In vitro production and gene expression of IGF-I and IGF-II in embryos from Nelore females submitted to administrations of recombinant bovine somatotropin

Detalhes bibliográficos
Autor(a) principal: Anjos, Rafael Soares dos
Data de Publicação: 2021
Outros Autores: Tavares , Kaio César Simiano, Martins , Leonardo Tondello, Aguiar, Luís Henrique de, Bertolini , Marcelo, Oliveira Filho, Emanuel Felipe de, Carneiro, Gustavo Ferrer
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/13263
Resumo: The present work aimed of studying the influence of recombinant bovine somatotropin (rbST) on quantity and quality of in vivo aspirated oocytes, embryo production and gene expression of insulin-like growth factors (IGFs) types I and II in in vitro produced embryos from bovine females of Nelore breed. Five cows were treated with two administrations of 2mL of saline solution as placebo, with an interval of 14 days between them, being the first administration held 19 and the second 5 days before follicular aspirations; within 30 days after these aspirations, in a crossover design, the same protocol was used in these five animals being, in this case, the saline solution replaced by 500mg of rbST in order to perform comparisons between treatments. From retrieved oocytes, in vitro production (IVP) was performed, being all stages involving this process evaluated with subsequent achievement of polymerase chain reactions in real time (qPCRs) for gene expression of IGF-I and IGF-II in produced blastocysts. It was observed that the rbST, although having a numerical increase, did not show significant influence, on the parameters studied, on the quantity and quality of in vivo aspirated oocytes, embryo production and gene expression of IGF-I and IGF-II in in vitro produced embryos from bovine females of Nelore breed.
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spelling In vitro production and gene expression of IGF-I and IGF-II in embryos from Nelore females submitted to administrations of recombinant bovine somatotropinProducción in vitro y expresión génica de IGF-I e IGF-II en embriones de hembras Nelore sometidas a administraciones de somatotropina recombinante bovinaProdução in vitro e expressão gênica de IGF-I e IGF-II em embriões de fêmeas Nelore submetidas a administrações de somatotropina recombinante bovinaEmbryosIGF-IIGF-IIIVPNelorerbST.EmbriõesIGF-IIGF-IINelorePIVErbST.EmbrionesIGF-IIGF-IINelorePIVErbST.The present work aimed of studying the influence of recombinant bovine somatotropin (rbST) on quantity and quality of in vivo aspirated oocytes, embryo production and gene expression of insulin-like growth factors (IGFs) types I and II in in vitro produced embryos from bovine females of Nelore breed. Five cows were treated with two administrations of 2mL of saline solution as placebo, with an interval of 14 days between them, being the first administration held 19 and the second 5 days before follicular aspirations; within 30 days after these aspirations, in a crossover design, the same protocol was used in these five animals being, in this case, the saline solution replaced by 500mg of rbST in order to perform comparisons between treatments. From retrieved oocytes, in vitro production (IVP) was performed, being all stages involving this process evaluated with subsequent achievement of polymerase chain reactions in real time (qPCRs) for gene expression of IGF-I and IGF-II in produced blastocysts. It was observed that the rbST, although having a numerical increase, did not show significant influence, on the parameters studied, on the quantity and quality of in vivo aspirated oocytes, embryo production and gene expression of IGF-I and IGF-II in in vitro produced embryos from bovine females of Nelore breed.Este estudio tuvo como objetivo estudiar la influencia de la somatotropina recombinante bovina (rbST) en la cantidad y calidad de los ovocitos aspirados in vivo, la producción embrionaria y la expresión génica de los factores de crecimiento insulínicos (IGFs) tipos I y II en embriones producidos in vitro a partir de Nelore hembras bovinas. Se trataron cinco vacas con dos administraciones de 2mL de solución salina como placebo, con un intervalo de catorce días entre ellas, siendo