Serum biochemical panel and modulation of skeletal muscle fibres: effect on the meat quality from lambs fed with different levels of whole cottonseed
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/28670 |
Resumo: | The use of whole cottonseed (WCS) in the diet of ruminants has come to prominence mainly because this ingredient contains high levels of protein and lipids. The aims of this study were to evaluate serum biochemical panel and modulation of the skeletal muscle fibres (Longissimus thoracis) of Ile de France lambs fed with levels of WCS; and relationship between skeletal muscle fibres and meat quality. Fifty lambs were used in the experiment: five (5) reference and 45 equally distributed in treatments 0, 10, 20, 30 and 40% WCS (64 days feedlot time), using a completely randomised design. Blood biochemical determinations were performed using colorimetric kits. The fibers were determined by histochemistry method and classified as Type I, Type IIA, Type IIB and Type IIC. Muscle fiber characteristics were correlated with meat quality, considering variables of color, texture and sensory profile, physicochemical and fatty acid profile. Cholesterol levels, total protein and gamma-glutamyl transferase increased (p≤0.05) in line with increased levels of WCS in the diet. Muscle fibres modulation (p≤0.05) was observed, with an increase in red, a reduction in glycolytic, and modulation of Type IIB to Type IIA. The addition of WCS to the diet increased (p≤0.05) the relative area of Type IIC. There was correlation between muscle fibres and meat qualitative characteristics. Time effect was more pronounced than diet effect on the muscle fibres modulation. Muscle fibres modulation was closely linked with meat quality. |
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Serum biochemical panel and modulation of skeletal muscle fibres: effect on the meat quality from lambs fed with different levels of whole cottonseedPanel bioquímico sérico y modulación de fibras musculares esqueléticas: efecto sobre la calidad de la carne de corderos alimentados con diferentes niveles de semilla de algodón enteraPainel bioquímico sérico e modulação de fibras musculares esqueléticas: efeito na qualidade da carne de cordeiros alimentados com níveis distintos de caroço de algodãoHistochemistryMyosin ATPaseMeat qualityLongissimus thoracis.HistoquímicaMiosina ATPasaCalidad de la carneLongissimus thoracis.ATPase miosínicaHistoquímicaQualidade da carneLongissimus thoracis.The use of whole cottonseed (WCS) in the diet of ruminants has come to prominence mainly because this ingredient contains high levels of protein and lipids. The aims of this study were to evaluate serum biochemical panel and modulation of the skeletal muscle fibres (Longissimus thoracis) of Ile de France lambs fed with levels of WCS; and relationship between skeletal muscle fibres and meat quality. Fifty lambs were used in the experiment: five (5) reference and 45 equally distributed in treatments 0, 10, 20, 30 and 40% WCS (64 days feedlot time), using a completely randomised design. Blood biochemical determinations were performed using colorimetric kits. The fibers were determined by histochemistry method and classified as Type I, Type IIA, Type IIB and Type IIC. Muscle fiber characteristics were correlated with meat quality, considering variables of color, texture and sensory profile, physicochemical and fatty acid profile. Cholesterol levels, total protein and gamma-glutamyl transferase increased (p≤0.05) in line with increased levels of WCS in the diet. Muscle fibres modulation (p≤0.05) was observed, with an increase in red, a reduction in glycolytic, and modulation of Type IIB to Type IIA. The addition of WCS to the diet increased (p≤0.05) the relative area of Type IIC. There was correlation between muscle fibres and meat qualitative characteristics. Time effect was more pronounced than diet effect on the muscle fibres modulation. Muscle fibres modulation was closely linked with meat quality.El uso de la semilla de algodón entera (SAE) en la dieta de los rumiantes se ha destacado principalmente porque este ingrediente contiene altos niveles de proteínas y lípidos. Los objetivos de este estudio fueron evaluar el panel bioquímico sérico y la modulación de las fibras del músculo esquelético (Longissimus thoracis) de corderos Ile de France alimentados con niveles de SAE; y relación entre las fibras musculares esqueléticas y la calidad de la carne. En el experimento se utilizaron 50 corderos: cinco (5) de referencia y 45 distribuidos equitativamente en los