In vitro study after exposure to the aqueous extract of Piper amalago L. shows changes of morphology, proliferation, cytoskeleton and molecules of the extracellular matrix

Detalhes bibliográficos
Autor(a) principal: Santos, Vera Lucia Pereira dos
Data de Publicação: 2021
Outros Autores: Franco, Célia Regina Cavichiolo, Wagner, Ricardo, Silva, Caroline Dadalt, Santos, Giane Favretto dos, Cunha, Regiane Stafim da, Stinghen, Andréa Emilia Marques, Monteiro, Luciane Mendes, Bussade, Julia Emília, Budel, Jane Manfron, Messias-Reason, Iara José de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/13289
Resumo: Piper amalago L. is a medicinal plant traditionally used as a healing agent for wounds, burns, abscesses, boils, and insect bites. The current study aimed to evaluate the possible effects of the aqueous crude extract obtained from P. amalago leaves, in different concentrations and in different incubation times, using the in vitro model of mouse fibroblasts (3T3). The extract was tested in different concentrations at the 24 h incubation time for analysis of cell viability, cytotoxicity, proliferation, cell morphology, immunostaining, adhesion and cell spreading assays, as well as to determine the hydroxyproline concentration and activity of the metalloproteinase MMP2. Morphologically, after exposure to the concentrations of 15 and 150 µg/mL, the cells maintained the morphology, yet a greater number of cells with more expansions of the cell body and larger than the control cells were observed. The treated cell culture also showed a greater number of cells, larger cells, a greater expansion of the cell body, adherent cells spread over the substrate, and a more juxtaposed, central and spherical nucleus. The treatment induced greater cell adhesion to the polymer, fibronectin, and collagen I. Biochemical results showed a significant increase in the hydroxyproline amino acid after exposure for 96 h. The extract did not induce loss of cell viability until the concentration reached 150 µg/mL, positively modulating proliferation, morphology, adhesion, degree of spreading, and organization of microfilaments. The extract also promoted a significant increase in the hydroxyproline amino acid.
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spelling In vitro study after exposure to the aqueous extract of Piper amalago L. shows changes of morphology, proliferation, cytoskeleton and molecules of the extracellular matrixEstudio in vitro después de la exposición al extracto acuoso de Piper amalago L. muestra los cambios en la morfología, proliferación, citoesqueleto y moléculas de la matriz extracelularEstudo in vitro após a exposição ao extrato aquoso de Piper amalago L. mostra alterações de morfologia, proliferação, citosesqueleto e moléculas da matriz extracelularFibroblastoJaborandi-mansoLinhagem 3T3.FibroblastoJaborandi-mansoLinaje 3T3.FibroblastJaborandi-manso3T3 strain.Piper amalago L. is a medicinal plant traditionally used as a healing agent for wounds, burns, abscesses, boils, and insect bites. The current study aimed to evaluate the possible effects of the aqueous crude extract obtained from P. amalago leaves, in different concentrations and in different incubation times, using the in vitro model of mouse fibroblasts (3T3). The extract was tested in different concentrations at the 24 h incubation time for analysis of cell viability, cytotoxicity, proliferation, cell morphology, immunostaining, adhesion and cell spreading assays, as well as to determine the hydroxyproline concentration and activity of the metalloproteinase MMP2. Morphologically, after exposure to the concentrations of 15 and 150 µg/mL, the cells maintained the morphology, yet a greater number of cells with more expansions of the cell body and larger than the control cells were observed. The treated cell culture also showed a greater number of cells, larger cells, a greater expansion of the cell body, adherent cells spread over the substrate, and a more juxtaposed, central and spherical nucleus. The treatment induced greater cell adhesion to the polymer, fibronectin, and collagen I. Biochemical results showed a significant increase in the hydroxyproline amino acid after exposure for 96 h. The extract did not induce loss of cell viability until the concentration reached 150 µg/mL, positively modulating proliferation, morphology, adhesion, degree of spreading, and organization of microfilaments. The extract also promoted a significant increase in the hydroxyproline amino acid.Piper amalago L. es una planta medicinal utilizada tradicionalmente como agente cicatrizante de heridas, quemaduras, abscesos, furúnculos y picaduras de insectos. El presente estudio tuvo como objetivo evaluar los posibles efectos del extracto crudo acuoso obtenido de las hojas de P. amalago, en diferentes concentraciones y en diferentes tiempos de incubación, utilizando el modelo in vitro de fibroblastos de ratón (3T3). El extracto se probó a diferentes concentraciones en el tiempo de incubación de 24 horas para el análisis de viabilidad celular, citotoxicidad, proliferación, morfología