la primera administración diecinueve y la segunda cinco días antes de las aspiraciones foliculares; a los treinta días de estas aspiraciones, en un sistema cruzado, se utilizó el mismo protocolo en estos cinco animales, en cuyo caso se sustituyó la solución salina por 500mg de rbST, con el fin de realizar comparaciones entre tratamientos. A partir de los ovocitos recuperados se llevó a cabo la producción de embriones in vitro (PIVE), analizándose todas las etapas de este proceso y con la posterior realización de reacciones en cadena de la polimerasa en tiempo real (qPCRs) para IGF-I e IGF-II en los blastocistos producidos. Se observó que la rbST, aunque numéricamente influyó positivamente en los parámetros estudiados, no tuvo una influencia significativa en la cantidad y calidad de ovocitos aspirados in vivo, producción embrionaria y expresión génica de IGF-I e IGF-II en embriones producidos in vitro de hembras bovinas Nelore.Com o presente trabalho, objetivou-se estudar a influência da somatotropina recombinante bovina (rbST) sobre a quantidade e qualidade de oócitos aspirados in vivo, produção embrionária e expressão gênica dos fatores de crescimento semelhantes à insulina (IGFs) tipos I e II em embriões produzidos in vitro de fêmeas bovinas da raça Nelore. Cinco vacas foram tratadas com duas administrações de 2mL de solução salina como placebo, com intervalo de catorze dias entre elas, sendo a primeira administração realizada dezenove e a segunda cinco dias antes das aspirações foliculares; decorridos trintas dias destas aspirações, em sistema crossover, o mesmo protocolo foi utilizado nesses cinco animais sendo, neste caso, a solução salina substituída por 500mg de rbST, com o intuito de efetuar-se comparações entre os tratamentos. A partir dos oócitos recuperados, realizou-se a produção in vitro de embriões (PIVE), sendo todas as etapas que envolvem este processo analisadas e com posterior realização de reações em cadeia da polimerase em tempo real (qPCRs) para expressões gênicas de IGF-I e IGF-II nos blastocistos produzidos. Observou-se que a rbST, ainda que numericamente tenha influenciado positivamente sobre os parâmetros estudados, não exerceu influência significativa sobre a quantidade e qualidade de oócitos aspirados in vivo, produção embrionária e expressão gênica de IGF-I e IGF-II em embriões produzidos in vitro de fêmeas bovinas da raça Nelore.Research, Society and Development2021-03-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1326310.33448/rsd-v10i3.13263Research, Society and Development; Vol. 10 No. 3; e27010313263Research, Society and Development; Vol. 10 Núm. 3; e27010313263Research, Society and Development; v. 10 n. 3; e270103132632525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/13263/11976Copyright (c) 2021 Rafael Soares dos Anjos; Kaio César Simiano Tavares ; Leonardo Tondello Martins ; Luís Henrique de Aguiar; Marcelo Bertolini ; Emanuel Felipe de Oliveira Filho; Gustavo Ferrer Carneirohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAnjos, Rafael Soares dos Tavares , Kaio César Simiano Martins , Leonardo Tondello Aguiar, Luís Henrique de Bertolini , Marcelo Oliveira Filho, Emanuel Felipe de Carneiro, Gustavo Ferrer 2021-03-28T12:03:35Zoai:ojs.pkp.sfu.ca:article/13263Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:34:39.107649Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv In vitro production and gene expression of IGF-I and IGF-II in embryos from Nelore females submitted to administrations of recombinant bovine somatotropin
Producción in vitro y expresión génica de IGF-I e IGF-II en embriones de hembras Nelore sometidas a administraciones de somatotropina recombinante bovina
Produção in vitro e expressão gênica de IGF-I e IGF-II em embriões de fêmeas Nelore submetidas a administrações de somatotropina recombinante bovina
title In vitro production and gene expression of IGF-I and IGF-II in embryos from Nelore females submitted to administrations of recombinant bovine somatotropin
spellingShingle In vitro production and gene expression of IGF-I and IGF-II in embryos from Nelore females submitted to administrations of recombinant bovine somatotropin
Anjos, Rafael Soares dos
Embryos
IGF-I
IGF-II
IVP
Nelore
rbST.
