Physiological response to the action of the agrotoxic 2,4-Dichlorophenoxiacetic acid in Saccharomyces cerevisiae
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/19912 |
Resumo: | The presence of xenobiotic compounds in the environment is responsible for impacts on the ecosystem. An example is pesticides that pose risks to non-target species, such as microorganisms present in the soil and that are responsible for cycling nutrients, some can be used to measure the effects of these compounds, such as yeasts because when exposed to toxic substances begin to present changes in physiological and genetic mechanisms. Thus, this study aims to evaluate the toxicity effect of the 2,4-D pesticide on Pedra-2 (PE-2) and Fleischmann® (FLE) yeasts. 2,4-D dilutions of 2.0; 4.0 and 6.0 µg L-1 were added to a solution prepared with 20 ml of ultra-pure water and 2.0 g of sucrose where the yeasts were grown. The phenotypic profile of yeasts against the toxicity of the compound was evaluated quantitatively with cell viability. Using the methylene blue method and qualitatively with cell growth tests in Petri dishes containing 2% YPD medium and flocculation on slides with methylene blue. Yeasts showed loss of viability and the FLE strain showed greater sensitivity, the cellular growth of this yeast was also more affected and, consequently, presented higher flocculation rates. The data show that the longer exposure time and the doses and concentrations of 2,4-D interfered with the physiological response of the FLE yeast. Thus, we can suggest that this microorganism has the potential to be considered for environmental tests and analyses as a bioindicator. |
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Physiological response to the action of the agrotoxic 2,4-Dichlorophenoxiacetic acid in Saccharomyces cerevisiae Respuesta fisiológica a la acción del ácido 2,4-Diclorofenoxiacético agrotoxico en Saccharomyces cerevisiae Resposta fisiológica da ação do agrotóxico ácido 2,4-Diclorofenoxiacético em Saccharomyces cerevisiaePerfil fenotípicoToxicidadeBioindicador.Perfil fenotípicoToxicidadBioindicador.Phenotypic profileToxicity,Bioindicator.The presence of xenobiotic compounds in the environment is responsible for impacts on the ecosystem. An example is pesticides that pose risks to non-target species, such as microorganisms present in the soil and that are responsible for cycling nutrients, some can be used to measure the effects of these compounds, such as yeasts because when exposed to toxic substances begin to present changes in physiological and genetic mechanisms. Thus, this study aims to evaluate the toxicity effect of the 2,4-D pesticide on Pedra-2 (PE-2) and Fleischmann® (FLE) yeasts. 2,4-D dilutions of 2.0; 4.0 and 6.0 µg L-1 were added to a solution prepared with 20 ml of ultra-pure water and 2.0 g of sucrose where the yeasts were grown. The phenotypic profile of yeasts against the toxicity of the compound was evaluated quantitatively with cell viability. Using the methylene blue method and qualitatively with cell growth tests in Petri dishes containing 2% YPD medium and flocculation on slides with methylene blue. Yeasts showed loss of viability and the FLE strain showed greater sensitivity, the cellular growth of this yeast was also more affected and, consequently, presented higher flocculation rates. The data show that the longer exposure time and the doses and concentrations of 2,4-D interfered with the physiological response of the FLE yeast. Thus, we can suggest that this microorganism has the potential to be considered for environmental tests and analyses as a bioindicator.La presencia de compuestos xenobióticos en el medio ambiente es responsable de los impactos en el ecosistema. Un ejemplo son los pesticidas que presentan riesgos para especies no objetivo, como los microorganismos presentes en el suelo y que son responsables del ciclo de nutrientes, algunos pueden usarse para medir los efectos de estos compuestos, como las levaduras, porque cuando se exponen a sustancias tóxicas comienzan a presentar cambios en los mecanismos fisiológicos y genéticos. Así, este estudio tiene como objetivo evaluar el efecto de toxicidad del pesticida 2,4-D sobre las levaduras Pedra-2 (PE-2) y Fleischmann® (FLE). Diluciones de 2,4-D de 2,0; 4,0 y 6,0 µg L-1 que se añadieron a una solución preparada con 20 ml de agua ultrapura y 2,0 g de sacarosa donde se cultivaron las levaduras. El perfil fenotípico de las levaduras frente a la toxicidad del compuesto se evaluó cuantitativamente con viabilidad celular mediante el método del azul de metileno, y cualitativamente con pruebas de crecimiento celular en placas Petri conteniendo medio YPD al 2% y floculación en portaobjetos con azul de metileno. Las levaduras mostraron pérdida de viabilidad y la cepa FLE mostró mayor sensibilidad, el crecimiento celular de esta levadura también se vio más afectado y, en consecuencia, presentó mayores tasas de floculación. Los datos muestran que el mayor tiempo de exposición y las dosis y concentraciones de 2,4-D interfirieron con la respuesta fisiológica de la levadura FLE. Por tanto, podemos sugerir que este microorganismo tiene potencial para ser considerado como bioindicador para pruebas y análisis ambientales.A presença de compostos xenobióticos no meio ambiente são responsáveis por impactos ao ecossistema. Um exemplo são os agrotóxicos que representam riscos para as espécies não-alvos, como os microrganismos presentes no solo e que são responsáveis pela ciclagem dos nutrientes, alguns podem ser utilizados para mensurar os efeitos destes compostos como as leveduras, pois quando exposta a substância tóxicas passam a apresentar alterações nos mecanismos fisiológicos e genéticos. Assim, este estudo visa avaliar o efeito de toxicidade do agrotóxico 2,4-D nas leveduras Pedra-2 (PE-2) e Fleischmann® (FLE). Foram feitas diluições do 2,4-D de 2,0; 4,0 e 6,0 µg L-1 que foram adicionadas