The composition of sacarine substrates for ethanol production and the fermentative capacity Saccharomyces cerevisiae Pedra-2

Detalhes bibliográficos
Autor(a) principal: Silva, Rebeca Fasioli
Data de Publicação: 2020
Outros Autores: Santos, Maria do Socorro Mascarenhas, Mueller, Larissa Pires, Cardoso, Claudia Andrea Lima, Batistote, Margareth
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/10235
Resumo: The production of ethanol in Brazil is based on sugarcane juice, however other biomasses can be used for this process, such as sweet sorghum. However, some nutrients can interfere with fermentation, such as the presence of metals, carbon and nitrogen sources, which can affect the fermentation capacity of yeasts. Thus, this study aims to analyze the presence of fundamental nutrients present in saccharine substrates, as well as their assimilation and conversion of ethanol by the yeast Pedra-2. Samples of sugarcane and sorghum juice were obtained, in which analysis of the presence of metals was carried out using acid digestion and the levels determined by atomic flame absorption spectroscopy. The amino acid analysis was performed on the saccharine substrates at a concentration of 22 ºBrix, before and after fermentation, and analyzed by high-performance liquid chromatography and the concentration of ethanol by gas chromatography. The sorghum broth showed higher amounts of available amino acid metals. The yeast Pedra-2 showed better fermentative performance in the sorghum broth. We can conclude that the sorghum broth represents an important substrate to be used to increase the sustainability and production of ethanol in Brazil.
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spelling The composition of sacarine substrates for ethanol production and the fermentative capacity Saccharomyces cerevisiae Pedra-2La composición de sustratos de sacarina para la producción de etanol y la capacidad fermentativa da Saccharomyces cerevisiae Pedra-2A composição de substratos sacarinos para produção de etanol e a capacidade fermentativa da Saccharomyces cerevisiae edra-2NutrientsSugarcane juiceSweet sorghumFermentation.NutrientesJugo de caña de azúcarSorgo dulceFermentación.NutrientesCaldo de canaSorgo sacarinoFermentação.The production of ethanol in Brazil is based on sugarcane juice, however other biomasses can be used for this process, such as sweet sorghum. However, some nutrients can interfere with fermentation, such as the presence of metals, carbon and nitrogen sources, which can affect the fermentation capacity of yeasts. Thus, this study aims to analyze the presence of fundamental nutrients present in saccharine substrates, as well as their assimilation and conversion of ethanol by the yeast Pedra-2. Samples of sugarcane and sorghum juice were obtained, in which analysis of the presence of metals was carried out using acid digestion and the levels determined by atomic flame absorption spectroscopy. The amino acid analysis was performed on the saccharine substrates at a concentration of 22 ºBrix, before and after fermentation, and analyzed by high-performance liquid chromatography and the concentration of ethanol by gas chromatography. The sorghum broth showed higher amounts of available amino acid metals. The yeast Pedra-2 showed better fermentative performance in the sorghum broth. We can conclude that the sorghum broth represents an important substrate to be used to increase the sustainability and production of ethanol in Brazil.La producción de etanol en Brasil se basa en jugo de caña de azúcar, sin embargo se pueden utilizar otras biomasas para este proceso, como el sorgo dulce. Sin embargo, algunos nutrientes pueden interferir con la fermentación, como la presencia de fuentes de metales, carbono y nitrógeno que pueden afectar la capacidad de fermentación de las levaduras. Así, este estudio tiene como objetivo analizar la presencia de nutrientes fundamentales presentes en los sustratos sacarinos, así como su asimilación y conversión de etanol por la levadura Pedra-2. Se obtuvieron muestras de jugo de caña de azúcar y sorgo, en las cuales se realizaron análisis de presencia de metales mediante digestión ácida y los niveles determinados por espectroscopia de absorción de llama atómica. El análisis de aminoácidos se realizó sobre los sustratos dulces a una concentración de 22 ºBrix, antes y después de la fermentación, y se analizó por cromatografía líquida de alta resolución y la concentración de etanol por cromatografía de gases. El caldo de sorgo mostró mayores cantidades de metales aminoácidos disponibles. La levadura Pedra-2 mostró un mejor desempeño fermentativo en el caldo de sorgo. Podemos concluir que el jugo de sorgo representa un sustrato importante para ser utilizado para incrementar la sostenibilidad y producción de etanol en Brasil.A produção de etanol no Brasil tem como matéria-prima o caldo de cana-de-açúcar, contudo outras biomassas podem ser utilizadas para este processo, como o sorgo sacarino. Todavia, alguns nutrientes podem interferir na fermentação como a presença de metais, as fontes carbono e de nitrogênio que podem afetar a capacidade fermentativa das leveduras. Assim, este estudo visa analisar a presença nutrientes fundamentais presentes em substratos sacarinos, bem como sua assimilação e conversão de etanol pela levedura Pedra-2. Foram obtidas amostras de caldo de cana e sorgo, nas quais foram realizadas análises de presença de metais utilizando uma digestão ácida e os teores determinados por espectroscopia de absorção atômica de chama. A análise de aminoácidos foi realizada nos substratos sacarinos