celular, inmunotinción, ensayos de adhesión y propagación celular, así como para determinar la concentración de hidroxiprolina y la actividad metaloproteinasa de MMP2. Morfológicamente, tras la exposición a concentraciones de 15 y 150 µg/mL, las células mantuvieron su morfología, pero se observó un mayor número de células con más expansiones del cuerpo celular y más grandes que las células control. El cultivo celular tratado también mostró un mayor número de células, células más grandes, mayor expansión del cuerpo celular, células adherentes difundidas sobre el sustrato y núcleos más yuxtapuestos, centrales y esféricos. El tratamiento indujo una mayor adhesión celular al polímero, fibronectina y colágeno I. Los resultados bioquímicos mostraron un aumento significativo en el aminoácido hidroxiprolina después de 96 h de exposición. El extracto no indujo pérdida de viabilidad celular hasta que la concentración alcanzó 150 µg/mL, modulando positivamente la proliferación, morfología, adhesión, grado de diseminación y organización de los microfilamentos. El extracto también promovió un aumento significativo del aminoácido hidroxiprolina.Piper amalago L. é uma planta medicinal tradicionalmente usada como agente de cura para feridas, queimaduras, abcessos, furúnculos e picadas de insetos. O presente estudo teve como objetivo avaliar os possíveis efeitos do extrato bruto aquoso obtido das folhas de P. amalago, em diferentes concentrações e em diferentes tempos de incubação, utilizando o modelo in vitro de fibroblastos de camundongo (3T3). O extrato foi testado em diferentes concentrações no tempo de incubação de 24 horas para análise de viabilidade celular, citotoxicidade, proliferação, morfologia celular, imunomarcação, adesão e ensaios de propagação celular, bem como para determinar a concentração de hidroxiprolina e atividade da metaloproteinase MMP2. Morfologicamente, após exposição às concentrações de 15 e 150 µg/mL, as células mantiveram a morfologia, porém foi observado um número maior de células com mais expansões do corpo celular e maiores que as células controle. A cultura celular tratada também apresentou maior número de células, células maiores, maior expansão do corpo celular, células aderentes espalhadas pelo substrato e núcleo mais justaposto, central e esférico. O tratamento induziu maior adesão celular ao polímero, fibronectina e colágeno I. Os resultados bioquímicos mostraram um aumento significativo no aminoácido hidroxiprolina após exposição por 96 h. O extrato não induziu perda de viabilidade celular até a concentração atingir 150 µg/mL, modulando positivamente a proliferação, morfologia, adesão, grau de disseminação e organização dos microfilamentos. O extrato também promoveu um aumento significativo no aminoácido hidroxiprolina.Research, Society and Development2021-03-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1328910.33448/rsd-v10i4.13289Research, Society and Development; Vol. 10 No. 4; e0110413289Research, Society and Development; Vol. 10 Núm. 4; e0110413289Research, Society and Development; v. 10 n. 4; e01104132892525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/13289/12408Copyright (c) 2021 Vera Lucia Pereira dos Santos; Célia Regina Cavichiolo Franco; Ricardo Wagner; Caroline Dadalt Silva; Célia Regina Cavichiolo Franco; Ricardo Wagner; Caroline Dadalt Silva; Giane Favretto dos Santos; Regiane Stafim da Cunha; Andréa Emilia Marques Stinghen; Luciane Mendes Monteiro; Julia Emília Bussade; Jane Manfron Budel; Iara José de Messias-Reasonhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSantos, Vera Lucia Pereira dos Franco, Célia Regina Cavichiolo Wagner, RicardoSilva, Caroline Dadalt Franco, Célia Regina Cavichiolo Wagner, RicardoSilva, Caroline Dadalt Santos, Giane Favretto dos Cunha, Regiane Stafim da Stinghen, Andréa Emilia Marques Monteiro, Luciane Mendes Bussade, Julia Emília Budel, Jane Manfron Messias-Reason, Iara José de 2021-04-25T11:21:26Zoai:ojs.pkp.sfu.ca:article/13289Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:34:40.261187Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv In vitro study after exposure to the aqueous extract of Piper amalago L. shows changes of morphology, proliferation, cytoskeleton and molecules of the extracellular matrix
Estudio in vitro después de la exposición al extracto acuoso de Piper amalago L. muestra los cambios en la morfología, proliferación, citoesqueleto y moléculas de la matriz extracelular
Estudo in vitro após a exposição ao extrato aquoso de Piper amalago L. mostra alterações de morfologia, proliferação, citosesqueleto e moléculas da matriz extracelular
title In vitro study after exposure to the aqueous extract of Piper amalago L. shows changes of morphology, proliferation, cytoskeleton and molecules of the extracellular matrix
spellingShingle In vitro study after exposure to the aqueous extract of Piper amalago L. shows changes of morphology, proliferation, cytoskeleton and molecules of the extracellular matrix
Santos, Vera Lucia Pereira dos
Fibroblasto
Jaborandi-manso
Linhagem 3T3.