a uma solução preparadas com 20 mL de água ultrapura e 2,0 g de sacarose onde foram crescidas as leveduras. O perfil fenotípico das leveduras frente a toxicidade do composto foi avaliado de forma quantitativa com a viabilidade celular utilizando método azul de metileno e, qualitativa com testes de crescimento celular em placas de Petri contendo meio YPD 2% e floculação em lâminas com azul de metileno. As leveduras apresentaram perda de viabilidade e a linhagem FLE mostrou maior sensibilidade, também o crescimento celular desta levedura foi mais afetado e por consequência apresentou índices mais elevados de floculação. Os dados mostram que o tempo mais prolongado de exposição e as doses mais concentrações do 2,4-D interferiram na resposta fisiológica da levedura FLE. Desta forma, podemos sugerir que este microrganismo apresenta um potencial a ser considerado para testes e análises ambientais como um bioindicador.Research, Society and Development2021-09-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1991210.33448/rsd-v10i11.19912Research, Society and Development; Vol. 10 No. 11; e408101119912Research, Society and Development; Vol. 10 Núm. 11; e408101119912Research, Society and Development; v. 10 n. 11; e4081011199122525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/19912/17700Copyright (c) 2021 Débora Tavares Sarabia; Larissa Pires Mueller; Maria do Socorro Mascarenhas Santos; Margareth Batistotehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSarabia, Débora TavaresMueller, Larissa Pires Santos, Maria do Socorro MascarenhasBatistote, Margareth2021-10-23T19:01:11Zoai:ojs.pkp.sfu.ca:article/19912Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:39:42.011311Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Physiological response to the action of the agrotoxic 2,4-Dichlorophenoxiacetic acid in Saccharomyces cerevisiae Respuesta fisiológica a la acción del ácido 2,4-Diclorofenoxiacético agrotoxico en Saccharomyces cerevisiae Resposta fisiológica da ação do agrotóxico ácido 2,4-Diclorofenoxiacético em Saccharomyces cerevisiae |
title |
Physiological response to the action of the agrotoxic 2,4-Dichlorophenoxiacetic acid in Saccharomyces cerevisiae |
spellingShingle |
Physiological response to the action of the agrotoxic 2,4-Dichlorophenoxiacetic acid in Saccharomyces cerevisiae Sarabia, Débora Tavares Perfil fenotípico Toxicidade Bioindicador. Perfil fenotípico Toxicidad Bioindicador. Phenotypic profile Toxicity, Bioindicator. |
title_short |
Physiological response to the action of the agrotoxic 2,4-Dichlorophenoxiacetic acid in Saccharomyces cerevisiae |
title_full |
Physiological response to the action of the agrotoxic 2,4-Dichlorophenoxiacetic acid in Saccharomyces cerevisiae |
title_fullStr |
Physiological response to the action of the agrotoxic 2,4-Dichlorophenoxiacetic acid in Saccharomyces cerevisiae |
title_full_unstemmed |
Physiological response to the action of the agrotoxic 2,4-Dichlorophenoxiacetic acid in Saccharomyces cerevisiae |
title_sort |
Physiological response to the action of the agrotoxic 2,4-Dichlorophenoxiacetic acid in Saccharomyces cerevisiae |
author |
Sarabia, Débora Tavares |
author_facet |
Sarabia, Débora Tavares Mueller, Larissa Pires Santos, Maria do Socorro Mascarenhas Batistote, Margareth |
author_role |
author |
author2 |
Mueller, Larissa Pires Santos, Maria do Socorro Mascarenhas Batistote, Margareth |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Sarabia, Débora Tavares Mueller, Larissa Pires Santos, Maria do Socorro Mascarenhas Batistote, Margareth |
dc.subject.por.fl_str_mv |
Perfil fenotípico Toxicidade Bioindicador. Perfil fenotípico Toxicidad Bioindicador. Phenotypic profile Toxicity, Bioindicator. |
topic |
Perfil fenotípico Toxicidade Bioindicador. Perfil fenotípico Toxicidad Bioindicador. Phenotypic profile Toxicity, Bioindicator. |
description |
The presence of xenobiotic compounds in the environment is responsible for impacts on the ecosystem. An example is pesticides that pose risks to non-target species, such as microorganisms present in the soil and that are responsible for cycling nutrients, some can be used to measure the effects of these compounds, such as yeasts because when exposed to toxic substances begin to present changes in physiological and genetic mechanisms. Thus, this study aims to evaluate the toxicity effect of the 2,4-D pesticide on Pedra-2 (PE-2) and Fleischmann® (FLE) yeasts. 2,4-D dilutions of 2.0; 4.0 and 6.0 µg L-1 were added to a solution prepared with 20 ml of ultra-pure water and 2.0 g of sucrose where the yeasts were grown. The phenotypic profile of yeasts against the toxicity of the compound was evaluated quantitatively with cell viability. Using the methylene blue method and qualitatively with cell growth tests in Petri dishes containing 2% YPD medium and flocculation on slides with methylene blue. Yeasts showed loss of viability and the FLE strain showed greater sensitivity, the cellular growth of this yeast was also more affected and, consequently, presented higher flocculation rates. The data show that the longer exposure time and the doses and concentrations of 2,4-D interfered with the physiological response of the FLE yeast. Thus, we can suggest that this microorganism has the potential to be considered for environmental tests and analyses as a bioindicator. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-05 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/19912 10.33448/rsd-v10i11.19912 |
url |
https://rsdjournal.org/index.php/rsd/article/view/19912 |
identifier_str_mv |
10.33448/rsd-v10i11.19912 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/19912/17700 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 11; e408101119912 Research, Society and Development; Vol. 10 Núm. 11; e408101119912 Research, Society and Development; v. 10 n. 11; e408101119912 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052818783207424 |