Antimicrobial peptides expressed in the fat body of Spodoptera frugiperda (Lepidoptera: Noctuidae) in response to the microbial challenge

Detalhes bibliográficos
Autor(a) principal: Feistler, Elisiane Pinto
Data de Publicação: 2022
Outros Autores: Maller, Alexandre, Simão, Rita de Cássia Garcia, Kadowaki, Marina Kimiko, Alves , Luis Francisco Angeli, Silva, José Luis da Conceição
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/35263
Resumo: Spodoptera frugiperda (Lepidoptera: Noctuidae) is the key pest in maize crops and has a major impact on world agriculture. Several studies on the biological control of this species have been carried out, and these demonstrate resistance to biological control and pesticides. However, the humoral immune system of S. frugiperda is not yet completely known in relation to the innate immune response to biological control agents. The aim of this study was to evaluate the expression of Gloverin, Sf-gallerimycin, and Attacin genes in the fat body of larvae at the 6th instar of development. Our work confirmed the expression of Sf-gallerimycin in response to the bacterial challenge, 24 h post-inoculation, and revealed the expression of Gloverin and Attacin for the first time for both bacterial challenge and entomopathogenic fungus Beauveria bassiana challenge. Gloverin and Attacin genes were upregulated under the conditions analyzed. In addition, we revealed the presence of two probable antimicrobial peptides in the hemolymph, induced 24 h post challenge with the microorganisms. The 4.7 kDa band b is probably a defensin-like peptide, and the 6.1 kDa band a is a peptide not yet reported in S. frugiperda.
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spelling Antimicrobial peptides expressed in the fat body of Spodoptera frugiperda (Lepidoptera: Noctuidae) in response to the microbial challengePéptidos antimicrobianos expresados en el cuerpo graso de Spodoptera frugiperda (Lepidoptera: Noctuidae) en respuesta al desafío microbianoPeptídeos antimicrobianos expressos no corpo gorduroso de Spodoptera frugiperda (Lepidoptera: Noctuidae) em resposta ao desafio microbianoPéptidos antimicrobianosBeauveria bassianaCuerpo gordoSistema inmunitarioExpresion genica.Peptídeos antimicrobianosBeauveria bassianaCorpo gordoSistema imunológicoExpressão genética.Antimicrobial peptidesBeauveria bassianaFat bodyImmune systemGene expression.Spodoptera frugiperda (Lepidoptera: Noctuidae) is the key pest in maize crops and has a major impact on world agriculture. Several studies on the biological control of this species have been carried out, and these demonstrate resistance to biological control and pesticides. However, the humoral immune system of S. frugiperda is not yet completely known in relation to the innate immune response to biological control agents. The aim of this study was to evaluate the expression of Gloverin, Sf-gallerimycin, and Attacin genes in the fat body of larvae at the 6th instar of development. Our work confirmed the expression of Sf-gallerimycin in response to the bacterial challenge, 24 h post-inoculation, and revealed the expression of Gloverin and Attacin for the first time for both bacterial challenge and entomopathogenic fungus Beauveria bassiana challenge. Gloverin and Attacin genes were upregulated under the conditions analyzed. In addition, we revealed the presence of two probable antimicrobial peptides in the hemolymph, induced 24 h post challenge with the microorganisms. The 4.7 kDa band b is probably a defensin-like peptide, and the 6.1 kDa band a is a peptide not yet reported in S. frugiperda.Spodoptera frugiperda (Lepidoptera: Noctuidae) es la principal plaga del maíz y tiene un gran impacto en la agricultura mundial. Se han realizado varios estudios sobre el control biológico de esta especie, y estos demuestran resistencia al control biológico y plaguicidas. Sin embargo, el sistema inmune humoral de