tratamientos 0, 10, 20, 30 y 40% SAE (tiempo de engorde de 64 días), utilizando un diseño completamente al azar. Las determinaciones bioquímicas sanguíneas se realizaron utilizando kits colorimétricos. Las fibras se determinaron por método histoquímico y se clasificaron en Tipo I, Tipo IIA, Tipo IIB y Tipo IIC. Las características de la fibra muscular se correlacionaron con la calidad de la carne, considerando variables de color, textura y perfil sensorial, fisicoquímico y perfil de ácidos grasos. Los niveles de colesterol, proteína total y gamma-glutamil transferasa aumentaron (p≤0.05) en consonancia con el aumento de los niveles de SAE en la dieta. Se observó modulación de las fibras musculares (p≤0.05), con aumento de rojo, reducción de glucolíticos y modulación de Tipo IIB a Tipo IIA. La adición de SAE a la dieta incrementó (p≤0.05) el área relativa de Tipo IIC. Hubo correlación entre las fibras musculares y las características cualitativas de la carne. El efecto del tiempo fue más pronunciado que el efecto de la dieta en la modulación de las fibras musculares. La modulación de las fibras musculares estuvo estrechamente relacionada con la calidad de la carne.A utilização de caroço de algodão (CA) na dieta de ruminantes tem ganhado destaque principalmente por este ingrediente conter altos teores de proteínas e lipídios. Objetivou-se avaliar o painel bioquímico sérico e a modulação das fibras musculares esqueléticas (Longissimus thoracis) de cordeiros Ile de France alimentados com níveis de CA; e a relação entre fibras musculares esqueléticas e qualidade da carne. Foram utilizados 50 cordeiros: cinco (5) referência e 45 igualmente distribuídos nos tratamentos 0, 10, 20, 30 e 40% CA (64 dias de confinamento), em delineamento inteiramente casualizado. As análises bioquímicas no sangue foram realizadas utilizando kits colorimétricos. As fibras foram determinadas pelo método histoquímico e classificadas como Tipo I, Tipo IIA, Tipo IIB e Tipo IIC. As características das fibras musculares foram correlacionadas com a qualidade da carne, considerando variáveis de cor, perfis de textura e sensorial, físico-química e perfil de ácidos graxos. Os teores de colesterol, proteína total e gama-glutamil transferase aumentaram (p≤0,05) com o incremento de CA na dieta. Verificou-se modulação das fibras musculares (p≤0,05), com aumento das vermelhas, redução das glicolíticas e modulação do Tipo IIB para Tipo IIA. A inclusão de CA na dieta aumentou (p≤0,05) a área relativa do Tipo IIC. Houve correlação entre fibras musculares e características qualitativas da carne. O efeito de tempo foi mais pronunciado que o efeito de dieta na modulação das fibras musculares. A modulação das fibras musculares esteve intimamente ligada à qualidade da carne.Research, Society and Development2022-04-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2867010.33448/rsd-v11i6.28670Research, Society and Development; Vol. 11 No. 6; e6811628670Research, Society and Development; Vol. 11 Núm. 6; e6811628670Research, Society and Development; v. 11 n. 6; e68116286702525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/28670/24998Copyright (c) 2022 Magda Aita Monego ; Luiz Giovani de Pellegrini ; Amarylis Claudine Bonito Azeredo Wanschel ; Tatiana Emanuelli; Juliana Azevedo Lima Pallone ; Rosa Cristina Prestes Dornelles ; Ernesto Hashime Kubota ; Fernanda Cristina Breda ; Renius Mellohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMonego , Magda Aita Pellegrini , Luiz Giovani de Wanschel , Amarylis Claudine Bonito Azeredo Emanuelli, TatianaPallone , Juliana Azevedo Lima Dornelles , Rosa Cristina Prestes Kubota , Ernesto Hashime Breda , Fernanda Cristina Mello, Renius2022-05-13T18:04:10Zoai:ojs.pkp.sfu.ca:article/28670Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:45:59.669118Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Serum biochemical panel and modulation of skeletal muscle fibres: effect on the meat quality from lambs fed with different levels of whole cottonseed Panel bioquímico sérico y modulación de fibras musculares esqueléticas: efecto sobre la calidad de la carne de corderos alimentados con diferentes niveles de semilla de algodón entera Painel bioquímico sérico e modulação de fibras musculares esqueléticas: efeito na qualidade da carne de cordeiros alimentados com níveis distintos de caroço de algodão |
title |
Serum biochemical panel and modulation of skeletal muscle fibres: effect on the meat quality from lambs fed with different levels of whole cottonseed |
spellingShingle |
