Camellia sinensis (green tea) promotes antifungal activity against Candida spp. isolated from HIV-positive patients and biocompatibility in murine macrophages

Detalhes bibliográficos
Autor(a) principal: Monte, Marta Nicolini Falcão do
Data de Publicação: 2024
Outros Autores: Oliveira, Jonatas Rafael de, Oliveira, Luciane Dias de, Jorge , Antonio Olavo Cardoso
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/45002
Resumo: The aim of this study was to evaluate the antifungal activity and cytotoxicity of the plant extract from Camellia sinensis (green tea) in vitro, as well as to assess the antifungal effect of amphotericin B and fluconazole on 22 strains of Candida spp. isolated from the oral cavity of HIV-positive patients. The minimum fungicidal concentration (MFC) and minimum inhibitory concentration (MIC) were determined in planktonic cells using serial dilutions of green tea extract and antifungals. After determining the extract concentration at MIC and MFC, biofilm was prepared for each strain. Cytotoxicity in mouse macrophages (RAW 264.7) was evaluated to assess the cellular viability of this substance. Colony-forming units (CFU/mL) were counted, and the data were statistically evaluated using the Mann-Whitney test (p<0.05) for biofilm, visual observation for MIC and MFC, and ANOVA and Tukey for cytotoxicity. The results demonstrated viability of green tea extract in the analyzed cells. It was concluded in this study that C. sinensis (green tea) extract showed antifungal activity in planktonic cells and in biofilm for all Candida strains evaluated, with no cytotoxic effects on RAW 264.7. Fluconazole exhibited fungicidal effect in planktonic cells, while amphotericin B showed antifungal effect on C. albicans strains and microbial resistance in non-albicans strains.
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spelling Camellia sinensis (green tea) promotes antifungal activity against Candida spp. isolated from HIV-positive patients and biocompatibility in murine macrophagesCamellia sinensis (té verde) promueve actividad antifúngica contra Candida spp. aisladas de pacientes HIV positivos y biocompatibilidad en macrófagos murinosCamellia sinensis (chá verde) promove atividade antifúngica sobre Candida spp. isoladas de pacientes HIV-positivos e biocompatibilidade em macrófagos murinosAnfotericina BBiopelículasCamellia sinensisCandidaFluconazol.Anfotericina BBiofilmesCamellia sinensisCandidaFluconazol.Amphotericin BBiofilmsCamellia sinensisCandidaFluconazole.The aim of this study was to evaluate the antifungal activity and cytotoxicity of the plant extract from Camellia sinensis (green tea) in vitro, as well as to assess the antifungal effect of amphotericin B and fluconazole on 22 strains of Candida spp. isolated from the oral cavity of HIV-positive patients. The minimum fungicidal concentration (MFC) and minimum inhibitory concentration (MIC) were determined in planktonic cells using serial dilutions of green tea extract and antifungals. After determining the extract concentration at MIC and MFC, biofilm was prepared for each strain. Cytotoxicity in mouse macrophages (RAW 264.7) was evaluated to assess the cellular viability of this substance. Colony-forming units (CFU/mL) were counted, and the data were statistically evaluated using the Mann-Whitney test (p<0.05) for biofilm, visual observation for MIC and MFC, and ANOVA and Tukey for cytotoxicity. The results demonstrated viability of green tea extract in the analyzed cells. It was concluded in this study that C. sinensis (green tea) extract showed antifungal activity in planktonic cells and in biofilm for all Candida strains evaluated, with no cytotoxic effects on RAW 264.7. Fluconazole exhibited fungicidal effect in planktonic cells, while amphotericin B showed antifungal effect on C. albicans strains and microbial resistance in non-albicans strains.El objetivo de este estudio fue evaluar in vitro la actividad antifúngica y la citotoxicidad del extracto vegetal de Camellia sinensis (té verde), así como también evaluar el efecto antifúngico de la anfotericina B y el fluconazol en 22 cepas de Candida spp. aisladas de la cavidad oral de pacientes HIV positivos. Se determinaron la concentración mínima fungicida (CMF) y la concentración mínima inhibitoria (CMI) en células