S. frugiperda aún no se comprende completamente en relación con la respuesta inmune innata a los agentes de control biológico. El objetivo de este trabajo fue evaluar la expresión de los genes Gloverina, Sf-galerimicina y Attacina en el cuerpo graso de larvas en el sexto estadio de desarrollo. Nuestro trabajo confirmó la expresión de Sf-galerimicina en respuesta al desafío bacteriano, 24 h después de la inoculación, y reveló por primera vez la expresión de Gloverina y Attacina, tanto con el desafío bacteriano como con el hongo entomopatógeno Beauveria bassiana. Los genes de Gloverina y Attacina aumentaron en las condiciones analizadas. Además, revelamos la presencia de dos probables péptidos antimicrobianos en hemolinfa, inducidos 24 h después del desafío con microorganismos. La banda b de 4,7 kDa es probablemente un péptido similar a la defensina, y la banda a de 6,1 kDa es un péptido aún no informado en S. frugiperda.Spodoptera frugiperda (Lepidoptera: Noctuidae) é a principal praga da cultura do milho e tem grande impacto na agricultura mundial. Vários estudos sobre o controle biológico desta espécie têm sido realizados, e estes demonstram resistência ao controle biológico e agrotóxicos. No entanto, o sistema imune humoral de S. frugiperda ainda não é completamente conhecido em relação à resposta imune inata para agentes de controle biológico. O objetivo deste estudo foi avaliar a expressão dos genes Gloverin, Sf-galerimycin e Attacin no corpo gorduroso de larvas no 6º instar de desenvolvimento. Nosso trabalho confirmou a expressão de Sf-galerimicina em resposta ao desafio bacteriano, 24 h após a inoculação, e revelou pela primeira vez a expressão de Gloverina e Attacina, tanto com o desafio bacteriano quanto com o fungo entomopatogênico Beauveria bassiana. Os genes Gloverina e Attacina foram regulados positivamente nas condições analisadas. Além disso, revelamos a presença de dois prováveis ​​peptídeos antimicrobianos na hemolinfa, induzidos 24 h após o desafio com os microrganismos. A banda b de 4,7 kDa é provavelmente um peptídeo do tipo defensina, e a banda a de 6,1 kDa é um peptídeo ainda não relatado em S. frugiperda.Research, Society and Development2022-09-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3526310.33448/rsd-v11i13.35263Research, Society and Development; Vol. 11 No. 13; e81111335263Research, Society and Development; Vol. 11 Núm. 13; e81111335263Research, Society and Development; v. 11 n. 13; e811113352632525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/35263/29495Copyright (c) 2022 Elisiane Pinto Feistler; Alexandre Maller; Rita de Cássia Garcia Simão; Marina Kimiko Kadowaki; Luis Francisco Angeli Alves ; José Luis da Conceição Silvahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFeistler, Elisiane Pinto Maller, AlexandreSimão, Rita de Cássia Garcia Kadowaki, Marina Kimiko Alves , Luis Francisco Angeli Silva, José Luis da Conceição 2022-10-17T13:43:46Zoai:ojs.pkp.sfu.ca:article/35263Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:50:13.997290Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Antimicrobial peptides expressed in the fat body of Spodoptera frugiperda (Lepidoptera: Noctuidae) in response to the microbial challenge
Péptidos antimicrobianos expresados en el cuerpo graso de Spodoptera frugiperda (Lepidoptera: Noctuidae) en respuesta al desafío microbiano
Peptídeos antimicrobianos expressos no corpo gorduroso de Spodoptera frugiperda (Lepidoptera: Noctuidae) em resposta ao desafio microbiano
title Antimicrobial peptides expressed in the fat body of Spodoptera frugiperda (Lepidoptera: Noctuidae) in response to the microbial challenge
spellingShingle Antimicrobial peptides expressed in the fat body of Spodoptera frugiperda (Lepidoptera: Noctuidae) in response to the microbial challenge
Feistler, Elisiane Pinto
Péptidos antimicrobianos
Beauveria bassiana
Cuerpo gordo
Sistema inmunitario
Expresion genica.
