Equine sarcoid in the southern region of Rio Grande do Sul: 20-year casuistry

Detalhes bibliográficos
Autor(a) principal: Venancio, Fabiano da Rosa
Data de Publicação: 2022
Outros Autores: Alberti, Taina dos Santos, Amaral , Lorena Alvariza, Zamboni, Rosimeri, Scheid, Haide Valeska, Ribeiro, Leonardo Clasen, Lima, Marcelo de, Schild, Ana Lucia
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/26704
Resumo: The objective of this work was to study the epidemiology and distribution of equine sarcoid lesions diagnosed at the Laboratório Regional de Diagnóstico of UFPel (LRD/UFPel) over a period of 20 years, as well as to determine the main types and, the molecular identify of the bovine papillomavirus (BPV) associated to the lesions. Cases received between 2000 and 2020 were retrieved from the laboratory's necropsy protocols. The paraffin blocks of each case were cut into 3-5µm sections, stained with hematoxylin and eosin (HE) and reassessed under an optical microscope. The samples sent to the laboratory in 2021 were fixed in 10% formalin for histopathology and immunohistochemistry and, an aliquot of frozen tissues were sent to the Laboratory of Virology and Immunology (LabVir/UFPel) for PCR. During the study period, 258 cases were diagnosed, of which 75.96% in the Crioula breed, mainly in horses aged two to five years (42.64%). The macroscopic characteristics associated with the histopathology allowed us to determine the type of sarcoid in each case. In 46.8% of the cases, the sarcoids were fibroblastic. Of the 258 cases, 61% were single, mainly observed in the limbs, and 32% were multiple, most often present in the trunk. The BPV genome was not detected by PCR and the IHC was negative for protein S-100 and positive for vimentin, allowing the differential diagnosis of other equine cutaneous neoplasms. The record of 258 cases of equine sarcoid for approximately 20 years has contributed significantly to understand its epidemiology in southern Rio Grande do Sul and revealed the importance of the disease in the studied region.
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spelling Equine sarcoid in the southern region of Rio Grande do Sul: 20-year casuistry Sarcoide equino en la región sur de Rio Grande do Sul: casuística de 20 añosSarcoide equino na região Sul do Rio Grande do Sul: casuística de 20 anosHistopathologyIHCSkin neoplasmBovine papillomavirusPCREquine sarcoid.HistopatologíaIHCNeoplasia de pielVirus del papiloma bovinoPCRSarcoide equino.HistopatologiaIHQNeoplasia cutâneaPapilomavírus bovinoPCRSarcoide equino.The objective of this work was to study the epidemiology and distribution of equine sarcoid lesions diagnosed at the Laboratório Regional de Diagnóstico of UFPel (LRD/UFPel) over a period of 20 years, as well as to determine the main types and, the molecular identify of the bovine papillomavirus (BPV) associated to the lesions. Cases received between 2000 and 2020 were retrieved from the laboratory's necropsy protocols. The paraffin blocks of each case were cut into 3-5µm sections, stained with hematoxylin and eosin (HE) and reassessed under an optical microscope. The samples sent to the laboratory in 2021 were fixed in 10% formalin for histopathology and immunohistochemistry and, an aliquot of frozen tissues were sent to the Laboratory of Virology and Immunology (LabVir/UFPel) for PCR. During the study period, 258 cases were diagnosed, of which 75.96% in the Crioula breed, mainly in horses aged two to five years (42.64%). The macroscopic characteristics associated with the histopathology allowed us to determine the type of sarcoid in each case. In 46.8% of the cases, the sarcoids were fibroblastic. Of the 258 cases, 61% were single, mainly observed in the limbs, and 32% were multiple, most often present in the trunk. The BPV genome was not detected by PCR and the IHC was negative for protein S-100 and positive for vimentin, allowing the differential diagnosis of other equine cutaneous neoplasms. The record of 258 cases of equine sarcoid for approximately 20 years has contributed significantly to understand its epidemiology in southern Rio Grande do Sul and revealed the importance of the disease in the studied region.Los objetivos de este trabajo fueron estudiar la epidemiología y distribución de las lesiones de sarcoidosis equina diagnosticadas en el Laboratorio Regional de Diagnóstico de la UFPel (LRD/UFPel) en un período de 20 años, así como determinar los principales tipos y, mediante PCR, identificar