planctónicas utilizando diluciones seriadas del extracto de té verde y antifúngicos. Después de determinar la concentración del extracto en la CMI y la CMF, se preparó el biofilm de cada cepa. Se evaluó la citotoxicidad en macrófagos de ratón (RAW 264.7) para verificar la viabilidad celular de esta sustancia. Posteriormente, se realizó el recuento de unidades formadoras de colonias (UFC/mL) y los datos se evaluaron estadísticamente utilizando la prueba de Mann-Whitney (p <0.05) para las biopelículas, observación visual para CMI y CMF, ANOVA y Tukey para citotoxicidad. Los resultados demostraron viabilidad del extracto de té verde en las células analizadas. Se concluyó en este estudio que el extracto de C. sinensis (té verde) mostró actividad antifúngica en células planctónicas y en biopelículas para todas las cepas de Candida evaluadas, y no hubo efectos citotóxicos en RAW 264.7. El fluconazol mostró efecto fungicida en células planctónicas, mientras que la anfotericina B mostró efecto antifúngico en las cepas de C. albicans y resistencia microbiana en cepas no albicans.O objetivo deste trabalho foi avaliar in vitro a atividade antifúngica e a citotoxicidade do extrato vegetal de Camellia sinensis (chá verde) e também avaliar o efeito antifúngico da anfotericina B e fluconazol em 22 cepas de Candida spp. isoladas da cavidade bucal de pacientes HIV positivos. Foram determinadas as concentrações fungicida mínima e inibitória mínima (CFM e CIM) em células planctônicas, em diluições seriadas do extrato de chá verde e antifúngicos. Após determinação da concentração do extrato na CIM e na CFM, foi preparado o biofilme de cada cepa. Foi avaliada a citotoxicidade em macrófagos de camundongos (RAW 264.7), para verificar a viabilidade celular dessa substância. Posteriormente, foi realizada a contagem das unidades formadoras de colônia (UFC/mL) e os dados foram avaliados estatisticamente utilizando-se teste de Mann Whitney (p<0,05) para o biofilme, observação visual para CIM e CFM, Anova e Tukey para citotoxicidade. Os resultados demonstraram que houve viabilidade do extrato do chá verde nas células analisadas. Foi concluído no presente estudo que o extrato de C. sinensis (chá verde) apresentou atividade antifúngica em células planctônicas e em biofilme para todas as cepas de Candida avaliadas e não houve efeito citotóxicos para RAW 264.7. O fluconazol apresentou efeito fungicida em células planctônicas e a anfotericina B apresentou efeito antifúngico nas cepas de C. albicans e resistência microbiana nas cepas não albicans.Research, Society and Development2024-02-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/4500210.33448/rsd-v13i2.45002Research, Society and Development; Vol. 13 No. 2; e7413245002Research, Society and Development; Vol. 13 Núm. 2; e7413245002Research, Society and Development; v. 13 n. 2; e74132450022525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/45002/35956Copyright (c) 2024 Marta Nicolini Falcão do Monte; Jonatas Rafael de Oliveira; Luciane Dias de Oliveira; Antonio Olavo Cardoso Jorge https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMonte, Marta Nicolini Falcão do Oliveira, Jonatas Rafael de Oliveira, Luciane Dias de Jorge , Antonio Olavo Cardoso 2024-03-04T09:47:20Zoai:ojs.pkp.sfu.ca:article/45002Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-03-04T09:47:20Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Camellia sinensis (green tea) promotes antifungal activity against Candida spp. isolated from HIV-positive patients and biocompatibility in murine macrophages
Camellia sinensis (té verde) promueve actividad antifúngica contra Candida spp. aisladas de pacientes HIV positivos y biocompatibilidad en macrófagos murinos
Camellia sinensis (chá verde) promove atividade antifúngica sobre Candida spp. isoladas de pacientes HIV-positivos e biocompatibilidade em macrófagos murinos
title Camellia sinensis (green tea) promotes antifungal activity against Candida spp. isolated from HIV-positive patients and biocompatibility in murine macrophages
spellingShingle Camellia sinensis (green tea) promotes antifungal activity against Candida spp. isolated from HIV-positive patients and biocompatibility in murine macrophages
Monte, Marta Nicolini Falcão do
Anfotericina B
Biopelículas
Camellia sinensis
Candida
Fluconazol.
