Optimal size of plots for experiments with in vitro establishment of species of the passiflora genus
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/9354 |
Resumo: | The genus Passiflora have great commercial, ornamental and phytotherapeutic importance. However, information on the ideal plot size in experiments aiming at the in vitro establishment of Passiflora is scarce. This study aimed at determining the optimal size of plots in experiments of in vitro establishment of the species Passiflora edulis Sims f. edulis, Passiflora giberti N. E. Brown. and Passiflora laurifolia L. For each species, uniformity tests were conducted with two concentrations of salts of the culture medium (MS and ½ MS) and for each test, evaluations were performed at 45, 75 and 105 days after incubation of the explants. In each evaluation and culture medium, different sizes of plots were simulated, ranging from one (1) basic unit to 50 per plot, with each basic unit consisting of a plant. To estimate the optimum plot size, the modified maximum curvature method was used. In in vitro establishment experiments, the recommended optimal plot size should be 11 explants for P. edulis Sims f. edulis, of 15 explants for P. giberti N. E. Brown. and 9 explants for P. laurifolia L. |
id |
UNIFEI_e51496645ef7238c3bfe6315c6da06f1 |
---|---|
oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/9354 |
network_acronym_str |
UNIFEI |
network_name_str |
Research, Society and Development |
repository_id_str |
|
spelling |
Optimal size of plots for experiments with in vitro establishment of species of the passiflora genusTamaño óptimo de las parcelas para experimentos aplicados al establecimiento in vitro de tres especies del género PassifloraTamanho ótimo de parcelas para experimentos de estabelecimento in vitro em espécies do gênero passifloraPrecisão experimentalCoeficiente de variaçãoEnsaio de uniformidade.Precisión experimentalCoeficiente de variaciónPrueba de uniformidad.Experimental precisionCoefficient of variationUniformity test.The genus Passiflora have great commercial, ornamental and phytotherapeutic importance. However, information on the ideal plot size in experiments aiming at the in vitro establishment of Passiflora is scarce. This study aimed at determining the optimal size of plots in experiments of in vitro establishment of the species Passiflora edulis Sims f. edulis, Passiflora giberti N. E. Brown. and Passiflora laurifolia L. For each species, uniformity tests were conducted with two concentrations of salts of the culture medium (MS and ½ MS) and for each test, evaluations were performed at 45, 75 and 105 days after incubation of the explants. In each evaluation and culture medium, different sizes of plots were simulated, ranging from one (1) basic unit to 50 per plot, with each basic unit consisting of a plant. To estimate the optimum plot size, the modified maximum curvature method was used. In in vitro establishment experiments, the recommended optimal plot size should be 11 explants for P. edulis Sims f. edulis, of 15 explants for P. giberti N. E. Brown. and 9 explants for P. laurifolia L.El género Passiflora tiene una gran importancia comercial, ornamental y fitoterapéutica. Sin embargo, la información sobre el tamaño de parcela ideal en los experimentos que tienen como objetivo el establecimiento in vitro de Passiflora es escasa. El objetivo de este trabajo fue determinar el tamaño óptimo de parcela en experimentos para el establecimiento in vitro de Passiflora edulis Sims f. edulis, Passiflora giberti N. E. Brown. y Passiflora laurifolia L. Para cada especie se realizaron pruebas de uniformidad con dos concentraciones de sales del medio de cultivo (MS y ½ MS) y en cada ensayo se realizaron evaluaciones a los 45, 75 y 105 días después de la incubación de los explantes. En cada medio de evaluación y cultivo se simularon diferentes tamaños de parcelas, que van desde una (1) unidad básica hasta 50 por parcela, siendo cada unidad básica una planta. Para estimar el tamaño óptimo de la parcela, se utilizó el método de curvatura máxima modificado. En experimentos de establecimiento in vitro, el tamaño de parcela óptimo recomendado debe ser de 11 explantes para P. edulis Sims f. edulis, de 15 explantes para P. giberti N. E. Brown. y 9 explantes para P. laurifolia L.As espécies do gênero Passiflora apresentam grande importância comercial, ornamental e fitoterapêutica. Contudo, são escassas as informações sobre o tamanho ideal de parcelas em experimentos visando o estabelecimento in vitro de Passiflora. Este trabalho teve por objetivo determinar o tamanho ótimo de parcelas em experimentos de estabelecimento in vitro das espécies de Passiflora edulis Sims f. edulis, Passiflora giberti N. E. Brown. e Passiflora laurifolia L. Para cada espécie foram conduzidos ensaios de uniformidade com duas concentrações de sais do meio de cultura (MS e ½ MS) e em cada ensaio realizaram-se avaliações aos 45, 75 e 105 dias após incubação dos explantes. Em cada avaliação e meio de cultura foram simulados diversos tamanhos de parcelas, variando de uma (1) unidade básica até 50 por parcela, sendo cada unidade básica constituída por uma planta. Para a estimação do tamanho ótimo de parcelas empregou-se o método da máxima curvatura modificado. Em experimentos de estabelecimento in vitro o tamanho ótimo de parcela recomendado deve ser o de 11 explantes para P. edulis Sims f. edulis, de 15 explantes para P. giberti