el virus del papiloma bovino (BPV) implicado en las lesiones. Los casos recibidos entre 2000 y 2020 se recuperaron de los protocolos de necropsia del laboratorio. Los bloques de parafina de cada caso se cortaron en secciones de 3-5 µm, se tiñeron con hematoxilina y eosina (HE) y se reevaluaron al microscopio óptico. Las muestras enviadas al laboratorio en 2021 fueron fijadas en formol al 10% para histopatología e inmunohistoquímica y parte fue congelada y enviada al Laboratorio de Virología e Inmunología de la UFPel (LabVir/UFPel) para PCR. En el período fueron diagnosticados 258 casos, de los cuales el 75,96% en la raza Crioula, principalmente en equinos de dos a cinco años (42,64%). Las características macroscópicas asociadas a la histopatología permitieron determinar el tipo de sarcoide en cada caso. En el 46,8% de los casos las sarcoides eran fibroblásticas. De los 258 casos, el 61% fueron únicos, principalmente observados en las extremidades, y el 32% fueron múltiples, con mayor frecuencia presentes en el tronco. El genoma del BPV no fue detectado por PCR y la IHC fue negativa para proteína S-100 y positiva para vimentina, lo que permitió el diagnóstico diferencial de otras neoplasias cutáneas equinas. El registro de 258 casos de sarcoide en 20 años permitió establecer su epidemiología en la región sur de Rio Grande do Sul y alerta sobre la importancia de la enfermedad en la región.Os objetivos deste trabalho foram estudar a epidemiologia e a distribuição das lesões de sarcoide equino diagnosticado no Laboratório Regional de Diagnóstico da UFPel (LRD/UFPel) em um período de 20 anos, bem como determinar os principais tipos e, por meio da PCR, identificar o papilomavirus bovino (BPV) envolvido nas lesões. Os casos recebidos entre 2000 e 2020 foram resgatados dos protocolos de necropsia do laboratório. Os blocos parafinados de cada caso foram cortados em secções de 3-5µm, corados por hematoxilina e eosina (HE) e reavaliados em microscópio ótico. As amostras encaminhadas ao laboratório em 2021 foram fixadas em formalina 10% para histopatologia e imuno-histoquímica e parte, foi congelada e encaminhada ao Laboratório de Virologia e Imunologia da UFPel (LabVir/UFPel) para realização da PCR. No período foram diagnosticados 258 casos, dos quais 75,96% na raça Crioula principalmente em equinos de dois a cinco anos (42,64%). As características macroscópicas associadas à histopatologia permitiram determinar o tipo de sarcoide em cada caso. Em 46,8% dos casos os sarcoides eram fibroblásticos. Dos 258 casos 61% eram únicos, observados principalmente nos membros e 32% eram múltiplos, presentes com mais frequência do tronco. O genoma dos BPVs não foi detectado pela PCR e a IHQ foi negativa para proteína S-100 e positiva para vimentina permitindo o diagnóstico diferencial de outras neoplasias cutâneas dos equinos. O registro de 258 casos de sarcoide em 20 anos permitiu estabelecer a sua epidemiologia na região Sul do Rio Grande do Sul e alerta para a importância da doença na região.Research, Society and Development2022-03-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2670410.33448/rsd-v11i3.26704Research, Society and Development; Vol. 11 No. 3; e43211326704Research, Society and Development; Vol. 11 Núm. 3; e43211326704Research, Society and Development; v. 11 n. 3; e432113267042525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/26704/23401Copyright (c) 2022 Fabiano da Rosa Venancio; Taina dos Santos Alberti; Lorena Alvariza Amaral ; Rosimeri Zamboni; Haide Valeska Scheid; Leonardo Clasen Ribeiro; Marcelo de Lima; Ana Lucia Schildhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessVenancio, Fabiano da Rosa Alberti, Taina dos SantosAmaral , Lorena Alvariza Zamboni, RosimeriScheid, Haide Valeska Ribeiro, Leonardo Clasen Lima, Marcelo de Schild, Ana Lucia 2022-03-09T13:44:38Zoai:ojs.pkp.sfu.ca:article/26704Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:44:40.701973Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Equine sarcoid in the southern region of Rio Grande do Sul: 20-year casuistry
Sarcoide equino en la región sur de Rio Grande do Sul: casuística de 20 años
Sarcoide equino na região Sul do Rio Grande do Sul: casuística de 20 anos
title Equine sarcoid in the southern region of Rio Grande do Sul: 20-year casuistry
spellingShingle Equine sarcoid in the southern region of Rio Grande do Sul: 20-year casuistry
Venancio, Fabiano da Rosa
Histopathology
IHC
Skin neoplasm
Bovine papillomavirus
PCR
Equine sarcoid.
