The composition of sacarine substrates for ethanol production and the fermentative capacity Saccharomyces cerevisiae Pedra-2
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/10235 |
Resumo: | The production of ethanol in Brazil is based on sugarcane juice, however other biomasses can be used for this process, such as sweet sorghum. However, some nutrients can interfere with fermentation, such as the presence of metals, carbon and nitrogen sources, which can affect the fermentation capacity of yeasts. Thus, this study aims to analyze the presence of fundamental nutrients present in saccharine substrates, as well as their assimilation and conversion of ethanol by the yeast Pedra-2. Samples of sugarcane and sorghum juice were obtained, in which analysis of the presence of metals was carried out using acid digestion and the levels determined by atomic flame absorption spectroscopy. The amino acid analysis was performed on the saccharine substrates at a concentration of 22 ºBrix, before and after fermentation, and analyzed by high-performance liquid chromatography and the concentration of ethanol by gas chromatography. The sorghum broth showed higher amounts of available amino acid metals. The yeast Pedra-2 showed better fermentative performance in the sorghum broth. We can conclude that the sorghum broth represents an important substrate to be used to increase the sustainability and production of ethanol in Brazil. |
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The composition of sacarine substrates for ethanol production and the fermentative capacity Saccharomyces cerevisiae Pedra-2La composición de sustratos de sacarina para la producción de etanol y la capacidad fermentativa da Saccharomyces cerevisiae Pedra-2A composição de substratos sacarinos para produção de etanol e a capacidade fermentativa da Saccharomyces cerevisiae edra-2NutrientsSugarcane juiceSweet sorghumFermentation.NutrientesJugo de caña de azúcarSorgo dulceFermentación.NutrientesCaldo de canaSorgo sacarinoFermentação.The production of ethanol in Brazil is based on sugarcane juice, however other biomasses can be used for this process, such as sweet sorghum. However, some nutrients can interfere with fermentation, such as the presence of metals, carbon and nitrogen sources, which can affect the fermentation capacity of yeasts. Thus, this study aims to analyze the presence of fundamental nutrients present in saccharine substrates, as well as their assimilation and conversion of ethanol by the yeast Pedra-2. Samples of sugarcane and sorghum juice were obtained, in which analysis of the presence of metals was carried out using acid digestion and the levels determined by atomic flame absorption spectroscopy. The amino acid analysis was performed on the saccharine substrates at a concentration of 22 ºBrix, before and after fermentation, and analyzed by high-performance liquid chromatography and the concentration of ethanol by gas chromatography. The sorghum broth showed higher amounts of available amino acid metals. The yeast Pedra-2 showed better fermentative performance in the sorghum broth. We can conclude that the sorghum broth represents an important substrate to be used to increase the sustainability and production of ethanol in Brazil.La producción de etanol en Brasil se basa en jugo de caña de azúcar, sin embargo se pueden utilizar otras biomasas para este proceso, como el sorgo dulce. Sin embargo, algunos nutrientes pueden interferir con la fermentación, como la presencia de fuentes de metales, carbono y nitrógeno que pueden afectar la capacidad de fermentación de las levaduras. Así, este estudio tiene como objetivo analizar la presencia de nutrientes fundamentales presentes en los sustratos sacarinos, así como su asimilación y conversión de etanol por la levadura Pedra-2. Se obtuvieron muestras de jugo de caña de azúcar y sorgo, en las cuales se realizaron análisis de presencia de metales mediante digestión ácida y los niveles determinados por espectroscopia de absorción de llama atómica. El análisis de aminoácidos se realizó sobre los sustratos dulces a una concentración de 22 ºBrix, antes y después de la fermentación, y se analizó por cromatografía líquida de alta resolución y la concentración de etanol por cromatografía de gases. El caldo de sorgo mostró mayores cantidades de metales aminoácidos disponibles. La levadura Pedra-2 mostró un mejor desempeño fermentativo en el caldo de sorgo. Podemos concluir que el jugo de sorgo representa un sustrato importante para ser utilizado para incrementar la sostenibilidad y producción de etanol en Brasil.A produção de etanol no Brasil tem como matéria-prima o caldo de cana-de-açúcar, contudo outras biomassas podem ser utilizadas para este processo, como o sorgo sacarino. Todavia, alguns nutrientes podem interferir na fermentação como a presença de metais, as fontes carbono e de nitrogênio que podem afetar a capacidade fermentativa das leveduras. Assim, este estudo visa analisar a presença nutrientes fundamentais presentes em substratos sacarinos, bem como sua assimilação e conversão de etanol pela levedura Pedra-2. Foram obtidas amostras de caldo de cana e sorgo, nas quais foram realizadas análises de presença de metais utilizando uma digestão ácida e os teores determinados por espectroscopia de absorção atômica de chama. A análise de aminoácidos foi realizada nos substratos sacarinos na concentração de 22 ºBrix, antes e após a fermentação, e analisadas por cromatografia líquida de alta eficiência e a concentração