0,05) sobre la peroxidación lipídica, la integridad de las membranas plasmáticas y acrosomales y los parámetros cinéticos evaluados. Así pues, se concluye que Trolox (20 o 40 μM) no presenta un efecto protector o perjudicial sobre los espermatozoides de cabra y oveja sometidos a congelación.O processo de criopreservação do sêmen ocasiona a diminuição da fertilidade dos gametas, devido a danos estruturais e funcionais. Entre as várias causas, o estresse oxidativo, resultante da maior geração de espécies reativas de oxigênio (ROS), tem sido atribuído por afetar a qualidade seminal. Assim, objetivou-se avaliar o efeito do Trolox sobre os espermatozoides caprinos e ovinos submetidos à congelação. Pools de sêmen caprino (n=5) e ovino (n=6) foram diluídos em diluidores a base de leite desnatado (Glicerol 7%) ou Tris-gema de ovo (Glicerol 5%), respectivamente, adicionados ou não de Trolox (0, 20 ou 40 µM/mL), e congelados. Após descongelação (37 °C/30 s), alíquotas foram avaliadas quanto à peroxidação lipídica, por cromatografia líquida de alta performance acoplada a detector de arranjo de diodos (HPLC-DAD) e por citometria de fluxo (C11-BODIPY581/591), à integridade de membrana plasmática e acrossomal, por microscopia fluorescente, e à cinética espermática, pelo sistema computadorizado de análise espermáticas (CASA). O tratamento antioxidante com Trolox não determinou efeitos significativos (p>0,05) sobre a peroxidação lipídica, integridade de membranas plasmática e acrossomal e parâmetros cinéticos avaliados. Assim, conclui-se que o Trolox (20 ou 40 μM) não apresenta efeito protetor ou nocivo sobre os espermatozoides caprinos e ovinos submetidos à congelação.Research, Society and Development2021-04-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1476410.33448/rsd-v10i5.14764Research, Society and Development; Vol. 10 No. 5; e10310514764Research, Society and Development; Vol. 10 Núm. 5; e10310514764Research, Society and Development; v. 10 n. 5; e103105147642525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/14764/13199Copyright (c) 2021 Lúcia Cristina Pereira Arruda; Girliane Regina da Silva; André Mariano Batista; Ellen Cordeiro Bento da Silva; Helder Melo de Souza; Giovanna Isabella de Souza Couto; Tânia Maria Sarmento da Silva; Maria Madalena Pessoa Guerrahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessArruda, Lúcia Cristina PereiraSilva, Girliane Regina daBatista, André MarianoSilva, Ellen Cordeiro Bento da Souza, Helder Melo deCouto, Giovanna Isabella de SouzaSilva, Tânia Maria Sarmento daGuerra, Maria Madalena Pessoa2021-05-17T18:20:49Zoai:ojs.pkp.sfu.ca:article/14764Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:35:47.258689Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Effect of trolox added to freezing extenders over goat and ram spermatozoa Efecto de la adición de trolox en los diluyentes de congelación en el semen de cabra y oveja Efeito da adição de trolox em diluentes de congelação sobre o sêmen caprino e ovino |
| title |
Effect of trolox added to freezing extenders over goat and ram spermatozoa |
| spellingShingle |
Effect of trolox added to freezing extenders over goat and ram spermatozoa Arruda, Lúcia Cristina Pereira Antioxidantes Lipoperoxidação Ruminante Sêmen Vitamina E. Antioxidantes Lipoperoxidación Rumiantes Semen Vitamina E. Antioxidants Lipoperoxidation Ruminant Semen Vitamin E. |
| title_short |
Effect of trolox added to freezing extenders over goat and ram spermatozoa |
| title_full |
Effect of trolox added to freezing extenders over goat and ram spermatozoa |
| title_fullStr |
Effect of trolox added to freezing extenders over goat and ram spermatozoa |
| title_full_unstemmed |
Effect of trolox added to freezing extenders over goat and ram spermatozoa |
| title_sort |
Effect of trolox added to freezing extenders over goat and ram spermatozoa |
| author |
Arruda, Lúcia Cristina Pereira |
| author_facet |
Arruda, Lúcia Cristina Pereira Silva, Girliane Regina da Batista, André Mariano Silva, Ellen Cordeiro Bento da Souza, Helder Melo de Couto, Giovanna Isabella de Souza Silva, Tânia Maria Sarmento da Guerra, Maria Madalena Pessoa |
| author_role |
author |
| author2 |
Silva, Girliane Regina da Batista, André Mariano Silva, Ellen Cordeiro Bento da Souza, Helder Melo de Couto, Giovanna Isabella de Souza Silva, Tânia Maria Sarmento da Guerra, Maria Madalena Pessoa |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Arruda, Lúcia Cristina Pereira Silva, Girliane Regina da Batista, André Mariano Silva, Ellen Cordeiro Bento da Souza, Helder Melo de Couto, Giovanna Isabella de Souza Silva, Tânia Maria Sarmento da Guerra, Maria Madalena Pessoa |
| dc.subject.por.fl_str_mv |
Antioxidantes Lipoperoxidação Ruminante Sêmen Vitamina E. Antioxidantes Lipoperoxidación Rumiantes Semen Vitamina E. Antioxidants Lipoperoxidation Ruminant Semen Vitamin E. |
| topic |
Antioxidantes Lipoperoxidação Ruminante Sêmen Vitamina E. Antioxidantes Lipoperoxidación Rumiantes Semen Vitamina E. Antioxidants Lipoperoxidation Ruminant Semen Vitamin E. |
| description |
Semen cryopreservation is responsible for decrease the gamete fertility, due to structural and functional damages. Among the various causes, oxidative stress, resulting from the higher generation of reactive oxygen species (ROS), has been attributed to affect semen quality. Thus, it was objectified evaluate the effect of Trolox on ram and goat sperm, subjected to freezing. Semen pools of goat (n=5) and ram (n=6) were diluted in skimmed milk (7% glycerol) or Tris-egg yolk (5% glycerol) extender, respectively, added or not of Trolox (0, 20 or 40 μM/ml) and frozen. After thawing (37 °C/30 s), aliquots of semen were evaluated for lipid peroxidation by high performance liquid chromatography, coupled with a photodiode array detector (HPLC-DAD), and flow cytometry (C11-BODIPY581/591), besides of plasma membrane and acrosome integrity by fluorescence microscopy, and sperm kinetics by computerized sperm analysis (CASA). The antioxidant treatment with Trolox did not determine significant effects (p>0.05) on lipid peroxidation, plasma membrane integrity, acrosomal integrity and on the kinetic parameters evaluated. Thus, it is concluded that Trolox (20 or 40 μM) did not have a protective or deleterious effect on goats and ram sperm, submitted to freezing. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-04-29 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
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publishedVersion |
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https://rsdjournal.org/index.php/rsd/article/view/14764 10.33448/rsd-v10i5.14764 |
| url |
https://rsdjournal.org/index.php/rsd/article/view/14764 |
| identifier_str_mv |
10.33448/rsd-v10i5.14764 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/14764/13199 |
| dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Research, Society and Development |
| publisher.none.fl_str_mv |
Research, Society and Development |
| dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 5; e10310514764 Research, Society and Development; Vol. 10 Núm. 5; e10310514764 Research, Society and Development; v. 10 n. 5; e10310514764 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Universidade Federal de Itajubá (UNIFEI) |
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UNIFEI |
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UNIFEI |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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rsd.articles@gmail.com |
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1797052675995467776 |