Histopatología
IHC
Neoplasia de piel
Virus del papiloma bovino
PCR
Sarcoide equino.
Histopatologia
IHQ
Neoplasia cutânea
Papilomavírus bovino
PCR
Sarcoide equino.
title_short Equine sarcoid in the southern region of Rio Grande do Sul: 20-year casuistry
title_full Equine sarcoid in the southern region of Rio Grande do Sul: 20-year casuistry
title_fullStr Equine sarcoid in the southern region of Rio Grande do Sul: 20-year casuistry
title_full_unstemmed Equine sarcoid in the southern region of Rio Grande do Sul: 20-year casuistry
title_sort Equine sarcoid in the southern region of Rio Grande do Sul: 20-year casuistry
author Venancio, Fabiano da Rosa
author_facet Venancio, Fabiano da Rosa
Alberti, Taina dos Santos
Amaral , Lorena Alvariza
Zamboni, Rosimeri
Scheid, Haide Valeska
Ribeiro, Leonardo Clasen
Lima, Marcelo de
Schild, Ana Lucia
author_role author
author2 Alberti, Taina dos Santos
Amaral , Lorena Alvariza
Zamboni, Rosimeri
Scheid, Haide Valeska
Ribeiro, Leonardo Clasen
Lima, Marcelo de
Schild, Ana Lucia
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Venancio, Fabiano da Rosa
Alberti, Taina dos Santos
Amaral , Lorena Alvariza
Zamboni, Rosimeri
Scheid, Haide Valeska
Ribeiro, Leonardo Clasen
Lima, Marcelo de
Schild, Ana Lucia
dc.subject.por.fl_str_mv Histopathology
IHC
Skin neoplasm
Bovine papillomavirus
PCR
Equine sarcoid.
Histopatología
IHC
Neoplasia de piel
Virus del papiloma bovino
PCR
Sarcoide equino.
Histopatologia
IHQ
Neoplasia cutânea
Papilomavírus bovino
PCR
Sarcoide equino.
topic Histopathology
IHC
Skin neoplasm
Bovine papillomavirus
PCR
Equine sarcoid.
Histopatología
IHC
Neoplasia de piel
Virus del papiloma bovino
PCR
Sarcoide equino.
Histopatologia
IHQ
Neoplasia cutânea
Papilomavírus bovino
PCR
Sarcoide equino.
description The objective of this work was to study the epidemiology and distribution of equine sarcoid lesions diagnosed at the Laboratório Regional de Diagnóstico of UFPel (LRD/UFPel) over a period of 20 years, as well as to determine the main types and, the molecular identify of the bovine papillomavirus (BPV) associated to the lesions. Cases received between 2000 and 2020 were retrieved from the laboratory's necropsy protocols. The paraffin blocks of each case were cut into 3-5µm sections, stained with hematoxylin and eosin (HE) and reassessed under an optical microscope. The samples sent to the laboratory in 2021 were fixed in 10% formalin for histopathology and immunohistochemistry and, an aliquot of frozen tissues were sent to the Laboratory of Virology and Immunology (LabVir/UFPel) for PCR. During the study period, 258 cases were diagnosed, of which 75.96% in the Crioula breed, mainly in horses aged two to five years (42.64%). The macroscopic characteristics associated with the histopathology allowed us to determine the type of sarcoid in each case. In 46.8% of the cases, the sarcoids were fibroblastic. Of the 258 cases, 61% were single, mainly observed in the limbs, and 32% were multiple, most often present in the trunk. The BPV genome was not detected by PCR and the IHC was negative for protein S-100 and positive for vimentin, allowing the differential diagnosis of other equine cutaneous neoplasms. The record of 258 cases of equine sarcoid for approximately 20 years has contributed significantly to understand its epidemiology in southern Rio Grande do Sul and revealed the importance of the disease in the studied region.
publishDate 2022
dc.date.none.fl_str_mv 2022-03-02
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/26704
10.33448/rsd-v11i3.26704
url https://rsdjournal.org/index.php/rsd/article/view/26704
identifier_str_mv 10.33448/rsd-v11i3.26704
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/26704/23401
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 3; e43211326704
Research, Society and Development; Vol. 11 Núm. 3; e43211326704
Research, Society and Development; v. 11 n. 3; e43211326704
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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