Mode of action of potassium phosphite in the growth and development of Phytophthora nicotianae, causal agent of gummosis in citrus
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/8822 |
Resumo: | The aim of this study was to evaluate the direct effect of Phytogard®, a product based on potassium phosphite, on the development of Phytophthora nicotianae and to verify possible mode of action of this product on the pathogen. The pathogen's mycelium was exposed to increasing concentrations of Phytogard® and mycelial growth, production of fresh mycelium and zoospores production were evaluated. Regarding the possible mode of action of Phytogard® on P. nicotianae, the hypha morphology, electrolyte leakage, lipid peroxidation, protein synthesis and β-1,3-glucanase activity were evaluated. The pathogen mycelium was inhibited as the phosphite concentration was increased. The production of zoospores was reduced from the lowest concentration of Phytogard® used. There were morphological changes in the pathogen's hyphae and the electrolyte leakage increased as the concentration of the product increased and over time. There was no difference between treatments in the analysis of lipid peroxidation and total protein. There was a decrease in the activity of the enzyme β-1,3-glucanase as the concentrations of Phytogard® increased. It is concluded that Phytogard® inhibited mycelial growth, production of fresh mycelium and zoospores production of P. nicotianae. Furthermore, the product decreases the thickness of hyphae and increases the number of atrophied ramifications, in addition to harming plasma membrane permeability and cell wall synthesis of the pathogen. |
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Mode of action of potassium phosphite in the growth and development of Phytophthora nicotianae, causal agent of gummosis in citrusMecanismos de acción del fosfito de potasio en el crecimiento y desarrollo de Phytophthora nicotianae, agente causal de la gomosis en cítricosMecanismos de ação do fosfito de potássio no crescimento e desenvolvimento de Phytophthora nicotianae, agente causal da gomose em citrosMembrana plasmáticaParede celularControle alternativo.Membrana plasmáticaPared celularControl alternativo.Plasma membraneCell wallAlternative control.The aim of this study was to evaluate the direct effect of Phytogard®, a product based on potassium phosphite, on the development of Phytophthora nicotianae and to verify possible mode of action of this product on the pathogen. The pathogen's mycelium was exposed to increasing concentrations of Phytogard® and mycelial growth, production of fresh mycelium and zoospores production were evaluated. Regarding the possible mode of action of Phytogard® on P. nicotianae, the hypha morphology, electrolyte leakage, lipid peroxidation, protein synthesis and β-1,3-glucanase activity were evaluated. The pathogen mycelium was inhibited as the phosphite concentration was increased. The production of zoospores was reduced from the lowest concentration of Phytogard® used. There were morphological changes in the pathogen's hyphae and the electrolyte leakage increased as the concentration of the product increased and over time. There was no difference between treatments in the analysis of lipid peroxidation and total protein. There was a decrease in the activity of the enzyme β-1,3-glucanase as the concentrations of Phytogard® increased. It is concluded that Phytogard® inhibited mycelial growth, production of fresh mycelium and zoospores production of P. nicotianae. Furthermore, the product decreases the thickness of hyphae and increases the number of atrophied ramifications, in addition to harming plasma membrane permeability and cell wall synthesis of the pathogen.El objetivo de este estudio fue evaluar el efecto de Phytogard®, un producto a base de fosfito de potasio, sobre el desarrollo de Phytophthora nicotianae y verificar los posibles mecanismos de acción de este producto sobre el patógeno. El micelio del patógeno fue expuesto a concentraciones crecientes de Phytogard® y se evaluó el crecimiento del micelio, la producción de la masa fresca del micelio y la producción de zoosporas. En cuanto a los posibles mecanismos de acción de Phytogard® sobre P. nicotianae, se evaluaron la morfología de las hifas, la pérdida de electrolitos, la peroxidación de lípidos, la síntesis de proteínas y la actividad de la β-1,3-glucanasa. El micelio del patógeno se inhibió al aumentar la concentración de fosfito. La producción de zoosporas se redujo a partir de la menor concentración de Phytogard® utilizada. Se produjeron cambios morfológicos en las hifas del patógeno y la pérdida de electrolitos se incrementó a medida que la concentración del producto aumentaba y con el tiempo. No hubo diferencia entre tratamientos en los análisis de peroxidación de lípidos y proteínas totales. Hubo una disminución en la actividad de la enzima β-1,3-glucanasa a medida que aumentaron las concentraciones de Phytogard®. Se concluye que Phytogard® inhibe el crecimiento del micelio, la producción de la masa fresca del micelio y zoosporas de P. nicotianae. Además, el producto disminuye el grosor de las hifas y aumenta las ramificaciones atrofiadas, además de perjudicar la permeabilidad de la membrana plasmática y la síntesis de la pared celular del patógeno.O objetivo desse estudo foi avaliar o efeito direto do Phytogard®, produto à base de fosfito de potássio, sobre o desenvolvimento de Phytophthora nicotianae e verificar possíveis mecanismos de ação desse produto sobre o patógeno. O micélio do patógeno foi exposto a concentrações crescentes de Phytogard® sendo avaliado o crescimento micelial, produção de massa fresca de micélio e produção de zoósporos. Quanto aos possíveis mecanismos de ação do Phytogard® sobre P. nicotianae, avaliou-se a morfologia das hifas, perda de eletrólitos, peroxidação de lipídios, síntese de proteínas e atividade da enzima β-1,3-glucanase. O micélio do patógeno foi inibido à medida que se aumentou a concentração de fosfito. A produção de zoósporos foi reduzida a partir da menor concentração de Phytogard® utilizada. Houve mudanças morfológicas nas hifas do patógeno