Fibroblasto
Jaborandi-manso
Linaje 3T3.
Fibroblast
Jaborandi-manso
3T3 strain.
title_short In vitro study after exposure to the aqueous extract of Piper amalago L. shows changes of morphology, proliferation, cytoskeleton and molecules of the extracellular matrix
title_full In vitro study after exposure to the aqueous extract of Piper amalago L. shows changes of morphology, proliferation, cytoskeleton and molecules of the extracellular matrix
title_fullStr In vitro study after exposure to the aqueous extract of Piper amalago L. shows changes of morphology, proliferation, cytoskeleton and molecules of the extracellular matrix
title_full_unstemmed In vitro study after exposure to the aqueous extract of Piper amalago L. shows changes of morphology, proliferation, cytoskeleton and molecules of the extracellular matrix
title_sort In vitro study after exposure to the aqueous extract of Piper amalago L. shows changes of morphology, proliferation, cytoskeleton and molecules of the extracellular matrix
author Santos, Vera Lucia Pereira dos
author_facet Santos, Vera Lucia Pereira dos
Franco, Célia Regina Cavichiolo
Wagner, Ricardo
Silva, Caroline Dadalt
Santos, Giane Favretto dos
Cunha, Regiane Stafim da
Stinghen, Andréa Emilia Marques
Monteiro, Luciane Mendes
Bussade, Julia Emília
Budel, Jane Manfron
Messias-Reason, Iara José de
author_role author
author2 Franco, Célia Regina Cavichiolo
Wagner, Ricardo
Silva, Caroline Dadalt
Santos, Giane Favretto dos
Cunha, Regiane Stafim da
Stinghen, Andréa Emilia Marques
Monteiro, Luciane Mendes
Bussade, Julia Emília
Budel, Jane Manfron
Messias-Reason, Iara José de
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Vera Lucia Pereira dos
Franco, Célia Regina Cavichiolo
Wagner, Ricardo
Silva, Caroline Dadalt
Franco, Célia Regina Cavichiolo
Wagner, Ricardo
Silva, Caroline Dadalt
Santos, Giane Favretto dos
Cunha, Regiane Stafim da
Stinghen, Andréa Emilia Marques
Monteiro, Luciane Mendes
Bussade, Julia Emília
Budel, Jane Manfron
Messias-Reason, Iara José de
dc.subject.por.fl_str_mv Fibroblasto
Jaborandi-manso
Linhagem 3T3.
Fibroblasto
Jaborandi-manso
Linaje 3T3.
Fibroblast
Jaborandi-manso
3T3 strain.
topic Fibroblasto
Jaborandi-manso
Linhagem 3T3.
Fibroblasto
Jaborandi-manso
Linaje 3T3.
Fibroblast
Jaborandi-manso
3T3 strain.
description Piper amalago L. is a medicinal plant traditionally used as a healing agent for wounds, burns, abscesses, boils, and insect bites. The current study aimed to evaluate the possible effects of the aqueous crude extract obtained from P. amalago leaves, in different concentrations and in different incubation times, using the in vitro model of mouse fibroblasts (3T3). The extract was tested in different concentrations at the 24 h incubation time for analysis of cell viability, cytotoxicity, proliferation, cell morphology, immunostaining, adhesion and cell spreading assays, as well as to determine the hydroxyproline concentration and activity of the metalloproteinase MMP2. Morphologically, after exposure to the concentrations of 15 and 150 µg/mL, the cells maintained the morphology, yet a greater number of cells with more expansions of the cell body and larger than the control cells were observed. The treated cell culture also showed a greater number of cells, larger cells, a greater expansion of the cell body, adherent cells spread over the substrate, and a more juxtaposed, central and spherical nucleus. The treatment induced greater cell adhesion to the polymer, fibronectin, and collagen I. Biochemical results showed a significant increase in the hydroxyproline amino acid after exposure for 96 h. The extract did not induce loss of cell viability until the concentration reached 150 µg/mL, positively modulating proliferation, morphology, adhesion, degree of spreading, and organization of microfilaments. The extract also promoted a significant increase in the hydroxyproline amino acid.
publishDate 2021
dc.date.none.fl_str_mv 2021-03-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/13289
10.33448/rsd-v10i4.13289
url https://rsdjournal.org/index.php/rsd/article/view/13289
identifier_str_mv 10.33448/rsd-v10i4.13289
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/13289/12408
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 4; e0110413289
Research, Society and Development; Vol. 10 Núm. 4; e0110413289
Research, Society and Development; v. 10 n. 4; e0110413289
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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