Peptídeos antimicrobianos
Beauveria bassiana
Corpo gordo
Sistema imunológico
Expressão genética.
Antimicrobial peptides
Beauveria bassiana
Fat body
Immune system
Gene expression.
title_short Antimicrobial peptides expressed in the fat body of Spodoptera frugiperda (Lepidoptera: Noctuidae) in response to the microbial challenge
title_full Antimicrobial peptides expressed in the fat body of Spodoptera frugiperda (Lepidoptera: Noctuidae) in response to the microbial challenge
title_fullStr Antimicrobial peptides expressed in the fat body of Spodoptera frugiperda (Lepidoptera: Noctuidae) in response to the microbial challenge
title_full_unstemmed Antimicrobial peptides expressed in the fat body of Spodoptera frugiperda (Lepidoptera: Noctuidae) in response to the microbial challenge
title_sort Antimicrobial peptides expressed in the fat body of Spodoptera frugiperda (Lepidoptera: Noctuidae) in response to the microbial challenge
author Feistler, Elisiane Pinto
author_facet Feistler, Elisiane Pinto
Maller, Alexandre
Simão, Rita de Cássia Garcia
Kadowaki, Marina Kimiko
Alves , Luis Francisco Angeli
Silva, José Luis da Conceição
author_role author
author2 Maller, Alexandre
Simão, Rita de Cássia Garcia
Kadowaki, Marina Kimiko
Alves , Luis Francisco Angeli
Silva, José Luis da Conceição
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Feistler, Elisiane Pinto
Maller, Alexandre
Simão, Rita de Cássia Garcia
Kadowaki, Marina Kimiko
Alves , Luis Francisco Angeli
Silva, José Luis da Conceição
dc.subject.por.fl_str_mv Péptidos antimicrobianos
Beauveria bassiana
Cuerpo gordo
Sistema inmunitario
Expresion genica.
Peptídeos antimicrobianos
Beauveria bassiana
Corpo gordo
Sistema imunológico
Expressão genética.
Antimicrobial peptides
Beauveria bassiana
Fat body
Immune system
Gene expression.
topic Péptidos antimicrobianos
Beauveria bassiana
Cuerpo gordo
Sistema inmunitario
Expresion genica.
Peptídeos antimicrobianos
Beauveria bassiana
Corpo gordo
Sistema imunológico
Expressão genética.
Antimicrobial peptides
Beauveria bassiana
Fat body
Immune system
Gene expression.
description Spodoptera frugiperda (Lepidoptera: Noctuidae) is the key pest in maize crops and has a major impact on world agriculture. Several studies on the biological control of this species have been carried out, and these demonstrate resistance to biological control and pesticides. However, the humoral immune system of S. frugiperda is not yet completely known in relation to the innate immune response to biological control agents. The aim of this study was to evaluate the expression of Gloverin, Sf-gallerimycin, and Attacin genes in the fat body of larvae at the 6th instar of development. Our work confirmed the expression of Sf-gallerimycin in response to the bacterial challenge, 24 h post-inoculation, and revealed the expression of Gloverin and Attacin for the first time for both bacterial challenge and entomopathogenic fungus Beauveria bassiana challenge. Gloverin and Attacin genes were upregulated under the conditions analyzed. In addition, we revealed the presence of two probable antimicrobial peptides in the hemolymph, induced 24 h post challenge with the microorganisms. The 4.7 kDa band b is probably a defensin-like peptide, and the 6.1 kDa band a is a peptide not yet reported in S. frugiperda.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/35263
10.33448/rsd-v11i13.35263
url https://rsdjournal.org/index.php/rsd/article/view/35263
identifier_str_mv 10.33448/rsd-v11i13.35263
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/35263/29495
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 13; e81111335263
Research, Society and Development; Vol. 11 Núm. 13; e81111335263
Research, Society and Development; v. 11 n. 13; e81111335263
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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