de etanol por cromatografia gasosa. O caldo de sorgo apresentou maiores quantidades de metais de aminoácidos disponíveis. A levedura Pedra-2 apresentou melhor desempenho fermentativo no caldo de sorgo. Podemos concluir que o caldo de sorgo representa um importante substrato a ser utilizado para aumentar a sustentabilidade e produção /do etanol no Brasil.Research, Society and Development2020-11-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1023510.33448/rsd-v9i11.10235Research, Society and Development; Vol. 9 No. 11; e44891110235Research, Society and Development; Vol. 9 Núm. 11; e44891110235Research, Society and Development; v. 9 n. 11; e448911102352525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/10235/8985Copyright (c) 2020 Rebeca Fasioli Silva; Maria do Socorro Mascarenhas Santos; Larissa Pires Mueller; Claudia Andrea Lima Cardoso; Margareth Batistotehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva, Rebeca Fasioli Santos, Maria do Socorro MascarenhasMueller, Larissa PiresCardoso, Claudia Andrea Lima Batistote, Margareth2020-12-10T23:37:57Zoai:ojs.pkp.sfu.ca:article/10235Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:32:20.327235Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
The composition of sacarine substrates for ethanol production and the fermentative capacity Saccharomyces cerevisiae Pedra-2 La composición de sustratos de sacarina para la producción de etanol y la capacidad fermentativa da Saccharomyces cerevisiae Pedra-2 A composição de substratos sacarinos para produção de etanol e a capacidade fermentativa da Saccharomyces cerevisiae edra-2 |
title |
The composition of sacarine substrates for ethanol production and the fermentative capacity Saccharomyces cerevisiae Pedra-2 |
spellingShingle |
The composition of sacarine substrates for ethanol production and the fermentative capacity Saccharomyces cerevisiae Pedra-2 Silva, Rebeca Fasioli Nutrients Sugarcane juice Sweet sorghum Fermentation. Nutrientes Jugo de caña de azúcar Sorgo dulce Fermentación. Nutrientes Caldo de cana Sorgo sacarino Fermentação. |
title_short |
The composition of sacarine substrates for ethanol production and the fermentative capacity Saccharomyces cerevisiae Pedra-2 |
title_full |
The composition of sacarine substrates for ethanol production and the fermentative capacity Saccharomyces cerevisiae Pedra-2 |
title_fullStr |
The composition of sacarine substrates for ethanol production and the fermentative capacity Saccharomyces cerevisiae Pedra-2 |
title_full_unstemmed |
The composition of sacarine substrates for ethanol production and the fermentative capacity Saccharomyces cerevisiae Pedra-2 |
title_sort |
The composition of sacarine substrates for ethanol production and the fermentative capacity Saccharomyces cerevisiae Pedra-2 |
author |
Silva, Rebeca Fasioli |
author_facet |
Silva, Rebeca Fasioli Santos, Maria do Socorro Mascarenhas Mueller, Larissa Pires Cardoso, Claudia Andrea Lima Batistote, Margareth |
author_role |
author |
author2 |
Santos, Maria do Socorro Mascarenhas Mueller, Larissa Pires Cardoso, Claudia Andrea Lima Batistote, Margareth |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Silva, Rebeca Fasioli Santos, Maria do Socorro Mascarenhas Mueller, Larissa Pires Cardoso, Claudia Andrea Lima Batistote, Margareth |
dc.subject.por.fl_str_mv |
Nutrients Sugarcane juice Sweet sorghum Fermentation. Nutrientes Jugo de caña de azúcar Sorgo dulce Fermentación. Nutrientes Caldo de cana Sorgo sacarino Fermentação. |
topic |
Nutrients Sugarcane juice Sweet sorghum Fermentation. Nutrientes Jugo de caña de azúcar Sorgo dulce Fermentación. Nutrientes Caldo de cana Sorgo sacarino Fermentação. |
description |
The production of ethanol in Brazil is based on sugarcane juice, however other biomasses can be used for this process, such as sweet sorghum. However, some nutrients can interfere with fermentation, such as the presence of metals, carbon and nitrogen sources, which can affect the fermentation capacity of yeasts. Thus, this study aims to analyze the presence of fundamental nutrients present in saccharine substrates, as well as their assimilation and conversion of ethanol by the yeast Pedra-2. Samples of sugarcane and sorghum juice were obtained, in which analysis of the presence of metals was carried out using acid digestion and the levels determined by atomic flame absorption spectroscopy. The amino acid analysis was performed on the saccharine substrates at a concentration of 22 ºBrix, before and after fermentation, and analyzed by high-performance liquid chromatography and the concentration of ethanol by gas chromatography. The sorghum broth showed higher amounts of available amino acid metals. The yeast Pedra-2 showed better fermentative performance in the sorghum broth. We can conclude that the sorghum broth represents an important substrate to be used to increase the sustainability and production of ethanol in Brazil. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-21 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/10235 10.33448/rsd-v9i11.10235 |
url |
https://rsdjournal.org/index.php/rsd/article/view/10235 |
identifier_str_mv |
10.33448/rsd-v9i11.10235 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/10235/8985 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 9 No. 11; e44891110235 Research, Society and Development; Vol. 9 Núm. 11; e44891110235 Research, Society and Development; v. 9 n. 11; e44891110235 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052664482103296 |