na concentração de 22 ºBrix, antes e após a fermentação, e analisadas por cromatografia líquida de alta eficiência e a concentração de etanol por cromatografia gasosa. O caldo de sorgo apresentou maiores quantidades de metais de aminoácidos disponíveis. A levedura Pedra-2 apresentou melhor desempenho fermentativo no caldo de sorgo. Podemos concluir que o caldo de sorgo representa um importante substrato a ser utilizado para aumentar a sustentabilidade e produção /do etanol no Brasil.Research, Society and Development2020-11-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1023510.33448/rsd-v9i11.10235Research, Society and Development; Vol. 9 No. 11; e44891110235Research, Society and Development; Vol. 9 Núm. 11; e44891110235Research, Society and Development; v. 9 n. 11; e448911102352525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/10235/8985Copyright (c) 2020 Rebeca Fasioli Silva; Maria do Socorro Mascarenhas Santos; Larissa Pires Mueller; Claudia Andrea Lima Cardoso; Margareth Batistotehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva, Rebeca Fasioli Santos, Maria do Socorro MascarenhasMueller, Larissa PiresCardoso, Claudia Andrea Lima Batistote, Margareth2020-12-10T23:37:57Zoai:ojs.pkp.sfu.ca:article/10235Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:32:20.327235Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv The composition of sacarine substrates for ethanol production and the fermentative capacity Saccharomyces cerevisiae Pedra-2
La composición de sustratos de sacarina para la producción de etanol y la capacidad fermentativa da Saccharomyces cerevisiae Pedra-2
A composição de substratos sacarinos para produção de etanol e a capacidade fermentativa da Saccharomyces cerevisiae edra-2
title The composition of sacarine substrates for ethanol production and the fermentative capacity Saccharomyces cerevisiae Pedra-2
spellingShingle The composition of sacarine substrates for ethanol production and the fermentative capacity Saccharomyces cerevisiae Pedra-2
Silva, Rebeca Fasioli
Nutrients
Sugarcane juice
Sweet sorghum
Fermentation.
Nutrientes
Jugo de caña de azúcar
Sorgo dulce
Fermentación.
Nutrientes
Caldo de cana
Sorgo sacarino
Fermentação.
title_short The composition of sacarine substrates for ethanol production and the fermentative capacity Saccharomyces cerevisiae Pedra-2
title_full The composition of sacarine substrates for ethanol production and the fermentative capacity Saccharomyces cerevisiae Pedra-2
title_fullStr The composition of sacarine substrates for ethanol production and the fermentative capacity Saccharomyces cerevisiae Pedra-2
title_full_unstemmed The composition of sacarine substrates for ethanol production and the fermentative capacity Saccharomyces cerevisiae Pedra-2
title_sort The composition of sacarine substrates for ethanol production and the fermentative capacity Saccharomyces cerevisiae Pedra-2
author Silva, Rebeca Fasioli
author_facet Silva, Rebeca Fasioli
Santos, Maria do Socorro Mascarenhas
Mueller, Larissa Pires
Cardoso, Claudia Andrea Lima
Batistote, Margareth
author_role author
author2 Santos, Maria do Socorro Mascarenhas
Mueller, Larissa Pires
Cardoso, Claudia Andrea Lima
Batistote, Margareth
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Silva, Rebeca Fasioli
Santos, Maria do Socorro Mascarenhas
Mueller, Larissa Pires
Cardoso, Claudia Andrea Lima
Batistote, Margareth
dc.subject.por.fl_str_mv Nutrients
Sugarcane juice
Sweet sorghum
Fermentation.
Nutrientes
Jugo de caña de azúcar
Sorgo dulce
Fermentación.
Nutrientes
Caldo de cana
Sorgo sacarino
Fermentação.
topic Nutrients
Sugarcane juice
Sweet sorghum
Fermentation.
Nutrientes
Jugo de caña de azúcar
Sorgo dulce
Fermentación.
Nutrientes
Caldo de cana
Sorgo sacarino
Fermentação.
description The production of ethanol in Brazil is based on sugarcane juice, however other biomasses can be used for this process, such as sweet sorghum. However, some nutrients can interfere with fermentation, such as the presence of metals, carbon and nitrogen sources, which can affect the fermentation capacity of yeasts. Thus, this study aims to analyze the presence of fundamental nutrients present in saccharine substrates, as well as their assimilation and conversion of ethanol by the yeast Pedra-2. Samples of sugarcane and sorghum juice were obtained, in which analysis of the presence of metals was carried out using acid digestion and the levels determined by atomic flame absorption spectroscopy. The amino acid analysis was performed on the saccharine substrates at a concentration of 22 ºBrix, before and after fermentation, and analyzed by high-performance liquid chromatography and the concentration of ethanol by gas chromatography. The sorghum broth showed higher amounts of available amino acid metals. The yeast Pedra-2 showed better fermentative performance in the sorghum broth. We can conclude that the sorghum broth represents an important substrate to be used to increase the sustainability and production of ethanol in Brazil.
publishDate 2020
dc.date.none.fl_str_mv 2020-11-21
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/10235
10.33448/rsd-v9i11.10235
url https://rsdjournal.org/index.php/rsd/article/view/10235
identifier_str_mv 10.33448/rsd-v9i11.10235
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/10235/8985
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 9 No. 11; e44891110235
Research, Society and Development; Vol. 9 Núm. 11; e44891110235
Research, Society and Development; v. 9 n. 11; e44891110235
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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