Serum biochemical panel and modulation of skeletal muscle fibres: effect on the meat quality from lambs fed with different levels of whole cottonseed Monego , Magda Aita Histochemistry Myosin ATPase Meat quality Longissimus thoracis. Histoquímica Miosina ATPasa Calidad de la carne Longissimus thoracis. ATPase miosínica Histoquímica Qualidade da carne Longissimus thoracis. |
title_short |
Serum biochemical panel and modulation of skeletal muscle fibres: effect on the meat quality from lambs fed with different levels of whole cottonseed |
title_full |
Serum biochemical panel and modulation of skeletal muscle fibres: effect on the meat quality from lambs fed with different levels of whole cottonseed |
title_fullStr |
Serum biochemical panel and modulation of skeletal muscle fibres: effect on the meat quality from lambs fed with different levels of whole cottonseed |
title_full_unstemmed |
Serum biochemical panel and modulation of skeletal muscle fibres: effect on the meat quality from lambs fed with different levels of whole cottonseed |
title_sort |
Serum biochemical panel and modulation of skeletal muscle fibres: effect on the meat quality from lambs fed with different levels of whole cottonseed |
author |
Monego , Magda Aita |
author_facet |
Monego , Magda Aita Pellegrini , Luiz Giovani de Wanschel , Amarylis Claudine Bonito Azeredo Emanuelli, Tatiana Pallone , Juliana Azevedo Lima Dornelles , Rosa Cristina Prestes Kubota , Ernesto Hashime Breda , Fernanda Cristina Mello, Renius |
author_role |
author |
author2 |
Pellegrini , Luiz Giovani de Wanschel , Amarylis Claudine Bonito Azeredo Emanuelli, Tatiana Pallone , Juliana Azevedo Lima Dornelles , Rosa Cristina Prestes Kubota , Ernesto Hashime Breda , Fernanda Cristina Mello, Renius |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Monego , Magda Aita Pellegrini , Luiz Giovani de Wanschel , Amarylis Claudine Bonito Azeredo Emanuelli, Tatiana Pallone , Juliana Azevedo Lima Dornelles , Rosa Cristina Prestes Kubota , Ernesto Hashime Breda , Fernanda Cristina Mello, Renius |
dc.subject.por.fl_str_mv |
Histochemistry Myosin ATPase Meat quality Longissimus thoracis. Histoquímica Miosina ATPasa Calidad de la carne Longissimus thoracis. ATPase miosínica Histoquímica Qualidade da carne Longissimus thoracis. |
topic |
Histochemistry Myosin ATPase Meat quality Longissimus thoracis. Histoquímica Miosina ATPasa Calidad de la carne Longissimus thoracis. ATPase miosínica Histoquímica Qualidade da carne Longissimus thoracis. |
description |
The use of whole cottonseed (WCS) in the diet of ruminants has come to prominence mainly because this ingredient contains high levels of protein and lipids. The aims of this study were to evaluate serum biochemical panel and modulation of the skeletal muscle fibres (Longissimus thoracis) of Ile de France lambs fed with levels of WCS; and relationship between skeletal muscle fibres and meat quality. Fifty lambs were used in the experiment: five (5) reference and 45 equally distributed in treatments 0, 10, 20, 30 and 40% WCS (64 days feedlot time), using a completely randomised design. Blood biochemical determinations were performed using colorimetric kits. The fibers were determined by histochemistry method and classified as Type I, Type IIA, Type IIB and Type IIC. Muscle fiber characteristics were correlated with meat quality, considering variables of color, texture and sensory profile, physicochemical and fatty acid profile. Cholesterol levels, total protein and gamma-glutamyl transferase increased (p≤0.05) in line with increased levels of WCS in the diet. Muscle fibres modulation (p≤0.05) was observed, with an increase in red, a reduction in glycolytic, and modulation of Type IIB to Type IIA. The addition of WCS to the diet increased (p≤0.05) the relative area of Type IIC. There was correlation between muscle fibres and meat qualitative characteristics. Time effect was more pronounced than diet effect on the muscle fibres modulation. Muscle fibres modulation was closely linked with meat quality. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-20 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/28670 10.33448/rsd-v11i6.28670 |
url |
https://rsdjournal.org/index.php/rsd/article/view/28670 |
identifier_str_mv |
10.33448/rsd-v11i6.28670 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/28670/24998 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 6; e6811628670 Research, Society and Development; Vol. 11 Núm. 6; e6811628670 Research, Society and Development; v. 11 n. 6; e6811628670 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052710453772288 |