Anfotericina B
Biofilmes
Camellia sinensis
Candida
Fluconazol.
Amphotericin B
Biofilms
Camellia sinensis
Candida
Fluconazole.
title_short Camellia sinensis (green tea) promotes antifungal activity against Candida spp. isolated from HIV-positive patients and biocompatibility in murine macrophages
title_full Camellia sinensis (green tea) promotes antifungal activity against Candida spp. isolated from HIV-positive patients and biocompatibility in murine macrophages
title_fullStr Camellia sinensis (green tea) promotes antifungal activity against Candida spp. isolated from HIV-positive patients and biocompatibility in murine macrophages
title_full_unstemmed Camellia sinensis (green tea) promotes antifungal activity against Candida spp. isolated from HIV-positive patients and biocompatibility in murine macrophages
title_sort Camellia sinensis (green tea) promotes antifungal activity against Candida spp. isolated from HIV-positive patients and biocompatibility in murine macrophages
author Monte, Marta Nicolini Falcão do
author_facet Monte, Marta Nicolini Falcão do
Oliveira, Jonatas Rafael de
Oliveira, Luciane Dias de
Jorge , Antonio Olavo Cardoso
author_role author
author2 Oliveira, Jonatas Rafael de
Oliveira, Luciane Dias de
Jorge , Antonio Olavo Cardoso
author2_role author
author
author
dc.contributor.author.fl_str_mv Monte, Marta Nicolini Falcão do
Oliveira, Jonatas Rafael de
Oliveira, Luciane Dias de
Jorge , Antonio Olavo Cardoso
dc.subject.por.fl_str_mv Anfotericina B
Biopelículas
Camellia sinensis
Candida
Fluconazol.
Anfotericina B
Biofilmes
Camellia sinensis
Candida
Fluconazol.
Amphotericin B
Biofilms
Camellia sinensis
Candida
Fluconazole.
topic Anfotericina B
Biopelículas
Camellia sinensis
Candida
Fluconazol.
Anfotericina B
Biofilmes
Camellia sinensis
Candida
Fluconazol.
Amphotericin B
Biofilms
Camellia sinensis
Candida
Fluconazole.
description The aim of this study was to evaluate the antifungal activity and cytotoxicity of the plant extract from Camellia sinensis (green tea) in vitro, as well as to assess the antifungal effect of amphotericin B and fluconazole on 22 strains of Candida spp. isolated from the oral cavity of HIV-positive patients. The minimum fungicidal concentration (MFC) and minimum inhibitory concentration (MIC) were determined in planktonic cells using serial dilutions of green tea extract and antifungals. After determining the extract concentration at MIC and MFC, biofilm was prepared for each strain. Cytotoxicity in mouse macrophages (RAW 264.7) was evaluated to assess the cellular viability of this substance. Colony-forming units (CFU/mL) were counted, and the data were statistically evaluated using the Mann-Whitney test (p<0.05) for biofilm, visual observation for MIC and MFC, and ANOVA and Tukey for cytotoxicity. The results demonstrated viability of green tea extract in the analyzed cells. It was concluded in this study that C. sinensis (green tea) extract showed antifungal activity in planktonic cells and in biofilm for all Candida strains evaluated, with no cytotoxic effects on RAW 264.7. Fluconazole exhibited fungicidal effect in planktonic cells, while amphotericin B showed antifungal effect on C. albicans strains and microbial resistance in non-albicans strains.
publishDate 2024
dc.date.none.fl_str_mv 2024-02-18
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/45002
10.33448/rsd-v13i2.45002
url https://rsdjournal.org/index.php/rsd/article/view/45002
identifier_str_mv 10.33448/rsd-v13i2.45002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/45002/35956
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 13 No. 2; e7413245002
Research, Society and Development; Vol. 13 Núm. 2; e7413245002
Research, Society and Development; v. 13 n. 2; e7413245002
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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