N. E. Brown. e de 9 explantes para P. laurifolia L..Research, Society and Development2020-10-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/935410.33448/rsd-v9i10.9354Research, Society and Development; Vol. 9 No. 10; e8859109354Research, Society and Development; Vol. 9 Núm. 10; e8859109354Research, Society and Development; v. 9 n. 10; e88591093542525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/9354/8327Copyright (c) 2020 Glaucia Amorim Faria; Ana Patricia Bastos Peixoto; Augusto Ramalho de Morais; Táis Ferreira Costa; Cintia Patrícia Martins de Oliveira; Beatriz Garcia Lopes; Paula Suares Rocha; Tiago Almeida de Oliveira; Lucas Menezes Felizardohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFaria, Glaucia Amorim Peixoto, Ana Patricia Bastos Morais, Augusto Ramalho de Costa, Táis Ferreira Oliveira, Cintia Patrícia Martins de Lopes, Beatriz Garcia Rocha, Paula Suares Oliveira, Tiago Almeida de Felizardo, Lucas Menezes 2020-10-31T12:03:23Zoai:ojs.pkp.sfu.ca:article/9354Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:31:39.778476Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Optimal size of plots for experiments with in vitro establishment of species of the passiflora genus Tamaño óptimo de las parcelas para experimentos aplicados al establecimiento in vitro de tres especies del género Passiflora Tamanho ótimo de parcelas para experimentos de estabelecimento in vitro em espécies do gênero passiflora |
title |
Optimal size of plots for experiments with in vitro establishment of species of the passiflora genus |
spellingShingle |
Optimal size of plots for experiments with in vitro establishment of species of the passiflora genus Faria, Glaucia Amorim Precisão experimental Coeficiente de variação Ensaio de uniformidade. Precisión experimental Coeficiente de variación Prueba de uniformidad. Experimental precision Coefficient of variation Uniformity test. |
title_short |
Optimal size of plots for experiments with in vitro establishment of species of the passiflora genus |
title_full |
Optimal size of plots for experiments with in vitro establishment of species of the passiflora genus |
title_fullStr |
Optimal size of plots for experiments with in vitro establishment of species of the passiflora genus |
title_full_unstemmed |
Optimal size of plots for experiments with in vitro establishment of species of the passiflora genus |
title_sort |
Optimal size of plots for experiments with in vitro establishment of species of the passiflora genus |
author |
Faria, Glaucia Amorim |
author_facet |
Faria, Glaucia Amorim Peixoto, Ana Patricia Bastos Morais, Augusto Ramalho de Costa, Táis Ferreira Oliveira, Cintia Patrícia Martins de Lopes, Beatriz Garcia Rocha, Paula Suares Oliveira, Tiago Almeida de Felizardo, Lucas Menezes |
author_role |
author |
author2 |
Peixoto, Ana Patricia Bastos Morais, Augusto Ramalho de Costa, Táis Ferreira Oliveira, Cintia Patrícia Martins de Lopes, Beatriz Garcia Rocha, Paula Suares Oliveira, Tiago Almeida de Felizardo, Lucas Menezes |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Faria, Glaucia Amorim Peixoto, Ana Patricia Bastos Morais, Augusto Ramalho de Costa, Táis Ferreira Oliveira, Cintia Patrícia Martins de Lopes, Beatriz Garcia Rocha, Paula Suares Oliveira, Tiago Almeida de Felizardo, Lucas Menezes |
dc.subject.por.fl_str_mv |
Precisão experimental Coeficiente de variação Ensaio de uniformidade. Precisión experimental Coeficiente de variación Prueba de uniformidad. Experimental precision Coefficient of variation Uniformity test. |
topic |
Precisão experimental Coeficiente de variação Ensaio de uniformidade. Precisión experimental Coeficiente de variación Prueba de uniformidad. Experimental precision Coefficient of variation Uniformity test. |
description |
The genus Passiflora have great commercial, ornamental and phytotherapeutic importance. However, information on the ideal plot size in experiments aiming at the in vitro establishment of Passiflora is scarce. This study aimed at determining the optimal size of plots in experiments of in vitro establishment of the species Passiflora edulis Sims f. edulis, Passiflora giberti N. E. Brown. and Passiflora laurifolia L. For each species, uniformity tests were conducted with two concentrations of salts of the culture medium (MS and ½ MS) and for each test, evaluations were performed at 45, 75 and 105 days after incubation of the explants. In each evaluation and culture medium, different sizes of plots were simulated, ranging from one (1) basic unit to 50 per plot, with each basic unit consisting of a plant. To estimate the optimum plot size, the modified maximum curvature method was used. In in vitro establishment experiments, the recommended optimal plot size should be 11 explants for P. edulis Sims f. edulis, of 15 explants for P. giberti N. E. Brown. and 9 explants for P. laurifolia L. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-24 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/9354 10.33448/rsd-v9i10.9354 |
url |
https://rsdjournal.org/index.php/rsd/article/view/9354 |
identifier_str_mv |
10.33448/rsd-v9i10.9354 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/9354/8327 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 9 No. 10; e8859109354 Research, Society and Development; Vol. 9 Núm. 10; e8859109354 Research, Society and Development; v. 9 n. 10; e8859109354 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052781330169856 |