Embriões
IGF-I
IGF-II
Nelore
PIVE
rbST.
Embriones
IGF-I
IGF-II
Nelore
PIVE
rbST.
title_short In vitro production and gene expression of IGF-I and IGF-II in embryos from Nelore females submitted to administrations of recombinant bovine somatotropin
title_full In vitro production and gene expression of IGF-I and IGF-II in embryos from Nelore females submitted to administrations of recombinant bovine somatotropin
title_fullStr In vitro production and gene expression of IGF-I and IGF-II in embryos from Nelore females submitted to administrations of recombinant bovine somatotropin
title_full_unstemmed In vitro production and gene expression of IGF-I and IGF-II in embryos from Nelore females submitted to administrations of recombinant bovine somatotropin
title_sort In vitro production and gene expression of IGF-I and IGF-II in embryos from Nelore females submitted to administrations of recombinant bovine somatotropin
author Anjos, Rafael Soares dos
author_facet Anjos, Rafael Soares dos
Tavares , Kaio César Simiano
Martins , Leonardo Tondello
Aguiar, Luís Henrique de
Bertolini , Marcelo
Oliveira Filho, Emanuel Felipe de
Carneiro, Gustavo Ferrer
author_role author
author2 Tavares , Kaio César Simiano
Martins , Leonardo Tondello
Aguiar, Luís Henrique de
Bertolini , Marcelo
Oliveira Filho, Emanuel Felipe de
Carneiro, Gustavo Ferrer
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Anjos, Rafael Soares dos
Tavares , Kaio César Simiano
Martins , Leonardo Tondello
Aguiar, Luís Henrique de
Bertolini , Marcelo
Oliveira Filho, Emanuel Felipe de
Carneiro, Gustavo Ferrer
dc.subject.por.fl_str_mv Embryos
IGF-I
IGF-II
IVP
Nelore
rbST.
Embriões
IGF-I
IGF-II
Nelore
PIVE
rbST.
Embriones
IGF-I
IGF-II
Nelore
PIVE
rbST.
topic Embryos
IGF-I
IGF-II
IVP
Nelore
rbST.
Embriões
IGF-I
IGF-II
Nelore
PIVE
rbST.
Embriones
IGF-I
IGF-II
Nelore
PIVE
rbST.
description The present work aimed of studying the influence of recombinant bovine somatotropin (rbST) on quantity and quality of in vivo aspirated oocytes, embryo production and gene expression of insulin-like growth factors (IGFs) types I and II in in vitro produced embryos from bovine females of Nelore breed. Five cows were treated with two administrations of 2mL of saline solution as placebo, with an interval of 14 days between them, being the first administration held 19 and the second 5 days before follicular aspirations; within 30 days after these aspirations, in a crossover design, the same protocol was used in these five animals being, in this case, the saline solution replaced by 500mg of rbST in order to perform comparisons between treatments. From retrieved oocytes, in vitro production (IVP) was performed, being all stages involving this process evaluated with subsequent achievement of polymerase chain reactions in real time (qPCRs) for gene expression of IGF-I and IGF-II in produced blastocysts. It was observed that the rbST, although having a numerical increase, did not show significant influence, on the parameters studied, on the quantity and quality of in vivo aspirated oocytes, embryo production and gene expression of IGF-I and IGF-II in in vitro produced embryos from bovine females of Nelore breed.
publishDate 2021
dc.date.none.fl_str_mv 2021-03-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/13263
10.33448/rsd-v10i3.13263
url https://rsdjournal.org/index.php/rsd/article/view/13263
identifier_str_mv 10.33448/rsd-v10i3.13263
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/13263/11976
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 3; e27010313263
Research, Society and Development; Vol. 10 Núm. 3; e27010313263
Research, Society and Development; v. 10 n. 3; e27010313263
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052671888195584

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