e a perda de eletrólitos foi crescente à medida que se aumentou a concentração do produto e ao longo do tempo. Não houve diferença entre os tratamentos nas análises de peroxidação de lipídios e proteínas totais. Houve decréscimo na atividade da enzima β-1,3-glucanase à medida que as concentrações de Phytogard® aumentaram. Conclui-se que o Phytogard® inibe o crescimento micelial, a produção de massa fresca de micélio e de zoósporos de P. nicotianae. Além disso, o produto diminui a espessura das hifas e aumenta o número de ramificações atrofiadas, além de prejudicar a permeabilidade da membrana plasmática e a síntese de parede celular do patógeno.Research, Society and Development2020-10-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/882210.33448/rsd-v9i10.8822Research, Society and Development; Vol. 9 No. 10; e5369108822Research, Society and Development; Vol. 9 Núm. 10; e5369108822Research, Society and Development; v. 9 n. 10; e53691088222525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/8822/7899Copyright (c) 2020 Dalilla Carvalho Rezende; Dayson Fernando Ribeiro Brandão; Simone Cristiane Brand ; Silvia Blumer; Sérgio Florentino Pascholati; Natália Moreira Mafrahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRezende, Dalilla Carvalho Brandão, Dayson Fernando Ribeiro Brand , Simone CristianeBlumer, SilviaPascholati, Sérgio FlorentinoMafra, Natália Moreira2020-10-31T12:03:23Zoai:ojs.pkp.sfu.ca:article/8822Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:31:12.572013Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Mode of action of potassium phosphite in the growth and development of Phytophthora nicotianae, causal agent of gummosis in citrus Mecanismos de acción del fosfito de potasio en el crecimiento y desarrollo de Phytophthora nicotianae, agente causal de la gomosis en cítricos Mecanismos de ação do fosfito de potássio no crescimento e desenvolvimento de Phytophthora nicotianae, agente causal da gomose em citros |
title |
Mode of action of potassium phosphite in the growth and development of Phytophthora nicotianae, causal agent of gummosis in citrus |
spellingShingle |
Mode of action of potassium phosphite in the growth and development of Phytophthora nicotianae, causal agent of gummosis in citrus Rezende, Dalilla Carvalho Membrana plasmática Parede celular Controle alternativo. Membrana plasmática Pared celular Control alternativo. Plasma membrane Cell wall Alternative control. |
title_short |
Mode of action of potassium phosphite in the growth and development of Phytophthora nicotianae, causal agent of gummosis in citrus |
title_full |
Mode of action of potassium phosphite in the growth and development of Phytophthora nicotianae, causal agent of gummosis in citrus |
title_fullStr |
Mode of action of potassium phosphite in the growth and development of Phytophthora nicotianae, causal agent of gummosis in citrus |
title_full_unstemmed |
Mode of action of potassium phosphite in the growth and development of Phytophthora nicotianae, causal agent of gummosis in citrus |
title_sort |
Mode of action of potassium phosphite in the growth and development of Phytophthora nicotianae, causal agent of gummosis in citrus |
author |
Rezende, Dalilla Carvalho |
author_facet |
Rezende, Dalilla Carvalho Brandão, Dayson Fernando Ribeiro Brand , Simone Cristiane Blumer, Silvia Pascholati, Sérgio Florentino Mafra, Natália Moreira |
author_role |
author |
author2 |
Brandão, Dayson Fernando Ribeiro Brand , Simone Cristiane Blumer, Silvia Pascholati, Sérgio Florentino Mafra, Natália Moreira |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Rezende, Dalilla Carvalho Brandão, Dayson Fernando Ribeiro Brand , Simone Cristiane Blumer, Silvia Pascholati, Sérgio Florentino Mafra, Natália Moreira |
dc.subject.por.fl_str_mv |
Membrana plasmática Parede celular Controle alternativo. Membrana plasmática Pared celular Control alternativo. Plasma membrane Cell wall Alternative control. |
topic |
Membrana plasmática Parede celular Controle alternativo. Membrana plasmática Pared celular Control alternativo. Plasma membrane Cell wall Alternative control. |
description |
The aim of this study was to evaluate the direct effect of Phytogard®, a product based on potassium phosphite, on the development of Phytophthora nicotianae and to verify possible mode of action of this product on the pathogen. The pathogen's mycelium was exposed to increasing concentrations of Phytogard® and mycelial growth, production of fresh mycelium and zoospores production were evaluated. Regarding the possible mode of action of Phytogard® on P. nicotianae, the hypha morphology, electrolyte leakage, lipid peroxidation, protein synthesis and β-1,3-glucanase activity were evaluated. The pathogen mycelium was inhibited as the phosphite concentration was increased. The production of zoospores was reduced from the lowest concentration of Phytogard® used. There were morphological changes in the pathogen's hyphae and the electrolyte leakage increased as the concentration of the product increased and over time. There was no difference between treatments in the analysis of lipid peroxidation and total protein. There was a decrease in the activity of the enzyme β-1,3-glucanase as the concentrations of Phytogard® increased. It is concluded that Phytogard® inhibited mycelial growth, production of fresh mycelium and zoospores production of P. nicotianae. Furthermore, the product decreases the thickness of hyphae and increases the number of atrophied ramifications, in addition to harming plasma membrane permeability and cell wall synthesis of the pathogen. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-07 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/8822 10.33448/rsd-v9i10.8822 |
url |
https://rsdjournal.org/index.php/rsd/article/view/8822 |
identifier_str_mv |
10.33448/rsd-v9i10.8822 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/8822/7899 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 9 No. 10; e5369108822 Research, Society and Development; Vol. 9 Núm. 10; e5369108822 Research, Society and Development; v. 9 n. 10; e5369108822 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052660700938240 |